##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1142907_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	474103
Sequences flagged as poor quality	0
Sequence length	125
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.25529895402476	33.0	33.0	33.0	27.0	33.0
2	31.41606359799453	33.0	33.0	33.0	27.0	33.0
3	31.51012543687764	33.0	33.0	33.0	27.0	33.0
4	31.51772926980002	33.0	33.0	33.0	27.0	33.0
5	31.41011763266632	33.0	33.0	33.0	27.0	33.0
6	34.75314435892623	37.0	37.0	37.0	27.0	37.0
7	34.74859893314322	37.0	37.0	37.0	27.0	37.0
8	34.83826088423824	37.0	37.0	37.0	27.0	37.0
9	34.89093509216352	37.0	37.0	37.0	27.0	37.0
10-11	34.87777550447899	37.0	37.0	37.0	27.0	37.0
12-13	34.861434118746345	37.0	37.0	37.0	27.0	37.0
14-15	34.86978462486	37.0	37.0	37.0	27.0	37.0
16-17	34.84601869214074	37.0	37.0	37.0	27.0	37.0
18-19	34.81548313341194	37.0	37.0	37.0	27.0	37.0
20-21	34.80487784300036	37.0	37.0	37.0	27.0	37.0
22-23	34.81484403178212	37.0	37.0	37.0	27.0	37.0
24-25	34.849088700134786	37.0	37.0	37.0	27.0	37.0
26-27	34.887647831800265	37.0	37.0	37.0	27.0	37.0
28-29	34.92528416820817	37.0	37.0	37.0	27.0	37.0
30-31	34.917900751524456	37.0	37.0	37.0	27.0	37.0
32-33	34.95399628350801	37.0	37.0	37.0	27.0	37.0
34-35	34.95944130283925	37.0	37.0	37.0	27.0	37.0
36-37	34.924102990278485	37.0	37.0	37.0	27.0	37.0
38-39	34.8925644849326	37.0	37.0	37.0	27.0	37.0
40-41	34.84381242050778	37.0	37.0	37.0	27.0	37.0
42-43	34.81872188111022	37.0	37.0	37.0	27.0	37.0
44-45	34.799518248144395	37.0	37.0	37.0	27.0	37.0
46-47	34.75985281679297	37.0	37.0	37.0	27.0	37.0
48-49	34.709851023933616	37.0	37.0	37.0	27.0	37.0
50-51	34.66497786346005	37.0	37.0	37.0	27.0	37.0
52-53	34.62807027164983	37.0	37.0	37.0	27.0	37.0
54-55	34.58825824768036	37.0	37.0	37.0	27.0	37.0
56-57	34.54635279675513	37.0	37.0	37.0	27.0	37.0
58-59	34.476484012967646	37.0	37.0	37.0	27.0	37.0
60-61	34.4369736112195	37.0	37.0	37.0	27.0	37.0
62-63	34.439004815409305	37.0	37.0	37.0	27.0	37.0
64-65	34.38941116170959	37.0	37.0	37.0	27.0	37.0
66-67	34.376000573715	37.0	37.0	37.0	27.0	37.0
68-69	34.298308595389614	37.0	37.0	37.0	27.0	37.0
70-71	34.22198551791489	37.0	37.0	37.0	27.0	37.0
72-73	34.1586522337973	37.0	37.0	37.0	27.0	37.0
74-75	34.112399626241555	37.0	37.0	37.0	27.0	37.0
76-77	34.02126225735758	37.0	37.0	37.0	27.0	37.0
78-79	33.97284872696439	37.0	37.0	37.0	27.0	37.0
80-81	33.88649091020306	37.0	35.0	37.0	27.0	37.0
82-83	33.80538300748994	37.0	33.0	37.0	27.0	37.0
84-85	33.72378575963451	37.0	33.0	37.0	22.0	37.0
86-87	33.63134593115842	37.0	33.0	37.0	22.0	37.0
88-89	33.546895927678165	37.0	33.0	37.0	22.0	37.0
90-91	33.43401644790267	37.0	33.0	37.0	22.0	37.0
92-93	33.37738529391292	37.0	33.0	37.0	22.0	37.0
94-95	33.29679415654404	37.0	33.0	37.0	22.0	37.0
96-97	33.195250821024125	37.0	33.0	37.0	14.0	37.0
98-99	33.05949234659979	37.0	33.0	37.0	14.0	37.0
100-101	32.937776179437805	37.0	33.0	37.0	14.0	37.0
102-103	32.845113825476744	37.0	33.0	37.0	14.0	37.0
104-105	32.653166084163146	37.0	33.0	37.0	14.0	37.0
106-107	32.60382237615033	37.0	33.0	37.0	14.0	37.0
108-109	32.452794013115295	37.0	33.0	37.0	14.0	37.0
110-111	32.290947325792075	37.0	33.0	37.0	14.0	37.0
112-113	32.152335041119755	37.0	33.0	37.0	14.0	37.0
114-115	32.0136953362455	37.0	33.0	37.0	14.0	37.0
116-117	31.915523630940957	37.0	30.0	37.0	14.0	37.0
118-119	31.79344045492224	37.0	27.0	37.0	14.0	37.0
120-121	31.691793766333475	37.0	27.0	37.0	14.0	37.0
