##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1142926_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	285759
Sequences flagged as poor quality	0
Sequence length	125
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	22.075766642520446	14.0	14.0	33.0	14.0	33.0
2	27.236783443391108	27.0	27.0	33.0	14.0	33.0
3	31.041916440077127	33.0	33.0	33.0	27.0	33.0
4	30.82043260229774	33.0	33.0	33.0	27.0	33.0
5	32.124384533820454	33.0	33.0	33.0	33.0	33.0
6	35.291773837394445	37.0	37.0	37.0	33.0	37.0
7	35.502857302832105	37.0	37.0	37.0	33.0	37.0
8	35.775804086660436	37.0	37.0	37.0	33.0	37.0
9	36.07044397551783	37.0	37.0	37.0	37.0	37.0
10-11	36.10810858100707	37.0	37.0	37.0	37.0	37.0
12-13	36.18286038235016	37.0	37.0	37.0	37.0	37.0
14-15	36.16807519623179	37.0	37.0	37.0	37.0	37.0
16-17	36.17916321095748	37.0	37.0	37.0	37.0	37.0
18-19	36.178612047214614	37.0	37.0	37.0	37.0	37.0
20-21	36.09938794578649	37.0	37.0	37.0	37.0	37.0
22-23	36.13792741435965	37.0	37.0	37.0	37.0	37.0
24-25	36.15927407360748	37.0	37.0	37.0	37.0	37.0
26-27	36.094682932121124	37.0	37.0	37.0	37.0	37.0
28-29	36.079815508872855	37.0	37.0	37.0	37.0	37.0
30-31	36.0236125546352	37.0	37.0	37.0	37.0	37.0
32-33	35.937412294975836	37.0	37.0	37.0	37.0	37.0
34-35	35.84260163284446	37.0	37.0	37.0	33.0	37.0
36-37	35.74872532448671	37.0	37.0	37.0	33.0	37.0
38-39	35.66469822472783	37.0	37.0	37.0	33.0	37.0
40-41	35.5622972504803	37.0	37.0	37.0	33.0	37.0
42-43	35.44612243183941	37.0	37.0	37.0	33.0	37.0
44-45	35.27979346232314	37.0	37.0	37.0	33.0	37.0
46-47	35.070412480446805	37.0	37.0	37.0	33.0	37.0
48-49	34.77045692349148	37.0	37.0	37.0	27.0	37.0
50-51	34.65308879160412	37.0	37.0	37.0	27.0	37.0
52-53	34.591235971570455	37.0	37.0	37.0	27.0	37.0
54-55	34.505779345532424	37.0	37.0	37.0	27.0	37.0
56-57	34.471888899387245	37.0	37.0	37.0	27.0	37.0
58-59	34.41918714721146	37.0	37.0	37.0	27.0	37.0
60-61	34.38333350830595	37.0	37.0	37.0	27.0	37.0
62-63	34.374290923470475	37.0	37.0	37.0	27.0	37.0
64-65	34.350330173327876	37.0	37.0	37.0	27.0	37.0
66-67	34.33434642478452	37.0	37.0	37.0	27.0	37.0
68-69	34.26178528060359	37.0	37.0	37.0	27.0	37.0
70-71	34.24978915799677	37.0	37.0	37.0	27.0	37.0
72-73	34.249098016160474	37.0	37.0	37.0	27.0	37.0
74-75	34.20625247148821	37.0	37.0	37.0	27.0	37.0
76-77	34.15418412018519	37.0	37.0	37.0	27.0	37.0
78-79	34.06588068967207	37.0	37.0	37.0	27.0	37.0
80-81	33.977635000122476	37.0	37.0	37.0	27.0	37.0
82-83	33.97788521096449	37.0	37.0	37.0	24.5	37.0
84-85	33.92888587935988	37.0	37.0	37.0	22.0	37.0
86-87	33.86142518695824	37.0	37.0	37.0	22.0	37.0
88-89	33.7830077092935	37.0	37.0	37.0	22.0	37.0
90-91	33.69528343814193	37.0	37.0	37.0	22.0	37.0
92-93	33.583097995163754	37.0	33.0	37.0	18.0	37.0
94-95	33.537232423125786	37.0	33.0	37.0	14.0	37.0
96-97	33.47358263431773	37.0	33.0	37.0	14.0	37.0
98-99	33.365335824943394	37.0	33.0	37.0	14.0	37.0
100-101	33.29183682753649	37.0	33.0	37.0	14.0	37.0
102-103	33.217263498262525	37.0	33.0	37.0	14.0	37.0
104-105	33.061973551139246	37.0	33.0	37.0	14.0	37.0
106-107	32.973927330372796	37.0	33.0	37.0	14.0	37.0
108-109	32.88786704880686	37.0	33.0	37.0	14.0	37.0
110-111	32.83573220790946	37.0	33.0	37.0	14.0	37.0
112-113	32.77063714528676	37.0	33.0	37.0	14.0	37.0
114-115	32.62367414499631	37.0	33.0	37.0	14.0	37.0
116-117	32.5732540357434	37.0	33.0	37.0	14.0	37.0
