##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1142913_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	444333
Sequences flagged as poor quality	0
Sequence length	125
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.279002459866813	33.0	33.0	33.0	27.0	33.0
2	31.32971667645662	33.0	33.0	33.0	27.0	33.0
3	31.405139838814584	33.0	33.0	33.0	27.0	33.0
4	31.369515656050755	33.0	33.0	33.0	27.0	33.0
5	31.41546542795606	33.0	33.0	33.0	27.0	33.0
6	34.77161948358551	37.0	37.0	37.0	27.0	37.0
7	34.74253544076177	37.0	37.0	37.0	27.0	37.0
8	34.70020682686184	37.0	37.0	37.0	27.0	37.0
9	34.68993525126425	37.0	37.0	37.0	27.0	37.0
10-11	34.72717421393414	37.0	37.0	37.0	27.0	37.0
12-13	34.67264304024234	37.0	37.0	37.0	27.0	37.0
14-15	34.67357139802806	37.0	37.0	37.0	27.0	37.0
16-17	34.68913292508097	37.0	37.0	37.0	27.0	37.0
18-19	34.694404872021664	37.0	37.0	37.0	27.0	37.0
20-21	34.69742287878685	37.0	37.0	37.0	27.0	37.0
22-23	34.753027571663594	37.0	37.0	37.0	27.0	37.0
24-25	34.83729882768104	37.0	37.0	37.0	27.0	37.0
26-27	34.87446239644591	37.0	37.0	37.0	27.0	37.0
28-29	34.936126733778494	37.0	37.0	37.0	27.0	37.0
30-31	34.92809221912394	37.0	37.0	37.0	27.0	37.0
32-33	34.98033907002181	37.0	37.0	37.0	27.0	37.0
34-35	34.99195648308813	37.0	37.0	37.0	27.0	37.0
36-37	34.972204405254615	37.0	37.0	37.0	27.0	37.0
38-39	34.95376553170708	37.0	37.0	37.0	27.0	37.0
40-41	34.922769634485846	37.0	37.0	37.0	27.0	37.0
42-43	34.91129288169008	37.0	37.0	37.0	27.0	37.0
44-45	34.891492416723494	37.0	37.0	37.0	27.0	37.0
46-47	34.87975797431206	37.0	37.0	37.0	27.0	37.0
48-49	34.835327333328834	37.0	37.0	37.0	27.0	37.0
50-51	34.80965852187436	37.0	37.0	37.0	27.0	37.0
52-53	34.768685872982644	37.0	37.0	37.0	27.0	37.0
54-55	34.742482552500036	37.0	37.0	37.0	27.0	37.0
56-57	34.707991528875866	37.0	37.0	37.0	27.0	37.0
58-59	34.639646391332626	37.0	37.0	37.0	27.0	37.0
60-61	34.623400692723706	37.0	37.0	37.0	27.0	37.0
62-63	34.59140554494039	37.0	37.0	37.0	27.0	37.0
64-65	34.533752838524265	37.0	37.0	37.0	27.0	37.0
66-67	34.523241577825644	37.0	37.0	37.0	27.0	37.0
68-69	34.4565258488566	37.0	37.0	37.0	27.0	37.0
70-71	34.38400703976522	37.0	37.0	37.0	27.0	37.0
72-73	34.3217924394542	37.0	37.0	37.0	27.0	37.0
74-75	34.29661312574128	37.0	37.0	37.0	27.0	37.0
76-77	34.209088678986255	37.0	37.0	37.0	27.0	37.0
78-79	34.1531486520245	37.0	37.0	37.0	27.0	37.0
80-81	34.0947149997862	37.0	37.0	37.0	27.0	37.0
82-83	34.0076429164613	37.0	37.0	37.0	27.0	37.0
84-85	33.94548570554066	37.0	37.0	37.0	27.0	37.0
86-87	33.86361580166226	37.0	37.0	37.0	22.0	37.0
88-89	33.79402385148076	37.0	37.0	37.0	22.0	37.0
90-91	33.70583998937734	37.0	33.0	37.0	22.0	37.0
92-93	33.648218790861804	37.0	33.0	37.0	22.0	37.0
94-95	33.58353869732835	37.0	33.0	37.0	22.0	37.0
96-97	33.46834581271254	37.0	33.0	37.0	22.0	37.0
98-99	33.367421505942616	37.0	33.0	37.0	22.0	37.0
100-101	33.24714009537892	37.0	33.0	37.0	14.0	37.0
102-103	33.182365478143645	37.0	33.0	37.0	14.0	37.0
104-105	33.048145197408246	37.0	33.0	37.0	14.0	37.0
106-107	32.98180643796432	37.0	33.0	37.0	14.0	37.0
108-109	32.856137176396985	37.0	33.0	37.0	14.0	37.0
110-111	32.70976159772063	37.0	33.0	37.0	14.0	37.0
112-113	32.5879531792597	37.0	33.0	37.0	14.0	37.0
