##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1142915_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	426069
Sequences flagged as poor quality	0
Sequence length	125
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.233262687498975	33.0	14.0	33.0	14.0	33.0
2	27.70350577019215	33.0	27.0	33.0	14.0	33.0
3	29.893885732123202	33.0	27.0	33.0	27.0	33.0
4	30.748902173122193	33.0	27.0	33.0	27.0	33.0
5	31.664533209409743	33.0	33.0	33.0	27.0	33.0
6	35.0700191752979	37.0	37.0	37.0	33.0	37.0
7	35.902081587724055	37.0	37.0	37.0	33.0	37.0
8	36.05760569297461	37.0	37.0	37.0	33.0	37.0
9	36.122567002058354	37.0	37.0	37.0	37.0	37.0
10-11	36.16338081390573	37.0	37.0	37.0	37.0	37.0
12-13	36.19444737824155	37.0	37.0	37.0	37.0	37.0
14-15	36.21452276509204	37.0	37.0	37.0	37.0	37.0
16-17	36.2251912248955	37.0	37.0	37.0	37.0	37.0
18-19	36.217118588773175	37.0	37.0	37.0	37.0	37.0
20-21	36.13844823256327	37.0	37.0	37.0	37.0	37.0
22-23	36.18474003037066	37.0	37.0	37.0	37.0	37.0
24-25	36.21092827687534	37.0	37.0	37.0	37.0	37.0
26-27	36.14039627384297	37.0	37.0	37.0	37.0	37.0
28-29	36.139493837852555	37.0	37.0	37.0	37.0	37.0
30-31	36.095950421175914	37.0	37.0	37.0	37.0	37.0
32-33	36.03816165926176	37.0	37.0	37.0	37.0	37.0
34-35	35.975135482750446	37.0	37.0	37.0	37.0	37.0
36-37	35.90648815098024	37.0	37.0	37.0	37.0	37.0
38-39	35.84123698274223	37.0	37.0	37.0	35.0	37.0
40-41	35.75948848660663	37.0	37.0	37.0	33.0	37.0
42-43	35.668235661360015	37.0	37.0	37.0	33.0	37.0
44-45	35.56768856687532	37.0	37.0	37.0	33.0	37.0
46-47	35.43207438231836	37.0	37.0	37.0	33.0	37.0
48-49	35.20120097918412	37.0	37.0	37.0	33.0	37.0
50-51	35.13812786191908	37.0	37.0	37.0	33.0	37.0
52-53	35.09486139568943	37.0	37.0	37.0	33.0	37.0
54-55	35.03502601691275	37.0	37.0	37.0	33.0	37.0
56-57	35.00555191764714	37.0	37.0	37.0	33.0	37.0
58-59	34.97098592012092	37.0	37.0	37.0	30.0	37.0
60-61	34.93569820850614	37.0	37.0	37.0	27.0	37.0
62-63	34.921033917041605	37.0	37.0	37.0	27.0	37.0
64-65	34.900417538004405	37.0	37.0	37.0	27.0	37.0
66-67	34.8831726786037	37.0	37.0	37.0	27.0	37.0
68-69	34.81336121614105	37.0	37.0	37.0	27.0	37.0
70-71	34.80129392187651	37.0	37.0	37.0	27.0	37.0
72-73	34.787818405000124	37.0	37.0	37.0	27.0	37.0
74-75	34.73386235562785	37.0	37.0	37.0	27.0	37.0
76-77	34.68224512931005	37.0	37.0	37.0	27.0	37.0
78-79	34.59569342054925	37.0	37.0	37.0	27.0	37.0
80-81	34.51940648110987	37.0	37.0	37.0	27.0	37.0
82-83	34.53129774754793	37.0	37.0	37.0	27.0	37.0
84-85	34.486343761221775	37.0	37.0	37.0	27.0	37.0
86-87	34.41255172284301	37.0	37.0	37.0	27.0	37.0
88-89	34.35968469895721	37.0	37.0	37.0	27.0	37.0
90-91	34.29019830121412	37.0	37.0	37.0	27.0	37.0
92-93	34.189452882044925	37.0	37.0	37.0	27.0	37.0
94-95	34.14679312505721	37.0	37.0	37.0	27.0	37.0
96-97	34.08791299061889	37.0	37.0	37.0	24.5	37.0
98-99	33.99468161260265	37.0	37.0	37.0	22.0	37.0
100-101	33.922356472777885	37.0	37.0	37.0	22.0	37.0
102-103	33.84277194538913	37.0	37.0	37.0	22.0	37.0
104-105	33.70704627654206	37.0	33.0	37.0	22.0	37.0
106-107	33.63093067085378	37.0	33.0	37.0	22.0	37.0
108-109	33.55577617709808	37.0	33.0	37.0	22.0	37.0
110-111	33.50971556250279	37.0	33.0	37.0	22.0	37.0
112-113	33.44916668426945	37.0	33.0	37.0	22.0	37.0
114-115	33.31239893068963	37.0	33.0	37.0	14.0	37.0
