##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1142920_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	719406
Sequences flagged as poor quality	0
Sequence length	125
%GC	48
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	22.512143351598404	14.0	14.0	33.0	14.0	33.0
2	27.336972446712984	27.0	27.0	33.0	14.0	33.0
3	31.041969346933442	33.0	33.0	33.0	27.0	33.0
4	30.783902274932373	33.0	33.0	33.0	27.0	33.0
5	32.12008101127875	33.0	33.0	33.0	33.0	33.0
6	35.25976430555208	37.0	37.0	37.0	33.0	37.0
7	35.47578001851527	37.0	37.0	37.0	33.0	37.0
8	35.74654784641774	37.0	37.0	37.0	33.0	37.0
9	36.04426429582183	37.0	37.0	37.0	33.0	37.0
10-11	36.093448622891664	37.0	37.0	37.0	37.0	37.0
12-13	36.168445217304274	37.0	37.0	37.0	37.0	37.0
14-15	36.153107563740086	37.0	37.0	37.0	37.0	37.0
16-17	36.16015017945361	37.0	37.0	37.0	37.0	37.0
18-19	36.15323475200374	37.0	37.0	37.0	37.0	37.0
20-21	36.07936130641112	37.0	37.0	37.0	37.0	37.0
22-23	36.12471261012558	37.0	37.0	37.0	37.0	37.0
24-25	36.15414036024164	37.0	37.0	37.0	37.0	37.0
26-27	36.09214963455962	37.0	37.0	37.0	37.0	37.0
28-29	36.0919029032285	37.0	37.0	37.0	37.0	37.0
30-31	36.05553679007403	37.0	37.0	37.0	37.0	37.0
32-33	36.01160471277693	37.0	37.0	37.0	37.0	37.0
34-35	35.95178869789799	37.0	37.0	37.0	37.0	37.0
36-37	35.89484936183463	37.0	37.0	37.0	35.0	37.0
38-39	35.84778066905197	37.0	37.0	37.0	33.0	37.0
40-41	35.77881265933284	37.0	37.0	37.0	33.0	37.0
42-43	35.702915321807154	37.0	37.0	37.0	33.0	37.0
44-45	35.61750736023886	37.0	37.0	37.0	33.0	37.0
46-47	35.49093835747826	37.0	37.0	37.0	33.0	37.0
48-49	35.289894023680645	37.0	37.0	37.0	33.0	37.0
50-51	35.22870534857925	37.0	37.0	37.0	33.0	37.0
52-53	35.19592205235986	37.0	37.0	37.0	33.0	37.0
54-55	35.11817318732399	37.0	37.0	37.0	33.0	37.0
56-57	35.08813521154953	37.0	37.0	37.0	33.0	37.0
58-59	35.03927128770124	37.0	37.0	37.0	33.0	37.0
60-61	35.006687461600265	37.0	37.0	37.0	33.0	37.0
62-63	34.98491672296311	37.0	37.0	37.0	27.0	37.0
64-65	34.94973978532289	37.0	37.0	37.0	27.0	37.0
66-67	34.92741998259675	37.0	37.0	37.0	27.0	37.0
68-69	34.848942738870676	37.0	37.0	37.0	27.0	37.0
70-71	34.81943714675718	37.0	37.0	37.0	27.0	37.0
72-73	34.80790680088851	37.0	37.0	37.0	27.0	37.0
74-75	34.74666738948521	37.0	37.0	37.0	27.0	37.0
76-77	34.69684781611496	37.0	37.0	37.0	27.0	37.0
78-79	34.605120057380674	37.0	37.0	37.0	27.0	37.0
80-81	34.51708840348843	37.0	37.0	37.0	27.0	37.0
82-83	34.526423188019	37.0	37.0	37.0	27.0	37.0
84-85	34.48132139570701	37.0	37.0	37.0	27.0	37.0
86-87	34.39897081759118	37.0	37.0	37.0	27.0	37.0
88-89	34.339603089215274	37.0	37.0	37.0	27.0	37.0
90-91	34.27084497488205	37.0	37.0	37.0	27.0	37.0
92-93	34.17961901902403	37.0	37.0	37.0	27.0	37.0
94-95	34.114199075348274	37.0	37.0	37.0	27.0	37.0
96-97	34.052063090938915	37.0	37.0	37.0	27.0	37.0
98-99	33.9520799103705	37.0	37.0	37.0	22.0	37.0
100-101	33.889526915260646	37.0	37.0	37.0	22.0	37.0
102-103	33.7985386555019	37.0	33.0	37.0	22.0	37.0
104-105	33.64389440733049	37.0	33.0	37.0	22.0	37.0
106-107	33.55399732557137	37.0	33.0	37.0	22.0	37.0
108-109	33.486742534813445	37.0	33.0	37.0	22.0	37.0
110-111	33.437934212391895	37.0	33.0	37.0	22.0	37.0
112-113	33.35825736788406	37.0	33.0	37.0	22.0	37.0