114-115	32.462059986541625	37.0	33.0	37.0	14.0	37.0
116-117	32.35379208836616	37.0	33.0	37.0	14.0	37.0
118-119	32.23025298593622	37.0	33.0	37.0	14.0	37.0
120-121	32.145847821341206	37.0	33.0	37.0	14.0	37.0
122-123	31.946017964004476	37.0	33.0	37.0	14.0	37.0
124-125	29.969611755147604	35.0	20.5	37.0	14.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	240.0
1	42.0
2	18.0
3	22.0
4	18.0
5	11.0
6	16.0
7	14.0
8	20.0
9	18.0
10	14.0
11	14.0
12	13.0
13	17.0
14	33.0
15	187.0
16	299.0
17	526.0
18	951.0
19	1798.0
20	3009.0
21	3926.0
22	4315.0
23	4625.0
24	4857.0
25	5426.0
26	6227.0
27	7274.0
28	9153.0
29	10402.0
30	12782.0
31	15283.0
32	18934.0
33	25008.0
34	37638.0
35	69548.0
36	201655.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.36654938936215	20.30849487118267	12.201945211527113	24.123010527928066
2	17.46535679682747	23.38928820891823	35.71505824564566	23.43029674860864
3	19.55750995670215	27.865017096001317	28.3002495092084	24.277223438088132
4	14.43052869334704	19.955277240533608	34.2002876322635	31.41390643385585
5	15.305202213093686	37.901709420979294	31.111822555699348	15.681265810227668
6	29.014576339919344	36.62671502917521	18.435803275733885	15.92290535517156
7	25.957984499266857	33.77366947535807	21.64922214789438	18.619123877480693
8	25.29261398826616	34.37586568021351	20.376563854824724	19.954956476695607
9	26.359260585666245	18.57658010734887	19.884542528487092	35.17961677849779
10-11	24.93403589933591	27.637128188264864	25.623238282714873	21.805597629684353
12-13	25.77333403908593	26.538249726051617	25.136242160233618	22.552174074628834
14-15	24.28373845838486	26.762018034090424	23.356241989291274	25.598001518233442
16-17	23.849469462411854	28.513424780345836	24.228802993220878	23.40830276402143
18-19	23.984614344927014	27.476009190845197	25.78358713281672	22.755789331411062
20-21	25.642192380079226	26.155000185840738	25.18141998092035	23.021387453159686
22-23	25.492830683391134	26.048873666628747	24.907729752072633	23.55056589790749
24-25	24.481458166998678	26.709901477333254	25.11185008428874	23.696790271379328
26-27	24.238147744768654	26.60921825179165	25.159905346396823	23.992728657042875
28-29	24.01158835217417	26.558142259188006	25.530982621977316	23.89928676666051
30-31	24.46231653882112	26.292479077259262	25.52777120715485	23.717433176764775
32-33	23.92249050448981	26.58337350808977	25.314376855735826	24.17975913168459
34-35	24.321161075057816	26.46246771255642	25.396376596911896	23.819994615473867
36-37	24.236618436495437	26.70005158668478	25.019091578755297	24.044238398064486
38-39	24.202600649880893	26.501174178216036	25.263472075958347	24.03275309594472
40-41	23.95422270637577	26.73875647890196	25.429401513367857	23.877619301354418
42-43	24.10675661060942	26.668762054110562	25.398123614340562	23.826357720939452
44-45	24.07328130069577	26.707996863621613	25.284008976531236	23.934712859151375
46-47	24.147825205312785	26.59377921975509	25.189879121745694	24.06851645318643
48-49	24.13066718034157	26.654207797269635	25.2628922825978	23.952232739790993
50-51	23.76833929856936	26.893920190887556	25.234581920884857	24.10315858965823
52-53	23.849843333299532	26.69305382326464	25.304404356697574	24.152698486738256
