##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1142920_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	719406
Sequences flagged as poor quality	0
Sequence length	125
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.5785814964012	33.0	33.0	33.0	27.0	33.0
2	31.603203198194066	33.0	33.0	33.0	27.0	33.0
3	31.666380319318993	33.0	33.0	33.0	33.0	33.0
4	31.61459593053158	33.0	33.0	33.0	33.0	33.0
5	31.69837199022527	33.0	33.0	33.0	33.0	33.0
6	35.16822628668652	37.0	37.0	37.0	33.0	37.0
7	35.15484580334331	37.0	37.0	37.0	33.0	37.0
8	35.13773446426635	37.0	37.0	37.0	33.0	37.0
9	35.05219027920256	37.0	37.0	37.0	33.0	37.0
10-11	35.09494986141344	37.0	37.0	37.0	33.0	37.0
12-13	35.05745156420714	37.0	37.0	37.0	33.0	37.0
14-15	35.06023094052593	37.0	37.0	37.0	33.0	37.0
16-17	35.07662502119804	37.0	37.0	37.0	33.0	37.0
18-19	35.06808394703408	37.0	37.0	37.0	33.0	37.0
20-21	35.076399140401946	37.0	37.0	37.0	33.0	37.0
22-23	35.12697211310442	37.0	37.0	37.0	33.0	37.0
24-25	35.195944987948394	37.0	37.0	37.0	33.0	37.0
26-27	35.24561026735946	37.0	37.0	37.0	33.0	37.0
28-29	35.307847029354775	37.0	37.0	37.0	33.0	37.0
30-31	35.311281112473345	37.0	37.0	37.0	33.0	37.0
32-33	35.35697158489087	37.0	37.0	37.0	33.0	37.0
34-35	35.36685543351042	37.0	37.0	37.0	33.0	37.0
36-37	35.34477541193707	37.0	37.0	37.0	33.0	37.0
38-39	35.336775756665915	37.0	37.0	37.0	33.0	37.0
40-41	35.30048262038404	37.0	37.0	37.0	33.0	37.0
42-43	35.305684133854875	37.0	37.0	37.0	33.0	37.0
44-45	35.286581568683054	37.0	37.0	37.0	33.0	37.0
46-47	35.286777563712285	37.0	37.0	37.0	33.0	37.0
48-49	35.24828052587829	37.0	37.0	37.0	33.0	37.0
50-51	35.2276829773452	37.0	37.0	37.0	33.0	37.0
52-53	35.213663772612406	37.0	37.0	37.0	33.0	37.0
54-55	35.19033688904457	37.0	37.0	37.0	33.0	37.0
56-57	35.16161527704801	37.0	37.0	37.0	33.0	37.0
58-59	35.11651348473602	37.0	37.0	37.0	33.0	37.0
60-61	35.08379899528222	37.0	37.0	37.0	33.0	37.0
62-63	35.054748639850104	37.0	37.0	37.0	33.0	37.0
64-65	35.020837329685875	37.0	37.0	37.0	33.0	37.0
66-67	34.99228808211219	37.0	37.0	37.0	30.0	37.0
68-69	34.93336864023931	37.0	37.0	37.0	27.0	37.0
70-71	34.869412404122286	37.0	37.0	37.0	27.0	37.0
72-73	34.83206631582166	37.0	37.0	37.0	27.0	37.0
74-75	34.797681698512385	37.0	37.0	37.0	27.0	37.0
76-77	34.723367611612915	37.0	37.0	37.0	27.0	37.0
78-79	34.68772848711298	37.0	37.0	37.0	27.0	37.0
80-81	34.63491269185967	37.0	37.0	37.0	27.0	37.0
82-83	34.56563470418651	37.0	37.0	37.0	27.0	37.0
84-85	34.50938899592163	37.0	37.0	37.0	27.0	37.0
86-87	34.445475850910334	37.0	37.0	37.0	27.0	37.0
88-89	34.37135080886175	37.0	37.0	37.0	27.0	37.0
90-91	34.29058973653264	37.0	37.0	37.0	27.0	37.0
92-93	34.25035445909542	37.0	37.0	37.0	27.0	37.0
94-95	34.193381067158185	37.0	37.0	37.0	27.0	37.0
96-97	34.1082601479554	37.0	37.0	37.0	27.0	37.0
98-99	34.012190612811125	37.0	37.0	37.0	22.0	37.0
100-101	33.91598068406435	37.0	37.0	37.0	22.0	37.0
102-103	33.84512083580064	37.0	35.0	37.0	22.0	37.0
104-105	33.695842820326774	37.0	33.0	37.0	22.0	37.0
106-107	33.64462556609203	37.0	33.0	37.0	22.0	37.0
108-109	33.53416082156668	37.0	33.0	37.0	22.0	37.0
110-111	33.41267448422727	37.0	33.0	37.0	22.0	37.0
112-113	33.292646989321746	37.0	33.0	37.0	22.0	37.0
114-115	33.166491522172464	37.0	33.0	37.0	22.0	37.0
116-117	33.06777327406221	37.0	33.0	37.0	14.0	37.0
