##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1142928_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	517970
Sequences flagged as poor quality	0
Sequence length	125
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.634978859779523	33.0	33.0	33.0	27.0	33.0
2	31.63645770990598	33.0	33.0	33.0	27.0	33.0
3	31.66278162827963	33.0	33.0	33.0	33.0	33.0
4	31.635123655810183	33.0	33.0	33.0	33.0	33.0
5	31.684047338648956	33.0	33.0	33.0	33.0	33.0
6	35.133471050446936	37.0	37.0	37.0	33.0	37.0
7	35.09445913856015	37.0	37.0	37.0	33.0	37.0
8	35.114489256134526	37.0	37.0	37.0	33.0	37.0
9	35.03944243875128	37.0	37.0	37.0	33.0	37.0
10-11	35.048857076664675	37.0	37.0	37.0	33.0	37.0
12-13	35.035722146070235	37.0	37.0	37.0	33.0	37.0
14-15	35.02722454968435	37.0	37.0	37.0	33.0	37.0
16-17	35.04176882831052	37.0	37.0	37.0	33.0	37.0
18-19	35.05604185570593	37.0	37.0	37.0	33.0	37.0
20-21	35.08145838562079	37.0	37.0	37.0	33.0	37.0
22-23	35.148512462111704	37.0	37.0	37.0	33.0	37.0
24-25	35.175434870745406	37.0	37.0	37.0	33.0	37.0
26-27	35.23324613394598	37.0	37.0	37.0	33.0	37.0
28-29	35.28728304728073	37.0	37.0	37.0	33.0	37.0
30-31	35.296096299013456	37.0	37.0	37.0	33.0	37.0
32-33	35.28918277120297	37.0	37.0	37.0	33.0	37.0
34-35	35.27217792536247	37.0	37.0	37.0	33.0	37.0
36-37	35.26593914705485	37.0	37.0	37.0	33.0	37.0
38-39	35.25180898507636	37.0	37.0	37.0	33.0	37.0
40-41	35.2369905592988	37.0	37.0	37.0	33.0	37.0
42-43	35.22273008089272	37.0	37.0	37.0	33.0	37.0
44-45	35.20680155221345	37.0	37.0	37.0	33.0	37.0
46-47	35.18510338436589	37.0	37.0	37.0	33.0	37.0
48-49	35.18600594629032	37.0	37.0	37.0	33.0	37.0
50-51	35.181499893816245	37.0	37.0	37.0	33.0	37.0
52-53	35.14904048497017	37.0	37.0	37.0	33.0	37.0
54-55	35.122293762187	37.0	37.0	37.0	33.0	37.0
56-57	35.072661544104875	37.0	37.0	37.0	33.0	37.0
58-59	35.07170878622314	37.0	37.0	37.0	33.0	37.0
60-61	35.025921385408424	37.0	37.0	37.0	30.0	37.0
62-63	35.014555862308626	37.0	37.0	37.0	27.0	37.0
64-65	35.006464660115455	37.0	37.0	37.0	27.0	37.0
66-67	34.969504025329655	37.0	37.0	37.0	27.0	37.0
68-69	34.94208255304362	37.0	37.0	37.0	27.0	37.0
70-71	34.89086047454486	37.0	37.0	37.0	27.0	37.0
72-73	34.861028631001794	37.0	37.0	37.0	27.0	37.0
74-75	34.861949533756786	37.0	37.0	37.0	27.0	37.0
76-77	34.78748865764427	37.0	37.0	37.0	27.0	37.0
78-79	34.726238971369	37.0	37.0	37.0	27.0	37.0
80-81	34.70012355927949	37.0	37.0	37.0	27.0	37.0
82-83	34.646871440430914	37.0	37.0	37.0	27.0	37.0
84-85	34.594284418016485	37.0	37.0	37.0	27.0	37.0
86-87	34.52611444678263	37.0	37.0	37.0	27.0	37.0
88-89	34.379608857655846	37.0	37.0	37.0	27.0	37.0
90-91	34.34373901963434	37.0	37.0	37.0	27.0	37.0
92-93	34.32025986060969	37.0	37.0	37.0	27.0	37.0
94-95	34.21827325906906	37.0	37.0	37.0	27.0	37.0
96-97	34.06911500666062	37.0	37.0	37.0	24.5	37.0
98-99	33.99281715157248	37.0	37.0	37.0	22.0	37.0
100-101	33.94295712106879	37.0	37.0	37.0	22.0	37.0
102-103	33.88631098326158	37.0	37.0	37.0	22.0	37.0
