##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1142915_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	426069
Sequences flagged as poor quality	0
Sequence length	125
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.22478753441344	33.0	33.0	33.0	27.0	33.0
2	31.298301448826365	33.0	33.0	33.0	27.0	33.0
3	31.37923904344132	33.0	33.0	33.0	27.0	33.0
4	31.3380274087061	33.0	33.0	33.0	27.0	33.0
5	31.394598058061018	33.0	33.0	33.0	27.0	33.0
6	34.70106250396063	37.0	37.0	37.0	27.0	37.0
7	34.71288687982463	37.0	37.0	37.0	27.0	37.0
8	34.67995559404697	37.0	37.0	37.0	27.0	37.0
9	34.604237811246534	37.0	37.0	37.0	27.0	37.0
10-11	34.678749216676174	37.0	37.0	37.0	27.0	37.0
12-13	34.611841039831575	37.0	37.0	37.0	27.0	37.0
14-15	34.61466804672483	37.0	37.0	37.0	27.0	37.0
16-17	34.634498168137085	37.0	37.0	37.0	27.0	37.0
18-19	34.635099009784795	37.0	37.0	37.0	27.0	37.0
20-21	34.62995899725162	37.0	37.0	37.0	27.0	37.0
22-23	34.70017415019633	37.0	37.0	37.0	27.0	37.0
24-25	34.79914403535578	37.0	37.0	37.0	27.0	37.0
26-27	34.83953772745729	37.0	37.0	37.0	27.0	37.0
28-29	34.906393095953945	37.0	37.0	37.0	27.0	37.0
30-31	34.92192461784359	37.0	37.0	37.0	27.0	37.0
32-33	34.97687698471374	37.0	37.0	37.0	27.0	37.0
34-35	34.98017809321964	37.0	37.0	37.0	27.0	37.0
36-37	34.96395419521251	37.0	37.0	37.0	27.0	37.0
38-39	34.94439046257766	37.0	37.0	37.0	27.0	37.0
40-41	34.90572771076985	37.0	37.0	37.0	27.0	37.0
42-43	34.900320135940426	37.0	37.0	37.0	27.0	37.0
44-45	34.87335032588618	37.0	37.0	37.0	27.0	37.0
46-47	34.86070096627542	37.0	37.0	37.0	27.0	37.0
48-49	34.81939075595737	37.0	37.0	37.0	27.0	37.0
50-51	34.79445348053954	37.0	37.0	37.0	27.0	37.0
52-53	34.754246377934095	37.0	37.0	37.0	27.0	37.0
54-55	34.73360652852003	37.0	37.0	37.0	27.0	37.0
56-57	34.7120137818053	37.0	37.0	37.0	27.0	37.0
58-59	34.64633779974605	37.0	37.0	37.0	27.0	37.0
60-61	34.62703928236976	37.0	37.0	37.0	27.0	37.0
62-63	34.59689862440121	37.0	37.0	37.0	27.0	37.0
64-65	34.55233189929331	37.0	37.0	37.0	27.0	37.0
66-67	34.52311245361666	37.0	37.0	37.0	27.0	37.0
68-69	34.453458242679005	37.0	37.0	37.0	27.0	37.0
70-71	34.377826126754115	37.0	37.0	37.0	27.0	37.0
72-73	34.32959098174239	37.0	37.0	37.0	27.0	37.0
74-75	34.282338072002425	37.0	37.0	37.0	27.0	37.0
76-77	34.211414113676426	37.0	37.0	37.0	27.0	37.0
78-79	34.16565744046152	37.0	37.0	37.0	27.0	37.0
80-81	34.10122890893259	37.0	37.0	37.0	27.0	37.0
82-83	34.02036759304244	37.0	37.0	37.0	27.0	37.0
84-85	33.94823021623259	37.0	37.0	37.0	27.0	37.0
86-87	33.86293065207748	37.0	37.0	37.0	22.0	37.0
88-89	33.79406504580244	37.0	37.0	37.0	22.0	37.0
90-91	33.69436640544137	37.0	33.0	37.0	22.0	37.0
92-93	33.63873339764217	37.0	33.0	37.0	22.0	37.0
94-95	33.58466586397978	37.0	33.0	37.0	22.0	37.0
96-97	33.48176821125217	37.0	33.0	37.0	22.0	37.0
98-99	33.35846775991682	37.0	33.0	37.0	22.0	37.0
100-101	33.24663845527368	37.0	33.0	37.0	14.0	37.0
102-103	33.18281545946783	37.0	33.0	37.0	14.0	37.0
104-105	33.047383170331564	37.0	33.0	37.0	14.0	37.0
106-107	32.97343974802203	37.0	33.0	37.0	14.0	37.0
108-109	32.83980294271585	37.0	33.0	37.0	14.0	37.0
110-111	32.70675289683126	37.0	33.0	37.0	14.0	37.0
112-113	32.573229922852875	37.0	33.0	37.0	14.0	37.0
114-115	32.45081782528182	37.0	33.0	37.0	14.0	37.0
116-117	32.36507349748515	37.0	33.0	37.0	14.0	37.0
118-119	32.23070324290197	37.0	33.0	37.0	14.0	37.0
120-121	32.13695082251935	37.0	33.0	37.0	14.0	37.0
