##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1142914_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	901848
Sequences flagged as poor quality	0
Sequence length	125
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.40340611721709	33.0	33.0	33.0	27.0	33.0
2	31.49272826462996	33.0	33.0	33.0	27.0	33.0
3	31.56357723252699	33.0	33.0	33.0	27.0	33.0
4	31.55885914256061	33.0	33.0	33.0	27.0	33.0
5	31.54929766435142	33.0	33.0	33.0	27.0	33.0
6	34.90234274511891	37.0	37.0	37.0	27.0	37.0
7	34.88951685871677	37.0	37.0	37.0	27.0	37.0
8	34.89662781311263	37.0	37.0	37.0	27.0	37.0
9	34.92282845889773	37.0	37.0	37.0	27.0	37.0
10-11	34.93259451703614	37.0	37.0	37.0	27.0	37.0
12-13	34.89658734066051	37.0	37.0	37.0	27.0	37.0
14-15	34.919761977628156	37.0	37.0	37.0	27.0	37.0
16-17	34.91496072508893	37.0	37.0	37.0	27.0	37.0
18-19	34.91410082408565	37.0	37.0	37.0	27.0	37.0
20-21	34.91651420194978	37.0	37.0	37.0	27.0	37.0
22-23	34.95654034826268	37.0	37.0	37.0	27.0	37.0
24-25	35.00896381651897	37.0	37.0	37.0	27.0	37.0
26-27	35.0324899539612	37.0	37.0	37.0	30.0	37.0
28-29	35.07210139624416	37.0	37.0	37.0	33.0	37.0
30-31	35.05273449627875	37.0	37.0	37.0	30.0	37.0
32-33	35.090657738332844	37.0	37.0	37.0	33.0	37.0
34-35	35.08504093816253	37.0	37.0	37.0	33.0	37.0
36-37	35.06091492136147	37.0	37.0	37.0	33.0	37.0
38-39	35.052615851008156	37.0	37.0	37.0	30.0	37.0
40-41	35.01000002217668	37.0	37.0	37.0	30.0	37.0
42-43	35.00171425783502	37.0	37.0	37.0	27.0	37.0
44-45	34.97598985638378	37.0	37.0	37.0	27.0	37.0
46-47	34.97801347898981	37.0	37.0	37.0	27.0	37.0
48-49	34.9355434618694	37.0	37.0	37.0	27.0	37.0
50-51	34.908217349265065	37.0	37.0	37.0	27.0	37.0
52-53	34.877369024491934	37.0	37.0	37.0	27.0	37.0
54-55	34.85816345991786	37.0	37.0	37.0	27.0	37.0
56-57	34.823095466198296	37.0	37.0	37.0	27.0	37.0
58-59	34.77260469613505	37.0	37.0	37.0	27.0	37.0
60-61	34.74530353230256	37.0	37.0	37.0	27.0	37.0
62-63	34.7206319690236	37.0	37.0	37.0	27.0	37.0
64-65	34.674714031632824	37.0	37.0	37.0	27.0	37.0
66-67	34.659170946767084	37.0	37.0	37.0	27.0	37.0
68-69	34.605727905367644	37.0	37.0	37.0	27.0	37.0
70-71	34.548945609459686	37.0	37.0	37.0	27.0	37.0
72-73	34.49711093221917	37.0	37.0	37.0	27.0	37.0
74-75	34.45389799611465	37.0	37.0	37.0	27.0	37.0
76-77	34.38782311431638	37.0	37.0	37.0	27.0	37.0
78-79	34.349411430751076	37.0	37.0	37.0	27.0	37.0
80-81	34.28255093984795	37.0	37.0	37.0	27.0	37.0
82-83	34.202324005819165	37.0	37.0	37.0	27.0	37.0
84-85	34.136008506976786	37.0	37.0	37.0	27.0	37.0
86-87	34.057145439142744	37.0	37.0	37.0	27.0	37.0
88-89	33.99302598664076	37.0	37.0	37.0	27.0	37.0
90-91	33.910313046100896	37.0	37.0	37.0	24.5	37.0
92-93	33.85457250002217	37.0	35.0	37.0	22.0	37.0
94-95	33.79349347118361	37.0	33.0	37.0	22.0	37.0
96-97	33.6797985913369	37.0	33.0	37.0	22.0	37.0
98-99	33.57433957828813	37.0	33.0	37.0	22.0	37.0
100-101	33.46442360575175	37.0	33.0	37.0	22.0	37.0
102-103	33.404651338141235	37.0	33.0	37.0	22.0	37.0
104-105	33.23953038649529	37.0	33.0	37.0	18.0	37.0
106-107	33.186891249966735	37.0	33.0	37.0	18.0	37.0
108-109	33.075460055353005	37.0	33.0	37.0	14.0	37.0
110-111	32.92567816306074	37.0	33.0	37.0	14.0	37.0
112-113	32.80755404458401	37.0	33.0	37.0	14.0	37.0
114-115	32.68519362464628	37.0	33.0	37.0	14.0	37.0
