FastQCFastQC Report
Sun 1 Jan 2017
ERR1142926_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1142926_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences285759
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12270.4293828015915509No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA4740.16587404071262846No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT3530.12353066745054399No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGTTCG150.004230547759.5174496-97
ACGGCGT300.001310583839.65049736-37
TTAGACT750.00388663431.76494
GTCTTAT1055.8185094E-428.3963781
ATTAGAA1055.8604876E-428.3615172
GGTATCA19050.028.1727871
CATGGGT3750.025.4119224
GTACAAG1651.0871139E-525.2985921
GTATCAA24050.025.0431251
ATGGGAG6500.022.9073815
CATGGGG14050.022.4671674
ATGGGGA4800.022.3346965
TACAAGG1653.1856043E-421.6578882
GTACATG40550.020.735311
ACATGGG39700.020.4031473
TACATGG40950.020.3621162
ATCAACG28900.020.196543
TATCAAC29550.020.1553942
GAAAAAA19850.020.127811
CTTATCA750.00470699819.83045856-57
Produced by FastQC (version 0.11.5)