104-105	33.801694113558696	37.0	37.0	37.0	22.0	37.0
106-107	33.651001023225284	37.0	33.0	37.0	22.0	37.0
108-109	33.48136764677491	37.0	33.0	37.0	22.0	37.0
110-111	33.423305403787865	37.0	33.0	37.0	22.0	37.0
112-113	33.31502403614109	37.0	33.0	37.0	22.0	37.0
114-115	33.23632835878526	37.0	33.0	37.0	18.0	37.0
116-117	33.09385775237948	37.0	33.0	37.0	14.0	37.0
118-119	32.88241017819565	37.0	33.0	37.0	14.0	37.0
120-121	32.849973936714484	37.0	33.0	37.0	14.0	37.0
122-123	32.726961986215414	37.0	33.0	37.0	14.0	37.0
124-125	30.973150954688492	35.0	27.5	37.0	14.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	417.0
1	49.0
2	29.0
3	21.0
4	27.0
5	31.0
6	31.0
7	16.0
8	22.0
9	31.0
10	21.0
11	23.0
12	25.0
13	19.0
14	21.0
15	86.0
16	211.0
17	444.0
18	1075.0
19	1974.0
20	2739.0
21	3406.0
22	3802.0
23	4229.0
24	4767.0
25	5179.0
26	5988.0
27	7006.0
28	8160.0
29	9933.0
30	12232.0
31	14718.0
32	18383.0
33	24003.0
34	35732.0
35	68270.0
36	284850.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.47600615756649	20.011704784475516	13.154362156847977	24.357926901110023
2	17.4225483052338	22.28196688359004	35.9477528426497	24.34773196852646
3	19.775354753895527	25.936472953640337	28.33874647179368	25.949425820670456
4	14.998279082538296	19.195964158510034	33.161886125538025	32.64387063341364
5	15.813137681103367	37.01103057775165	31.05656845378528	16.1192632873597
6	31.11557866656357	34.940557885987126	17.762463513173916	16.181399934275387
7	27.30268498403544	32.598821792205584	20.895600275224393	19.202892948534583
8	25.859956557700187	34.24269713296281	20.16781714038356	19.72952916895344
9	26.092663256745418	18.648828371163358	20.047978597138293	35.21052977495293
10-11	25.663096777435673	26.89554933192625	25.467207078701854	21.974146811936226
12-13	25.900100317187874	25.18695915215547	25.340817498100932	23.57212303255573
14-15	24.467610130966555	26.140545513583724	23.114843133919873	26.27700122152985
16-17	24.24141976609114	28.16406091449048	24.124871226426947	23.46964809299143
18-19	24.290491239260483	27.255129357416912	25.40198892464701	23.052390478675598
20-21	25.575068664193918	26.05132080723192	24.887846872565827	23.485763656008334
22-23	25.451672449285308	25.833325279546926	24.66294903886113	24.052053232306637
24-25	24.619132377468176	26.216621540400087	24.755670738659973	24.408575343471767
26-27	24.33470364378886	26.484873434724708	24.880315268041617	24.30010765344481
28-29	24.294606470766166	26.63907295968117	25.09352256573155	23.972798003821115
30-31	24.95264089816053	26.137101700288408	25.02000680424647	23.89025059730459
32-33	24.527266049370784	26.20150393381145	24.721926891032457	24.54930312578531
34-35	24.572102442160006	26.487649040390128	24.88924975955381	24.050998757896057
36-37	24.582706196355016	26.475282344559446	24.791413515998155	24.15059794308738
38-39	24.431163427404698	26.39434566392625	24.89201385934926	24.282477049319795
40-41	24.408396005603525	26.29035267593433	25.085633572420914	24.21561774604123
