FastQCFastQC Report
Sun 1 Jan 2017
ERR1142928_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1142928_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences517970
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23060.44519952893024695No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19900.38419213467961466No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8980.17336911404135374No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCAACG1301.8189894E-1145.7999151
GGTATCA19250.038.3529741
GTATCAA27450.037.0904271
GTACTAG953.2709583E-431.3367861
GCGTAAG953.2709583E-431.3367861
ATCAACG32550.031.2578873
TCAACGC33350.031.04334
CAACGCA33000.031.0119445
AACGCAG34100.030.1860476
TATCAAC34200.029.749832
TATAACG850.0072069727.9998422
GCGCAGA2803.6379788E-1227.6435241
ACGCAGA38150.027.6053477
CGCAGAG40150.026.2302368
GCAGAGT42450.024.8090469
GAGTACT17150.023.76517112-13
GTGTACA3800.023.502591
GTACTTT18450.022.0906614-15
CAGAGTA41700.021.47409810-11
TTAGTAT1400.003153452421.249884
Produced by FastQC (version 0.11.5)