FastQCFastQC Report
Sun 1 Jan 2017
ERR1142925_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1142925_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences482719
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA28650.5935129961737574No Hit
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC5540.11476656191283129No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24100.032.3910521
GTATCAA32400.029.2430671
ATGGGGG19800.027.058085
TGTACTA900.00950192926.4567895
TATCAAC37000.025.422612
CAACGCA36350.025.3832325
CATGGGG28650.025.348654
AACGCAG37350.024.993846
ATCAACG36850.024.8798623
TGGGGGG13450.024.7565236
TCAACGC37650.024.3486794
ACGCAGA40300.022.8668067
CGCAGAG41100.022.4240368
ATAGGAC1350.00254470222.0496143
GTACATG52400.021.9480931
TACATGG52950.021.6998182
ACATGGG52000.021.5238153
GCAGAGT42150.021.4422289
TGGGGGC5350.020.0051886
GTCTATA1500.00466121919.8632141
Produced by FastQC (version 0.11.5)