FastQCFastQC Report
Sun 1 Jan 2017
ERR1142907_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1142907_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences474103
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8740.18434812688382063No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12550.035.6024971
GTATCAA18400.035.2916221
ATCAACG21750.029.2895183
TCAACGC22100.028.8256594
CAACGCA22000.028.4154385
AACGCAG22400.027.8844686
TATCAAC23250.027.14382
ACGCAGA25050.024.9319847
CGCAGAG25850.024.1603958
GTTATAT1552.085885E-423.0612341
GCAGAGT27750.022.506179
CATGGGG16050.021.8859164
GTACATG33200.020.6358661
TACATGG33800.020.0805632
ATGGGGG8700.019.8457035
ACATGGG33350.019.8159483
CAGAGTA28150.019.23021310-11
TAATACC1550.00566289719.2055194
AGAGTAC27750.018.97148710-11
GACTGGG3804.26553E-918.7833467
Produced by FastQC (version 0.11.5)