118-119	32.941744300158746	37.0	33.0	37.0	14.0	37.0
120-121	32.86381056037898	37.0	33.0	37.0	14.0	37.0
122-123	32.68697439276292	37.0	33.0	37.0	14.0	37.0
124-125	30.636436170952145	35.0	27.5	37.0	14.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	486.0
1	61.0
2	33.0
3	46.0
4	19.0
5	36.0
6	33.0
7	40.0
8	30.0
9	33.0
10	15.0
11	26.0
12	23.0
13	29.0
14	55.0
15	210.0
16	331.0
17	571.0
18	1252.0
19	2250.0
20	3635.0
21	4706.0
22	5169.0
23	5542.0
24	6017.0
25	6652.0
26	7837.0
27	9327.0
28	11347.0
29	13692.0
30	17039.0
31	20625.0
32	25856.0
33	35622.0
34	54622.0
35	109491.0
36	376648.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.375577370418604	19.523050296615356	11.969097465691677	24.13227486727437
2	17.696034561708487	23.086558763394542	35.42491610796637	23.792490566930603
3	19.842837829747477	27.69983250700672	27.757612716658382	24.699716946587415
4	14.513287891552423	19.50204348764508	33.57641669045513	32.40825193034736
5	15.484455933291644	38.073179190268405	30.455117125423577	15.987247751016378
6	29.916142732694873	36.004213737228675	18.111555492470103	15.968088037606352
7	26.732356427708797	33.43026329702866	21.117328851366015	18.72005142389653
8	26.220253580143176	33.608133261735105	20.124898097731577	20.046715060390135
9	27.103554017052154	18.316878520068563	19.40323011509094	35.17633734778834
10-11	25.547294448935187	27.271437177484092	25.14333653791305	22.037931835667667
12-13	26.142680935641433	26.086052130502264	24.988538580286637	22.782728353569667
14-15	24.932967851005657	26.514815252857648	22.852014443045285	25.700202453091407
16-17	24.368344194582754	28.281037698634943	23.735453497456817	23.615164609325483
18-19	24.42493367629859	27.405586567302294	25.171766732601903	22.997713023797214
20-21	26.34945968736172	26.002423962441107	24.450016558641245	23.19809979155593
22-23	25.904414967112814	25.78029663168997	24.56868182709982	23.746606574097395
24-25	24.875904284919308	26.404702281580413	24.50674735670562	24.21264607679466
26-27	24.62572907773884	26.395692918683523	24.66552699892365	24.31305100465399
28-29	24.301943612755355	26.380613553451266	25.143667863695917	24.173774970097462
30-31	24.894776063842	26.121698640316787	25.068589515667217	23.914935780173995
32-33	24.382158744340327	26.271604525930304	24.714346052335852	24.63189067739352
34-35	24.74491748701797	26.365183794414143	24.93496805118491	23.954930667382982
36-37	24.577448765380126	26.56205315611382	24.562140828841265	24.29835724966479
38-39	24.58565957707358	26.30139769444429	24.536397944927167	24.576544783554965
40-41	24.293250193120056	26.67715198374312	24.797450119351673	24.232147703785152
42-43	24.63308495364716	26.38085952315833	24.804981234076106	24.18107428911841
44-45	24.41498805373696	26.48681039300526	24.62196845741138	24.476233095846403
46-47	24.257220065236165	26.25160672070305	24.955860850853867	24.535312363206913
48-49	24.464136348263708	26.6840683467105	24.76350462691698	24.088290678108816
50-51	24.18821882076857	26.88875093778142	24.718108820225734	24.204921421224274
52-53	24.349038356467577	26.320210974839902	24.80283749745086	24.527913171241664
54-55	24.268758420273233	26.249540712369036	25.015518354803874	24.46618251255386
56-57	24.48216688957765	25.95449275806063	24.562679274010712	25.000661078351005
58-59	24.434153586187794	26.154137384349635	24.892648903104934	24.519060126357633
