FastQCFastQC Report
Sun 1 Jan 2017
ERR1142915_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1142915_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences426069
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA6730.15795563629365195No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5980.140352853645771No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT5310.12462770114699732No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAACG1150.001001625225.8790472
CTAACGC1200.001283784224.8007533
GGTATCA23900.024.1801321
CATGGGT4250.023.8087254
GTATCAA29900.022.117531
CATGGGG19300.021.2798184
ACGGTGC1400.003156481521.24536
ACATGGG44500.020.8660743
GTACATG45300.020.7799131
TACATGG46450.020.630812
TACGGGC1501.3809404E-719.83360970-71
ACGCCTA1500.004707137619.8289476
ACAGCGT1500.00471033919.8266168
CAACGCA32400.019.6568935
ATCAACG32300.019.3491953
AACGCAG32950.019.136896
ATGGGTA2501.2546214E-519.046985
TATCAAC33750.019.046982
ATGGGGG12350.018.7963625
CGCCTAC1600.00683911718.5874547
Produced by FastQC (version 0.11.5)