FastQCFastQC Report
Sun 1 Jan 2017
ERR1142914_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1142914_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences901848
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19360.21467032138453487No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22300.036.857081
GTATCAA30850.033.399321
ATCAACG39300.026.0505683
CAACGCA39750.025.7542255
AACGCAG40200.025.4503866
TATCAAC40550.025.3943182
TCAACGC40300.025.255054
ACGCAGA44950.022.6261257
CGCAGAG46900.021.6877888
GTACATG60600.019.951251
GCAGAGT50000.019.8672839
TACATGG61800.019.1666372
ACATGGG60850.019.0745963
CATGGGG32550.019.0168914
AGAGTAC47250.018.5687310-11
CAGAGTA50000.018.49917610-11
GAGTACA51900.017.13617912-13
GGGTATA2150.001892993316.621091
GTGTATA3256.42789E-616.4932351
AGTACAT52400.016.2914912-13
Produced by FastQC (version 0.11.5)