##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1143040_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2391 Sequences flagged as poor quality 0 Sequence length 125 %GC 49 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 21.624843161856962 14.0 14.0 33.0 14.0 33.0 2 22.52572145545797 27.0 14.0 33.0 14.0 33.0 3 22.631953157674612 27.0 14.0 33.0 14.0 33.0 4 22.40401505646173 27.0 14.0 33.0 14.0 33.0 5 22.475533249686325 27.0 14.0 33.0 14.0 33.0 6 23.723546633207864 27.0 14.0 37.0 14.0 37.0 7 23.76871601840234 27.0 14.0 33.0 14.0 37.0 8 23.73943956503555 14.0 14.0 37.0 14.0 37.0 9 23.855708908406523 27.0 14.0 37.0 14.0 37.0 10-11 23.726056043496445 24.5 14.0 35.0 14.0 37.0 12-13 23.65098285236303 27.0 14.0 35.0 14.0 37.0 14-15 23.445838561271433 20.5 14.0 33.0 14.0 37.0 16-17 23.677122542869093 27.0 14.0 35.0 14.0 37.0 18-19 23.45315767461313 24.5 14.0 33.0 14.0 37.0 20-21 23.35069008782936 18.0 14.0 33.0 14.0 37.0 22-23 23.18485989125889 18.0 14.0 33.0 14.0 37.0 24-25 23.045378502718528 14.0 14.0 33.0 14.0 37.0 26-27 23.049769970723545 18.0 14.0 33.0 14.0 37.0 28-29 22.9788791300711 14.0 14.0 33.0 14.0 37.0 30-31 22.90840652446675 14.0 14.0 33.0 14.0 37.0 32-33 22.940192388122124 14.0 14.0 33.0 14.0 37.0 34-35 22.878084483479718 14.0 14.0 33.0 14.0 37.0 36-37 23.101003764115433 18.0 14.0 33.0 14.0 37.0 38-39 23.00564617314931 18.0 14.0 33.0 14.0 37.0 40-41 22.76056043496445 14.0 14.0 33.0 14.0 37.0 42-43 22.893559180259306 14.0 14.0 33.0 14.0 37.0 44-45 22.675031367628605 14.0 14.0 33.0 14.0 37.0 46-47 22.740485152655793 14.0 14.0 33.0 14.0 37.0 48-49 22.8021748222501 14.0 14.0 33.0 14.0 37.0 50-51 22.941237975742368 14.0 14.0 33.0 14.0 37.0 52-53 22.710999581764952 14.0 14.0 33.0 14.0 37.0 54-55 22.829360100376412 14.0 14.0 33.0 14.0 37.0 56-57 22.66081137599331 14.0 14.0 33.0 14.0 37.0 58-59 22.66980342952739 14.0 14.0 33.0 14.0 37.0 60-61 22.591802593057295 14.0 14.0 33.0 14.0 37.0 62-63 22.532204098703474 14.0 14.0 33.0 14.0 37.0 64-65 22.67754077791719 14.0 14.0 33.0 14.0 37.0 66-67 22.513383521539104 14.0 14.0 33.0 14.0 37.0 68-69 22.47051442910916 14.0 14.0 33.0 14.0 37.0 70-71 22.429945629443747 14.0 14.0 33.0 14.0 37.0 72-73 22.203053115851105 14.0 14.0 33.0 14.0 37.0 74-75 22.279381012128816 14.0 14.0 33.0 14.0 37.0 76-77 22.24362191551652 14.0 14.0 33.0 14.0 37.0 78-79 22.28293601003764 14.0 14.0 30.0 14.0 37.0 80-81 22.106440819740694 14.0 14.0 33.0 14.0 37.0 82-83 22.039941447093266 14.0 14.0 30.0 14.0 37.0 84-85 21.93245503973233 14.0 14.0 27.0 14.0 37.0 86-87 21.958803847762443 14.0 14.0 30.0 14.0 37.0 88-89 21.747386030949393 14.0 14.0 27.0 14.0 37.0 90-91 21.6978251777499 14.0 14.0 30.0 14.0 37.0 92-93 21.648264324550397 14.0 14.0 27.0 14.0 37.0 94-95 21.631534922626518 14.0 14.0 27.0 14.0 37.0 96-97 21.35612714345462 14.0 14.0 27.0 14.0 37.0 98-99 21.32831451275617 14.0 14.0 27.0 14.0 37.0 100-101 21.411961522375577 14.0 14.0 27.0 14.0 37.0 102-103 21.296737766624844 14.0 14.0 27.0 14.0 37.0 104-105 21.048306148055207 14.0 14.0 27.0 14.0 37.0 106-107 20.882266833960685 14.0 14.0 27.0 14.0 37.0 108-109 20.928900041823503 14.0 14.0 27.0 14.0 37.0 110-111 21.060225846925974 14.0 14.0 27.0 14.0 37.0 112-113 20.795483061480553 14.0 14.0 27.0 14.0 37.0 114-115 20.663529903805937 14.0 14.0 27.0 14.0 37.0 116-117 20.606231702216647 14.0 14.0 27.0 14.0 37.0 118-119 20.693851944792975 14.0 14.0 27.0 14.0 37.0 120-121 20.40757005437056 14.0 14.0 27.0 14.0 37.0 122-123 20.323923044751147 14.0 14.0 27.0 14.0 37.0 124-125 19.759723964868257 14.0 14.0 22.0 14.0 35.0 >>END_MODULE >>Per sequence quality scores fail #Quality Count 9 1.0 10 0.0 11 0.0 12 0.0 13 0.0 14 30.0 15 229.0 16 279.0 17 301.0 18 236.0 19 222.0 20 156.0 21 107.0 22 67.0 23 57.0 24 42.0 25 49.0 26 60.0 27 36.0 28 39.0 29 48.0 30 37.0 31 37.0 32 35.0 33 42.0 34 45.0 35 83.0 36 153.