114-115	33.19326360914421	37.0	33.0	37.0	14.0	37.0
116-117	33.145344909550374	37.0	33.0	37.0	14.0	37.0
118-119	32.966357661737604	37.0	33.0	37.0	14.0	37.0
120-121	32.85777085540015	37.0	33.0	37.0	14.0	37.0
122-123	32.78350680978474	37.0	33.0	37.0	14.0	37.0
124-125	31.059501866817904	35.0	27.5	37.0	14.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	2.0
15	19.0
16	17.0
17	25.0
18	83.0
19	496.0
20	1674.0
21	3657.0
22	5839.0
23	7832.0
24	6982.0
25	5630.0
26	5627.0
27	6468.0
28	8308.0
29	10557.0
30	13916.0
31	19365.0
32	28061.0
33	42133.0
34	71299.0
35	155789.0
36	325627.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.903606633970526	13.733492596089533	12.205432269063209	31.157468500876735
2	16.92614359824075	20.233223426318744	38.07388420285906	24.766748772581444
3	19.89054798290803	26.382189651960935	28.444041022960736	25.283221342170304
4	14.150556527855585	18.496436470194098	33.56168096851277	33.79132603343755
5	16.42097273944211	35.08676088524877	31.556274682335477	16.93599169297364
6	30.395492948349055	34.195850465522945	18.843740530382007	16.56491605574599
7	26.905113385209468	31.62748156117686	22.33509311848942	19.132311935124253
8	26.831719501922418	30.85350970105893	21.105606569864584	21.20916422715407
9	27.480727155458812	15.52753243648232	20.612560918313164	36.3791794897457
10-11	25.819426026471838	24.839033869609096	26.417975385248386	22.92356471867068
12-13	26.941650067208688	23.76643902357656	26.563279728551947	22.728631180662802
14-15	25.131358370655793	24.1084311223426	25.170974387202776	25.589236119798837
16-17	23.986942004931848	26.06358579161141	26.025220807165912	23.92425139629083
18-19	23.9011042474048	24.76730738222195	28.407820918426918	22.923767451946333
20-21	25.41779520893409	23.681212785906062	28.093005088257396	22.807986916902454
22-23	24.92737063633053	23.590990344812248	28.23739307150622	23.244245947351008
24-25	24.043010573328356	24.30287828335797	28.071203335536545	23.582907807777126
26-27	23.817682034744113	24.37508904959324	28.34164462870229	23.465584286960357
28-29	23.558602513740503	24.32527668659978	28.735373349680156	23.380747449979566
30-31	24.041128689791012	24.11035299530651	28.671025388500563	23.177492926401914
32-33	23.674183979560915	24.197740914031854	28.203476201199322	23.924598905207908
34-35	23.89714570075868	24.404161210776667	28.437419204176777	23.26127388428787
36-37	23.847865733301454	24.46685033722359	28.062604600474838	23.622679329000118
38-39	23.88373185655944	24.102870979669337	28.11020480785537	23.903192355915852
40-41	23.58346839996775	24.520084848720735	28.31902507374221	23.5774216775693
42-43	24.05319392192581	24.1940046329921	28.107166264366896	23.64563518071519
44-45	23.792615018501376	24.511124455453526	27.753104644665182	23.943155881379916
46-47	23.720680672666063	24.34689174124208	27.800921871655227	24.13150571443663
48-49	23.99653325104322	24.665696421770182	27.55189698167655	23.785873345510044
50-51	23.77454114795025	24.994370339892466	27.26215033555554	23.968938176601743
52-53	24.011834837708847	24.556176210363752	27.171475177630295	24.260513774297106
54-55	23.88143829770672	24.481447193934997	27.391834374470047	24.245280133888237
