##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1142913_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	444333
Sequences flagged as poor quality	0
Sequence length	125
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.254487062630954	33.0	14.0	33.0	14.0	33.0
2	27.69603653116019	33.0	27.0	33.0	14.0	33.0
3	29.853260955184513	33.0	27.0	33.0	27.0	33.0
4	30.727170838087652	33.0	27.0	33.0	27.0	33.0
5	31.640629887944403	33.0	33.0	33.0	27.0	33.0
6	35.04679823465734	37.0	37.0	37.0	33.0	37.0
7	35.90011545395008	37.0	37.0	37.0	33.0	37.0
8	36.04935712629942	37.0	37.0	37.0	33.0	37.0
9	36.12529341732439	37.0	37.0	37.0	37.0	37.0
10-11	36.15798849061402	37.0	37.0	37.0	37.0	37.0
12-13	36.19574733364391	37.0	37.0	37.0	37.0	37.0
14-15	36.22002979747171	37.0	37.0	37.0	37.0	37.0
16-17	36.22584300513354	37.0	37.0	37.0	37.0	37.0
18-19	36.21260518575033	37.0	37.0	37.0	37.0	37.0
20-21	36.141809183652796	37.0	37.0	37.0	37.0	37.0
22-23	36.18636698152062	37.0	37.0	37.0	37.0	37.0
24-25	36.21996565638834	37.0	37.0	37.0	37.0	37.0
26-27	36.14619553353003	37.0	37.0	37.0	37.0	37.0
28-29	36.146376703958516	37.0	37.0	37.0	37.0	37.0
30-31	36.105377048294855	37.0	37.0	37.0	37.0	37.0
32-33	36.063505298953714	37.0	37.0	37.0	37.0	37.0
34-35	35.995807198655065	37.0	37.0	37.0	37.0	37.0
36-37	35.93328764687745	37.0	37.0	37.0	37.0	37.0
38-39	35.87061955785413	37.0	37.0	37.0	37.0	37.0
40-41	35.80647847447747	37.0	37.0	37.0	33.0	37.0
42-43	35.730886519794836	37.0	37.0	37.0	33.0	37.0
44-45	35.62407023561158	37.0	37.0	37.0	33.0	37.0
46-47	35.49696398872017	37.0	37.0	37.0	33.0	37.0
48-49	35.282433445186385	37.0	37.0	37.0	33.0	37.0
50-51	35.23014945997709	37.0	37.0	37.0	33.0	37.0
52-53	35.206032412627465	37.0	37.0	37.0	33.0	37.0
54-55	35.13277091730751	37.0	37.0	37.0	33.0	37.0
56-57	35.103908555070184	37.0	37.0	37.0	33.0	37.0
58-59	35.06717034296349	37.0	37.0	37.0	33.0	37.0
60-61	35.03991938478573	37.0	37.0	37.0	33.0	37.0
62-63	35.017898738108585	37.0	37.0	37.0	33.0	37.0
64-65	34.99823780813039	37.0	37.0	37.0	30.0	37.0
66-67	34.97171603279523	37.0	37.0	37.0	27.0	37.0
68-69	34.89895528803848	37.0	37.0	37.0	27.0	37.0
70-71	34.88255767633734	37.0	37.0	37.0	27.0	37.0
72-73	34.87967695399621	37.0	37.0	37.0	27.0	37.0
74-75	34.83096236381273	37.0	37.0	37.0	27.0	37.0
76-77	34.775767273643865	37.0	37.0	37.0	27.0	37.0
78-79	34.69124958083239	37.0	37.0	37.0	27.0	37.0
80-81	34.626138504229935	37.0	37.0	37.0	27.0	37.0
82-83	34.6372315358076	37.0	37.0	37.0	27.0	37.0
84-85	34.58991342079027	37.0	37.0	37.0	27.0	37.0
86-87	34.51330533631308	37.0	37.0	37.0	27.0	37.0
88-89	34.462462837556515	37.0	37.0	37.0	27.0	37.0
90-91	34.4126904821384	37.0	37.0	37.0	27.0	37.0
92-93	34.31320878710336	37.0	37.0	37.0	27.0	37.0
94-95	34.26179239444291	37.0	37.0	37.0	27.0	37.0
96-97	34.20353878735092	37.0	37.0	37.0	27.0	37.0
98-99	34.1205379748972	37.0	37.0	37.0	24.5	37.0
100-101	34.054880011162794	37.0	37.0	37.0	22.0	37.0
102-103	33.97340733188847	37.0	37.0	37.0	22.0	37.0
104-105	33.84205652067256	37.0	35.0	37.0	22.0	37.0
106-107	33.7676033515404	37.0	33.0	37.0	22.0	37.0
108-109	33.69917269255266	37.0	33.0	37.0	22.0	37.0
110-111	33.65927693869238	37.0	33.0	37.0	22.0	37.0
112-113	33.59462160136654	37.0	33.0	37.0	22.0	37.0