42-43	24.67127586146895	26.28323734066474	24.757794933365815	24.2876918645005
44-45	24.328570171800575	26.343367198474088	24.936267216552253	24.391795413173085
46-47	24.21128541433858	26.257071267550085	24.997196626671876	24.534446691439467
48-49	24.282575217825485	26.503220617364747	24.985185063450462	24.2290191013593
50-51	24.054692824104283	26.5908816293783	25.030618508368736	24.32380703814868
52-53	24.312955882424024	26.108981578603917	24.956713827889732	24.621348711082327
54-55	24.11692821730488	25.943109835799312	25.172573147811562	24.76738879908425
56-57	24.284340214507015	25.987152779456046	24.732301237837916	24.99620576819902
58-59	24.09651716090512	26.13028405256838	25.13491095200438	24.63828783452212
60-61	24.36880063419633	26.006109940254063	25.296022738258667	24.329066687290936
62-63	24.114472763270342	25.96798954381813	25.170918357482318	24.74661933542921
64-65	24.092414665225487	25.818542874766276	25.145261993661645	24.943780466346592
66-67	24.253097490585464	25.79606595733367	25.011867872629445	24.93896867945142
68-69	24.14490578880604	25.773104173756998	25.182253797840414	24.89973623959655
70-71	23.96970789897863	25.833472732951268	25.394098029679064	24.80272133839104
72-73	24.35947143038086	25.869435530421182	25.16400567360526	24.607087365592697
74-75	24.281393585915623	25.6182413094262	25.29964495119046	24.80072015346772
76-77	24.28477776574514	25.56960581073346	25.144262751589597	25.00135367193181
78-79	24.146586733707213	25.725004834654808	25.375652678398765	24.75275575323922
80-81	23.973113397755196	25.83788550856234	25.569831770170026	24.619169323512434
82-83	24.155846416263447	25.801414078122402	25.585395852914218	24.457343652699937
84-85	24.245348795604237	25.839218557045974	25.118196051202656	24.797236596147133
86-87	23.93769335436397	25.949013922964813	25.15678687719224	24.956505845478976
88-89	23.99141195073477	25.564727102162006	25.36124449344533	25.082616453657902
90-91	24.35920252875609	25.56525591274708	25.393183655940604	24.682357902556227
92-93	24.208216135909034	25.79826438191336	25.334858995539083	24.658660486638524
94-95	23.74807030847981	25.83668329070924	25.385744851254156	25.029501549556798
96-97	23.926097935806215	25.697147770278043	25.284947333705365	25.091806960210377
98-99	23.805009605581105	25.83301572684165	25.22669364870852	25.135281018868728
100-101	23.785346304081454	26.123794630466175	25.250889973975333	24.839969091477045
102-103	23.871455071947942	25.93685139011666	25.517872375819923	24.673821162115477
104-105	24.012430879108443	26.02030995950195	25.32893177815156	24.638327383238046
106-107	24.075741645814855	25.96570404968454	25.3358455453857	24.622708759114907
108-109	23.82799389077881	26.055789686481656	25.22858798255448	24.887628440185054
110-111	23.45560555119222	26.550874966630168	25.24093984918771	24.7525796329899
112-113	23.587176054539686	26.534412313216276	25.19616643580676	24.68224519643728
114-115	23.487815754827313	26.55972945181296	25.2673510983765	24.685103694983233