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 27.938101212881637 23.755750731911334 21.9573400250941 26.348808030112924 2 17.824267782426777 27.447698744769873 30.502092050209207 24.22594142259414 3 18.166596902469653 27.54290498116367 26.831310171619926 27.459187944746756 4 16.45039765592298 25.0732524068648 28.296358308915863 30.17999162829636 5 18.668899120971115 30.933444956048557 28.50565089995814 21.892005023022186 6 23.514644351464433 30.418410041841003 23.640167364016737 22.426778242677827 7 22.18501465048137 30.096274591879446 24.445374633737966 23.273336123901213 8 21.96652719665272 30.711297071129707 23.598326359832637 23.723849372384937 9 22.217573221757323 24.560669456066947 23.347280334728033 29.8744769874477 10-11 20.81589958158996 28.05439330543933 24.93723849372385 26.19246861924686 12-13 21.10878661087866 26.464435146443517 25.523012552301257 26.90376569037657 14-15 21.058134671685487 26.82977833542451 24.843161856963615 27.26892513592639 16-17 20.799330403850178 27.181418706842436 25.65390249006068 26.365348399246702 18-19 21.02510460251046 26.924686192468616 26.317991631799163 25.732217573221757 20-21 21.9201003974064 26.0405772850868 25.287596737084293 26.75172558042251 22-23 22.087429408073625 26.58439656975528 24.701945199749005 26.62622882242209 24-25 20.999790838736665 26.31248692742104 25.224848358084085 27.46287387575821 26-27 22.040987034713506 26.097867001254706 24.173985780008366 27.687160184023423 28-29 21.727310748640736 26.076955248849853 24.90589711417817 27.289836888331244 30-31 21.56452624973855 26.75172558042251 24.53461618908178 27.14913198075716 32-33 22.38025517674127 25.308512863417693 24.409119431081365 27.902112528759677 34-35 21.694560669456067 26.10878661087866 24.560669456066947 27.635983263598323 36-37 21.941016523739805 25.852332148086177 24.26270654674754 27.943944781426477 38-39 23.02865509307676 23.321480861744405 24.701945199749005 28.947918845429825 40-41 22.003764902740013 24.241790420414137 24.555532315415185 29.19891236143066 42-43 21.522693997071745 25.831416021752773 24.325454925747753 28.320435055427733 44-45 22.19201003974064 24.890190336749633 24.72286132608241 28.19493829742732 46-47 20.790629575402637 25.12026772641707 25.07843547375026 29.010667224430037 48-49 21.878268144739593 25.726835390085757 24.220874294080737 28.174022171093917 50-51 20.376569037656903 25.94142259414226 25.481171548117153 28.20083682008368 52-53 21.125287596737085 26.166074043087225 24.283622673080945 28.425015687094753 54-55 22.15481171548117 24.10041841004184 25.271966527196653 28.472803347280333 56-57 21.552626072400084 25.298179535467668 24.27286043105252 28.876333961079727 58-59 22.624529091670155 25.17789870238594 24.445374633737966 27.752197572205944 60-61 21.88284518828452 25.94142259414226 24.539748953974897 27.635983263598323 62-63 21.66178317287568 25.491837588949352 24.947676852239432 27.898702385935536 64-65 21.90376569037657 24.85355648535565 24.623430962343097 28.619246861924687 66-67 22.280334728033473 26.00418410041841 23.93305439330544 27.78242677824268 68-69 22.322175732217573 25.460251046025107 24.288702928870293 27.92887029288703 70-71 23.158995815899583 24.267782426778243 23.661087866108787 28.912133891213387 