FastQCFastQC Report
Fri 10 Feb 2017
ERR1147324.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147324.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1775305
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110640.6232168556952186No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64180.3615153452505344No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62840.3539673464559611No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21470.12093696576081292No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20090.11316365356938668No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA81950.048.7990151
GGTATCA47250.045.534741
AACGCAG101250.039.5898676
ATCAACG108650.036.6745033
TCAACGC109350.036.494124
CAACGCA111300.035.908175
TATCAAC113500.035.2093352
ACGCAGA115600.034.623937
CGCAGAG117400.033.9917538
GTACATG112150.033.6920551
GTCTAAT3600.033.1104431
TACATGG113350.033.1016922
ACATGGG114550.032.0857733
GCAGAGT129100.031.0033059
CATGGGG55650.028.9616474
ATGGGGG23750.027.2949335
GTATAGG3750.027.0181221
AGAGTAC124900.026.68900310-11
TATAACG1608.680339E-626.056092
GTATAAC4100.024.7116971
Produced by FastQC (version 0.11.5)