122-123	31.472639911580394	37.0	27.0	37.0	14.0	37.0
124-125	29.46071528760628	35.0	20.5	37.0	14.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	128.0
1	15.0
2	3.0
3	15.0
4	9.0
5	7.0
6	12.0
7	8.0
8	8.0
9	14.0
10	12.0
11	7.0
12	4.0
13	6.0
14	37.0
15	215.0
16	423.0
17	704.0
18	1145.0
19	1784.0
20	2627.0
21	3401.0
22	4139.0
23	4893.0
24	5520.0
25	6045.0
26	7235.0
27	8514.0
28	10109.0
29	12310.0
30	14384.0
31	18164.0
32	23091.0
33	31971.0
34	47627.0
35	84866.0
36	184651.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.43895334724987	20.463635845556034	12.765921520040019	24.33148928715408
2	17.019386021380555	22.91286315811691	36.7040597726865	23.363691047816037
3	19.20015199012064	27.40867399174609	28.946518476299037	24.444655541834226
4	14.125570005066713	19.04534706975173	35.429403816922814	31.399679108258738
5	15.041773907829256	37.364263831206806	32.07079077996964	15.523171480994296
6	29.251367596407107	36.55640068886338	18.6995593300466	15.49267238468292
7	25.820855208737783	33.11823691274035	22.592087715448113	18.468820163073755
8	24.964920313723173	34.02803420823302	21.149305474963125	19.857740003080682
9	25.879252242575916	17.91697351956227	20.57852233713233	35.62525190072948
10-11	24.508044427141197	26.910136009850188	26.9133012379207	21.668518325087916
12-13	25.262905780181544	25.958958526989058	26.314950942894555	22.463184749934847
14-15	23.70956390904458	26.505732985425652	24.31224749699555	25.47245560853422
16-17	23.343814297019254	28.418992350303352	25.08520179372197	23.15199155895542
18-19	23.400806982594162	27.45629547718333	26.640449580470342	22.502447959752164
20-21	24.750326303605323	26.306559731233452	26.12254243529587	22.82057152986535
22-23	24.46756091461877	26.18909197571962	26.16556283704436	23.177784272617252
24-25	23.73252758081175	26.76602124173525	26.16328937776085	23.338161799692145
26-27	23.494593704894786	26.831957492424436	26.208714221805813	23.464734580874968
28-29	23.410341498414642	26.57261787313964	26.44990424538505	23.567136383060667
30-31	23.713287274047126	26.420501306232353	26.48908378035038	23.377127639370137
32-33	23.36890863145011	26.569102774451203	26.294453735406304	23.767534858692382
34-35	23.695311774587015	26.42244253674019	26.5449450057822	23.337300682890593
36-37	23.591675239376432	26.713302207802485	26.094800981245548	23.600221571575535
38-39	23.746117226011208	26.414258221351883	26.26897072726045	23.57065382537646
40-41	23.41057967466635	26.630361378658314	26.537505381427113	23.42155356524822
42-43	23.606589897783355	26.543089375989904	26.40169337538633	23.448627350840408
44-45	23.54929934154989	26.784146547357757	26.16706061117677	23.499493499915584
46-47	23.539696944116155	26.529630254938375	26.198189262198213	23.732483538747257
48-49	23.628470701700884	26.75852579396275	26.178901594731574	23.43410190960479
50-51	23.357970026529117	26.892230999601114	26.067232424195737	23.682566549674032
52-53	23.483455785356835	26.53638418627623	26.30833004426002	23.671829984106914
54-55	23.47128071789763	26.63033843504394	26.312079488759633	23.58630135829879
56-57	23.58014230496907	26.354593255339605	26.00627002911311	24.058994410578222
58-59	23.457622611404474	26.549975306765944	26.246691739796628	23.74571034203295
60-61	23.454502477692326	26.72129971213183	26.406417518593244	23.4177802915826
62-63	23.23145874379883	26.741367429187118	26.10291440613843	23.92425942087562