114-115	33.443176626539106	37.0	33.0	37.0	22.0	37.0
116-117	33.40409670224808	37.0	33.0	37.0	22.0	37.0
118-119	33.23264871166445	37.0	33.0	37.0	14.0	37.0
120-121	33.14827055384137	37.0	33.0	37.0	14.0	37.0
122-123	33.07477725039554	37.0	33.0	37.0	14.0	37.0
124-125	31.437807905332264	35.0	27.5	37.0	14.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	6.0
15	147.0
16	65.0
17	12.0
18	43.0
19	281.0
20	1047.0
21	2463.0
22	4054.0
23	5266.0
24	4465.0
25	3348.0
26	3022.0
27	3452.0
28	4338.0
29	5730.0
30	7808.0
31	10868.0
32	15318.0
33	23382.0
34	39592.0
35	86021.0
36	223605.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	41.52716956918193	21.65685402618455	12.338710585268656	24.47726581936486
2	16.630957310815432	24.282061900217858	34.39261086404638	24.694369924920327
3	21.267028409001398	26.30809014000824	28.48462069943353	23.940260751556835
4	15.716931830202311	18.250483326093757	33.76575733091464	32.26682751278929
5	15.87040087863811	36.631377103194964	31.224286781717847	16.273935236449077
6	29.9637884199463	34.97354461631254	18.349751200113428	16.71291576362773
7	25.9107471198403	32.037908505557766	22.827473989102767	19.223870385499165
8	25.96341032513903	32.04285974708158	21.18658753682486	20.80714239095453
9	26.460334928983443	15.945473327436854	21.196940132738284	36.39725161084142
10-11	25.41157195166688	25.39570547314739	26.71757443178877	22.475148143396957
12-13	26.50532316524937	24.249574923227364	26.77235352433937	22.472748387183895
14-15	24.6151249345929	24.520263541379485	25.51670201932112	25.3479095047065
16-17	23.48711439393428	26.470012355598165	26.473163145658773	23.56971010480878
18-19	23.430989180399813	25.043520336684804	28.969184113248524	22.55630636966686
20-21	24.584766208746693	24.252579175744046	28.66981149061054	22.492843124898723
22-23	24.458457958333053	24.250280757900043	28.331566640335065	22.959694643431842
24-25	23.42989026223635	25.06155307292745	28.516852263622695	22.991704401213507
26-27	23.33964242953467	24.9961740320301	28.488945203136396	23.175238335298832
28-29	23.11948471079123	24.868060666212052	28.958011221313747	23.054443401682974
30-31	23.528458578083857	24.511399185290212	28.88247473724484	23.07766749938109
32-33	23.182838096652738	24.7036569419782	28.629653885711843	23.48385107565722
34-35	23.408344642419088	24.642329064012802	28.794507722811495	23.154818570756618
36-37	23.41256087790211	24.826818685127336	28.30034748791444	23.46027294905611
38-39	23.529650059752484	24.459020599415304	28.569001177045145	23.442328163787067
40-41	23.101705615127617	24.788078136235402	28.68813811465218	23.422078133984805
42-43	23.566671842964624	24.780851298463087	28.275077475677023	23.377399382895263
44-45	23.489252430046832	24.838240688852732	27.999945986456105	23.67256089464433
46-47	23.595366538159443	24.874137189900367	27.717950276031715	23.812545995908472
48-49	23.801630758912797	24.964272291277037	27.605984700663694	23.628112249146476
50-51	23.435040200750564	25.155260981359678	27.456128012243084	23.953570805646674
52-53	23.527022176110297	25.132474886317475	27.31643971422109	24.02406322335114
54-55	23.456619247276254	25.256283012965504	27.21641201531284	24.070685724445404
56-57	23.785314167527506	24.960221275484827	26.964461338680675	24.290003218306992