116-117	33.262922202741805	37.0	33.0	37.0	14.0	37.0
118-119	33.09457740412938	37.0	33.0	37.0	14.0	37.0
120-121	33.004730454456904	37.0	33.0	37.0	14.0	37.0
122-123	32.92434089314172	37.0	33.0	37.0	14.0	37.0
124-125	31.30041260922527	35.0	27.5	37.0	14.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	9.0
15	216.0
16	81.0
17	14.0
18	74.0
19	356.0
20	1137.0
21	2576.0
22	4131.0
23	5539.0
24	4721.0
25	3528.0
26	3156.0
27	3555.0
28	4474.0
29	5509.0
30	7395.0
31	10272.0
32	14729.0
33	22393.0
34	37618.0
35	81802.0
36	212784.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	41.68106727274435	21.441485015543265	12.480160034561456	24.397287677150935
2	16.68012317398325	24.01329377746066	34.65535694168012	24.651226106875963
3	21.55871466828143	26.17885835392859	28.141451267282996	24.120975710506983
4	15.91783947197942	18.35632000450658	33.30735792547249	32.418482598041514
5	16.071164521012545	36.15167055896539	31.16381687810259	16.61334804191947
6	29.945619136806478	34.552854115178526	18.416500613750355	17.085026134264638
7	25.815536920076326	31.802830058042243	22.776592523746153	19.605040498135278
8	26.24082014884913	31.603566558468227	21.238109320321357	20.917503972361285
9	26.428817867528497	16.018062802034414	21.17098404249077	36.38213528794632
10-11	25.7365591019295	25.2159861430895	26.319328559458675	22.72812619552232
12-13	26.78191498724961	24.15626235872184	26.5662204520197	22.495602202008847
14-15	24.79378315928073	24.38974014741761	25.475598407298357	25.340878286003303
16-17	23.591014600921447	26.17299075971263	26.51601031757767	23.719984321788253
18-19	23.523067859900625	24.783544449373224	29.174265201176336	22.519122489549815
20-21	24.647938562932158	23.987588660805233	28.786420755664672	22.57805202059794
22-23	24.508941040066283	23.847663171927554	28.60733824803025	23.036057539975918
24-25	23.673769235553603	24.571399065638815	28.618419894546975	23.136411804260604
26-27	23.28946689010623	24.654630972666205	28.8488168499716	23.207085287255964
28-29	23.13392901149814	24.425386498431006	29.27131520950832	23.169369280562538
30-31	23.550418072465895	24.18166348833798	29.243479536452988	23.024438902743142
32-33	23.295991963742964	24.27975280999087	28.979109017553494	23.44514620871267
34-35	23.244943894064107	24.33467349185226	29.0463516472684	23.374030966815234
36-37	23.503228365491815	24.360957314225224	28.677766642335595	23.458047677947366
38-39	23.4025474747048	24.36260324032023	28.74733904602306	23.487510238951906
40-41	23.173098814025156	24.341470627583245	28.99090040674736	23.49453015164424
42-43	23.61163430677064	24.3288915488743	28.52235854647444	23.537115597880618
44-45	23.592774879186234	24.539335178104952	28.096622847473064	23.77126709523575
46-47	23.443855337985163	24.594021156197705	28.012716250184827	23.949407255632305
48-49	23.85376546991215	24.631339055411214	27.881751547284594	23.63314392739204
50-51	23.50487831783115	24.892799053674405	27.649746871985524	23.952575756508924
52-53	23.412335178117942	24.797801279613957	27.424037590420262	24.365825951847835
54-55	23.386822322206026	24.891508182946893	27.34545343594582	24.376216058901257
56-57	23.65872663817363	24.66443228678923	27.201227970117515	24.475613104919624