122-123	31.959257772802058	37.0	33.0	37.0	14.0	37.0
124-125	29.992847402650746	35.0	20.5	37.0	14.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	237.0
1	25.0
2	17.0
3	22.0
4	24.0
5	11.0
6	14.0
7	21.0
8	16.0
9	21.0
10	10.0
11	9.0
12	14.0
13	9.0
14	31.0
15	115.0
16	235.0
17	457.0
18	934.0
19	1840.0
20	2936.0
21	3999.0
22	4289.0
23	4787.0
24	4856.0
25	5240.0
26	6008.0
27	7181.0
28	8542.0
29	10095.0
30	12067.0
31	14585.0
32	18166.0
33	23555.0
34	34877.0
35	64885.0
36	195939.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.52608149218319	20.150815744036084	12.210202379693905	24.112900384086824
2	17.4448248233666	23.488542031348757	35.46742356067566	23.59920958460899
3	19.660510221829448	27.87934057565658	28.113203999417102	24.34694520309687
4	14.665021156558533	20.28937470615891	33.337094499294786	31.708509637987774
5	15.328348733864711	37.82605628835437	30.912275751804568	15.933319225976348
6	28.953099634203927	36.448982372717296	18.232244089585972	16.3656739034928
7	25.777143045057148	33.89762429355971	21.380418391171787	18.944814270211356
8	25.424628450106155	34.46395355391466	20.146365293200308	19.965052702778873
9	26.240909859631323	19.107615987372455	19.767884322678846	34.883589830317376
10-11	25.163445898732245	27.816278236769016	24.89462774734147	22.12564811715727
12-13	26.038390439033098	26.792857461636572	24.588413810598713	22.580338288731618
14-15	24.57625426505911	26.82820547454472	23.06132876044609	25.534211499950082
16-17	23.926586453307273	28.515755300706136	23.99811603531142	23.559542210675176
18-19	23.93832824726962	27.448769360518522	25.667120066342004	22.945782325869857
20-21	25.9068501646149	26.16184763343321	25.014652664318326	22.916649537633564
22-23	25.8932084562196	25.92668285183058	24.498441972428843	23.681666719520976
24-25	24.63692379597977	26.64504317456664	24.88517902208109	23.8328540073725
26-27	24.26246298660647	26.582575838549367	24.9706065791931	24.184354595651065
28-29	24.106200012451005	26.482932140856093	25.397228124702675	24.013639721990234
30-31	24.51314242353408	26.17654555769992	25.361817491986038	23.948494526779957
32-33	24.07527266960725	26.626532206436	25.046957318469882	24.25123780548687
34-35	24.207306256850277	26.622127876528793	25.187754847910643	23.982811018710287
36-37	24.226620057134266	26.593157983761838	25.001292098932492	24.1789298601714
38-39	24.32858137893205	26.600921574546405	24.998678598821428	24.071818447700117
40-41	23.974130791055035	26.626605811760975	25.258662719321435	24.140600677862558
42-43	24.175946822621064	26.55497192750376	25.218253525362755	24.050827724512416
44-45	24.290138541647085	26.625679079647348	24.97626745869128	24.107914920014288
46-47	24.131554678367724	26.614035396878542	25.027696030920872	24.22671389383286
48-49	24.200325872566676	26.68254913639071	25.16772334429365	23.949401646748967
50-51	23.777008380219723	27.00185979080584	25.152995109090888	24.068136719883547
52-53	23.672964872063908	26.761126039697373	25.027464812728162	24.53844427551055
54-55	23.802838907339034	26.57995425513044	24.994038130122657	24.62316870740787
56-57	24.076719396469695	26.386359951976956	24.81774033254313	24.719180319010217
58-59	24.00661220720599	26.297034728186354	25.246107339103546	24.45024572550411
60-61	24.011366466339943	26.530995822741392	25.255498804148125	24.202138906770543
62-63	23.595909562994326	26.523809971380096	25.251187796361652	24.62909266926392
64-65	23.565187153587882	26.238272797118512	25.28552934238381	24.9110107069098
66-67	24.03522415692982	26.10516027320337	25.101910872920957	24.757704696945844
68-69	23.908645780958512	25.943099211029608	25.057093449995065	25.09116155801681
70-71	23.80479643973669	26.04206195450279	25.165859349391955	24.987282256368566
72-73	24.134722586087186	26.08620055662529	25.334086778563464	24.44499007872406
74-75	24.14909278469219	25.923576083776574	25.457719877667216	24.46961125386402
76-77	23.886316180332702	25.702592279680903	25.598835289597154	24.812256250389236
78-79	23.872652865557267	25.904985483338443	25.553554202782074	24.668807448322212
80-81	23.667683052211665	25.99549970330942	25.665673781365484	24.671143463113427
82-83	23.914811115680628	25.81058692203748	25.755596028435463	24.519005933846426
84-85	23.89720701378337	26.04115620956739	25.28670796154429	24.774928815104946
86-87	23.664951857657535	26.116368866463457	25.455513279390097	24.76316599648891
88-89	24.037924871717443	25.57590555677627	25.529609858868625	24.85655971263767
90-91	24.095730055063466	25.69651875753509	25.661736884531233	24.54601430287021
92-93	23.785926310668433	26.08481065315437	25.56317554625384	24.56608748992336
94-95	23.122714496554707	26.159907686812	25.91431888472132	24.803058931911973
96-97	23.455931259298257	26.042756114815397	25.606545972459532	24.89476665342681
98-99	23.25463038056216	26.541207389802935	25.336685496136553	24.867476733498357
100-101	23.600100112450253	26.736721881268043	25.074643701904602	24.5885343043771
102-103	23.474790775066452	26.72203694955805	25.40041222188536	24.402760053490137
104-105	23.559586149839706	26.866415335583405	25.34973252982598	24.224265984750907
106-107	23.337787288951255	26.790249245236673	25.434436950673913	24.43752651513816
108-109	23.29950479342106	26.95514937282451	25.18408664117383	24.561259192580597
110-111	23.1910148272272	27.44012352497409	25.072561521594544	24.29630012620417
112-113	23.12802691716016	27.386424133307163	24.866877343822534	24.618671605710144
114-115	23.12400544376306	27.35020837201868	25.092080923089217	24.43370526112904
116-117	23.258432115899655	27.25258918634973	25.101328761150864	24.387649936599747
118-119	23.010101674837948	27.07639009669216	25.319525987486895	24.593982240983
120-121	23.099247888836537	27.030059771746423	25.50844759132904	24.362244748087996
122-123	22.974762446934633	27.32691555248492	25.560792082465312	24.137529918115135
124-125	23.056517998599038	27.265867773645937	25.11706023665913	24.5605539910959
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	389.0
1	318.0
2	174.0
3	80.5
4	56.5
5	58.5
6	56.5
7	56.5
8	66.0
9	70.5
10	71.0
11	65.5
12	72.5
13	85.5
14	105.5
15	121.0
16	135.0
17	170.5
18	215.5
19	265.0
20	329.0
21	402.5
22	497.5
23	605.5
24	728.5
25	895.5
26	1101.5
27	1349.5
28	1622.5
29	2084.5
30	2883.5
31	3716.0
32	4448.0
33	5262.0
34	6027.5
35	6791.0
36	7568.0
37	8655.5
38	9340.5
39	9422.0
40	9533.0
41	9746.0
42	10262.0
43	11142.5
44	12394.0
45	13810.5
46	15319.0
47	16650.0
48	18087.0
49	19000.5
50	19641.0
51	19343.5
52	17956.0
53	17569.5
54	17236.5
55	16509.5
56	15823.5
57	14214.0
58	12681.5
59	11385.0
60	9712.5
61	8195.0
62	6782.5
63	5415.0
64	4150.0
65	3211.0
66	2485.5
67	1929.0
68	1638.5
69	1427.5
70	1170.5
71	982.0
72	865.5
73	823.5
74	689.5
75	569.5
76	467.5
77	334.0
78	231.0
79	157.0
80	104.0
81	76.0
82	71.0
83	45.0
84	28.0
85	15.5
86	9.0
87	6.0
88	1.5
89	1.0
90	0.0
91	0.0
92	0.0
93	0.5
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.5
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0901262471571506
2	0.11031077126005412
3	0.1424651875635167
4	0.15701682121909832
5	0.17814016039655547
6	0.0990449903654103
7	0.08050339264297567
8	0.0668905740619477
9	0.07815635495659154
10-11	0.07428374277405772
12-13	0.0793298737997836
14-15	0.08719245004917045
16-17	0.08707509816485123
18-19	0.09294269238081157
20-21	0.08883537642963933
22-23	0.08695774628053203
24-25	0.06982437116992787
26-27	0.08883537642963933
28-29	0.09399885933968441
30-31	0.09517237818287648
32-33	0.09775411963789903
34-35	0.10502993646568982
36-37	0.09505502629855728
38-39	0.09024359904146981
40-41	0.10948930806981969
42-43	0.11136693821892699
44-45	0.11570895793873762
46-47	0.10948930806981969
48-49	0.10385641762249775
50-51	0.1134792721366727
52-53	0.12310212665084765
54-55	0.10502993646568982
56-57	0.10326965820090174
58-59	0.11559160605441841
60-61	0.10115732428315602
62-63	0.11523955040146079
64-65	0.10702491849911633
66-67	0.10608610342456269
68-69	0.10585139965592427
70-71	0.1132445683680343
72-73	0.10995871560709651
74-75	0.11934686635263303
76-77	0.13037794347863843
78-79	0.12286742288220923
80-81	0.12709209071770064
82-83	0.1276788501392967
84-85	0.13906198291825972
86-87	0.1314341104375113
88-89	0.12756149825497748
90-91	0.1314341104375113
92-93	0.13612818581027955
94-95	0.1316688142061497
96-97	0.13753640842211004
98-99	0.15772093252501357
100-101	0.12850031332953113
102-103	0.1337811481238954
104-105	0.14105696495168624
106-107	0.14187842814192067
108-109	0.1384752234966637
110-111	0.1333117405866186
112-113	0.14446016959694322
114-115	0.14739396670492338
116-117	0.14023550176145178
118-119	0.13964874233985575
120-121	0.12521446056859334
122-123	0.12497975679995493
124-125	0.15185333830905323
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	426069.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	70.15784512560998
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.78146163268437	59.48084654748714
2	10.200350701302202	14.312692494577343
3	2.420595212874023	5.0947123216982595
4	0.8456928816907759	2.3732796086996903
5	0.44426494638180336	1.558433565149599
6	0.23859570397085691	1.004361626809396
7	0.16138619524610104	0.7925755384041188
8	0.11321200140273335	0.6354168048618645
9	0.08834303459733917	0.5578161245285872
>10	0.6252350104287283	8.612993952791806
>50	0.060258246684799024	2.896852902617057
>100	0.01943814409083008	2.1885272280987342
>500	0.0011662886454498048	0.49149128427641064
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	673	0.15795563629365195	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	598	0.140352853645771	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	531	0.12462770114699732	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	2.3470376863841301E-4
16-17	0.0	0.0	0.0	3.520556529576195E-4	4.6940753727682603E-4
18-19	0.0	0.0	0.0	4.6940753727682603E-4	4.6940753727682603E-4
20-21	0.0	0.0	0.0	8.214631902344455E-4	4.6940753727682603E-4
22-23	0.0	0.0	0.0	0.0018776301491073041	5.867594215960326E-4
24-25	0.0	0.0	0.0	0.006454353637556358	7.041113059152391E-4
26-27	0.0	0.0	0.0	0.013143411043751128	7.041113059152391E-4
28-29	0.0	0.0	0.0	0.02464389570703337	7.041113059152391E-4
30-31	0.0	0.0	0.0	0.042598734007871966	7.041113059152391E-4
32-33	0.0	0.0	0.0	0.07475315031133455	7.041113059152391E-4
34-35	0.0	0.0	0.0	0.11230575329348064	7.041113059152391E-4
36-37	0.0	0.0	0.0	0.1712163992217223	7.041113059152391E-4
38-39	0.0	0.0	0.0	0.24174488169756542	7.041113059152391E-4
40-41	0.0	0.0	0.0	0.32835057232513987	7.041113059152391E-4
42-43	0.0	0.0	0.0	0.4046292971326241	7.041113059152391E-4
44-45	0.0	0.0	0.0	0.4757445390300632	7.041113059152391E-4
46-47	0.0	0.0	0.0	0.5684525276422363	7.041113059152391E-4
48-49	0.0	0.0	0.0	0.6677322217762851	7.041113059152391E-4
50-51	0.0	0.0	0.0	0.7643128225709921	7.041113059152391E-4
52-53	0.0	0.0	0.0	0.8477500123219478	7.041113059152391E-4
54-55	0.0	0.0	0.0	0.9331821841063301	7.041113059152391E-4
56-57	0.0	0.0	0.0	1.0323445263560598	8.214631902344455E-4
58-59	0.0	0.0	0.0	1.1386653335492607	0.0010561669588728586
60-61	0.0	0.0	0.0	1.2391185465265016	0.0011735188431920651
62-63	0.0	0.0	0.0	1.3359338510898469	0.0011735188431920651
64-65	0.0	0.0	0.0	1.4316929886943195	0.0011735188431920651
66-67	0.0	0.0	0.0	1.538835259077755	0.0011735188431920651
68-69	0.0	0.0	0.0	1.6628762008031561	0.0011735188431920651
70-71	0.0	0.0	0.0	1.7959532376211365	0.0011735188431920651
72-73	0.0	0.0	0.0	1.9273873480586476	0.0011735188431920651
74-75	0.0	0.0	0.0	2.0496680115192607	0.0011735188431920651
76-77	0.0	0.0	0.0	2.1846226784863485	0.0011735188431920651
78-79	0.0	0.0	0.0	2.33377692345606	0.0011735188431920651
80-81	1.1735188431920651E-4	0.0	0.0	2.495253116279288	0.0011735188431920651
82-83	2.3470376863841301E-4	0.0	0.0	2.6649439410048608	0.0014082226118304782
84-85	2.3470376863841301E-4	0.0	0.0	2.8261854300594504	0.0014082226118304782
86-87	2.3470376863841301E-4	0.0	0.0	2.9816766767823992	0.0014082226118304782
88-89	2.3470376863841301E-4	0.0	0.0	3.1545360023845905	0.0014082226118304782
90-91	2.3470376863841301E-4	0.0	0.0	3.3410081465678094	0.0014082226118304782
92-93	2.3470376863841301E-4	0.0	0.0	3.5279496982883054	0.0014082226118304782
94-95	2.3470376863841301E-4	0.0	0.0	3.710901285941948	0.0014082226118304782
96-97	2.3470376863841301E-4	0.0	0.0	3.897842837662444	0.0015255744961496847
98-99	2.3470376863841301E-4	0.0	0.0	4.109545636974293	0.0016429263804688913
100-101	2.3470376863841301E-4	0.0	0.0	4.34225442357928	0.0016429263804688913
102-103	2.3470376863841301E-4	0.0	0.0	4.574611154531308	0.0016429263804688913
104-105	2.3470376863841301E-4	0.0	0.0	4.816238684344555	0.0016429263804688913
106-107	2.3470376863841301E-4	0.0	0.0	5.04906482283386	0.0016429263804688913
108-109	2.3470376863841301E-4	0.0	0.0	5.302779596731985	0.0016429263804688913
110-111	2.3470376863841301E-4	0.0	0.0	5.589587602008125	0.0016429263804688913
112-113	2.3470376863841301E-4	0.0	0.0	5.877099718590181	0.0016429263804688913
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTAACG	115	0.0010016252	25.879047	2
CTAACGC	120	0.0012837842	24.800753	3
GGTATCA	2390	0.0	24.180132	1
CATGGGT	425	0.0	23.808725	4
GTATCAA	2990	0.0	22.11753	1
CATGGGG	1930	0.0	21.279818	4
ACGGTGC	140	0.0031564815	21.2453	6
ACATGGG	4450	0.0	20.866074	3
GTACATG	4530	0.0	20.779913	1
TACATGG	4645	0.0	20.63081	2
TACGGGC	150	1.3809404E-7	19.833609	70-71
ACGCCTA	150	0.0047071376	19.828947	6
ACAGCGT	150	0.004710339	19.826616	8
CAACGCA	3240	0.0	19.656893	5
ATCAACG	3230	0.0	19.349195	3
AACGCAG	3295	0.0	19.13689	6
ATGGGTA	250	1.2546214E-5	19.04698	5
TATCAAC	3375	0.0	19.04698	2
ATGGGGG	1235	0.0	18.796362	5
CGCCTAC	160	0.006839117	18.587454	7
>>END_MODULE