FastQCFastQC Report
Sun 1 Jan 2017
ERR1142913_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1142913_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences444333
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16280.36639187276209506No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14990.33735959291792417No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12390.2788449203637812No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA4610.10375101556715347No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4580.10307584626845182No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20400.035.31091
GTATCAA27950.033.44041
GTTAGTA953.281276E-431.3187523
TAGTCGT800.00535622929.7528154
TAGTATG1254.8765574E-528.5594865
TATCAAC35750.026.631892
ATCAACG35250.026.5032163
CAACGCA35750.026.2960325
TCAACGC36700.025.6182274
AACGCAG37300.025.3628146
TACCGTG1250.001632126223.7995727
TAGAACA1802.1715718E-523.1410774
ACGCAGA41350.022.5908897
CGCAGAG42500.022.1196028
ATTTAGG2454.3105683E-721.8690571
TTTAGGG2755.4906195E-821.6384092
ATAGGAC1653.2089336E-421.6384093
TAGTACT1653.2089336E-421.6384094
GCAGAGT44700.021.1640519
GAGTACT18350.020.91379412-13
Produced by FastQC (version 0.11.5)