64-65	23.367379565717954	26.410857367778455	26.222282254839165	23.99948081166443
66-67	23.618737891533463	26.269093917381742	26.15133314480354	23.96083504628125
68-69	23.533117369603985	26.30396015574713	26.06020956219861	24.102712912450276
70-71	23.269134176659534	26.38560014182062	26.386022227005856	23.959243454513988
72-73	23.500623619016263	26.440074538827936	26.30142218901882	23.757879653136982
74-75	23.55000163564395	26.147298707630227	26.545551628837565	23.757148027888256
76-77	23.38696656532388	26.22579566044645	26.503230762899044	23.88400701133062
78-79	23.540572511552273	26.18532811199083	26.38888449204294	23.885214884413955
80-81	23.323135823861328	26.443172716403357	26.484645167826244	23.749046291909075
82-83	23.54087697230125	26.478012376216725	26.348847222104972	23.63226342937705
84-85	23.502910596767318	26.32580954270494	26.291089225937448	23.8801906345903
86-87	23.13569472512281	26.518011196648395	26.44688451411717	23.89940956411163
88-89	23.280777271129974	26.067750571693594	26.510120585929215	24.141351571247217
90-91	23.63872174182832	26.163093788320413	26.391776294267	23.806408175584266
92-93	23.40189322386264	26.458911554575295	26.32457075317384	23.814624468388228
94-95	22.867219430963033	26.52244984967302	26.590833061594488	24.019497657769463
96-97	23.125476882227108	26.39629533085469	26.39355144418143	24.084676342736774
98-99	22.889520267317934	26.479335842429336	26.399440202810386	24.231703687442348
100-101	23.17969899263205	26.690079757877115	26.267124516419067	23.863096733071764
102-103	23.400806469485126	26.610221541663194	26.3784750107378	23.610496978113883
104-105	23.22572883158988	26.628209349644976	26.35962023927121	23.786441579493932
106-107	23.160263161782865	26.464560331126247	26.514689400287477	23.86048710680341
108-109	23.231123986217302	26.398716710726966	26.41697403344362	23.953185269612113
110-111	23.078368033533383	26.732468475389748	26.296943941122404	23.89221954995446
112-113	23.1457081194033	26.708177448036768	26.20772628212908	23.93838815043085
114-115	23.075429283068253	26.798028516849428	26.37808571941193	23.748456480670388
116-117	23.129146883169103	26.863729474676244	26.16350183473361	23.84362180742104
118-119	22.953115468961396	26.451145149341148	26.423600777574446	24.172138604123003
120-121	22.8964511434665	26.36658255953719	26.702905547799126	24.034060749197184
122-123	23.054762645955456	26.590617695631757	26.50176492003778	23.852854738375008
124-125	23.04411609498681	26.30722955145119	26.40527704485488	24.243377308707124
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	505.0
1	375.5
2	225.5
3	172.0
4	108.5
5	85.0
6	114.0
7	183.5
8	262.0
9	271.0
10	196.0
11	130.5
12	127.0
13	137.0
14	145.5
15	160.5
16	185.5
17	209.5
18	212.5
19	246.0
20	325.0
21	392.0
22	475.0
23	570.0
24	729.5
25	906.0
26	1188.0
27	1688.5
28	2272.5
29	3108.0
30	4353.0
31	5640.0
32	7067.0
33	8639.5
34	10041.5
35	11119.5
36	11723.5
37	12364.0
38	12466.0
39	12049.5
40	11971.0
41	11977.0
42	12203.0
43	12920.0
44	14018.5
45	14867.0
46	15908.0
47	17118.5
48	17883.5
49	18541.5
50	18568.0
51	17793.5
52	17398.0
53	17517.0
54	17037.5
55	16444.0
56	15762.0
57	14494.0
58	13312.5
59	12017.5
60	10519.0
61	8781.0
62	7074.0
63	5512.0
64	4251.5
65	3488.0
66	2729.5
67	2084.0
68	1735.5
69	1465.0
70	1265.0
71	1177.0
72	1062.5
73	1011.5
74	794.0
75	643.0
76	544.0
77	402.0
78	307.0
79	183.5
80	123.0
81	99.5
82	74.0
83	41.5
84	23.5
85	16.0
86	11.5
87	6.5
88	2.0
89	1.0