60-61	24.53325038655149	26.18821960856356	25.094811233443696	24.183718771441256
62-63	24.034410930989534	26.325364386036583	24.889534118976748	24.750690563997136
64-65	24.12843800474464	25.95183400847188	25.130950898023574	24.7887770887599
66-67	24.55512324187475	25.954385996655464	24.89131652622078	24.599174235249006
68-69	24.616966953070143	25.76476130762545	24.729143774917066	24.889127964387338
70-71	24.18383666015731	25.902111898760253	25.123424914364612	24.790626526717823
72-73	24.55227962637427	26.126381923885795	24.77546129082095	24.545877158918987
74-75	24.59597379710855	25.828027516933606	25.11546588640774	24.46053279955011
76-77	24.433743460380835	25.648393321026735	25.131697280019598	24.786165938572825
78-79	24.60103569241049	25.79326521521243	25.050253354863855	24.555445737513224
80-81	24.306110459920205	25.774839839519338	25.398278257431695	24.520771443128762
82-83	24.507900666202158	25.921048326977115	25.118348158320984	24.45270284849974
84-85	24.321159712466603	26.110168229035413	24.768150789352408	24.800521269145573
86-87	24.062132381091597	26.11133153743883	25.042495875707395	24.784040205762174
88-89	24.298800011136787	25.60639808447253	25.152156360497813	24.942645543892862
90-91	24.624564422336658	25.706147439993654	25.013051667898743	24.656236469770946
92-93	24.395474963819343	26.034342215084706	24.951463271845356	24.618719549250596
94-95	23.64373191074086	26.286938980639356	25.2086485974777	24.86068051114208
96-97	24.051230358400883	26.082498196996575	25.014827788807736	24.85144365579481
98-99	23.746736201530418	26.312725854854097	24.89823911544969	25.042298828165798
100-101	23.999894198161275	26.605334083227067	24.639716896553647	24.75505482205801
102-103	24.29305420492048	26.39932561446838	24.9792038937272	24.32841628688394
104-105	24.056961055958563	26.366661561381772	25.03543531836093	24.540942064298743
106-107	24.09755982097046	26.39049687032472	25.082096976422484	24.429846332282334
108-109	23.942777584406542	26.44852071005917	24.976331360946745	24.63237034458754
110-111	23.853958199189044	26.720080747611508	24.838313349459654	24.587647703739798
112-113	23.97869105369565	26.894538538226332	24.71212593776629	24.414644470311725
114-115	23.957155587735667	26.850093291375423	24.740106936979586	24.452644183909324
116-117	24.03424184538959	26.931585923446626	24.701563813968512	24.33260841719527
118-119	23.714416193051292	26.60497832182826	25.029029536334956	24.651575948785492
120-121	23.722898283601467	26.529803970428862	25.262081576088054	24.48521616988162
122-123	23.891821911944344	26.740353795664383	25.04487699284126	24.322947299550012
124-125	23.943664913737944	26.557601219275302	24.81342205431735	24.685311812669404
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1367.0
1	1099.0
2	570.5
3	266.5
4	208.5
5	179.0
6	167.0
7	179.5
8	195.5
9	195.0
10	180.0
11	167.5
12	164.5
13	177.5
14	188.5
15	209.5
16	238.5
17	257.0
18	305.0
19	357.0
20	426.0
21	552.0
22	688.0
23	883.0
24	1098.5
25	1363.0
26	1737.0
27	2148.0
28	2704.0
29	3714.5
30	4887.5
31	5774.5
32	7091.5
33	8484.0
34	9600.0
35	10802.5
36	11920.5
37	13232.0
38	14119.5
39	14790.0
40	15235.0
41	15525.5
42	16349.0
43	18111.0
44	20283.5
45	21751.0
46	23914.5
47	26204.0
48	28157.5
49	29993.5
50	31218.0
51	30896.0
52	30324.0
53	30637.0