118-119	32.40877277706039	37.0	33.0	37.0	14.0	37.0
120-121	32.28256502857303	37.0	33.0	37.0	14.0	37.0
122-123	32.19180848197257	37.0	33.0	37.0	14.0	37.0
124-125	30.597634720166297	35.0	27.5	37.0	14.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	16.0
16	19.0
17	9.0
18	50.0
19	331.0
20	1309.0
21	2752.0
22	4236.0
23	5829.0
24	4454.0
25	3078.0
26	2595.0
27	2635.0
28	3223.0
29	3911.0
30	5256.0
31	7146.0
32	10263.0
33	16145.0
34	27024.0
35	58929.0
36	126549.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.487046632124354	14.117070438313961	13.126312841338747	30.269570088222935
2	16.611724688195523	20.768067862092135	39.35105474600708	23.26915270370526
3	19.453173664429343	28.05660733907712	29.36575703917301	23.12446195732053
4	13.56295210131969	19.931478325383466	33.99032017609861	32.51524939719824
5	15.181239807666735	35.714635665642476	32.303659791289014	16.80046473540178
6	27.846891961408037	35.2958262031992	19.293530562466973	17.56375127292579
7	24.720831189918776	33.61398941065723	23.11598234876242	18.549197050661572
8	26.061471379729074	31.29805185488471	21.224878306545026	21.415598458841192
9	27.037818581392013	16.09223156575996	21.609818063473067	35.26013178937497
10-11	24.89702861502175	25.95841250844243	26.29313512435304	22.851423752182782
12-13	26.925116052009496	25.225761183949214	26.779187102260497	21.06993566178079
14-15	24.898078450722462	24.775422646355846	26.75471288743312	23.571786015488577
16-17	22.25214253969254	26.84342400414335	27.773403462358143	23.131029993805967
18-19	22.08560725227771	24.755694056344783	31.319278341676625	21.839420349700884
20-21	24.45171563052265	23.14307711151161	31.1928050252839	21.212402232681843
22-23	23.876938259162444	23.477125829807637	30.977851966167297	21.66808394486263
24-25	22.556713297113813	24.37031399000901	31.353332808412727	21.71963990446445
26-27	22.375868965360368	24.356226367554193	31.26665535167179	22.00124931541365
28-29	21.78234106362354	24.14622111639528	32.00809073380016	22.06334708618101
30-31	22.411177505008705	23.824988407800458	31.79867192762968	21.965162159561157
32-33	21.90184736088802	24.08375589220287	31.498920418954434	22.515476327954676
34-35	22.140335037566622	24.163543405457048	31.62052638762034	22.075595169355996
36-37	22.161506724197665	24.285324346739735	31.046616204563986	22.506552724498615
38-39	22.407343250781253	23.87851301271351	31.164547748277393	22.549595988227843
40-41	22.015947143809502	24.158505580800437	31.37566424560771	22.44988302978235
42-43	22.52373945131991	23.96481644465519	30.887620140783213	22.623823963241687
44-45	22.41836652563874	24.14324658190993	30.467631815620855	22.970755076830475
46-47	22.34330327303777	24.27745057898439	30.26098915519721	23.11825699278063
48-49	22.5572947833664	24.530635955472967	29.823382640616742	23.088686620543886
50-51	22.429739745729794	24.792919908034392	29.362504768003806	23.414835578232008
52-53	22.520025615991095	24.773847892804778	29.034956029381405	23.671170461822726
54-55	22.549071070377487	24.78837061999797	28.954468625660084	23.70808968396446
56-57	22.591064498406	24.593801070132525	28.708807071693283	24.106327359768194
58-59	22.574267127194595	24.502465364170508	29.051053510125662	23.872213998509235
60-61	22.685899656703725	24.62722083993855	29.223926455509712	23.46295304784801