58-59	23.47293583866154	24.985090011320338	27.38903029934756	24.152943850670557
60-61	23.56993516124168	25.06340964996973	27.542631314802186	23.824023873986402
62-63	23.207958004422363	25.17540355477036	27.428671096532437	24.18796734427484
64-65	23.20016744198608	24.91678538393502	27.57999068266368	24.303056491415223
66-67	23.56025773462696	24.603956942203258	27.67473944091481	24.161045882254975
68-69	23.457857057657208	24.512133917579835	27.656059756984064	24.373949267778897
70-71	23.31652161779566	24.61633504601279	28.054634699650936	24.012508636540613
72-73	23.617984709665947	24.672261569588574	28.083104338412856	23.62664938233262
74-75	23.505906606081474	24.726275113484704	28.03111630241283	23.73670197802099
76-77	23.29035495980483	24.772467434260868	28.08620581006995	23.850971795864353
78-79	23.269034710453646	24.848593284766153	28.07196404498428	23.810407959795917
80-81	23.163033130557487	24.87852579034193	28.22038876248219	23.73805231661839
82-83	23.386626696644182	24.795254910168723	28.153321945477828	23.664796447709264
84-85	23.48790209144943	24.723011795207647	27.690268334784946	24.098817778557972
86-87	23.05039238589076	24.898105700004276	28.032354112793783	24.019147801311178
88-89	23.391915522817346	24.524399493172915	27.932991697668193	24.15069328634155
90-91	23.580512813588005	24.571886400514927	27.857485264430053	23.990115521467008
92-93	23.330362588419046	24.8471304179523	27.68768018580659	24.13482680782206
94-95	22.796191144929594	24.83362703197827	27.978790681763456	24.39139114132869
96-97	23.16010739692978	24.828225677588655	27.49356901242987	24.518097913051697
98-99	22.96114833311203	24.973499485743737	27.329063243392877	24.73628893775136
100-101	23.268126689337453	25.066673268351746	27.09905453366972	24.56614550864108
102-103	23.33547887615567	25.014403644121963	27.17202452220412	24.478092957518253
104-105	23.376068039443613	25.173266296146675	27.09154559500192	24.359120069407794
106-107	23.153234863198833	25.046193040773655	27.128874645253198	24.67169745077431
108-109	23.28582761783124	25.10327288685838	26.872105911676503	24.73879358363388
110-111	23.114758525700317	25.30478267425557	26.865999149286683	24.71445965075743
112-113	23.227218508766008	25.388787612810297	26.6096144757275	24.774379402696194
114-115	23.157000427610107	25.45439200594153	26.718767582652532	24.66983998379583
116-117	23.159978304364557	25.379167238690247	26.649397180917745	24.811457276027447
118-119	23.090426645241006	25.05193247315603	26.974390482685052	24.883250398917912
120-121	22.937958134625468	25.160748004294113	27.210805506730374	24.690488354350045
122-123	22.887722559692474	25.494183233217576	26.994569217161548	24.62352498992841
124-125	22.822094588207758	25.406008201185614	27.00730994810117	24.764587262505458
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	60.0
1	63.0
2	136.0
3	211.0
4	200.5
5	210.0
6	291.0
7	567.0
8	866.5
9	978.5
10	934.5
11	845.5
12	862.5
13	881.0
14	854.5
15	847.5
16	819.0
17	779.5
18	778.5
19	742.0
20	733.0
21	721.5
22	706.0
23	748.0
24	767.0
25	855.5
26	1024.5
27	1210.0
28	1472.5
29	2047.5
30	2887.0
31	3689.0
32	4799.5
33	5984.5
34	7243.0
35	8242.0
36	8874.5
37	9908.5
38	10338.0
39	10134.5
