FastQCFastQC Report
Fri 10 Feb 2017
ERR1147322.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147322.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1097561
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67670.6165488751878028No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41050.37401110279975325No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38310.34904665891007425No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13050.11889999735777783No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11930.10869555314009882No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA51250.046.2690661
GGTATCA30850.042.68141
GGGTTAG4000.038.7270971
AACGCAG62150.037.9977576
ATCAACG67300.035.0900543
TCAACGC67350.035.0644
CAACGCA68950.034.0777855
TATCAAC69950.033.9617162
GTACATG70700.033.7929841
TACATGG71950.033.4314352
ACGCAGA70700.033.4025547
CATGGGT17100.033.0473064
CGCAGAG72850.032.4984058
ACATGGG73150.032.2838063
GCAGAGT78400.030.4254389
GGTTAGA4200.029.76972
CATGGGG37450.028.5909924
ATGGGTG11450.027.534615
AGAGTAC76650.026.07568410-11
ATGGGGG17150.026.0139165
Produced by FastQC (version 0.11.5)