FastQCFastQC Report
Sun 1 Jan 2017
ERR1142920_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1142920_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences719406
Sequences flagged as poor quality0
Sequence length125
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33270.4624648668484833No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26880.37364158764313893No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17700.24603631329179906No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8410.11690199970531244No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA7960.11064683919789382No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTTAG350.007791979251.0051232
CCGCAGA4250.042.0246661
CTCAACG3150.041.5799641
GGTATCA32500.039.3846551
GTATCAA48850.036.927511
CATGGGT16050.034.1069764
GTCGACG902.3926483E-433.054289
ATCAACG59850.030.7223833
ATGGGTA6300.030.2210585
TATCAAC62050.030.1126142
CAACGCA62100.029.8925695
AACGCAG64650.029.5417846
TCAACGC63100.029.3265824
ACGCAGA70150.027.0559777
CGCAGAG72800.026.2345668
GAGTACT29350.025.44109712-13
ATGGGGG14550.024.9440565
GCAGAGT77050.024.7102749
CATGGGG26050.024.6687094
ACATGGG64100.024.0437563
Produced by FastQC (version 0.11.5)