40	9808.5
41	9534.0
42	9782.5
43	10561.0
44	11833.5
45	13100.5
46	14718.0
47	16185.5
48	17387.0
49	18461.5
50	18965.5
51	18454.0
52	17892.0
53	17937.5
54	17604.0
55	17107.5
56	16262.5
57	14663.5
58	13432.0
59	12360.0
60	10624.0
61	8850.5
62	7204.0
63	5642.5
64	4239.0
65	3293.0
66	2534.5
67	1909.0
68	1543.5
69	1312.5
70	1176.5
71	1056.0
72	911.0
73	862.0
74	695.0
75	563.0
76	477.0
77	314.0
78	214.0
79	153.0
80	125.5
81	100.0
82	70.5
83	49.5
84	30.5
85	24.5
86	15.0
87	4.5
88	1.5
89	0.0
90	0.0
91	1.0
92	1.5
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.040060045056297865
2	0.0011252821645027491
3	4.5011286580109967E-4
4	0.0036009029264087973
5	0.0020255078961049482
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	3.375846493508247E-4
14-15	1.1252821645027492E-4
16-17	0.0
18-19	1.1252821645027492E-4
20-21	0.0011252821645027491
22-23	0.0
24-25	2.2505643290054983E-4
26-27	2.2505643290054983E-4
28-29	0.0
30-31	6.751692987016494E-4
32-33	0.0
34-35	0.0
36-37	2.2505643290054983E-4
38-39	0.0
40-41	0.001462866813853574
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	1.1252821645027492E-4
52-53	7.876975151519243E-4
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	1.1252821645027492E-4
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	2.2505643290054983E-4
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	4.5011286580109967E-4
100-101	4.5011286580109967E-4
102-103	2.2505643290054983E-4
104-105	5.626410822513746E-4
106-107	4.5011286580109967E-4
108-109	1.1252821645027492E-4
110-111	0.0
112-113	6.751692987016494E-4
114-115	6.751692987016494E-4
116-117	9.002257316021993E-4
118-119	0.001350338597403299
120-121	9.002257316021993E-4
122-123	0.0031507900606076973
124-125	0.0015753950303038487
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	444333.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	69.90909172465754
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.66986036181686	59.191930343482014
2	10.261683460585713	14.347699405909758
3	2.3915357864695204	5.0157028397729615
4	0.9282231615662041	2.5956495257153356
5	0.4383158541291775	1.5321131625344147
6	0.2673019293712909	1.1212101059157307
7	0.16252673578785548	0.7953467535931661
8	0.13046652163703593	0.729663682249645
9	0.08914481044400357	0.5608829457096352
>10	0.5858945233814313	7.9732914988375665
>50	0.05430526177529315	2.592119081103118
>100	0.01961023341547306	2.3933337078022747
>500	0.0	0.0
>1k	0.0011313596201234458	1.1510569473743921
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1628	0.36639187276209506	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1499	0.33735959291792417	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1239	0.2788449203637812	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	461	0.10375101556715347	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	458	0.10307584626845182	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	2.250564329005498E-4	0.0
5	0.0	0.0	0.0	2.250564329005498E-4	0.0
6	0.0	0.0	0.0	2.250564329005498E-4	0.0
7	0.0	0.0	0.0	2.250564329005498E-4	0.0
8	0.0	0.0	0.0	2.250564329005498E-4	0.0
9	0.0	0.0	0.0	2.250564329005498E-4	0.0
10-11	0.0	0.0	0.0	2.250564329005498E-4	0.0
12-13	0.0	0.0	0.0	2.250564329005498E-4	0.0