90	1.5
91	1.5
92	1.0
93	0.0
94	0.5
95	0.5
96	0.0
97	0.0
98	0.5
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.06876142947840448
2	0.0649647861329711
3	0.08183875655711945
4	0.08922111861768434
5	0.10166567180549374
6	0.05884797185421733
7	0.04366139847248383
8	0.03838828271493747
9	0.04345047384218197
10-11	0.04292316226642733
12-13	0.04566518246035144
14-15	0.04693073024216257
16-17	0.048090815708822766
18-19	0.05041098664214316
20-21	0.049461825805784815
22-23	0.04693073024216257
24-25	0.037439121878579125
26-27	0.04450509699369124
28-29	0.049672750436086675
30-31	0.046614343296709784
32-33	0.04756350413306813
34-35	0.04956728812093575
36-37	0.04545425783004959
38-39	0.04534879551489866
40-41	0.05294208220576541
42-43	0.05410216767242561
44-45	0.05547317776938767
46-47	0.05547317776938767
48-49	0.05515679082393488
50-51	0.0599025950057266
52-53	0.06580848465417852
54-55	0.05800427333300991
56-57	0.05515679082393488
58-59	0.06095721815723588
60-61	0.05800427333300991
62-63	0.06359377603600905
64-65	0.06042990658148124
66-67	0.05505132850878396
68-69	0.054418554617878395
70-71	0.05589502702999138
72-73	0.05399670535727469
74-75	0.059797132690575676
76-77	0.06296100214510349
78-79	0.0587425095390664
80-81	0.06211730362389608
82-83	0.061379067417839585
84-85	0.0668631078056878
86-87	0.0633828514057072
88-89	0.06148452973299051
90-91	0.06369923835115998
92-93	0.0649647861329711
94-95	0.0638047006663109
96-97	0.06802319327234799
98-99	0.07593286690866753
100-101	0.06201184130874514
102-103	0.06528117307842389
104-105	0.06981605262991375
106-107	0.06876142947840448
108-109	0.06760134401174428
110-111	0.06464839918751832
112-113	0.06971059031476283
114-115	0.07340177134504527
116-117	0.06928874105415912
118-119	0.06791773095719707
120-121	0.0638047006663109
122-123	0.06021898195117939
124-125	0.07445639449655454
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	474103.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	78.27416049920956
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.69350005687377	69.4240925868839
2	7.848220093732178	12.28625678499828
3	1.6444482894063435	3.8615342801292813
4	0.6111516509121558	1.9134952965141991
5	0.33766721442603975	1.321530886865242
6	0.18089157106334378	0.8495481519819804
7	0.12925865358658467	0.708232881772364
8	0.0807903815877649	0.5059039436154475
9	0.06817885461218719	0.48029783477099514
>10	0.3612678252499895	5.274410749675928
>50	0.03362618818902603	1.8060370667456545
>100	0.010684956921728219	1.3536668381472872
>500	3.142634388743594E-4	0.2149926978994601
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	874	0.18434812688382063	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	1.0546231515092713E-4
16-17	0.0	0.0	0.0	0.0	2.1092463030185425E-4
18-19	0.0	0.0	0.0	0.0	2.1092463030185425E-4
20-21	0.0	0.0	0.0	2.1092463030185425E-4	2.1092463030185425E-4
22-23	0.0	0.0	0.0	8.43698521207417E-4	2.1092463030185425E-4
24-25	0.0	0.0	0.0	0.0020037839878676153	2.1092463030185425E-4
26-27	0.0	0.0	0.0	0.0023201709333203965	2.1092463030185425E-4
28-29	0.0	0.0	0.0	0.004640341866640793	2.1092463030185425E-4
30-31	0.0	0.0	0.0	0.01022984456963993	2.1092463030185425E-4
32-33	0.0	0.0	0.0	0.020987000715034496	2.1092463030185425E-4
34-35	0.0	0.0	0.0	0.031744156860429064	4.218492606037085E-4
36-37	0.0	0.0	0.0	0.049989137381539456	4.218492606037085E-4
38-39	0.0	0.0	0.0	0.07740933932078051	4.218492606037085E-4