56-57	24.057972827582756	24.339038039716097	26.934860148511408	24.668128984189735
58-59	23.96650848060761	24.501672212908986	27.22280603720291	24.30901326928049
60-61	23.922060591739005	24.4663993161015	27.59871004510672	24.012830047052773
62-63	23.483413689257837	24.543458573665543	27.496738617479622	24.476389119597002
64-65	23.567081731317224	24.313530885202514	27.642040794766793	24.477346588713466
66-67	23.870040005226535	24.341818111052728	27.519856659521885	24.268285224198852
68-69	23.97186011793063	24.129698668067824	27.542931251615915	24.355509962385636
70-71	23.437252399896582	24.261682554774357	28.038131458453226	24.26293358687584
72-73	23.775239572647433	24.309986294248308	27.851519169981902	24.063254963122354
74-75	23.870526517710445	24.18974820893904	27.956049852239207	23.983675421111307
76-77	23.5483326163061	24.05826755564143	28.056985941864497	24.336413886187973
78-79	23.63470696658076	24.25883298165431	27.958273909308513	24.148186142456414
80-81	23.43058022868867	24.280239530946364	28.23746257328963	24.051717667075337
82-83	23.4487896959436	24.307136721128263	28.136476481986527	24.10759710094161
84-85	23.512661834902683	24.37184288148834	27.681448305963528	24.434046977645448
86-87	23.21373466443149	24.428903845672682	27.92811013530607	24.42925135458976
88-89	23.2618298985552	24.108848133043093	27.89210821149671	24.737213756905003
90-91	23.77204249061031	24.051370158158257	27.815725751522784	24.36086159970865
92-93	23.46317656511066	24.36266864607746	27.70174282672093	24.47241196209095
94-95	22.968879881457756	24.470465912155305	27.930195188808543	24.630459017578392
96-97	23.228747049649296	24.296641951832484	27.602494280003224	24.872116718515
98-99	22.92898010019405	24.395888819078017	27.50485123796921	25.17027984275873
100-101	23.19680847366921	24.67281989977829	27.187467420993737	24.94290420555876
102-103	23.515474593587758	24.410658808320765	27.454076632772917	24.61978996531856
104-105	23.363346666156986	24.439170005664423	27.256438502785297	24.941044825393295
106-107	23.362169683397866	24.451681911914648	27.312867795516986	24.8732806091705
108-109	23.33551338018233	24.546864172887677	27.00786137404113	25.10976107288886
110-111	23.213317653730993	24.876912341570684	26.87467160407336	25.03509840062496
112-113	23.281099918126102	24.81627024780407	26.805013629432345	25.09761620463748
114-115	23.279003449395088	24.765725398126083	26.86865401309283	25.086617139386004
116-117	23.206811759194775	24.8606830941767	26.822657119861887	25.10984802676664
118-119	22.886955627267096	24.658653909642066	27.244300588898025	25.21008987419281
120-121	22.977272727272727	24.46768140116764	27.304767862107308	25.25027800945232
122-123	23.006237945474446	24.730621535681397	27.062883355632394	25.20025716321176
124-125	22.954185458642364	24.58563823909023	27.089914434391936	25.37026186787547
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	217.0
1	216.5
2	509.5
3	694.0
4	515.0
5	492.5
6	618.0
7	1206.0
8	2122.5
9	2385.5
10	1995.0
11	1622.0
12	1427.5
13	1334.5
14	1282.5
15	1235.0
16	1210.0
17	1185.0
18	1148.0
19	1120.5
20	1071.0
21	1050.0
22	1106.5
23	1187.0
24	1257.0
25	1381.5
26	1673.5
27	2043.0
28	2432.5
29	3133.5
30	4110.5
31	5095.0
32	6386.0
33	7735.0
34	8728.0
35	9791.5
36	11048.5
