##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1142928_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	517970
Sequences flagged as poor quality	0
Sequence length	125
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	23.733633222001274	27.0	14.0	33.0	14.0	33.0
2	25.448330984419947	27.0	27.0	33.0	14.0	33.0
3	24.761439851728866	27.0	14.0	33.0	14.0	33.0
4	30.042956155761917	33.0	27.0	33.0	27.0	33.0
5	32.11416491302585	33.0	33.0	33.0	33.0	33.0
6	35.67818406471417	37.0	37.0	37.0	33.0	37.0
7	35.93468347587698	37.0	37.0	37.0	33.0	37.0
8	36.18116493233199	37.0	37.0	37.0	37.0	37.0
9	36.31399888024403	37.0	37.0	37.0	37.0	37.0
10-11	36.29506535127517	37.0	37.0	37.0	37.0	37.0
12-13	36.347814545243935	37.0	37.0	37.0	37.0	37.0
14-15	36.32184296387822	37.0	37.0	37.0	37.0	37.0
16-17	36.35055794737147	37.0	37.0	37.0	37.0	37.0
18-19	36.301597582871594	37.0	37.0	37.0	37.0	37.0
20-21	36.32288452999208	37.0	37.0	37.0	37.0	37.0
22-23	36.330303878603004	37.0	37.0	37.0	37.0	37.0
24-25	36.31631658204143	37.0	37.0	37.0	37.0	37.0
26-27	36.29253045543178	37.0	37.0	37.0	37.0	37.0
28-29	36.26793057512983	37.0	37.0	37.0	37.0	37.0
30-31	36.261630982489336	37.0	37.0	37.0	37.0	37.0
32-33	36.22517423789022	37.0	37.0	37.0	37.0	37.0
34-35	36.18888159545919	37.0	37.0	37.0	37.0	37.0
36-37	36.14549684344654	37.0	37.0	37.0	37.0	37.0
38-39	36.09927022800548	37.0	37.0	37.0	37.0	37.0
40-41	36.06482421761878	37.0	37.0	37.0	37.0	37.0
42-43	35.99165781802034	37.0	37.0	37.0	37.0	37.0
44-45	35.93715852269436	37.0	37.0	37.0	37.0	37.0
46-47	35.853955827557584	37.0	37.0	37.0	37.0	37.0
48-49	35.761753576461956	37.0	37.0	37.0	35.0	37.0
50-51	35.69599108056451	37.0	37.0	37.0	33.0	37.0
52-53	35.65292874104678	37.0	37.0	37.0	33.0	37.0
54-55	35.60325598007607	37.0	37.0	37.0	33.0	37.0
56-57	35.60091800683438	37.0	37.0	37.0	33.0	37.0
58-59	35.568170936540724	37.0	37.0	37.0	33.0	37.0
60-61	35.5370504083248	37.0	37.0	37.0	33.0	37.0
62-63	35.52583836901751	37.0	37.0	37.0	33.0	37.0
64-65	35.5063797131108	37.0	37.0	37.0	33.0	37.0
66-67	35.49857327644458	37.0	37.0	37.0	33.0	37.0
68-69	35.47152634322451	37.0	37.0	37.0	33.0	37.0
70-71	35.45088132517327	37.0	37.0	37.0	33.0	37.0
72-73	35.44974033245169	37.0	37.0	37.0	33.0	37.0
74-75	35.39125238913451	37.0	37.0	37.0	33.0	37.0
76-77	35.37215475799756	37.0	37.0	37.0	33.0	37.0
78-79	35.339566963337646	37.0	37.0	37.0	33.0	37.0
80-81	35.29293588431763	37.0	37.0	37.0	33.0	37.0
82-83	35.27394057570902	37.0	37.0	37.0	33.0	37.0
84-85	35.19844199471012	37.0	37.0	37.0	33.0	37.0
86-87	35.15678224607603	37.0	37.0	37.0	33.0	37.0
88-89	35.15941174199278	37.0	37.0	37.0	33.0	37.0
90-91	35.083390929976645	37.0	37.0	37.0	33.0	37.0
92-93	35.03468251057011	37.0	37.0	37.0	33.0	37.0
94-95	35.006341100835954	37.0	37.0	37.0	33.0	37.0
96-97	34.91335791648165	37.0	37.0	37.0	30.0	37.0
98-99	34.848066490337274	37.0	37.0	37.0	27.0	37.0
100-101	34.774100816649614	37.0	37.0	37.0	27.0	37.0
102-103	34.723741722493585	37.0	37.0	37.0	27.0	37.0
104-105	34.635204741587344	37.0	37.0	37.0	27.0	37.0
106-107	34.56742668494314	37.0	37.0	37.0	27.0	37.0
108-109	34.51951753962585	37.0	37.0	37.0	27.0	37.0
110-111	34.45775431009518	37.0	37.0	37.0	27.0	37.0
112-113	34.390446357897176	37.0	37.0	37.0	27.0	37.0