116-117	23.530726343458227	26.55523060357546	25.189884071795575	24.724158981170735
118-119	23.506333045328372	26.414860669132604	25.255455242964274	24.823351042574746
120-121	23.417196839181916	26.420341599736112	25.568228535388954	24.594233025693015
122-123	23.302808031127682	26.581682109902697	25.558636248425525	24.5568736105441
124-125	23.184752940630087	26.64423237871503	25.335716089506228	24.835298591148646
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1310.0
1	964.0
2	411.5
3	177.5
4	164.0
5	147.0
6	110.5
7	102.5
8	112.0
9	126.0
10	120.0
11	113.5
12	103.0
13	101.0
14	113.5
15	135.5
16	164.0
17	181.0
18	197.0
19	218.5
20	271.5
21	339.5
22	394.0
23	489.0
24	631.0
25	788.0
26	1008.0
27	1282.0
28	1635.0
29	2211.0
30	2867.0
31	3517.5
32	4362.5
33	5178.5
34	6013.5
35	6903.5
36	7875.0
37	9023.0
38	9894.5
39	10683.5
40	11226.0
41	11819.5
42	12742.5
43	14007.0
44	15554.5
45	16897.0
46	18414.0
47	19976.0
48	21719.5
49	23288.0
50	24134.5
51	23860.5
52	23189.0
53	23083.0
54	22562.0
55	21470.0
56	20366.0
57	18581.0
58	16516.5
59	14550.5
60	12466.0
61	10409.5
62	8573.5
63	6753.0
64	5024.0
65	3996.0
66	3170.0
67	2479.5
68	2022.5
69	1680.0
70	1392.5
71	1166.0
72	1006.0
73	972.0
74	830.5
75	709.0
76	581.5
77	362.0
78	233.5
79	143.5
80	91.5
81	56.5
82	40.0
83	29.5
84	18.0
85	9.0
86	6.5
87	3.5
88	0.5
89	0.0
90	0.5
91	1.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04498330019113076
2	0.11255478116493232
3	0.13707357568971176
4	0.15522134486553274
5	0.16641890456976272
6	0.12742050697916868
7	0.11081722879703458
8	0.09691680985385254
9	0.12664826148232525
10-11	0.11332702666177578
12-13	0.11796049964283646
14-15	0.11313396528756492
16-17	0.1137131494101975
18-19	0.11680213139757128
20-21	0.1152576404038844
22-23	0.11873274513967991
24-25	0.10290171245438924
26-27	0.10985192192598027
28-29	0.11226518910361603
30-31	0.12471764774021663
32-33	0.12742050697916868
34-35	0.1356256153831303
36-37	0.1425758248547213
38-39	0.1494295036392069
40-41	0.1535803231847404
42-43	0.14344460103867018
44-45	0.14894685020367973
46-47	0.14209317141919414
48-49	0.14585786821630597
50-51	0.13852153599629322
52-53	0.1491399115778906
54-55	0.15406297662026758
56-57	0.14238276348051043
58-59	0.15000868776183948
60-61	0.15058787188447206
62-63	0.1474988898970983
64-65	0.15464216074290016
66-67	0.15782767341737938
68-69	0.1610131860918586
70-71	0.16825298762476593
72-73	0.16188196227580748
74-75	0.16564665907291928
76-77	0.16564665907291928
78-79	0.16796339556344964
80-81	0.17558931984477866
82-83	0.1706662548024017
84-85	0.1788713632063633
86-87	0.18273259069058054
88-89	0.18775218642006292
90-91	0.20001158368245264
92-93	0.20001158368245264
94-95	0.20232832017298297
96-97	0.19084116840743673
98-99	0.20908546827036315
100-101	0.1854354499295326
102-103	0.1928683128366508
104-105	0.19914280749850377
106-107	0.1985636233758712
108-109	0.2020387281116667
110-111	0.20001158368245264
112-113	0.20503117941193505
114-115	0.2111126126995772
116-117	0.22037955866169856
