FastQCFastQC Report
Sun 1 Jan 2017
ERR1142920_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1142920_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences719406
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21020.29218549747986533No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA35200.027.7549881
CATGGGG29750.027.210014
GGTATCA27150.026.1106071
GTACATG68200.024.5449311
TACATGG68800.024.1391962
ACATGGG67250.023.810423
ATGGGGG17450.023.194785
AACGCAG43250.022.6903956
CATGGGT17700.022.1946074
CAACGCA43600.022.1159345
ATCAACG44050.021.6212653
TCAACGC44650.021.5958544
TATCAAC44750.021.4160752
TGGGGGG11200.020.7105126
ATTTAGG2951.1416341E-720.1937891
TAGCCCG1805.7664164E-419.8406335
ACGCAGA48600.019.7003257
TTAGGAC1550.005671771719.201953
GTAAGTA3101.9408253E-719.201952
CGCAGAG50250.018.9351068
Produced by FastQC (version 0.11.5)