FastQCFastQC Report
Sun 1 Jan 2017
ERR1142907_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1142907_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences474103
Sequences flagged as poor quality0
Sequence length125
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9280.19573805692012072No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6420.13541361265379043No Hit
AATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5480.11558669740541613No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCCGA902.3908829E-433.0555531
ATCAACG24100.031.354772
TCAACGC24200.030.733473
CAACGCA24700.030.3522284
AATCAAC6850.028.6642341
GTAATAC1055.9004885E-428.3333363
AGTATCA3150.028.3333321
AACGCAG27300.027.243595
TATCAAC22250.025.4044951
GTATCAA14950.023.8795971
ACGCAGA32800.022.8567076
CGCAGAG33100.022.6495467
ATACATG4250.022.41
GGTATCA11200.022.31251
AGAGTAC33600.021.9583345
GCAGAGT35100.021.528498
CAGAGTA35400.021.1779674
ATATATC3451.2769306E-920.6956523
AGTACAT27250.019.4330277
GAGTACA27400.019.3266436
Produced by FastQC (version 0.11.5)