58-59	23.552640534749067	24.698816388894755	27.54190049029617	24.206642586060003
60-61	23.64663939408875	24.752328848144316	27.689177105116773	23.91185465265016
62-63	23.13486782657269	24.888926441491872	27.661716764186085	24.314488967749355
64-65	23.079243033405387	24.666427268822655	27.779420704158248	24.474908993613713
66-67	23.47944051249975	24.50462777698891	27.769947790085396	24.24598392042594
68-69	23.45887637917802	24.30627433584701	27.814626269454006	24.42022301552096
70-71	23.205865716585812	24.415176884495235	28.27476300786962	24.10419439104934
72-73	23.620117868232608	24.379971319199473	28.36618012575428	23.63373068681364
74-75	23.561089870420048	24.478898957680563	28.352919362826206	23.60709180907318
76-77	23.367631457889352	24.56368467005267	28.369649915037037	23.699033957020944
78-79	23.31828882176361	24.5060072429583	28.41429439832515	23.761409536952936
80-81	23.00930131035114	24.789177339820544	28.496675421117235	23.704845928711077
82-83	23.325681990475722	24.532646120698757	28.668595931644873	23.473075957180644
84-85	23.392220508884712	24.6624373047558	28.130654893925634	23.814687292433856
86-87	23.07560512499149	24.74434892001061	28.22218936369461	23.957856591303287
88-89	23.311247708704457	24.145619606214016	28.36125134661287	24.181881338468653
90-91	23.552405830980426	24.268487029096224	28.20892860076654	23.9701785391568
92-93	23.3524382201005	24.512931004133133	28.189448187969557	23.94518258779681
94-95	22.733524382201008	24.65375326531618	28.226179327761464	24.386543024721348
96-97	23.064456695981168	24.601883732447092	27.824131772083867	24.509527799487877
98-99	23.0165137959199	24.75344311236704	27.571772580902582	24.658270510810482
100-101	23.292408723490148	24.877953753860886	27.35760488936038	24.47203263328858
102-103	23.294493725774142	24.884965680401155	27.5301976090699	24.290342984754798
104-105	23.382652534337243	24.99859177408301	27.296581766290828	24.32217392528892
106-107	23.285457620077523	25.052544367865732	27.298427365552726	24.363570646504023
108-109	23.248636367903718	25.060788418750057	27.171132307518985	24.519442905827237
110-111	23.2070664693588	25.504232300674655	26.985566874810036	24.303134355156505
112-113	23.12253706576665	25.480968955586796	26.720797433267542	24.675696545379015
114-115	23.235782165715722	25.3092823429179	26.906566338158083	24.5483691532083
116-117	23.247490127737976	25.301507390260937	26.860767366673123	24.59023511532797
118-119	23.067516772695214	25.202404800212175	27.063507989987436	24.666570437105182
120-121	23.10243378072151	25.27651264887676	27.32138603102105	24.299667539380682
122-123	22.90590186135537	25.578409639006665	27.193086427866465	24.3226020717715
124-125	22.964805839484587	25.346954103247153	27.2044171663948	24.48382289087346
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	67.0
1	65.5
2	143.0
3	252.5
4	249.5
5	254.0
6	366.5
7	651.0
8	901.5
9	1054.0
10	1099.5
11	1002.5
12	973.5
13	944.5
14	912.0
15	944.5
16	939.5
17	905.5
18	895.0
19	882.0
20	854.5
21	791.5
22	795.5
23	861.0
24	881.0
25	927.5
26	1053.0
27	1191.5
28	1384.5
29	1814.5
30	2506.0
31	3141.0
32	3878.0
33	4689.5
34	5418.0
35	6128.0
36	6972.5
37	8210.0
38	8862.5
39	8854.5
40	9024.0
41	9211.0
42	9576.5
43	10548.5
44	11843.5
45	13205.0
46	14845.0
47	16399.5
48	17820.0