54	29982.0
55	29271.0
56	28445.0
57	26312.5
58	24125.5
59	21755.5
60	19073.0
61	15971.0
62	12643.0
63	9816.5
64	7623.0
65	6152.0
66	4832.0
67	3660.0
68	3006.5
69	2590.0
70	2174.0
71	2007.0
72	1858.5
73	1784.5
74	1434.0
75	1178.5
76	946.0
77	652.5
78	473.5
79	303.5
80	218.0
81	161.5
82	120.5
83	85.5
84	66.0
85	36.0
86	20.0
87	11.5
88	3.5
89	2.5
90	2.5
91	1.5
92	0.0
93	0.0
94	0.5
95	1.0
96	0.5
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.08757224710386069
2	0.1283002921854975
3	0.16221716249238954
4	0.17667353344286815
5	0.19669004706660775
6	0.11175886773254601
7	0.09368840404444778
8	0.08062206876228444
9	0.09313238977712168
10-11	0.08937929347267051
12-13	0.09598196289716794
14-15	0.10022157168552943
16-17	0.10001306633528216
18-19	0.10724125181052145
20-21	0.10397466798998062
22-23	0.10244562875483385
24-25	0.08423616149990408
26-27	0.10779726607784755
28-29	0.11419143015209769
30-31	0.11391342301843466
32-33	0.11613748008773907
34-35	0.12691025651718224
36-37	0.11460844085259228
38-39	0.11036883206423076
40-41	0.13080235638846494
42-43	0.13198388670653288
44-45	0.13629299727831015
46-47	0.13059385103821763
48-49	0.12413018518055174
50-51	0.13274840632410628
52-53	0.1426871613525603
54-55	0.12538121728203547
56-57	0.1227401495122365
58-59	0.13469445625974763
60-61	0.12135011384392125
62-63	0.13504196517682643
64-65	0.12670175116693494
66-67	0.12795278326841866
68-69	0.12475570123129359
70-71	0.13247039919044323
72-73	0.12969032785381274
74-75	0.1406716096335032
76-77	0.15179189498002518
78-79	0.14539773090577504
80-81	0.1478997951087425
82-83	0.15019335396146263
84-85	0.16110513395773737
86-87	0.1530429270815089
88-89	0.14817780224240554
90-91	0.15387694848249805
92-93	0.15783855013719653
94-95	0.1519308985468567
96-97	0.16124413752456887
98-99	0.1808436404478139
100-101	0.15040185931170993
102-103	0.1560315037683867
104-105	0.16624826593050376
106-107	0.16687378198124564
108-109	0.16068812325724277
110-111	0.1554754895010606
112-113	0.1681943158661451
114-115	0.1704183729354495
116-117	0.16152214465823192
118-119	0.16291218032654717
120-121	0.14880331829314739
122-123	0.1464402576570115
124-125	0.17716004592677848
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	719406.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	76.89683919784093
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.06590546640878	66.18196093067016
2	9.835739280299856	15.126745236582115
3	2.1580768943024498	4.978478757532542
4	0.7545006528284306	2.320748615008554
5	0.37051339250399895	1.4245654382013264
6	0.18713782003192225	0.8634184112897533
7	0.1368445729237642	0.736604058345114
8	0.09207644723172921	0.5664310205349414
9	0.05988922272596133	0.4144762736677737
>10	0.3069819944318207	4.1872916702513665
>50	0.02081797527007252	1.080393561667739
>100	0.010630413268799706	1.4448343551570326
>500	6.644008292668506E-4	0.31607880281606615
>1k	2.214669430889502E-4	0.3579728682755232
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2102	0.29218549747986533	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	6.950178341576245E-5
14-15	0.0	0.0	0.0	0.0	1.390035668315249E-4
16-17	0.0	0.0	0.0	0.0	1.390035668315249E-4
18-19	0.0	0.0	0.0	1.390035668315249E-4	2.780071336630498E-4
20-21	0.0	0.0	0.0	2.780071336630498E-4	2.780071336630498E-4
22-23	0.0	0.0	0.0	0.0011120285346521992	2.780071336630498E-4