62-63	22.267618054437673	24.621357932236368	29.29174336326542	23.81928065006054
64-65	22.14733394223804	24.439475222127736	29.55759923571961	23.855591599914614
66-67	22.613286020737753	24.288823799075445	29.467488338075093	23.63040184211171
68-69	22.60138788279634	24.218309834510897	29.462764077421884	23.717538205270873
70-71	22.256866800345744	24.177541214799884	30.133434117560604	23.432157867293768
72-73	22.59771345784385	24.283049702721524	30.327303776958907	22.79193306247572
74-75	22.523875013560378	24.122949758362815	30.47148121319014	22.88169401488667
76-77	22.209624193813667	24.26310282440798	30.470256404872636	23.057016576905713
78-79	22.12843689962521	24.3929325060628	30.424938497125197	23.05369209718679
80-81	21.90272222397195	24.652416896755657	30.59571177110782	22.84914910816457
82-83	22.261241115765383	24.37945961457032	30.564216700086437	22.79508256957786
84-85	22.1947515213869	24.549008080235442	30.097739703736366	23.158500694641287
86-87	22.038151029363902	24.607973852092147	30.189950272782312	23.16392484576164
88-89	22.265615431185022	24.043862135575782	30.277611553791832	23.412910879447367
90-91	22.420816142273733	24.115600908457825	30.117511609433123	23.346071339835316
92-93	22.24251904576934	24.482343513240178	29.88357322079095	23.39156422019954
94-95	21.600544514783437	24.494941541648732	29.91576818227947	23.988745761288357
96-97	21.969561763584	24.4351009067081	29.57002229151138	24.025315038196524
98-99	21.82437267832803	24.62376447244789	29.271220278661875	24.280642570562204
100-101	22.15353550906711	24.855997032454034	28.885455525304625	24.105011933174225
102-103	22.37501246682076	24.888848450702252	28.89257887341934	23.84356020905765
104-105	22.346841826227394	25.008354957070395	28.60807290071861	24.0367303159836
106-107	22.25480606876086	25.07484466778766	28.56415469706063	24.10619456639085
108-109	22.292950165961816	25.280437852242365	28.1925122087357	24.23409977306012
110-111	22.077033579053644	25.564943824943093	28.088228346663353	24.269794249339917
112-113	22.146886526361467	25.570062780394597	27.968245858383668	24.314804834860265
114-115	22.15059972179208	25.71481063489147	27.71300840747837	24.42158123583808
116-117	22.11136696085499	25.827448400394736	27.71121018484172	24.34997445390855
118-119	21.92820835848578	25.533363078834327	28.05875611313789	24.479672449542004
120-121	21.910224421448994	25.44049664575146	28.222580724181732	24.42669820861781
122-123	21.769210983554068	25.83861321518996	28.138489507601804	24.253686293654166
124-125	21.821064124637804	25.65632917594031	28.113495429667267	24.409111269754614
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	109.0
1	115.0
2	229.5
3	308.5
4	245.0
5	254.0
6	315.0
7	578.0
8	1061.5
9	1286.0
10	1180.0
11	1026.5
12	940.5
13	908.5
14	915.0
15	904.5
16	893.5
17	873.5
18	839.5
19	877.0
20	870.0
21	854.0
22	880.0
23	889.5
24	901.5
25	955.5
26	1070.5
27	1218.0
28	1378.0
29	1688.5
30	2192.0
31	2645.0
32	3225.0
33	3941.5
34	4576.0
35	5222.5
36	5807.5
37	6365.0
38	6696.5
39	6833.5
40	6715.5
41	6543.0
42	6774.5
43	7187.5
44	7794.5
45	8410.5
46	9192.0
47	10092.0
48	10896.5
49	11612.5
50	12400.0
51	11959.0
52	10578.5
53	10179.0
54	9973.0
55	9686.0
56	9341.5
57	8403.5
58	7660.0
59	6966.5
60	6040.0
61	5030.0
62	3943.0
63	3072.0
64	2391.5
65	1988.5
66	1578.0
67	1178.0