114-115	34.2976620267583	37.0	37.0	37.0	27.0	37.0
116-117	34.19641581558777	37.0	37.0	37.0	24.5	37.0
118-119	34.05938760932101	37.0	37.0	37.0	22.0	37.0
120-121	33.92473598857077	37.0	37.0	37.0	22.0	37.0
122-123	33.83775315172693	37.0	35.0	37.0	22.0	37.0
124-125	32.53865957487885	37.0	30.0	37.0	18.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	44.0
16	59.0
17	16.0
18	55.0
19	195.0
20	641.0
21	1401.0
22	2452.0
23	3587.0
24	3861.0
25	3548.0
26	3492.0
27	3793.0
28	4721.0
29	5845.0
30	7508.0
31	10287.0
32	14320.0
33	21609.0
34	36594.0
35	90247.0
36	303695.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	52.498985957931744	13.361984045738126	12.873312344272112	21.265717652058022
2	18.15466652251306	19.513829093029273	35.934018835213124	26.397485549244543
3	21.712068266501923	23.682645713072187	28.5477923431859	26.057493677239997
4	15.552728262611323	17.175611573710295	34.95743937355083	32.31422079012756
5	16.216659748433745	34.93855643830062	31.625334247183634	17.219449566081995
6	31.287526304612236	33.218912292217695	18.21688514778848	17.276676255381584
7	27.51665154352569	31.55819835125586	21.531748942988976	19.393401162229473
8	26.4503735737591	32.19993435913277	20.6008069965442	20.748885070563933
9	26.875301658397206	16.57567040562195	20.92746684170898	35.62156109427187
10-11	26.19640133598471	24.94526710041122	26.05170183601367	22.8066297275904
12-13	26.680888854566863	23.38986813908141	26.39120026256347	23.53804274378825
14-15	24.98129708910308	24.190996539358164	24.70541105378233	26.12229531775642
16-17	24.05226171399888	26.58088306272564	25.67812807691565	23.688727146359827
18-19	23.924358553584185	25.2452844759349	27.81975790103674	23.010599069444176
20-21	24.994256368673557	24.185128338787372	27.6967555722877	23.123859720251367
22-23	24.84159314245999	23.937679788404733	27.508832557870143	23.71189451126513
24-25	24.112303801378456	24.57150027993899	27.57109485105315	23.7451010676294
26-27	23.863701083761534	24.76067375273788	27.634229758989274	23.741395404511312
28-29	23.87140493506343	24.788355242708512	27.846368591049238	23.49387123117882
30-31	24.520591980214554	24.335248230305055	27.792391832132623	23.351767957347768
32-33	24.121474216653475	24.38394115489314	27.591848948780818	23.902735679672567
34-35	24.161824043863543	24.689557310268935	27.56858505318841	23.58003359267911
36-37	24.200193447870433	24.650606503478006	27.481953553204924	23.66724649544664
38-39	24.094445624263955	24.620827461049867	27.562310558526555	23.722416356159624
40-41	24.042848124115533	24.52347534311899	27.611594516274142	23.822082016491333
42-43	24.403459303756716	24.459737183268	27.290524175837966	23.846279337137318
44-45	23.98256655790876	24.691584454698148	27.26499604224183	24.060852945151264
46-47	23.928339409945952	24.6036687488356	27.306627127659063	24.16136471355939
48-49	24.0501111554081	24.902479590940377	27.16854403308309	23.878865220568432
50-51	23.838225683608172	25.003354451091138	27.203295181077614	23.955124684223076
52-53	24.094681707521907	24.74877743176689	26.879608160365432	24.276932700345775
54-55	23.998540453193147	24.58496550951891	27.08434288538503	24.332151151902913
56-57	23.930509287726956	24.674376916743007	26.70654682668927	24.68856696884077