54-55	23.890895327014366	26.631467007923863	25.160809194117817	24.316828470943946
56-57	24.112659492619997	26.38101149326884	25.08727246921104	24.419056544900123
58-59	23.953637974680692	26.390596007539237	25.38510155775594	24.270664460024133
60-61	24.009480509532327	26.473669506914373	25.49272515083653	24.02412483271677
62-63	23.645433969236453	26.64765679482745	25.271825770130768	24.435083465805327
64-65	23.522824820823864	26.299407214810845	25.47230176844202	24.70546619592328
66-67	24.048782026441245	26.117376963492333	25.32567162026982	24.5081693897966
68-69	23.88321319179951	25.945952644071273	25.293820117764614	24.877014046364607
70-71	23.792625238129038	26.120234462045243	25.462429406217996	24.62471089360772
72-73	24.137103615158182	25.98551954028427	25.59585165358198	24.281525190975568
74-75	24.0234075729853	25.96250912597907	25.54800940989842	24.46607389113721
76-77	23.825298421862712	25.861012144197986	25.81526576841517	24.498423665524133
78-79	23.832352735720868	26.009797713716576	25.70795692431108	24.449892626251472
80-81	23.67550526958603	26.031214156787367	25.815788702782726	24.477491870843878
82-83	23.87673745695794	25.969573094881827	25.85081306675801	24.302876381402225
84-85	23.94412698985059	25.994291451488046	25.47504566500759	24.586535893653775
86-87	23.59181631441182	26.223896388960565	25.749853798369077	24.43443349825854
88-89	23.847730389135705	25.780620620575505	25.72541128140876	24.64623770888003
90-91	24.036738805616967	25.893285736500143	25.68031695163918	24.389658506243702
92-93	23.856491226488657	26.147790733837855	25.655703345788577	24.34001469388491
94-95	23.09534996659151	26.305043723992927	25.979296878080987	24.62030943133457
96-97	23.322537896715392	26.38030587821666	25.66474196878113	24.63241425628682
98-99	23.206675916230957	26.66511881106657	25.460721849221585	24.667483423480892
100-101	23.37150332217491	26.825393427310452	25.333259720051693	24.469843530462942
102-103	23.546168338877322	26.74691925611416	25.498958812234633	24.207953592773887
104-105	23.410081849807913	26.92230973554049	25.530874270445832	24.13673414420577
106-107	23.420543137975258	26.866825489484565	25.557664177641144	24.154967194899037
108-109	23.35381973320352	26.85983380999709	25.492383521632423	24.293962935166967
110-111	23.226079068797855	27.093430089412983	25.389681730341255	24.290809111447906
112-113	23.214549339439884	27.3143833401138	25.22682630249743	24.24424101794889
114-115	23.175731566859227	27.31483464098397	25.333186077070625	24.17624771508618
116-117	23.256980136599616	27.154723121529933	25.398265290829166	24.19003145104129
118-119	23.122413839732438	26.87307869139164	25.49480739585493	24.509700073020994
120-121	23.05768251407248	26.963274098978307	25.753086030276673	24.225957356672538
122-123	23.084558616415976	27.185510675454903	25.594163709086814	24.135766999042307
124-125	23.117054368406816	26.941439004598323	25.366964205211435	24.574542421783427
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	394.0
1	333.5
2	183.0
3	86.5
4	73.0
5	57.5
6	48.0
7	53.5
8	68.0
9	70.0
10	61.5
11	64.5
12	72.0
13	90.5
14	102.0
15	105.0
16	130.0
17	181.0
18	222.5
19	257.0
20	324.0
21	372.0
22	454.0
23	579.0