24-25	0.0	0.0	0.0	0.00368359452103541	2.780071336630498E-4
26-27	0.0	0.0	0.0	0.008270712226475732	2.780071336630498E-4
28-29	0.0	0.0	0.0	0.017444947637356375	2.780071336630498E-4
30-31	0.0	0.0	0.0	0.033847368523476314	2.780071336630498E-4
32-33	0.0	0.0	0.0	0.06373313539225417	2.780071336630498E-4
34-35	0.0	0.0	0.0	0.09917904493429303	2.780071336630498E-4
36-37	0.0	0.0	0.0	0.15262591638101436	2.780071336630498E-4
38-39	0.0	0.0	0.0	0.2235872372485078	2.780071336630498E-4
40-41	0.0	0.0	0.0	0.3066418684303439	2.780071336630498E-4
42-43	0.0	0.0	0.0	0.3801052535008048	2.780071336630498E-4
44-45	0.0	0.0	0.0	0.4624648668484833	2.780071336630498E-4
46-47	0.0	0.0	0.0	0.5473265443991293	2.780071336630498E-4
48-49	0.0	0.0	0.0	0.6406674395264982	2.780071336630498E-4
50-51	0.0	0.0	0.0	0.7326878007689677	2.780071336630498E-4
52-53	0.0	0.0	0.0	0.820955065706986	2.780071336630498E-4
54-55	0.0	0.0	0.0	0.9087358181610941	2.780071336630498E-4
56-57	0.0	0.0	0.0	1.0088878880632077	2.780071336630498E-4
58-59	0.0	0.0	0.0	1.1149476095556612	2.780071336630498E-4
60-61	0.0	0.0	0.0	1.2312935949936477	2.780071336630498E-4
62-63	0.0	0.0	0.0	1.341175914573968	2.780071336630498E-4
64-65	0.0	0.0	0.0	1.449668198485973	2.780071336630498E-4
66-67	0.0	0.0	0.0	1.569419771311332	2.780071336630498E-4
68-69	0.0	0.0	0.0	1.7082843345760252	2.780071336630498E-4
70-71	0.0	0.0	0.0	1.8609797527404552	2.780071336630498E-4
72-73	0.0	0.0	0.0	1.9992883017378225	2.780071336630498E-4
74-75	0.0	0.0	0.0	2.1348862811819753	2.780071336630498E-4
76-77	0.0	0.0	0.0	2.291821308134767	2.780071336630498E-4
78-79	0.0	0.0	0.0	2.4602241293511593	2.780071336630498E-4
80-81	0.0	0.0	0.0	2.641484780499468	4.1701070049457467E-4
82-83	0.0	0.0	0.0	2.8378968204324124	5.560142673260996E-4
84-85	0.0	0.0	0.0	3.0198524894148786	5.560142673260996E-4
86-87	0.0	0.0	0.0	3.2044492261671436	5.560142673260996E-4
88-89	0.0	0.0	0.0	3.41198155144661	5.560142673260996E-4
90-91	0.0	0.0	0.0	3.625977542583743	5.560142673260996E-4
92-93	0.0	0.0	0.0	3.8424755979238427	5.560142673260996E-4
94-95	0.0	0.0	0.0	4.0572361086785484	5.560142673260996E-4
96-97	0.0	0.0	0.0	4.2748461925533014	5.560142673260996E-4
98-99	0.0	0.0	0.0	4.516851402406986	5.560142673260996E-4
100-101	0.0	0.0	0.0	4.785684300659154	5.560142673260996E-4
102-103	0.0	0.0	0.0	5.064525455723194	6.950178341576245E-4
104-105	0.0	0.0	0.0	5.351498319446877	8.340214009891493E-4
106-107	0.0	0.0	0.0	5.638679688520807	8.340214009891493E-4
108-109	0.0	0.0	0.0	5.937050844724675	9.035231844049117E-4
110-111	0.0	0.0	0.0	6.251685418247832	9.730249678206743E-4
112-113	0.0	0.0	0.0	6.581818889472704	9.730249678206743E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	3520	0.0	27.754988	1
CATGGGG	2975	0.0	27.21001	4
GGTATCA	2715	0.0	26.110607	1
GTACATG	6820	0.0	24.544931	1
TACATGG	6880	0.0	24.139196	2
ACATGGG	6725	0.0	23.81042	3
ATGGGGG	1745	0.0	23.19478	5
AACGCAG	4325	0.0	22.690395	6
CATGGGT	1770	0.0	22.194607	4
CAACGCA	4360	0.0	22.115934	5
ATCAACG	4405	0.0	21.621265	3
TCAACGC	4465	0.0	21.595854	4
TATCAAC	4475	0.0	21.416075	2
TGGGGGG	1120	0.0	20.710512	6
ATTTAGG	295	1.1416341E-7	20.193789	1
TAGCCCG	180	5.7664164E-4	19.840633	5
ACGCAGA	4860	0.0	19.700325	7
TTAGGAC	155	0.0056717717	19.20195	3
GTAAGTA	310	1.9408253E-7	19.20195	2
CGCAGAG	5025	0.0	18.935106	8
>>END_MODULE