40-41	0.0	0.0	0.0	0.10915349618120956	4.218492606037085E-4
42-43	0.0	0.0	0.0	0.1353081503386395	4.218492606037085E-4
44-45	0.0	0.0	0.0	0.1652594478415028	4.218492606037085E-4
46-47	0.0	0.0	0.0	0.1991128510049504	4.218492606037085E-4
48-49	0.0	0.0	0.0	0.23792298298049158	4.218492606037085E-4
50-51	1.0546231515092713E-4	0.0	0.0	0.2809516075620698	4.218492606037085E-4
52-53	2.1092463030185425E-4	0.0	0.0	0.32398023214364813	4.218492606037085E-4
54-55	2.1092463030185425E-4	0.0	0.0	0.36036473087071796	5.273115757546356E-4
56-57	2.1092463030185425E-4	0.0	0.0	0.40750638574318243	6.327738909055628E-4
58-59	2.1092463030185425E-4	0.0	0.0	0.45791737238532554	6.327738909055628E-4
60-61	2.1092463030185425E-4	0.0	0.0	0.5157107210880336	6.327738909055628E-4
62-63	2.1092463030185425E-4	0.0	0.0	0.5680200294028934	6.327738909055628E-4
64-65	2.1092463030185425E-4	0.0	0.0	0.6207511869783571	6.327738909055628E-4
66-67	2.1092463030185425E-4	0.0	0.0	0.6840285760689133	7.382362060564899E-4
68-69	2.1092463030185425E-4	0.0	0.0	0.7531063924927706	8.43698521207417E-4
70-71	2.1092463030185425E-4	0.0	0.0	0.8254535406863066	8.43698521207417E-4
72-73	2.1092463030185425E-4	0.0	0.0	0.8979061511949935	0.0010546231515092713
74-75	2.1092463030185425E-4	0.0	0.0	0.9710969979097369	0.0010546231515092713
76-77	2.1092463030185425E-4	0.0	0.0	1.0563105485516862	0.0010546231515092713
78-79	2.1092463030185425E-4	0.0	0.0	1.1493283105148038	0.0011600854666601983
80-81	2.1092463030185425E-4	0.0	0.0	1.2503612084293918	0.0012655477818111255
82-83	2.1092463030185425E-4	0.0	0.0	1.3600420161863562	0.0012655477818111255
84-85	2.1092463030185425E-4	0.0	0.0	1.463078698088812	0.0012655477818111255
86-87	2.1092463030185425E-4	0.0	0.0	1.5680137016639843	0.0012655477818111255
88-89	2.1092463030185425E-4	0.0	0.0	1.6894008264027014	0.0012655477818111255
90-91	2.1092463030185425E-4	0.0	0.0	1.8240762028504354	0.0012655477818111255
92-93	2.1092463030185425E-4	0.0	0.0	1.9598062024496787	0.001687397042414834
94-95	2.1092463030185425E-4	0.0	0.0	2.090368548606526	0.001687397042414834
96-97	2.1092463030185425E-4	0.0	0.0	2.2324262871148255	0.001687397042414834
98-99	2.1092463030185425E-4	0.0	0.0	2.3880886642775936	0.001687397042414834
100-101	2.1092463030185425E-4	0.0	0.0	2.551238865816078	0.001687397042414834
102-103	2.1092463030185425E-4	0.0	0.0	2.7255680727605602	0.001687397042414834
104-105	2.1092463030185425E-4	0.0	0.0	2.9137128429898143	0.001687397042414834
106-107	2.1092463030185425E-4	0.0	0.0	3.096479035146371	0.001687397042414834
108-109	2.1092463030185425E-4	0.0	0.0	3.277874217205966	0.001687397042414834
110-111	2.1092463030185425E-4	0.0	0.0	3.4852131287926884	0.001687397042414834
112-113	2.1092463030185425E-4	0.0	0.0	3.6999344024399763	0.001687397042414834
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1255	0.0	35.602497	1
GTATCAA	1840	0.0	35.291622	1
ATCAACG	2175	0.0	29.289518	3
TCAACGC	2210	0.0	28.825659	4
CAACGCA	2200	0.0	28.415438	5
AACGCAG	2240	0.0	27.884468	6
TATCAAC	2325	0.0	27.1438	2
ACGCAGA	2505	0.0	24.931984	7
CGCAGAG	2585	0.0	24.160395	8
GTTATAT	155	2.085885E-4	23.061234	1
GCAGAGT	2775	0.0	22.50617	9
CATGGGG	1605	0.0	21.885916	4
GTACATG	3320	0.0	20.635866	1
TACATGG	3380	0.0	20.080563	2
ATGGGGG	870	0.0	19.845703	5
ACATGGG	3335	0.0	19.815948	3
CAGAGTA	2815	0.0	19.230213	10-11
TAATACC	155	0.005662897	19.205519	4
AGAGTAC	2775	0.0	18.971487	10-11
GACTGGG	380	4.26553E-9	18.783346	7
>>END_MODULE