FastQCFastQC Report
Sun 1 Jan 2017
ERR1142916_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1142916_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences104235
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA3740.35880462416654674No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT3060.2935674197726292No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2380.22833021537871154No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG2320.22257399146160117No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT2050.1966709838346045No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA1860.17844294143042166No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG1510.1448649685806111No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG1510.1448649685806111No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC1460.1400681153163525No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC1450.13910874466350073No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC1390.13335252074639037No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA1390.13335252074639037No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT1380.13239315009353864No Hit
ATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGT1330.12759629682928No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC1290.12375881421787308No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC1250.11992133160646616No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC1230.1180025903007627No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG1210.11608384899505925No Hit
GTACATGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA1180.11320573703650406No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA1160.11128699573080059No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC1160.11128699573080059No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC1130.1084088837722454No Hit
GTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTAGGGCTGAA1130.1084088837722454No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1090.10457140116083849No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATA304.236874E-579.3676451
GTACAAA401.964061E-674.4071661
GGTAACA250.002048056671.430881
TTAAGAT150.004232494659.49711672-73
GTACAAG853.4400819E-642.0181661
GTAAGTA600.001300265239.6838232
TACAAGG758.116616E-539.683822
TACACTG700.002778056333.9983527
TACAAAA902.3720933E-433.0698552
ATGGGGG4000.031.2510095
TGGGGGG2500.030.93856
CATGGGG4450.030.7661084
TGCGACC400.00535350229.74855838-39
GAGTACT953.6889105E-828.18284412-13
GGGGGCT850.007182423527.9986448
CGCATTT557.731979E-427.04414434-35
GTATCTG900.00946992226.4558831
ATGGGAC907.2451985E-726.44316118-19
GGTGTTA450.00951361926.44316188-89
TCTCACT450.00951361926.44316156-57
Produced by FastQC (version 0.11.5)