116-117	32.587691052150696	37.0	33.0	37.0	14.0	37.0
118-119	32.47110876777461	37.0	33.0	37.0	14.0	37.0
120-121	32.3901644179507	37.0	33.0	37.0	14.0	37.0
122-123	32.1870021333972	37.0	33.0	37.0	14.0	37.0
124-125	30.22710312602567	35.0	27.5	37.0	14.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	503.0
1	53.0
2	38.0
3	40.0
4	23.0
5	25.0
6	40.0
7	28.0
8	34.0
9	36.0
10	31.0
11	13.0
12	26.0
13	26.0
14	49.0
15	230.0
16	422.0
17	730.0
18	1530.0
19	2962.0
20	4549.0
21	6232.0
22	7297.0
23	8094.0
24	9019.0
25	10500.0
26	12259.0
27	14735.0
28	17361.0
29	20843.0
30	25210.0
31	31294.0
32	38471.0
33	51144.0
34	76813.0
35	141783.0
36	419405.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.75774767306235	19.293800640788746	12.134035160147246	24.81441652600166
2	17.557168864615246	22.552062519426315	35.59633231206429	24.294436303894145
3	20.08825168830765	26.501711070101685	27.908208472961537	25.501828768629125
4	15.028667438831137	18.83877716688192	33.67233056338763	32.460224830899314
5	15.85689310855749	36.71737377462142	31.144886231109787	16.280846885711302
6	30.55898704616918	35.14105368775174	18.159559318911082	16.140399947167992
7	26.901749024281706	32.28187688582459	21.509414280498618	19.30695980939508
8	25.720797493187042	33.442629135486854	20.604755310555422	20.231818060770685
9	26.50937858223224	17.918790344327075	19.785407296684564	35.78642377675612
10-11	25.37872567078719	26.676302181584145	25.826360993364254	22.118611154264407
12-13	26.166704397155566	25.29374295327634	25.32758942125869	23.211963228309408
14-15	24.529080823253338	25.738125268080037	23.32802675889637	26.404767149770247
16-17	24.464203274264	27.609789498916864	24.008032511807947	23.917974715011187
18-19	24.45401471276155	26.783285583724403	25.322830084084814	23.439869619429235
20-21	25.799916321056354	25.713684836687335	24.89148927433487	23.594909567921444
22-23	25.642553701508835	25.583791666111782	24.669678567782203	24.103976064597177
24-25	24.89608461475299	26.04697663374071	24.69036211155175	24.366576639954552
26-27	24.51387971762241	26.15527438148054	24.90919058933726	24.421655311559793
28-29	24.485460599334075	26.03312985571587	25.031964483906773	24.449445061043285
30-31	24.827925679654026	25.852186174897323	25.053565988136402	24.26632215731225
32-33	24.43200515872113	25.98683571615269	24.88544653514961	24.695712589976566
34-35	24.74654193740302	25.982420543922004	24.952438458584275	24.318599060090705
36-37	24.734996254265976	26.12741044921062	24.71052412530174	24.427069171221664
38-39	24.568474230534036	25.972230083447368	24.923228200082793	24.536067485935806
40-41	24.41738493387132	26.063747717547546	25.13444963064508	24.384417717936053
42-43	24.511472243497952	25.994760620289277	25.035243708859213	24.45852342735356
44-45	24.498114790995178	26.045135117283703	24.962354099587213	24.494395992133907
46-47	24.425140152526353	25.949848678006887	24.977924693651357	24.6470864758154
48-49	24.522227420580283	26.11864133049847	25.007561668528638	24.351569580392606
50-51	24.203793021151892	26.289011117093015	24.993089974635488	24.5141058871196
52-53	24.244065899968863	26.076902539383507	25.11197122080131	24.567060339846325
54-55	24.274485993653105	25.901058148789062	25.231570640006307	24.592885217551526
56-57	24.394054342945847	25.83162999423371	24.87322772723413	24.90108793558631