58-59	23.79153232812711	24.873737861266097	27.01652605363245	24.31820375697434
60-61	24.10091318030002	24.68395853041682	27.322238739695347	23.892889549587814
62-63	23.840376855802457	24.69998262447632	27.21267640983068	24.246964109890534
64-65	23.818078267081106	24.38963646543236	27.261038284070505	24.53124698341603
66-67	23.922910593277603	24.43548854180744	27.388844913798096	24.25275595111686
68-69	23.737378612660965	24.520821669208644	27.42002432573315	24.321775392397242
70-71	23.55503214471881	24.57429966986505	27.629978570187465	24.240689615228682
72-73	23.80832866768346	24.707898140818966	27.488271521516687	23.995501669980886
74-75	23.863544220707762	24.45894549877406	27.524084406432802	24.15342587408537
76-77	23.775797826128926	24.37274359518891	27.495800915110912	24.355657663571247
78-79	23.690754290789044	24.59775662683167	27.553526265999963	24.157962816379328
80-81	23.483792497634997	24.73502326389559	27.785875629862733	23.995308608606674
82-83	23.662084676718727	24.6942873139371	27.592331602216348	24.051296407127825
84-85	23.649149564646603	24.734926733208486	27.26750584010657	24.34841786203834
86-87	23.382724868235613	24.918914222831436	27.351680599262508	24.346680309670443
88-89	23.379346294186924	24.481051026121204	27.55796667760681	24.581636002085062
90-91	23.71932737417225	24.491765932389907	27.49261540243643	24.29629129100141
92-93	23.59316176612545	24.72305345869452	27.408054520532076	24.275730254647954
94-95	23.304148888931792	24.767747166824332	27.33034731741221	24.59775662683167
96-97	23.49180454466475	24.628936038766724	27.15890881711296	24.720350599455568
98-99	23.25684885225013	24.699403440353688	27.117786744406047	24.925960962990136
100-101	23.3736509836477	25.0	26.955615190068922	24.670733826283374
102-103	23.439195320192287	24.7973820877657	27.310944649304012	24.452477942737996
104-105	23.532250902561923	24.77991003339962	27.067880379172536	24.61995868486592
106-107	23.639110373187634	24.825858640461803	27.01652605363245	24.51850493271811
108-109	23.55454949128328	24.862443770874762	26.90638453964515	24.676622198196807
110-111	23.175830190445946	25.29912533291117	26.907620830224232	24.617423646418654
112-113	23.241693534374576	25.278297970924957	26.88350676680117	24.5965017278993
114-115	23.11427302739541	25.366527018939323	26.839778365542404	24.679421588122864
116-117	23.243720678803793	25.294418595671566	26.660810471648936	24.801050253875704
118-119	23.09182614571518	25.145449718850305	26.859376885392283	24.90334725004223
120-121	23.082722589912503	25.14209920687019	27.104458362615553	24.67071984060176
122-123	22.847627138278565	25.405935050391935	26.98372398347299	24.76271382785651
124-125	22.752831494579304	25.448169145421005	26.988127656411535	24.810871703588152
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	251.0
1	207.5
2	315.0
3	484.5
4	373.5
5	292.0
6	425.5
7	804.5
8	1486.0
9	1833.5
10	1514.5
11	1113.5
12	921.0
13	784.0
14	681.0
15	669.0
16	650.0
17	609.5
18	559.5
19	519.5
20	513.0
21	495.5
22	516.5
23	558.0
24	609.5
25	709.0
26	850.5
27	1031.0
28	1324.0
29	1814.5
30	2378.5
31	2978.0
32	3724.0
33	4518.5
34	5298.0
35	6136.0
36	7151.5
37	8225.0
38	9330.0
39	10190.5
40	10783.0
