FastQCFastQC Report
Sun 1 Jan 2017
ERR1142928_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1142928_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences517970
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18320.3536884375542985No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17750.032.8676071
GTATCAA22200.025.9985522
GTAAGTA2451.6159902E-824.2864652
TATAGTG1300.002051427722.883115
AACGCAG25650.022.492977
CAACGCA25250.022.3781746
TCAACGC25900.022.2823185
TATCAAC26250.022.2140183
ATCAACG26350.021.9039024
GTATACG1653.2008192E-421.6474971
ATGGGGG11700.021.3575695
CCTATGC1450.003872517320.5099438
ACGCAGA28050.020.3563928
CATGGGG19150.020.1964454
CGCAGAG29050.019.8604039
TGGGGGG7200.019.8262796
ATACCGT3051.6469494E-719.5012576
TACCGTC2158.5739186E-519.3652027
ATATACG1856.964892E-419.2904346
GTCTACA3452.8612703E-818.9807781
Produced by FastQC (version 0.11.5)