118-119	0.23099793424329593
120-121	0.20821669208641427
122-123	0.2183524142324845
124-125	0.23196324111435024
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	517970.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	82.10599303098078
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.56259155503825	73.5362551805455
2	7.075237899509183	11.618388673392642
3	1.6772959670669143	4.131481529486647
4	0.66306760451189	2.177672964604894
5	0.3488966103360299	1.4323251328391446
6	0.19949336999655526	0.9827760747998422
7	0.12233639701857142	0.7031185952729487
8	0.06881056544886661	0.45198078457619917
9	0.05513034549887071	0.40738785869932576
>10	0.21219138400087012	2.8646578383656083
>50	0.009342689032896166	0.5102249249170988
>100	0.005338678610577775	0.7822132844442199
>500	0.0	0.0
>1k	2.6693393052888643E-4	0.4015171580559829
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1832	0.3536884375542985	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.9306137421086164E-4
2	0.0	0.0	0.0	0.0	1.9306137421086164E-4
3	0.0	0.0	0.0	0.0	1.9306137421086164E-4
4	0.0	0.0	0.0	0.0	1.9306137421086164E-4
5	0.0	0.0	0.0	1.9306137421086164E-4	1.9306137421086164E-4
6	0.0	0.0	0.0	1.9306137421086164E-4	1.9306137421086164E-4
7	0.0	0.0	0.0	1.9306137421086164E-4	1.9306137421086164E-4
8	0.0	0.0	0.0	1.9306137421086164E-4	1.9306137421086164E-4
9	0.0	0.0	0.0	1.9306137421086164E-4	1.9306137421086164E-4
10-11	0.0	0.0	0.0	1.9306137421086164E-4	1.9306137421086164E-4
12-13	0.0	0.0	0.0	4.826534355271541E-4	1.9306137421086164E-4
14-15	0.0	0.0	0.0	5.791841226325849E-4	5.791841226325849E-4
16-17	0.0	0.0	0.0	5.791841226325849E-4	5.791841226325849E-4
18-19	0.0	0.0	0.0	5.791841226325849E-4	5.791841226325849E-4
20-21	0.0	0.0	0.0	0.0011583682452651698	5.791841226325849E-4
22-23	0.0	0.0	0.0	0.002123675116319478	5.791841226325849E-4
24-25	0.0	0.0	0.0	0.005212657103693265	5.791841226325849E-4
26-27	0.0	0.0	0.0	0.011294090391335405	9.653068710543082E-4
28-29	1.9306137421086164E-4	0.0	0.0	0.022684711469776242	9.653068710543082E-4
30-31	1.9306137421086164E-4	0.0	0.0	0.04430758538139275	9.653068710543082E-4
32-33	1.9306137421086164E-4	0.0	0.0	0.08166496129119447	9.653068710543082E-4
34-35	1.9306137421086164E-4	0.0	0.0	0.13050948896654246	9.653068710543082E-4
36-37	1.9306137421086164E-4	0.0	0.0	0.1907446377203313	9.653068710543082E-4
38-39	1.9306137421086164E-4	0.0	0.0	0.2634322451107207	9.653068710543082E-4
40-41	1.9306137421086164E-4	0.0	0.0	0.3436492460953337	9.653068710543082E-4
42-43	1.9306137421086164E-4	0.0	0.0	0.43004421105469426	9.653068710543082E-4
44-45	1.9306137421086164E-4	0.0	0.0	0.5374828658030388	9.653068710543082E-4
46-47	1.9306137421086164E-4	0.0	0.0	0.664131127285364	9.653068710543082E-4
48-49	1.9306137421086164E-4	0.0	0.0	0.7756240708921366	0.0011583682452651698
50-51	1.9306137421086164E-4	0.0	0.0	0.8704172056296697	0.0011583682452651698
52-53	1.9306137421086164E-4	0.0	0.0	0.9687819757901037	0.0013514296194760316