68	958.0
69	773.0
70	642.5
71	580.0
72	541.0
73	541.5
74	438.0
75	350.0
76	269.0
77	218.0
78	203.5
79	146.0
80	110.0
81	102.5
82	92.5
83	68.5
84	46.0
85	33.0
86	24.0
87	13.0
88	5.0
89	1.0
90	0.0
91	0.0
92	0.0
93	0.5
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04164348279494259
2	0.0010498357007128383
3	3.499452335709461E-4
4	0.0034994523357094616
5	0.0017497261678547308
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0012248083174983115
14-15	0.0
16-17	0.0
18-19	1.7497261678547306E-4
20-21	0.0013997809342837844
22-23	0.0
24-25	5.249178503564191E-4
26-27	8.748630839273654E-4
28-29	0.0
30-31	0.00227464401821115
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	8.748630839273654E-4
42-43	1.7497261678547306E-4
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	3.499452335709461E-4
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	1.7497261678547306E-4
100-101	3.499452335709461E-4
102-103	1.7497261678547306E-4
104-105	1.7497261678547306E-4
106-107	1.7497261678547306E-4
108-109	1.7497261678547306E-4
110-111	1.7497261678547306E-4
112-113	3.499452335709461E-4
114-115	5.249178503564191E-4
116-117	3.499452335709461E-4
118-119	5.249178503564191E-4
120-121	6.998904671418922E-4
122-123	0.0015747535510692576
124-125	0.0010498357007128383
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	285759.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	69.27338867995547
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.77876545532102	59.42185759873191
2	9.587091600432586	13.282606454942059
3	1.975514095832277	4.105516674099604
4	0.7939550106480037	2.1999981618806936
5	0.3853606600454568	1.334761939264656
6	0.2587187812497232	1.075339601538987
7	0.1739394594688282	0.8434563047795848
8	0.13753303274330414	0.762190338684796
9	0.10652893879914913	0.6641658524786981
>10	0.7373660760108797	9.546804359983476
>50	0.04494625995631343	2.113437171793406
>100	0.01808812900680906	2.227348625537211
>500	5.481251214184565E-4	0.2376952334787409
>1k	0.0016443753642553694	2.1848216828061906
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2304	0.8062738181474599	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2187	0.7653302258196593	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1263	0.441980830000105	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	626	0.2190657162154123	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	483	0.169023547814767	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	420	0.14697699809979736	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	390	0.136478641092669	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG	296	0.10358378913700006	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	1.7497261678547308E-4	0.0
16-17	0.0	0.0	0.0	3.4994523357094616E-4	0.0
18-19	0.0	0.0	0.0	3.4994523357094616E-4	0.0
20-21	0.0	0.0	0.0	0.0012248083174983115	0.0
22-23	1.7497261678547308E-4	0.0	0.0	0.004199342802851354	0.0
24-25	3.4994523357094616E-4	0.0	0.0	0.009098576072844599	0.0
26-27	3.4994523357094616E-4	0.0	0.0	0.01942196046318751	0.0
28-29	3.4994523357094616E-4	0.0	0.0	0.03534446859066556	0.0
30-31	3.4994523357094616E-4	0.0	0.0	0.06246522419241389	0.0
32-33	3.4994523357094616E-4	0.0	0.0	0.10165909035235986	0.0
34-35	3.4994523357094616E-4	0.0	0.0	0.14522727193194265	0.0
