FastQCFastQC Report
Sun 1 Jan 2017
ERR1142925_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1142925_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences482719
Sequences flagged as poor quality0
Sequence length125
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30780.6376380461510734No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26340.5456590687335696No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16500.34181376742991265No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8840.1831293153988138No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6650.13776130626720723No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA5130.10627300769184556No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCAACG3800.042.313521
GGTATCA26700.040.37071
GTATCAA39500.035.882181
CGTATCA1102.0251113E-532.483111
TATCAAC48450.029.8467042
ATCAACG49600.028.9147363
CAACGCA52250.028.0177145
TCAACGC52700.027.5526334
AACGCAG54300.027.173516
GTATAAC1556.7741166E-626.8946111
ACGCAGA57800.025.6309917
CGCAGAG59600.024.8569018
GCAGAGT63000.023.6098589
GAGTACT23850.023.20002412-13
CCGCAGA3757.2759576E-1222.2328821
CATGGGG25350.021.8317664
AGTACTT25000.021.41886312-13
CAGAGTA62200.021.13955310-11
ATGGGGC6200.021.1161565
ACATGGG48300.020.4524253
Produced by FastQC (version 0.11.5)