14-15	0.0	0.0	0.0	2.250564329005498E-4	0.0
16-17	0.0	0.0	0.0	7.876975151519243E-4	0.0
18-19	0.0	0.0	0.0	9.002257316021992E-4	0.0
20-21	0.0	0.0	0.0	0.001350338597403299	0.0
22-23	0.0	0.0	0.0	0.0033758464935082473	0.0
24-25	0.0	0.0	0.0	0.007089277636367319	0.0
26-27	0.0	0.0	0.0	0.013165801324682164	0.0
28-29	0.0	0.0	0.0	0.02205553042425388	0.0
30-31	0.0	0.0	0.0	0.039834988623397315	0.0
32-33	0.0	0.0	0.0	0.069992550632071	0.0
34-35	0.0	0.0	0.0	0.10498882594810649	0.0
36-37	0.0	0.0	0.0	0.15360101545462523	0.0
38-39	0.0	0.0	0.0	0.22134300175769073	0.0
40-41	0.0	0.0	0.0	0.2931360038529661	0.0
42-43	0.0	0.0	0.0	0.36166568767118357	0.0
44-45	0.0	0.0	0.0	0.42963273040714955	0.0
46-47	0.0	0.0	0.0	0.5102029333855465	0.0
48-49	0.0	0.0	0.0	0.5989876961648133	0.0
50-51	0.0	0.0	0.0	0.6897979668401852	0.0
52-53	0.0	0.0	0.0	0.7657545129441208	0.0
54-55	0.0	0.0	0.0	0.8464372441389678	0.0
56-57	0.0	0.0	0.0	0.9374725712472403	0.0
58-59	0.0	0.0	0.0	1.0360472888576813	0.0
60-61	0.0	0.0	0.0	1.1366475143642267	0.0
62-63	0.0	0.0	0.0	1.2389356631175268	2.250564329005498E-4
64-65	0.0	0.0	0.0	1.3312088006067522	2.250564329005498E-4
66-67	0.0	0.0	0.0	1.4376604933687123	2.250564329005498E-4
68-69	0.0	0.0	0.0	1.5588533824856583	2.250564329005498E-4
70-71	0.0	0.0	0.0	1.6869104928060712	2.250564329005498E-4
72-73	0.0	0.0	0.0	1.8166555263732382	4.501128658010996E-4
74-75	0.0	0.0	0.0	1.9417869030659438	4.501128658010996E-4
76-77	0.0	0.0	0.0	2.0735574445292153	4.501128658010996E-4
78-79	0.0	0.0	0.0	2.2146678279578604	4.501128658010996E-4
80-81	0.0	0.0	0.0	2.363430130105124	5.626410822513746E-4
82-83	0.0	0.0	0.0	2.535148188408243	6.751692987016494E-4
84-85	0.0	0.0	0.0	2.688861732079319	6.751692987016494E-4
86-87	0.0	0.0	0.0	2.847526517274207	6.751692987016494E-4
88-89	0.0	0.0	0.0	3.0318477358197566	6.751692987016494E-4
90-91	0.0	0.0	0.0	3.224270985949727	6.751692987016494E-4
92-93	0.0	0.0	0.0	3.414893784616493	6.751692987016494E-4
94-95	0.0	0.0	0.0	3.6023657932226505	6.751692987016494E-4
96-97	0.0	0.0	0.0	3.8041288853179935	6.751692987016494E-4
98-99	0.0	0.0	0.0	4.018045024789966	6.751692987016494E-4
100-101	0.0	0.0	0.0	4.2551419768506955	6.751692987016494E-4
102-103	0.0	0.0	0.0	4.499440734764242	6.751692987016494E-4
104-105	0.0	0.0	0.0	4.744977303058742	6.751692987016494E-4
106-107	0.0	0.0	0.0	4.994564887145452	7.876975151519243E-4
108-109	0.0	0.0	0.0	5.2547301235784865	9.002257316021992E-4
110-111	0.0	0.0	0.0	5.541114434444436	9.002257316021992E-4
112-113	0.0	0.0	0.0	5.840214433769267	9.002257316021992E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2040	0.0	35.3109	1
GTATCAA	2795	0.0	33.4404	1
GTTAGTA	95	3.281276E-4	31.318752	3
TAGTCGT	80	0.005356229	29.752815	4
TAGTATG	125	4.8765574E-5	28.559486	5
TATCAAC	3575	0.0	26.63189	2
ATCAACG	3525	0.0	26.503216	3
CAACGCA	3575	0.0	26.296032	5
TCAACGC	3670	0.0	25.618227	4
AACGCAG	3730	0.0	25.362814	6
TACCGTG	125	0.0016321262	23.799572	7
TAGAACA	180	2.1715718E-5	23.141077	4
ACGCAGA	4135	0.0	22.590889	7
CGCAGAG	4250	0.0	22.119602	8
ATTTAGG	245	4.3105683E-7	21.869057	1
TTTAGGG	275	5.4906195E-8	21.638409	2
ATAGGAC	165	3.2089336E-4	21.638409	3
TAGTACT	165	3.2089336E-4	21.638409	4
GCAGAGT	4470	0.0	21.164051	9
GAGTACT	1835	0.0	20.913794	12-13
>>END_MODULE