54-55	1.9306137421086164E-4	0.0	0.0	1.0910863563526845	0.0015444909936868931
56-57	1.9306137421086164E-4	0.0	0.0	1.222271560128965	0.0015444909936868931
58-59	1.9306137421086164E-4	0.0	0.0	1.3691912659034307	0.0016410216807923239
60-61	1.9306137421086164E-4	0.0	0.0	1.4982527945633919	0.0017375523678977546
62-63	1.9306137421086164E-4	0.0	0.0	1.630306774523621	0.0017375523678977546
64-65	1.9306137421086164E-4	0.0	0.0	1.770083209452285	0.0017375523678977546
66-67	1.9306137421086164E-4	0.0	0.0	1.9398806880707378	0.0017375523678977546
68-69	1.9306137421086164E-4	0.0	0.0	2.1088093905052414	0.0017375523678977546
70-71	1.9306137421086164E-4	0.0	0.0	2.268181554916308	0.0017375523678977546
72-73	1.9306137421086164E-4	0.0	0.0	2.418093711991042	0.0019306137421086164
74-75	1.9306137421086164E-4	0.0	0.0	2.58904955885476	0.0019306137421086164
76-77	1.9306137421086164E-4	0.0	0.0	2.79079869490511	0.0019306137421086164
78-79	1.9306137421086164E-4	0.0	0.0	2.9948645674459913	0.0019306137421086164
80-81	1.9306137421086164E-4	0.0	0.0	3.1956483966252875	0.0019306137421086164
82-83	1.9306137421086164E-4	0.0	0.0	3.3909299766395735	0.002123675116319478
84-85	1.9306137421086164E-4	0.0	0.0	3.5854393111570166	0.002123675116319478
86-87	1.9306137421086164E-4	0.0	0.0	3.8041778481379227	0.002123675116319478
88-89	1.9306137421086164E-4	0.0	0.0	4.050524161630983	0.002123675116319478
90-91	1.9306137421086164E-4	0.0	0.0	4.297739251307991	0.002123675116319478
92-93	1.9306137421086164E-4	0.0	0.0	4.521014730582852	0.002123675116319478
94-95	1.9306137421086164E-4	0.0	0.0	4.739367144815336	0.002123675116319478
96-97	1.9306137421086164E-4	0.0	0.0	5.011969805201073	0.002123675116319478
98-99	1.9306137421086164E-4	0.0	0.0	5.317585960576867	0.002123675116319478
100-101	1.9306137421086164E-4	0.0	0.0	5.639515802073479	0.002123675116319478
102-103	1.9306137421086164E-4	0.0	0.0	5.951309921424021	0.0024132671776357704
104-105	1.9306137421086164E-4	0.0	0.0	6.2503619900766445	0.002509797864741201
106-107	1.9306137421086164E-4	0.0	0.0	6.570843871266676	0.002509797864741201
108-109	1.9306137421086164E-4	0.0	0.0	6.922408633704655	0.002509797864741201
110-111	1.9306137421086164E-4	0.0	0.0	7.314902407475337	0.002509797864741201
112-113	1.9306137421086164E-4	0.0	0.0	7.684518408402031	0.002509797864741201
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1775	0.0	32.867607	1
GTATCAA	2220	0.0	25.998552	2
GTAAGTA	245	1.6159902E-8	24.286465	2
TATAGTG	130	0.0020514277	22.88311	5
AACGCAG	2565	0.0	22.49297	7
CAACGCA	2525	0.0	22.378174	6
TCAACGC	2590	0.0	22.282318	5
TATCAAC	2625	0.0	22.214018	3
ATCAACG	2635	0.0	21.903902	4
GTATACG	165	3.2008192E-4	21.647497	1
ATGGGGG	1170	0.0	21.357569	5
CCTATGC	145	0.0038725173	20.509943	8
ACGCAGA	2805	0.0	20.356392	8
CATGGGG	1915	0.0	20.196445	4
CGCAGAG	2905	0.0	19.860403	9
TGGGGGG	720	0.0	19.826279	6
ATACCGT	305	1.6469494E-7	19.501257	6
TACCGTC	215	8.5739186E-5	19.365202	7
ATATACG	185	6.964892E-4	19.290434	6
GTCTACA	345	2.8612703E-8	18.980778	1
>>END_MODULE