FastQCFastQC Report
Fri 10 Feb 2017
ERR1147323.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147323.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1025929
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81490.7943044791598639No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48070.4685509426090889No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46700.45519719200841385No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15950.15546884823413706No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11640.11345814378967746No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA70750.042.465541
AACGCAG78300.038.6684046
GGTATCA44150.037.4008641
ACGCAGA89650.033.971917
CGCAGAG90650.033.4659088
TCAACGC89950.033.3295634
CGTATAG902.366573E-433.117691
ATCAACG90750.033.1012953
CAACGCA91250.032.919925
TATCAAC92150.032.642952
TAGGTCG1102.0433094E-532.4458275
CATGGGT23450.031.7078974
GTACATG81450.031.3245811
GCAGAGT97700.031.051029
ACATGGG84000.030.3085213
TACATGG84600.030.0642832
TATAACG1004.409884E-429.782652
ATGGGGG9550.026.7833795
AGAGTAC95100.026.39564110-11
CATGGGG35700.025.8263914
Produced by FastQC (version 0.11.5)