58-59	24.379681803851568	25.88457507981546	25.110564946915503	24.62517816941747
60-61	24.43996024154758	25.938227885193303	25.169420178136072	24.45239169512304
62-63	24.103710043476163	26.067880091714553	25.143019531247834	24.685390333561454
64-65	24.196190911734735	25.700467755506175	25.242535780965213	24.860805551793877
66-67	24.360687017605006	25.770874622672558	25.0201879547146	24.848250405007835
68-69	24.346990696032663	25.723438405338626	24.928294405853663	25.001276492775048
70-71	24.179837443859075	25.630343516958277	25.205803800376973	24.98401523880567
72-73	24.350243540731466	25.721353438209032	25.178326428942494	24.750076592117008
74-75	24.286661064232977	25.622586170721775	25.378469558691783	24.712283206353472
76-77	24.254290401103585	25.433092502796427	25.32872951945842	24.983887576641568
78-79	24.28890840320928	25.58231188448016	25.319978729144072	24.808800983166492
80-81	24.123240138307658	25.64903182974547	25.420554418534426	24.807173613412445
82-83	24.277619327284214	25.6455546957658	25.335808343611955	24.741017633338032
84-85	24.225234197953206	25.77037996862186	25.12044386854935	24.883941964875582
86-87	23.9730323855625	25.82046385629115	25.324355230873426	24.88214852727293
88-89	24.188710980108077	25.41264567842641	25.315725637460208	25.082917704005308
90-91	24.383748867532375	25.46980752491429	25.253694953191335	24.892748654362
92-93	24.193357667272704	25.814719720205957	25.226763632326204	24.765158980195135
94-95	23.851414252494294	25.74604018738644	25.475752063243757	24.926793496875504
96-97	23.9786460707249	25.681841776399978	25.36223234722786	24.977279805647264
98-99	23.83924634996574	25.842753403496616	25.261946248130148	25.056053998407503
100-101	24.011717175754306	26.033135382562346	25.061477980613482	24.893669461069866
102-103	24.078672500098538	25.887991224315666	25.343285918657266	24.690050356928534
104-105	23.855049773202975	26.04346063947323	25.369287740746294	24.732201846577503
106-107	23.932111736930203	25.973312162203428	25.366492800612928	24.72808330025344
108-109	23.922009270018005	25.93956223619615	25.304517704246564	24.833910789539278
110-111	23.79851102133988	26.314985991210015	25.181903319086764	24.70459966836334
112-113	23.772664334119114	26.396780329317814	25.054047961944903	24.776507374618166
114-115	23.690503355946902	26.30624868485413	25.201109747153318	24.802138212045655
116-117	23.78239466684728	26.356591299095466	25.198513707896858	24.662500326160394
118-119	23.60719718419329	26.102394990173543	25.347923120482328	24.94248470515084
120-121	23.61923941805917	26.15436452845396	25.468931533858935	24.75746451962793
122-123	23.619226830303987	26.349161414230565	25.380548362655386	24.65106339281006
124-125	23.682069932399603	26.2160158798462	25.12491497897031	24.976999208783887
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1207.0
1	942.5
2	469.0
3	224.0
4	170.5
5	141.0
6	139.5
7	147.0
8	150.0
9	162.0
10	160.0
11	149.5
12	146.5
13	158.0
14	196.5
15	219.0
16	227.0
17	270.0
18	340.5
19	408.5
20	485.0
21	619.0
22	736.0
23	921.0
24	1182.0
25	1450.5
26	1893.5
27	2444.0
28	3111.5
29	4126.0
30	5659.5
31	7130.0
32	8784.5
33	10475.0
34	11994.0
35	13504.5
36	14985.5
37	16809.5
38	18138.0
39	19134.0
40	19832.5
41	20487.0
42	21957.5