37	12486.5
38	13620.0
39	14164.5
40	14371.5
41	14637.5
42	15468.0
43	17031.0
44	19034.0
45	20861.0
46	23046.0
47	25383.5
48	27637.0
49	29721.0
50	31022.5
51	30562.5
52	29826.0
53	30109.5
54	29758.0
55	29165.5
56	28291.0
57	26262.0
58	24433.5
59	22326.5
60	19503.5
61	15831.0
62	12373.5
63	9682.5
64	7344.5
65	6147.0
66	5000.0
67	3788.0
68	3058.5
69	2557.0
70	2113.0
71	1909.5
72	1809.5
73	1830.0
74	1491.5
75	1160.0
76	906.5
77	623.0
78	466.5
79	290.5
80	195.0
81	142.5
82	110.0
83	86.0
84	52.0
85	28.0
86	16.5
87	7.5
88	4.0
89	1.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03600192380936495
2	0.001390035668315249
3	5.560142673260996E-4
4	0.004865124839103371
5	0.0020850535024728735
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	6.950178341576245E-4
14-15	0.0
16-17	0.0
18-19	2.780071336630498E-4
20-21	6.25516050741862E-4
22-23	0.0
24-25	2.0850535024728733E-4
26-27	4.8651248391033713E-4
28-29	0.0
30-31	7.645196175733869E-4
32-33	0.0
34-35	0.0
36-37	2.780071336630498E-4
38-39	0.0
40-41	0.0011120285346521992
42-43	6.950178341576245E-5
44-45	0.0
46-47	0.0
48-49	0.0
50-51	2.780071336630498E-4
52-53	6.25516050741862E-4
54-55	0.0
56-57	0.0
58-59	0.0
60-61	1.390035668315249E-4
62-63	3.4750891707881226E-4
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	6.950178341576245E-5
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	2.780071336630498E-4
100-101	1.390035668315249E-4
102-103	1.390035668315249E-4
104-105	4.8651248391033713E-4
106-107	2.0850535024728733E-4
108-109	3.4750891707881226E-4
110-111	0.0
112-113	9.730249678206743E-4
114-115	6.25516050741862E-4
116-117	4.1701070049457467E-4
118-119	0.0015985410185625365
120-121	8.340214009891493E-4
122-123	0.002571565986383211
124-125	0.0022935588527201608
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	719406.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	75.92014358931371
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.49668930652327	65.66841072151503
2	9.507256223943578	14.435845153243859
3	2.1321386036412204	4.856168068222809
4	0.6976580706061671	2.118652035866551
5	0.3479050727432022	1.3206501539057276
6	0.2029829159107684	0.9246295273273877
7	0.1204471451966681	0.6401055190777772
8	0.08353217475662837	0.5073419761480698
9	0.06610303496938484	0.4516696715908611
>10	0.3078417434944069	4.227839851597148
>50	0.023868936766242688	1.2716508022126574
>100	0.011824929971365772	1.6869394665419868
>500	0.0010949009231858086	0.5958402530615
>1k	6.569405539114852E-4	1.2942567996885541
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3327	0.4624648668484833	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2688	0.37364158764313893	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1770	0.24603631329179906	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	841	0.11690199970531244	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	796	0.11064683919789382	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	2.780071336630498E-4
18-19	0.0	0.0	0.0	1.390035668315249E-4	2.780071336630498E-4
20-21	0.0	0.0	0.0	2.780071336630498E-4	2.780071336630498E-4
22-23	0.0	0.0	0.0	0.0012510321014837242	2.780071336630498E-4
24-25	0.0	0.0	0.0	0.0038225980878669346	2.780071336630498E-4