41	11341.0
42	12043.0
43	13418.5
44	15085.0
45	16301.5
46	17769.0
47	19477.0
48	21243.0
49	22882.0
50	24085.0
51	24197.0
52	23371.0
53	23085.5
54	22586.5
55	21629.0
56	20404.5
57	18604.0
58	16867.0
59	14878.0
60	12761.0
61	10688.5
62	8724.0
63	6846.5
64	5102.5
65	4043.5
66	3146.5
67	2417.0
68	1985.5
69	1692.5
70	1434.0
71	1185.0
72	1033.5
73	1024.5
74	830.5
75	671.5
76	570.0
77	367.5
78	255.0
79	164.0
80	100.0
81	64.0
82	37.5
83	22.0
84	13.5
85	5.5
86	5.5
87	4.5
88	2.0
89	1.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04633472981060679
2	7.722454968434464E-4
3	0.0
4	5.791841226325849E-4
5	0.0028959206131629243
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	4.8265343552715404E-4
16-17	0.0
18-19	0.0
20-21	9.653068710543081E-4
22-23	0.0
24-25	0.0
26-27	0.001061837558159739
28-29	5.791841226325849E-4
30-31	0.002606328551846632
32-33	0.0
34-35	0.0
36-37	1.930613742108616E-4
38-39	0.0
40-41	1.930613742108616E-4
42-43	8.687761839488774E-4
44-45	0.0
46-47	9.65306871054308E-5
48-49	2.8959206131629246E-4
50-51	2.8959206131629246E-4
52-53	5.791841226325849E-4
54-55	1.930613742108616E-4
56-57	2.8959206131629246E-4
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.0
102-103	0.0
104-105	0.0
106-107	0.0
108-109	0.0
110-111	2.8959206131629246E-4
112-113	0.0
114-115	0.0
116-117	0.0
118-119	0.0014479603065814621
120-121	0.004247350232638956
122-123	0.0057918412263258485
124-125	2.8959206131629246E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	517970.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	81.01974651644497
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.64748110482704	72.63216194950877
2	7.034072535963659	11.397975476841257
3	1.6513126892793921	4.013668065144163
4	0.6426437213023406	2.0826732560120216
5	0.3298335959527776	1.3361517168350787
6	0.193573944854587	0.9409987166584162
7	0.12213472587369453	0.6926727171799558
8	0.0782882382129254	0.5074314572184222
9	0.055610696964074745	0.4055008114468954
>10	0.22427585361616026	2.9990998057274187
>50	0.013848596095107347	0.7694397360530305
>100	0.006125339705361853	1.1015113328932358
>500	2.6631911762441774E-4	0.1937624244736743
>1k	5.326382352488355E-4	0.9269525340076891
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2306	0.44519952893024695	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1990	0.38419213467961466	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	898	0.17336911404135374	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	1.9306137421086164E-4	0.0
6	0.0	0.0	0.0	1.9306137421086164E-4	0.0
7	0.0	0.0	0.0	1.9306137421086164E-4	0.0
8	0.0	0.0	0.0	1.9306137421086164E-4	0.0
9	0.0	0.0	0.0	1.9306137421086164E-4	0.0
10-11	0.0	0.0	0.0	1.9306137421086164E-4	0.0
12-13	0.0	0.0	0.0	4.826534355271541E-4	1.9306137421086164E-4
14-15	0.0	0.0	0.0	5.791841226325849E-4	1.9306137421086164E-4
16-17	0.0	0.0	0.0	5.791841226325849E-4	1.9306137421086164E-4
18-19	0.0	0.0	0.0	5.791841226325849E-4	1.9306137421086164E-4
20-21	0.0	0.0	0.0	9.653068710543081E-4	1.9306137421086164E-4
22-23	0.0	0.0	0.0	0.0019306137421086164	2.8959206131629246E-4
24-25	0.0	0.0	0.0	0.005019595729482403	5.791841226325849E-4
26-27	0.0	0.0	0.0	0.011294090391335405	5.791841226325849E-4