49	18774.0
50	19630.0
51	19296.5
52	17827.5
53	17228.5
54	16787.0
55	16269.0
56	15880.5
57	14308.5
58	12608.0
59	11620.5
60	9954.0
61	8108.0
62	6630.5
63	5260.0
64	3991.5
65	3091.0
66	2379.5
67	1847.5
68	1551.0
69	1303.0
70	1108.5
71	980.0
72	853.5
73	799.0
74	680.0
75	596.5
76	471.5
77	294.0
78	208.0
79	158.0
80	133.0
81	100.5
82	61.5
83	42.5
84	34.0
85	15.5
86	8.0
87	8.0
88	6.5
89	4.0
90	2.5
91	1.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0377873067507845
2	0.0011735188431920651
3	0.0
4	0.005867594215960325
5	0.003285852760937782
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	8.214631902344455E-4
14-15	1.1735188431920651E-4
16-17	0.0
18-19	0.0
20-21	0.001642926380468891
22-23	0.0
24-25	5.867594215960326E-4
26-27	7.041113059152391E-4
28-29	0.0
30-31	0.0015255744961496847
32-33	0.0
34-35	0.0
36-37	4.6940753727682603E-4
38-39	0.0
40-41	0.0014082226118304782
42-43	3.5205565295761955E-4
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	7.041113059152391E-4
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	1.1735188431920651E-4
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	2.3470376863841301E-4
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	2.3470376863841301E-4
100-101	2.3470376863841301E-4
102-103	1.1735188431920651E-4
104-105	2.3470376863841301E-4
106-107	1.1735188431920651E-4
108-109	2.3470376863841301E-4
110-111	1.1735188431920651E-4
112-113	4.6940753727682603E-4
114-115	4.6940753727682603E-4
116-117	3.5205565295761955E-4
118-119	0.0010561669588728586
120-121	8.214631902344455E-4
122-123	0.001994982033426511
124-125	9.388150745536521E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	426069.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	67.88840560816564
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.17707557278125	57.14647449394186
2	10.524142222321291	14.289344717339395
3	2.5489854115538177	5.191396665265879
4	0.8924733979127344	2.4235438412799013
5	0.4657583902585584	1.5809797256639662
6	0.26146858863817246	1.0650411359557719
7	0.1559004033124585	0.7408680870186957
8	0.11902961274258608	0.6464584503401254
9	0.09168633509079142	0.5601995194832855
>10	0.6789016305425613	9.27267488952886
>50	0.06154768732991298	2.883617454615412
>100	0.020648256393523384	2.4423272074255635
>500	0.0011912455611648106	0.49277860211632785
>1k	0.0011912455611648106	1.264295210024933
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1706	0.4004046292971326	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1688	0.3961799614616412	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1296	0.3041760841553833	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	646	0.1516186345404148	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	610	0.14316929886943194	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	572	0.13425055566117225	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	3.520556529576195E-4	0.0
18-19	0.0	0.0	0.0	4.6940753727682603E-4	0.0
20-21	0.0	0.0	0.0	8.214631902344455E-4	0.0
22-23	0.0	0.0	0.0	0.001642926380468891	0.0
24-25	0.0	0.0	0.0	0.006102297984598739	0.0
26-27	0.0	0.0	0.0	0.012908707275112716	0.0
28-29	0.0	0.0	0.0	0.024174488169756543	0.0
30-31	0.0	0.0	0.0	0.04236403023923355	0.0
32-33	0.0	0.0	0.0	0.07416639088973852	0.0