24	690.5
25	839.0
26	1069.5
27	1327.5
28	1654.0
29	2275.5
30	3339.0
31	4312.0
32	5337.0
33	6452.0
34	7645.5
35	8765.5
36	9345.0
37	10305.0
38	10787.0
39	10447.5
40	10057.5
41	9929.5
42	10380.5
43	11158.0
44	12363.5
45	13694.0
46	15006.5
47	16360.5
48	17796.5
49	18777.0
50	19133.0
51	18818.0
52	18075.0
53	17957.0
54	17755.0
55	17051.5
56	16310.0
57	14806.0
58	13300.5
59	12083.5
60	10561.0
61	8907.0
62	7266.0
63	5733.0
64	4314.0
65	3379.5
66	2689.5
67	2029.5
68	1647.0
69	1446.0
70	1196.0
71	1033.0
72	912.0
73	843.5
74	694.5
75	520.5
76	421.5
77	313.0
78	230.5
79	166.0
80	125.5
81	103.0
82	77.0
83	51.0
84	30.0
85	18.0
86	12.0
87	11.0
88	7.0
89	1.0
90	1.5
91	2.0
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.5
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.08349593660610398
2	0.11770451440698754
3	0.14898735858016396
4	0.1600151237922909
5	0.1780196384243349
6	0.10420112843295457
7	0.07966997724679464
8	0.07156794566237483
9	0.08102031584419794
10-11	0.07685677183553777
12-13	0.0815829569264493
14-15	0.08912234742861773
16-17	0.089234875645068
18-19	0.09294830678792707
20-21	0.0910353271082724
22-23	0.0883346499134658
24-25	0.07280575604332787
26-27	0.0896849885108691
28-29	0.09879977404334137
30-31	0.09857471761044082
32-33	0.09879977404334137
34-35	0.1054389388139076
36-37	0.09452370181823092
38-39	0.09126038354117295
40-41	0.10994006747191859
42-43	0.11275327288317546
44-45	0.11455372434637985
46-47	0.11207810358447381
48-49	0.10633916454550979
50-51	0.11511636542863124
52-53	0.12524390490915596
54-55	0.10892731352386611
56-57	0.10453871308230539
58-59	0.11736692975763673
60-61	0.10690180562776117
62-63	0.11894232478794058
64-65	0.1116279907186727
66-67	0.10825214422516445
68-69	0.10836467244161473
70-71	0.11444119612992958
72-73	0.11106534963642133
74-75	0.12265575593079965
76-77	0.13098284394812
78-79	0.1251313766927057
80-81	0.1260316024243079
82-83	0.13075778751521944
84-85	0.13784706515158676
86-87	0.13424616222517796
88-89	0.12771952567106204
90-91	0.1363841983377332
92-93	0.13750948050223594
94-95	0.1313204285974708
96-97	0.14111038342864474
98-99	0.15945248271003956
100-101	0.12884480783556476
102-103	0.13705936763643484
104-105	0.14729943533340986
106-107	0.1467367942511585
108-109	0.1404352141299431
110-111	0.1362716701212829
112-113	0.14774954819921096
114-115	0.14898735858016396
116-117	0.141222911645095
118-119	0.14066027056284364
120-121	0.12580654599140734
122-123	0.1255814895585068
124-125	0.15596410800008104
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	444333.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	71.79544715773288
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.2380484836525	61.19703805736347
2	9.937193272221018	14.268904689438456
3	2.310430132192813	4.976350934024487
4	0.8691500136331023	2.496040555037528
5	0.4145162422447824	1.4880189483053639
6	0.2444257768992341	1.052919476961406
7	0.16439724644931641	0.8262081674230081
8	0.11513787342046755	0.6613100085610333
9	0.09172077554808257	0.5926620684715775
>10	0.5433155179884188	7.620590786370782
>50	0.054212648704838586	2.6763020674555293
>100	0.01708069753389578	1.976501994741307
>500	3.7131951160643E-4	0.16715224584607494