72-73 22.301255230125523 24.560669456066947 25.606694560669457 27.531380753138073 74-75 22.07112970711297 25.334728033472803 24.372384937238493 28.221757322175733 76-77 22.615062761506277 25.167364016736403 24.811715481171547 27.405857740585777 78-79 23.723849372384937 24.8326359832636 24.12133891213389 27.322175732217573 80-81 22.238493723849373 26.04602510460251 24.435146443514643 27.280334728033473 82-83 24.184100418410043 25.418410041841007 22.531380753138073 27.866108786610877 84-85 22.845188284518827 25.125523012552303 24.267782426778243 27.761506276150627 86-87 24.163179916317993 26.84100418410042 23.598326359832637 25.397489539748953 88-89 22.552301255230127 25.64853556485356 23.619246861924687 28.179916317991633 90-91 22.719665271966527 26.04602510460251 23.305439330543933 27.92887029288703 92-93 21.2596777568529 26.156099602427286 24.377484829462233 28.206737811257586 94-95 23.284518828451883 26.673640167364017 23.84937238493724 26.19246861924686 96-97 22.824267782426777 26.79916317991632 23.84937238493724 26.527196652719663 98-99 21.506276150627617 26.40167364016736 23.80753138075314 28.284518828451883 100-101 23.556485355648533 25.230125523012553 22.740585774058577 28.472803347280333 102-103 22.80334728033473 27.19665271966527 22.217573221757323 27.78242677824268 104-105 22.301255230125523 26.861924686192467 23.640167364016737 27.19665271966527 106-107 23.138075313807533 27.15481171548117 23.117154811715483 26.589958158995813 108-109 22.15481171548117 27.405857740585777 23.20083682008368 27.238493723849373 110-111 22.698744769874477 27.19665271966527 23.117154811715483 26.98744769874477 112-113 22.510460251046023 26.84100418410042 23.451882845188283 27.19665271966527 114-115 22.17573221757322 28.05439330543933 22.364016736401673 27.405857740585777 116-117 22.00836820083682 28.05439330543933 22.510460251046023 27.426778242677823 118-119 22.217573221757323 28.221757322175733 22.15481171548117 27.405857740585777 120-121 22.635983263598327 26.610878661087867 22.635983263598327 28.117154811715483 122-123 24.037656903765694 25.815899581589957 22.343096234309623 27.803347280334727 124-125 21.74094998953756 26.762921113203596 22.870893492362416 28.625235404896422 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 6.0 1 3.5 2 1.5 3 1.0 4 0.5 5 1.0 6 1.0 7 1.0 8 0.5 9 1.0 10 1.0 11 0.0 12 1.0 13 1.0 14 0.0 15 2.5 16 3.0 17 3.0 18 4.0 19 3.0 20 2.5 21 2.0 22 3.5 23 6.0 24 6.0 25 6.5 26 8.0 27 10.0 28 13.0 29 12.5 30 12.5 31 17.5 32 16.5 33 18.0 34 28.0 35 31.0 36 36.5 37 42.0 38 48.0 39 45.5 40 41.0 41 46.0 42 50.5 43 65.0 44 71.0 45 69.0 46 62.5 47 74.5 48 90.0 49 89.0 50 101.0 51 106.5 52 102.0 53 92.0 54 90.0 55 105.5 56 96.0 57 70.0 58 66.5 59 70.5 60 59.5 61 55.5 62 57.0 63 44.0 64 37.5 65 35.0 66 26.5 67 19.5 68 18.0 69 14.0 70 9.5 71 11.0 72 11.5 73 8.5 74 6.0 75 4.5 76 4.5 77 3.5 78 2.0 79 1.5 80 0.5 81 1.5 82 1.5 83 0.0 84 0.0 85 0.0 86 0.5 87 0.5 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.04182350480970305 3 0.0836470096194061 4 0.0836470096194061 5 0.0836470096194061 6 0.04182350480970305 7 0.0836470096194061 8 0.04182350480970305 9 0.04182350480970305 10-11 0.04182350480970305 12-13 0.04182350480970305 14-15 0.0 16-17 0.06273525721455457 18-19 0.04182350480970305 20-21 0.020911752404851526 22-23 0.020911752404851526 24-25 0.020911752404851526 26-27 0.0 28-29 0.0 30-31 0.020911752404851526 32-33 0.020911752404851526 34-35 0.04182350480970305 36-37 0.020911752404851526 38-39 0.020911752404851526 40-41 0.020911752404851526 42-43 