36-37	3.4994523357094616E-4	0.0	0.0	0.20821741397471294	0.0
38-39	3.4994523357094616E-4	0.0	0.0	0.3139008745131387	0.0
40-41	3.4994523357094616E-4	0.0	0.0	0.41678477318299684	0.0
42-43	3.4994523357094616E-4	0.0	0.0	0.5219433158710662	0.0
44-45	3.4994523357094616E-4	0.0	0.0	0.6160785837016507	0.0
46-47	3.4994523357094616E-4	0.0	0.0	0.7327853190975613	0.0
48-49	3.4994523357094616E-4	0.0	0.0	0.8521166437452539	0.0
50-51	3.4994523357094616E-4	0.0	0.0	0.9651489541886695	0.0
52-53	3.4994523357094616E-4	0.0	0.0	1.0650583183731746	0.0
54-55	3.4994523357094616E-4	0.0	0.0	1.1686421075101747	0.0
56-57	3.4994523357094616E-4	0.0	0.0	1.2762502668332405	0.0
58-59	3.4994523357094616E-4	0.0	0.0	1.4157034424112624	0.0
60-61	3.4994523357094616E-4	0.0	0.0	1.5443083157485853	0.0
62-63	3.4994523357094616E-4	0.0	0.0	1.6597902428269977	0.0
64-65	3.4994523357094616E-4	0.0	0.0	1.776496978222908	0.0
66-67	3.4994523357094616E-4	0.0	0.0	1.8960032754873861	0.0
68-69	3.4994523357094616E-4	0.0	0.0	2.046479725922893	0.0
70-71	3.4994523357094616E-4	0.0	0.0	2.2013304917780365	0.0
72-73	3.4994523357094616E-4	0.0	0.0	2.350057216045689	0.0
74-75	3.4994523357094616E-4	0.0	0.0	2.489860336857282	1.7497261678547308E-4
76-77	3.4994523357094616E-4	0.0	0.0	2.641386622993502	3.4994523357094616E-4
78-79	3.4994523357094616E-4	0.0	0.0	2.824932898001463	3.4994523357094616E-4
80-81	3.4994523357094616E-4	0.0	0.0	3.013028461045846	3.4994523357094616E-4
82-83	3.4994523357094616E-4	0.0	0.0	3.2207209571702027	3.4994523357094616E-4
84-85	3.4994523357094616E-4	0.0	0.0	3.4131908356342233	3.4994523357094616E-4
86-87	3.4994523357094616E-4	0.0	0.0	3.5930626856896897	3.4994523357094616E-4
88-89	3.4994523357094616E-4	0.0	0.0	3.7935813045258415	3.4994523357094616E-4
90-91	3.4994523357094616E-4	0.0	0.0	4.024545158682666	3.4994523357094616E-4
92-93	3.4994523357094616E-4	0.0	0.0	4.253059396204494	3.4994523357094616E-4
94-95	3.4994523357094616E-4	0.0	0.0	4.486122921762744	3.4994523357094616E-4
96-97	3.4994523357094616E-4	0.0	0.0	4.71586196760207	3.4994523357094616E-4
98-99	3.4994523357094616E-4	0.0	0.0	4.955749425214954	3.4994523357094616E-4
100-101	3.4994523357094616E-4	0.0	0.0	5.2075350207692495	3.4994523357094616E-4
102-103	3.4994523357094616E-4	0.0	0.0	5.471043781648172	3.4994523357094616E-4
104-105	3.4994523357094616E-4	0.0	0.0	5.733327734209596	3.4994523357094616E-4
106-107	3.4994523357094616E-4	0.0	0.0	6.0120591127488545	3.4994523357094616E-4
108-109	3.4994523357094616E-4	0.0	0.0	6.3065380267988065	3.4994523357094616E-4
110-111	3.4994523357094616E-4	0.0	0.0	6.6115152978558855	3.4994523357094616E-4
112-113	3.4994523357094616E-4	0.0	0.0	6.9485125577847064	3.4994523357094616E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2075	0.0	46.788193	1
CTCAACG	270	0.0	46.325733	1
CCGCAGA	280	0.0	42.54404	1
GTATCAA	3145	0.0	40.149673	1
GTGTAAT	75	8.123453E-5	39.70777	1
ATCAACG	3830	0.0	33.87227	3
TATCAAC	3860	0.0	33.609013	2
CTAACTC	90	2.3862693E-4	33.060852	4
CAACGCA	4095	0.0	31.970938	5
TCAACGC	4150	0.0	31.690622	4
AACGCAG	4225	0.0	31.40427	6
TGTAAGT	140	3.0588963E-6	29.780828	1
GTACACT	140	3.084051E-6	29.749563	6
ACGCAGA	4515	0.0	29.255383	7
GAGTACT	2110	0.0	28.33963	12-13
CGCAGAG	4695	0.0	28.133772	8
AGCACCG	85	0.007195814	28.004488	5
GCAGAGT	4820	0.0	27.40416	9
GTACTTT	2355	0.0	25.138697	14-15
CATGGGT	450	0.0	23.803816	4
>>END_MODULE