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	627	0.14111038342864474	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	2.250564329005498E-4	0.0
5	0.0	0.0	0.0	2.250564329005498E-4	0.0
6	0.0	0.0	0.0	2.250564329005498E-4	0.0
7	0.0	0.0	0.0	2.250564329005498E-4	0.0
8	0.0	0.0	0.0	2.250564329005498E-4	0.0
9	0.0	0.0	0.0	2.250564329005498E-4	0.0
10-11	0.0	0.0	0.0	2.250564329005498E-4	0.0
12-13	0.0	0.0	0.0	2.250564329005498E-4	0.0
14-15	0.0	0.0	0.0	2.250564329005498E-4	0.0
16-17	0.0	0.0	0.0	7.876975151519243E-4	0.0
18-19	0.0	0.0	0.0	9.002257316021992E-4	0.0
20-21	0.0	0.0	0.0	0.001350338597403299	0.0
22-23	0.0	0.0	0.0	0.0033758464935082473	0.0
24-25	0.0	0.0	0.0	0.00686422120346677	0.0
26-27	0.0	0.0	0.0	0.012940744891781614	0.0
28-29	0.0	0.0	0.0	0.02183047399135333	0.0
30-31	0.0	0.0	0.0	0.03960993219049677	0.0
32-33	0.0	0.0	0.0	0.0704426634978721	0.0
34-35	0.0	0.0	0.0	0.10611410811260924	0.0
36-37	0.0	0.0	0.0	0.15562652335073018	0.0
38-39	0.0	0.0	0.0	0.22291839678799458	0.0
40-41	0.0	0.0	0.0	0.2949364553161705	0.0
42-43	0.0	0.0	0.0	0.36346613913438797	0.0
44-45	0.0	0.0	0.0	0.43154571008680426	0.0
46-47	0.0	0.0	0.0	0.5118908566323006	0.0
48-49	0.0	0.0	0.0	0.6006756194115674	0.0
50-51	0.0	0.0	0.0	0.6912608336540387	0.0
52-53	0.0	0.0	0.0	0.7664296822428225	0.0
54-55	0.0	0.0	0.0	0.8471124134376695	0.0
56-57	0.0	0.0	0.0	0.937810155896591	0.0
58-59	0.0	0.0	0.0	1.0345844220438276	0.0
60-61	0.0	0.0	0.0	1.135522232199724	0.0
62-63	0.0	0.0	0.0	1.236910155221422	0.0
64-65	0.0	0.0	0.0	1.3281705387625946	0.0
66-67	0.0	0.0	0.0	1.4351848726068062	0.0
68-69	0.0	0.0	0.0	1.55547753599215	0.0
70-71	0.0	0.0	0.0	1.6839847591783639	0.0
72-73	0.0	0.0	0.0	1.812942095230379	0.0
74-75	0.0	0.0	0.0	1.9368356615421316	0.0
76-77	0.0	0.0	0.0	2.067593449057351	0.0
78-79	0.0	0.0	0.0	2.206790852806341	0.0
80-81	0.0	0.0	0.0	2.3539777599233007	0.0
82-83	0.0	0.0	0.0	2.524232951412567	0.0
84-85	0.0	0.0	0.0	2.6772713257849405	0.0
86-87	0.0	0.0	0.0	2.835148413464676	0.0
88-89	0.0	0.0	0.0	3.017894236979923	0.0
90-91	0.0	0.0	0.0	3.207841866347987	0.0
92-93	0.0	0.0	0.0	3.397902023932501	0.0
94-95	0.0	0.0	0.0	3.584136222157706	0.0
96-97	0.0	0.0	0.0	3.7834236934911427	0.0
98-99	0.0	0.0	0.0	3.9946391557683087	0.0
100-101	0.0	0.0	0.0	4.230723353880986	0.0
102-103	0.0	0.0	0.0	4.474234414279381	0.0
104-105	0.0	0.0	0.0	4.717632946461325	0.0
106-107	0.0	0.0	0.0	4.966320304816433	0.0
108-109	0.0	0.0	0.0	5.226147956600117	0.0
110-111	0.0	0.0	0.0	5.511519513518015	0.0
112-113	0.0	0.0	0.0	5.806793553483536	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTAACG	110	7.7333226E-4	27.051655	2
CTAACGC	115	0.0010024953	25.875498	3
GGTATCA	2145	0.0	25.276712	1
GTATCAA	2705	0.0	24.2288	1
CGCCTAC	125	0.0016362044	23.789362	7
AACGCAG	2930	0.0	21.310595	6
CATGGGG	1780	0.0	20.729471	4
CAACGCA	3050	0.0	20.68343	5
ATCAACG	3115	0.0	20.633944	3
GTACTAG	145	0.0038334203	20.54511	1
TATCAAC	3220	0.0	20.145924	2
ACGCCTA	180	5.7977746E-4	19.822235	6
ACGCAGA	3155	0.0	19.79305	7
TCAACGC	3200	0.0	19.713894	4
CGCAGAG	3150	0.0	19.635664	8
GTACATG	4095	0.0	19.205538	1
AGTAGAG	155	0.005675254	19.197948	3
ACATGGG	4000	0.0	18.895582	3
TACATGG	4135	0.0	18.854383	2
ATTATAC	190	8.297892E-4	18.793783	3
>>END_MODULE