43	24262.0
44	26567.0
45	28372.5
46	30799.5
47	33115.0
48	35596.0
49	37810.0
50	38253.5
51	37717.5
52	37192.0
53	37404.5
54	37014.5
55	35749.0
56	34159.5
57	31623.0
58	29093.0
59	26345.5
60	23137.0
61	19902.5
62	16621.0
63	13227.0
64	10239.5
65	8275.5
66	6629.0
67	5166.0
68	4185.0
69	3543.0
70	3037.0
71	2731.5
72	2472.5
73	2252.0
74	1834.5
75	1567.5
76	1294.0
77	913.5
78	693.5
79	480.5
80	386.5
81	298.0
82	197.5
83	163.0
84	108.0
85	51.0
86	34.5
87	22.5
88	10.0
89	4.0
90	4.0
91	2.5
92	0.5
93	0.5
94	0.0
95	1.0
96	1.5
97	0.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.08549112489022541
2	0.11177049791095617
3	0.13771722064028527
4	0.14958174770027766
5	0.1678775137273687
6	0.09746653538068499
7	0.0795034196449956
8	0.06919126061154429
9	0.07750751789658568
10-11	0.07407013155210192
12-13	0.07983606993639727
14-15	0.08477038259218848
16-17	0.084271407155086
18-19	0.08798600207573783
20-21	0.08704349291676645
22-23	0.08382787343321713
24-25	0.07030009491621649
26-27	0.08704349291676645
28-29	0.09402914903620122
30-31	0.09497165819517259
32-33	0.09419547418190205
34-35	0.10090392172516875
36-37	0.09125706327452077
38-39	0.08881762780424196
40-41	0.10567190923525915
42-43	0.1084439949969396
44-45	0.11304565736132918
46-47	0.10583823438095998
48-49	0.102289964606009
50-51	0.1089984121492757
52-53	0.11737011114955069
54-55	0.10367600748684923
56-57	0.10217908117554177
58-59	0.1131010990765628
60-61	0.10062671314900072
62-63	0.11193682305665699
64-65	0.1053392589438575
66-67	0.10417498292395172
68-69	0.10445219150011975
70-71	0.10977459616254624
72-73	0.1077786944141363
74-75	0.11631671856011212
76-77	0.1270169696001987
78-79	0.120308522056932
80-81	0.1222489820901083
82-83	0.12319149124907966
84-85	0.1353886686004737
86-87	0.12618534387169456
88-89	0.12258163238150997
90-91	0.12862477934197336
92-93	0.13145230681888745
94-95	0.12668431930879703
96-97	0.13500057659383843
98-99	0.15268648375335978
100-101	0.12407855869281742
102-103	0.1305652393751497
104-105	0.14004577268009688
106-107	0.13865972979925664
108-109	0.13333732513683014
110-111	0.12901287134860864
112-113	0.139602238958228
114-115	0.14220799957420763
116-117	0.13500057659383843
118-119	0.13505601830907205
120-121	0.12235986552057554
122-123	0.11925512946749342
124-125	0.1480848213889702
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	901848.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	79.09152408331006
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.31611797604948	68.2687332368055
2	9.636828701331575	15.243829386362002
3	2.1415693348731093	5.081399478755845
4	0.8088025345649794	2.558777005647532
5	0.3487231291660506	1.3790521884421967
6	0.20555718224330705	0.9754698497936325
7	0.12838174017540396	0.7107735246458152
8	0.09209296918116056	0.5827018631916233
9	0.061330595850505	0.4365657268878575
>10	0.24354600856015465	3.146345485411328
>50	0.010546558330171816	0.5640127831419053
>100	0.006327505631497558	0.7832074761659761
>500	0.0	0.0
>1k	1.7576404261864025E-4	0.26913199474880106
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1936	0.21467032138453487	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	2.7720857616804603E-4	5.544171523360921E-5
22-23	0.0	0.0	0.0	0.0011642760199057933	1.6632514570082764E-4
24-25	0.0	0.0	0.0	0.002993852622614897	2.7720857616804603E-4
26-27	0.0	0.0	0.0	0.005377846377660093	3.326502914016553E-4
28-29	0.0	0.0	0.0	0.010644809324852969	3.326502914016553E-4
30-31	0.0	0.0	0.0	0.02334096211334948	3.326502914016553E-4
32-33	0.0	0.0	0.0	0.04707001623333422	3.326502914016553E-4
34-35	0.0	0.0	0.0	0.07717486760518402	3.326502914016553E-4
36-37	0.0	0.0	0.0	0.11770276144095235	3.326502914016553E-4
38-39	0.0	0.0	0.0	0.17563935386007398	3.326502914016553E-4
40-41	0.0	0.0	0.0	0.24277927100797475	3.326502914016553E-4
42-43	0.0	0.0	0.0	0.2986645199634528	4.989754371024829E-4
44-45	0.0	0.0	0.0	0.3599830570118246	7.76184013270529E-4
46-47	0.0	0.0	0.0	0.44325651329270566	7.76184013270529E-4
48-49	0.0	0.0	0.0	0.5210412397654594	7.76184013270529E-4
50-51	0.0	0.0	0.0	0.5986041993772786	7.76184013270529E-4
52-53	0.0	0.0	0.0	0.6679063434192902	7.76184013270529E-4
54-55	0.0	0.0	0.0	0.7439169350045685	7.76184013270529E-4
56-57	0.0	0.0	0.0	0.8245846306694697	7.76184013270529E-4
58-59	0.0	0.0	0.0	0.9139012339108142	7.76184013270529E-4
60-61	0.0	0.0	0.0	1.006876990357577	7.76184013270529E-4
62-63	0.0	0.0	0.0	1.094973875863782	7.76184013270529E-4
64-65	0.0	0.0	0.0	1.1841241539594254	7.76184013270529E-4
66-67	0.0	0.0	0.0	1.284085566525623	7.76184013270529E-4
68-69	0.0	0.0	0.0	1.3910880769264886	7.76184013270529E-4
70-71	0.0	0.0	0.0	1.5169962122220153	7.76184013270529E-4
72-73	0.0	0.0	0.0	1.6368612005570784	7.76184013270529E-4
74-75	0.0	0.0	0.0	1.7511265756535468	7.76184013270529E-4
76-77	0.0	0.0	0.0	1.8719895148628152	7.76184013270529E-4
78-79	0.0	0.0	0.0	2.015140023595994	7.76184013270529E-4
80-81	0.0	0.0	0.0	2.1611180598060873	8.316257285041382E-4
82-83	0.0	0.0	0.0	2.318295322493369	8.870674437377473E-4
84-85	0.0	0.0	0.0	2.4679325119088804	8.870674437377473E-4
86-87	0.0	0.0	0.0	2.6208407625231747	8.870674437377473E-4
88-89	0.0	0.0	0.0	2.787997533952506	8.870674437377473E-4
90-91	0.0	0.0	0.0	2.9650783724086542	0.001053392589438575
92-93	0.0	0.0	0.0	3.1442105543284455	0.0011088343046721841
94-95	0.0	0.0	0.0	3.3320470855399136	0.0011088343046721841
96-97	0.0	0.0	0.0	3.5203271504732507	0.0011088343046721841
98-99	0.0	0.0	0.0	3.7178105401353667	0.0011088343046721841
100-101	0.0	0.0	0.0	3.9386903336260657	0.0011088343046721841
102-103	0.0	0.0	0.0	4.169383310713114	0.0011088343046721841
104-105	0.0	0.0	0.0	4.4101112382574446	0.0011088343046721841
106-107	0.0	0.0	0.0	4.649286797775235	0.0011642760199057933
108-109	0.0	0.0	0.0	4.891400768200406	0.0012197177351394026
110-111	0.0	0.0	0.0	5.15197682979837	0.0013306011656066211
112-113	0.0	0.0	0.0	5.4307377739929565	0.0014414845960738394
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2230	0.0	36.85708	1
GTATCAA	3085	0.0	33.39932	1
ATCAACG	3930	0.0	26.050568	3
CAACGCA	3975	0.0	25.754225	5
AACGCAG	4020	0.0	25.450386	6
TATCAAC	4055	0.0	25.394318	2
TCAACGC	4030	0.0	25.25505	4
ACGCAGA	4495	0.0	22.626125	7
CGCAGAG	4690	0.0	21.687788	8
GTACATG	6060	0.0	19.95125	1
GCAGAGT	5000	0.0	19.867283	9
TACATGG	6180	0.0	19.166637	2
ACATGGG	6085	0.0	19.074596	3
CATGGGG	3255	0.0	19.016891	4
AGAGTAC	4725	0.0	18.56873	10-11
CAGAGTA	5000	0.0	18.499176	10-11
GAGTACA	5190	0.0	17.136179	12-13
GGGTATA	215	0.0018929933	16.62109	1
GTGTATA	325	6.42789E-6	16.493235	1
AGTACAT	5240	0.0	16.29149	12-13
>>END_MODULE