34-35	0.0	0.0	0.0	0.11148429010324619	0.0
36-37	0.0	0.0	0.0	0.1692214171882958	0.0
38-39	0.0	0.0	0.0	0.2392804921268621	0.0
40-41	0.0	0.0	0.0	0.3258861827544365	0.0
42-43	0.0	0.0	0.0	0.40099138871872864	0.0
44-45	0.0	0.0	0.0	0.471637223078891	0.0
46-47	0.0	0.0	0.0	0.5627022853105952	0.0
48-49	0.0	0.0	0.0	0.6599869974112175	0.0
50-51	0.0	0.0	0.0	0.7556287831313707	0.0
52-53	0.0	0.0	0.0	0.8397700841882418	0.0
54-55	0.0	0.0	0.0	0.9256716635099009	0.0
56-57	0.0	0.0	0.0	1.0243645982223537	0.0
58-59	0.0	0.0	0.0	1.130802757299874	1.1735188431920651E-4
60-61	0.0	0.0	0.0	1.2304345070868803	2.3470376863841301E-4
62-63	0.0	0.0	0.0	1.3278365710718218	2.3470376863841301E-4
64-65	0.0	0.0	0.0	1.4245345237508478	2.3470376863841301E-4
66-67	0.0	0.0	0.0	1.5322635535558793	2.3470376863841301E-4
68-69	0.0	0.0	0.0	1.6556003839753655	2.3470376863841301E-4
70-71	1.1735188431920651E-4	0.0	0.0	1.7892641802149418	2.3470376863841301E-4
72-73	2.3470376863841301E-4	0.0	0.0	1.9202288831151761	2.3470376863841301E-4
74-75	2.3470376863841301E-4	0.0	0.0	2.04239219469147	2.3470376863841301E-4
76-77	3.520556529576195E-4	0.0	0.0	2.177464213542877	2.3470376863841301E-4
78-79	4.6940753727682603E-4	0.0	0.0	2.3276746254714613	2.3470376863841301E-4
80-81	4.6940753727682603E-4	0.0	0.0	2.490206985253562	2.3470376863841301E-4
82-83	4.6940753727682603E-4	0.0	0.0	2.660719273169369	2.3470376863841301E-4
84-85	4.6940753727682603E-4	0.0	0.0	2.8233689848357892	2.3470376863841301E-4
86-87	4.6940753727682603E-4	0.0	0.0	2.9797990466332918	2.3470376863841301E-4
88-89	4.6940753727682603E-4	0.0	0.0	3.1545360023845905	2.3470376863841301E-4
90-91	4.6940753727682603E-4	0.0	0.0	3.3426510729482786	2.3470376863841301E-4
92-93	4.6940753727682603E-4	0.0	0.0	3.5325264217767547	2.3470376863841301E-4
94-95	4.6940753727682603E-4	0.0	0.0	3.7182944546540586	2.3470376863841301E-4
96-97	4.6940753727682603E-4	0.0	0.0	3.9071136365236616	2.3470376863841301E-4
98-99	4.6940753727682603E-4	0.0	0.0	4.120107306563021	2.3470376863841301E-4
100-101	4.6940753727682603E-4	0.0	0.0	4.353402852589604	2.3470376863841301E-4
102-103	4.6940753727682603E-4	0.0	0.0	4.5872851580377825	2.3470376863841301E-4
104-105	4.6940753727682603E-4	0.0	0.0	4.830790318000136	2.3470376863841301E-4
106-107	4.6940753727682603E-4	0.0	0.0	5.065024679101272	2.3470376863841301E-4
108-109	4.6940753727682603E-4	0.0	0.0	5.3212038425701005	2.3470376863841301E-4
110-111	4.6940753727682603E-4	0.0	0.0	5.612236515681732	3.520556529576195E-4
112-113	4.6940753727682603E-4	0.0	0.0	5.904560059520875	4.6940753727682603E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	3045	0.0	31.477991	1
GGTATCA	2250	0.0	29.89953	1
CATTTCG	45	0.009527974	26.444235	102-103
TATCAAC	3930	0.0	24.98365	2
ATCAACG	3960	0.0	24.493843	3
CAACGCA	4030	0.0	24.213207	5
TCAACGC	4220	0.0	23.125755	4
AACGCAG	4305	0.0	23.08112	6
ACGCAGA	4650	0.0	21.112736	7
CGCAGAG	4720	0.0	20.799623	8
CCGTCGT	150	0.0047013415	19.833178	9
GCAGAGT	4920	0.0	19.833178	9
AAGACTA	180	5.775744E-4	19.833176	5
CTAAGGT	160	0.006821463	18.595785	3
AGAGTAC	4885	0.0	18.574572	10-11
TAAGACT	195	9.887136E-4	18.309696	4
GTGTAAG	165	0.008124101	18.040747	1
CATGGGG	1780	0.0	17.54902	16-17
TTAAGAC	170	0.00967456	17.501917	3
GAGTACT	1895	0.0	17.425983	12-13
>>END_MODULE