FastQCFastQC Report
Sun 1 Jan 2017
ERR1142913_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1142913_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences444333
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6270.14111038342864474No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAACG1107.7333226E-427.0516552
CTAACGC1150.001002495325.8754983
GGTATCA21450.025.2767121
GTATCAA27050.024.22881
CGCCTAC1250.001636204423.7893627
AACGCAG29300.021.3105956
CATGGGG17800.020.7294714
CAACGCA30500.020.683435
ATCAACG31150.020.6339443
GTACTAG1450.003833420320.545111
TATCAAC32200.020.1459242
ACGCCTA1805.7977746E-419.8222356
ACGCAGA31550.019.793057
TCAACGC32000.019.7138944
CGCAGAG31500.019.6356648
GTACATG40950.019.2055381
AGTAGAG1550.00567525419.1979483
ACATGGG40000.018.8955823
TACATGG41350.018.8543832
ATTATAC1908.297892E-418.7937833
Produced by FastQC (version 0.11.5)