FastQCFastQC Report
Sun 1 Jan 2017
ERR1142914_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1142914_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences901848
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26450.29328667358579275No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23140.25658425810114344No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18970.21034586759631335No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26600.038.266141
GTATCAA38250.033.6141741
TCAACGC50200.026.6721784
TATAGCG450.00953138926.44386552-53
CAACGCA50250.026.405865
ATCAACG50050.026.2765223
TATCAAC50500.025.9259742
AACGCAG53600.024.8651286
ACGCAGA59350.022.355877
CGCAGAG61150.021.6005088
AGAGTAC61950.020.02498610-11
GCAGAGT66450.019.9672079
GTACATG55800.019.84171
ACATGGG55150.019.3146673
CATGGGG28100.019.0596924
TACATGG57650.018.9942552
GAGTACT27200.018.9214612-13
CAGAGTA65050.018.33895110-11
CCTAACC1650.00814874718.0339072
AGTACTT29100.017.68603912-13
Produced by FastQC (version 0.11.5)