28-29	0.0	0.0	0.0	0.02249165009556538	5.791841226325849E-4
30-31	0.0	0.0	0.0	0.04411452400718188	5.791841226325849E-4
32-33	0.0	0.0	0.0	0.08137536922987818	5.791841226325849E-4
34-35	0.0	0.0	0.0	0.12983377415680444	7.722454968434466E-4
36-37	0.0	0.0	0.0	0.18987586153638242	9.653068710543082E-4
38-39	0.0	0.0	0.0	0.26275653030098267	9.653068710543082E-4
40-41	0.0	0.0	0.0	0.3429735312855957	9.653068710543082E-4
42-43	0.0	0.0	0.0	0.42985114968048344	9.653068710543082E-4
44-45	0.0	0.0	0.0	0.5369036816804063	0.0011583682452651698
46-47	0.0	0.0	0.0	0.6636484738498369	0.0011583682452651698
48-49	0.0	0.0	0.0	0.7755275402050312	0.0011583682452651698
50-51	0.0	0.0	0.0	0.8708033283780914	0.0011583682452651698
52-53	0.0	0.0	0.0	0.9703264667837905	0.0011583682452651698
54-55	0.0	0.0	0.0	1.0931135007818984	0.0011583682452651698
56-57	0.0	0.0	0.0	1.2248778886808118	0.0011583682452651698
58-59	0.0	0.0	0.0	1.372859432013437	0.0011583682452651698
60-61	0.0	0.0	0.0	1.503272390292874	0.0011583682452651698
62-63	0.0	0.0	0.0	1.6360020850628416	0.0011583682452651698
64-65	0.0	0.0	0.0	1.7760681120528217	0.0011583682452651698
66-67	0.0	0.0	0.0	1.947796204413383	0.0011583682452651698
68-69	0.0	0.0	0.0	2.117400621657625	0.0011583682452651698
70-71	0.0	0.0	0.0	2.277158908817113	0.0015444909936868931
72-73	0.0	0.0	0.0	2.42890514894685	0.0015444909936868931
74-75	0.0	0.0	0.0	2.6003436492460956	0.0015444909936868931
76-77	0.0	0.0	0.0	2.80411992972566	0.0015444909936868931
78-79	0.0	0.0	0.0	3.0119504990636523	0.0015444909936868931
80-81	0.0	0.0	0.0	3.2164990250400605	0.0015444909936868931
82-83	0.0	0.0	0.0	3.4148695870417205	0.0015444909936868931
84-85	0.0	0.0	0.0	3.6134332104175915	0.0015444909936868931
86-87	0.0	0.0	0.0	3.8349711373245556	0.0015444909936868931
88-89	0.0	0.0	0.0	4.085661331737359	0.0015444909936868931
90-91	0.0	0.0	0.0	4.334613973782265	0.0015444909936868931
92-93	0.0	0.0	0.0	4.559820066799236	0.0015444909936868931
94-95	0.0	0.0	0.0	4.781261463019094	0.0020271444292140474
96-97	0.0	0.0	0.0	5.057725350889047	0.002123675116319478
98-99	0.0	0.0	0.0	5.3678784485587965	0.002123675116319478
100-101	0.0	0.0	0.0	5.6927042106685715	0.002123675116319478
102-103	0.0	0.0	0.0	6.006525474448328	0.002123675116319478
104-105	0.0	0.0	0.0	6.309728362646485	0.002123675116319478
106-107	0.0	0.0	0.0	6.637256983995211	0.0023167364905303397
108-109	0.0	0.0	0.0	6.995289302469255	0.002606328551846632
110-111	0.0	0.0	0.0	7.394250632276	0.0027028592389520627
112-113	0.0	0.0	0.0	7.771492557484024	0.0027028592389520627
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCAACG	130	1.8189894E-11	45.799915	1
GGTATCA	1925	0.0	38.352974	1
GTATCAA	2745	0.0	37.090427	1
GTACTAG	95	3.2709583E-4	31.336786	1
GCGTAAG	95	3.2709583E-4	31.336786	1
ATCAACG	3255	0.0	31.257887	3
TCAACGC	3335	0.0	31.0433	4
CAACGCA	3300	0.0	31.011944	5
AACGCAG	3410	0.0	30.186047	6
TATCAAC	3420	0.0	29.74983	2
TATAACG	85	0.00720697	27.999842	2
GCGCAGA	280	3.6379788E-12	27.643524	1
ACGCAGA	3815	0.0	27.605347	7
CGCAGAG	4015	0.0	26.230236	8
GCAGAGT	4245	0.0	24.809046	9
GAGTACT	1715	0.0	23.765171	12-13
GTGTACA	380	0.0	23.50259	1
GTACTTT	1845	0.0	22.09066	14-15
CAGAGTA	4170	0.0	21.474098	10-11
TTAGTAT	140	0.0031534524	21.24988	4
>>END_MODULE