0.020911752404851526 44-45 0.020911752404851526 46-47 0.020911752404851526 48-49 0.020911752404851526 50-51 0.04182350480970305 52-53 0.020911752404851526 54-55 0.04182350480970305 56-57 0.06273525721455457 58-59 0.0836470096194061 60-61 0.04182350480970305 62-63 0.0836470096194061 64-65 0.04182350480970305 66-67 0.04182350480970305 68-69 0.04182350480970305 70-71 0.04182350480970305 72-73 0.04182350480970305 74-75 0.04182350480970305 76-77 0.04182350480970305 78-79 0.04182350480970305 80-81 0.04182350480970305 82-83 0.04182350480970305 84-85 0.04182350480970305 86-87 0.04182350480970305 88-89 0.04182350480970305 90-91 0.04182350480970305 92-93 0.06273525721455457 94-95 0.04182350480970305 96-97 0.04182350480970305 98-99 0.04182350480970305 100-101 0.04182350480970305 102-103 0.04182350480970305 104-105 0.04182350480970305 106-107 0.04182350480970305 108-109 0.04182350480970305 110-111 0.04182350480970305 112-113 0.04182350480970305 114-115 0.04182350480970305 116-117 0.04182350480970305 118-119 0.04182350480970305 120-121 0.04182350480970305 122-123 0.04182350480970305 124-125 0.06273525721455457 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2391.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 99.45629443747386 #Duplication Level Percentage of deduplicated Percentage of total 1 99.78973927670312 99.24717691342535 2 0.1261564339781329 0.2509410288582183 3 0.04205214465937763 0.12547051442910914 4 0.0 0.0 5 0.0 0.0 6 0.0 0.0 7 0.0 0.0 8 0.0 0.0 9 0.04205214465937763 0.37641154328732745 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 9 0.37641154328732745 No Hit ATCTAGAGTTATCCAACCACCCACAGCACCATTCACGAAGTATCCATTGT 3 0.12547051442910914 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.04182350480970305 0.0 30-31 0.0 0.0 0.0 0.04182350480970305 0.0 32-33 0.0 0.0 0.0 0.06273525721455459 0.0 34-35 0.0 0.0 0.0 0.0836470096194061 0.0 36-37 0.0 0.0 0.0 0.1672940192388122 0.0 38-39 0.0 0.0 0.0 0.2718527812630699 0.0 40-41 0.0 0.0 0.0 0.35549979088247596 0.0 42-43 0.0 0.0 0.0 0.43914680050188204 0.0 44-45 0.0 0.0 0.0 0.5018820577164367 0.0 46-47 0.0 0.0 0.0 0.5018820577164367 0.0 48-49 0.0 0.0 0.0 0.5855290673358428 0.0 50-51 0.0 0.0 0.0 0.6064408197406943 0.0 52-53 0.0 0.0 0.0 0.7109995817649519 0.0 54-55 0.0 0.0 0.0 0.8364700961940611 0.0 56-57 0.0 0.0 0.0 0.8992053534086156 0.0 58-59 0.0 0.0 0.0 1.0037641154328734 0.0 60-61 0.0 0.0 0.0 1.1292346298619824 0.0 62-63 0.0 0.0 0.0 1.2965286491007948 0.0 64-65 0.0 0.0 0.0 1.4010874111250522 0.0 66-67 0.0 0.0 0.0 1.5265579255541613 0.0 68-69 0.0 0.0 0.0 1.5683814303638646 0.0 70-71 0.0 0.0 0.0 1.6102049351735674 0.0 72-73 0.0 0.0 0.0 1.631116687578419 0.0 74-75 0.0 0.0 0.0 1.631116687578419 0.0 76-77 0.0 0.0 0.0 1.6729401923881222 0.0 78-79 0.0 0.0 0.0 1.8193224592220827 0.0 80-81 0.0 0.0 0.0 1.8820577164366374 0.0 82-83 0.0 0.0 0.0 2.0075282308657467 0.0 84-85 0.0 0.0 0.0 2.132998745294856 0.0 86-87 0.0 0.0 0.0 2.1748222501045587 0.0 88-89 0.0 0.0 0.0 2.1748222501045587 0.0 90-91 0.0 0.0 0.0 2.216645754914262 0.0 92-93 0.0 0.0 0.0 2.2793810121288165 0.0 94-95 0.0 0.0 0.0 2.383939774153074 0.0 96-97 0.0 0.0 0.0 2.4675867837724805 0.0 98-99 0.0 0.0 0.0 2.5512337933918863 0.0 100-101 0.0 0.0 0.0 2.634880803011292 0.0 102-103 0.0 0.0 0.0 2.781263069845253 0.0 104-105 0.0 0.0 0.0 2.8439983270598077 0.0 106-107 0.0 0.0 0.0 2.9067335842743622 0.0 108-109 0.0 0.0 0.0 2.990380593893768 0.0 110-111 0.0 0.0 0.0 3.053115851108323 0.0 112-113 0.0 0.0 0.0 3.1158511083228775 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE