FastQCFastQC Report
Sun 1 Jan 2017
ERR1142915_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1142915_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences426069
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17060.4004046292971326No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16880.3961799614616412No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12960.3041760841553833No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA6460.1516186345404148No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT6100.14316929886943194No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5720.13425055566117225No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA30450.031.4779911
GGTATCA22500.029.899531
CATTTCG450.00952797426.444235102-103
TATCAAC39300.024.983652
ATCAACG39600.024.4938433
CAACGCA40300.024.2132075
TCAACGC42200.023.1257554
AACGCAG43050.023.081126
ACGCAGA46500.021.1127367
CGCAGAG47200.020.7996238
CCGTCGT1500.004701341519.8331789
GCAGAGT49200.019.8331789
AAGACTA1805.775744E-419.8331765
CTAAGGT1600.00682146318.5957853
AGAGTAC48850.018.57457210-11
TAAGACT1959.887136E-418.3096964
GTGTAAG1650.00812410118.0407471
CATGGGG17800.017.5490216-17
TTAAGAC1700.0096745617.5019173
GAGTACT18950.017.42598312-13
Produced by FastQC (version 0.11.5)