26-27	0.0	0.0	0.0	0.008409715793307257	2.780071336630498E-4
28-29	0.0	0.0	0.0	0.0175839512041879	2.780071336630498E-4
30-31	0.0	0.0	0.0	0.03398637209030784	2.780071336630498E-4
32-33	0.0	0.0	0.0	0.06401114252591722	2.780071336630498E-4
34-35	0.0	0.0	0.0	0.09945705206795608	2.780071336630498E-4
36-37	0.0	0.0	0.0	0.15297342529809316	2.780071336630498E-4
38-39	0.0	0.0	0.0	0.2230312229811817	4.1701070049457467E-4
40-41	0.0	0.0	0.0	0.3056688434625232	5.560142673260996E-4
42-43	0.0	0.0	0.0	0.37836770891541077	5.560142673260996E-4
44-45	0.0	0.0	0.0	0.46003230442893167	5.560142673260996E-4
46-47	0.0	0.0	0.0	0.5440599605785885	5.560142673260996E-4
48-49	0.0	0.0	0.0	0.6367753396552156	5.560142673260996E-4
50-51	0.0	0.0	0.0	0.7284481919806063	5.560142673260996E-4
52-53	0.0	0.0	0.0	0.816854460485456	5.560142673260996E-4
54-55	0.0	0.0	0.0	0.904913220073227	5.560142673260996E-4
56-57	0.0	0.0	0.0	1.004717781058262	5.560142673260996E-4
58-59	0.0	0.0	0.0	1.1110555096843786	5.560142673260996E-4
60-61	0.0	0.0	0.0	1.2271234879887016	5.560142673260996E-4
62-63	0.0	0.0	0.0	1.336797302218775	5.560142673260996E-4
64-65	0.0	0.0	0.0	1.4445250665132066	5.560142673260996E-4
66-67	0.0	0.0	0.0	1.5642766393385654	5.560142673260996E-4
68-69	0.0	0.0	0.0	1.7026546901193484	5.560142673260996E-4
70-71	0.0	0.0	0.0	1.8545855886662053	5.560142673260996E-4
72-73	0.0	0.0	0.0	1.991921112695752	5.560142673260996E-4
74-75	0.0	0.0	0.0	2.1277275974901517	5.560142673260996E-4
76-77	0.0	0.0	0.0	2.283272588774628	5.560142673260996E-4
78-79	0.0	0.0	0.0	2.4513279010739417	5.560142673260996E-4
80-81	0.0	0.0	0.0	2.632658054005666	6.255160507418621E-4
82-83	0.0	0.0	0.0	2.829765111772768	6.950178341576245E-4
84-85	0.0	0.0	0.0	3.01130377005474	6.950178341576245E-4
86-87	0.0	0.0	0.0	3.1958310050235887	6.950178341576245E-4
88-89	0.0	0.0	0.0	3.4028073160357293	6.950178341576245E-4
90-91	0.0	0.0	0.0	3.6175678267904354	6.950178341576245E-4
92-93	0.0	0.0	0.0	3.8348304017481087	6.950178341576245E-4
94-95	0.0	0.0	0.0	4.0491739018023205	6.950178341576245E-4
96-97	0.0	0.0	0.0	4.265463451792173	8.340214009891493E-4
98-99	0.0	0.0	0.0	4.507955174129767	8.340214009891493E-4
100-101	0.0	0.0	0.0	4.776788072381937	8.340214009891493E-4
102-103	0.0	0.0	0.0	5.05410018821083	0.0011815303180679616
104-105	0.0	0.0	0.0	5.3407255430174345	0.001390035668315249
106-107	0.0	0.0	0.0	5.628115417441611	0.001390035668315249
108-109	0.0	0.0	0.0	5.927112089696221	0.001390035668315249
110-111	0.0	0.0	0.0	6.23910559544958	0.001390035668315249
112-113	0.0	0.0	0.0	6.5676405256558885	0.001390035668315249
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGTTAG	35	0.0077919792	51.005123	2
CCGCAGA	425	0.0	42.024666	1
CTCAACG	315	0.0	41.579964	1
GGTATCA	3250	0.0	39.384655	1
GTATCAA	4885	0.0	36.92751	1
CATGGGT	1605	0.0	34.106976	4
GTCGACG	90	2.3926483E-4	33.05428	9
ATCAACG	5985	0.0	30.722383	3
ATGGGTA	630	0.0	30.221058	5
TATCAAC	6205	0.0	30.112614	2
CAACGCA	6210	0.0	29.892569	5
AACGCAG	6465	0.0	29.541784	6
TCAACGC	6310	0.0	29.326582	4
ACGCAGA	7015	0.0	27.055977	7
CGCAGAG	7280	0.0	26.234566	8
GAGTACT	2935	0.0	25.441097	12-13
ATGGGGG	1455	0.0	24.944056	5
GCAGAGT	7705	0.0	24.710274	9
CATGGGG	2605	0.0	24.668709	4
ACATGGG	6410	0.0	24.043756	3
>>END_MODULE