FastQCFastQC Report
Sun 1 Jan 2017
ERR1142926_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1142926_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences285759
Sequences flagged as poor quality0
Sequence length125
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23040.8062738181474599No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21870.7653302258196593No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12630.441980830000105No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6260.2190657162154123No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA4830.169023547814767No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT4200.14697699809979736No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3900.136478641092669No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG2960.10358378913700006No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20750.046.7881931
CTCAACG2700.046.3257331
CCGCAGA2800.042.544041
GTATCAA31450.040.1496731
GTGTAAT758.123453E-539.707771
ATCAACG38300.033.872273
TATCAAC38600.033.6090132
CTAACTC902.3862693E-433.0608524
CAACGCA40950.031.9709385
TCAACGC41500.031.6906224
AACGCAG42250.031.404276
TGTAAGT1403.0588963E-629.7808281
GTACACT1403.084051E-629.7495636
ACGCAGA45150.029.2553837
GAGTACT21100.028.3396312-13
CGCAGAG46950.028.1337728
AGCACCG850.00719581428.0044885
GCAGAGT48200.027.404169
GTACTTT23550.025.13869714-15
CATGGGT4500.023.8038164
Produced by FastQC (version 0.11.5)