##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1142907_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	474103
Sequences flagged as poor quality	0
Sequence length	125
%GC	46
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	21.699322721012102	14.0	14.0	33.0	14.0	33.0
2	22.854961896465536	27.0	14.0	27.0	14.0	33.0
3	25.97622668491868	27.0	27.0	33.0	14.0	33.0
4	26.682229388972438	27.0	27.0	33.0	14.0	33.0
5	29.586193295549702	33.0	27.0	33.0	14.0	33.0
6	32.68836096797531	37.0	33.0	37.0	27.0	37.0
7	34.733300569707424	37.0	37.0	37.0	27.0	37.0
8	34.94029567414676	37.0	37.0	37.0	27.0	37.0
9	34.87404424776894	37.0	37.0	37.0	27.0	37.0
10-11	35.1526820121366	37.0	37.0	37.0	33.0	37.0
12-13	35.281592818438185	37.0	37.0	37.0	33.0	37.0
14-15	35.32363747961941	37.0	37.0	37.0	33.0	37.0
16-17	35.437737158381196	37.0	37.0	37.0	33.0	37.0
18-19	35.4192591061436	37.0	37.0	37.0	33.0	37.0
20-21	35.41350402760581	37.0	37.0	37.0	33.0	37.0
22-23	35.40317293921363	37.0	37.0	37.0	33.0	37.0
24-25	35.3965467419527	37.0	37.0	37.0	33.0	37.0
26-27	35.2887632012453	37.0	37.0	37.0	33.0	37.0
28-29	35.27462386865301	37.0	37.0	37.0	33.0	37.0
30-31	35.271134120644675	37.0	37.0	37.0	33.0	37.0
32-33	35.282662206313816	37.0	37.0	37.0	33.0	37.0
34-35	35.23729548220534	37.0	37.0	37.0	33.0	37.0
36-37	35.16668002522658	37.0	37.0	37.0	33.0	37.0
38-39	35.122773954182954	37.0	37.0	37.0	33.0	37.0
40-41	35.04072216374922	37.0	37.0	37.0	30.0	37.0
42-43	34.919966758278264	37.0	37.0	37.0	27.0	37.0
44-45	34.81155466217257	37.0	37.0	37.0	27.0	37.0
46-47	34.6944988747171	37.0	37.0	37.0	27.0	37.0
48-49	34.48274952911076	37.0	37.0	37.0	27.0	37.0
50-51	34.34842112367988	37.0	37.0	37.0	27.0	37.0
52-53	34.23666587218389	37.0	37.0	37.0	27.0	37.0
54-55	34.067546503607865	37.0	35.0	37.0	27.0	37.0
56-57	33.96425143903329	37.0	33.0	37.0	27.0	37.0
58-59	33.86813308500473	37.0	33.0	37.0	27.0	37.0
60-61	33.74783854985098	37.0	33.0	37.0	27.0	37.0
62-63	33.684763648405514	37.0	33.0	37.0	27.0	37.0
64-65	33.60738805702559	37.0	33.0	37.0	27.0	37.0
66-67	33.5346612444975	37.0	33.0	37.0	27.0	37.0
68-69	33.409370959475055	37.0	33.0	37.0	22.0	37.0
70-71	33.31861220030247	37.0	33.0	37.0	22.0	37.0
72-73	33.249432085432915	37.0	33.0	37.0	22.0	37.0
74-75	33.14261036103969	37.0	33.0	37.0	14.0	37.0
76-77	33.02295070902315	37.0	33.0	37.0	14.0	37.0
78-79	32.887957891006806	37.0	33.0	37.0	14.0	37.0
80-81	32.7522553116095	37.0	33.0	37.0	14.0	37.0
82-83	32.72091191154665	37.0	33.0	37.0	14.0	37.0
84-85	32.62918078982837	37.0	33.0	37.0	14.0	37.0
86-87	32.49361214757131	37.0	33.0	37.0	14.0	37.0
88-89	32.371787354224715	37.0	33.0	37.0	14.0	37.0
90-91	32.2769514219484	37.0	33.0	37.0	14.0	37.0
92-93	32.09329512785196	37.0	30.0	37.0	14.0	37.0
94-95	32.01430490842707	37.0	30.0	37.0	14.0	37.0
96-97	31.882427447200293	37.0	27.0	37.0	14.0	37.0
98-99	31.749713669814366	37.0	27.0	37.0	14.0	37.0
100-101	31.655728818421316	37.0	27.0	37.0	14.0	37.0
102-103	31.520679050754794	37.0	27.0	37.0	14.0	37.0
104-105	31.222854527391725	37.0	27.0	37.0	14.0	37.0
106-107	31.107615855626307	37.0	27.0	37.0	14.0	37.0
108-109	30.996077856499536	37.0	27.0	37.0	14.0	37.0
110-111	30.930790355682205	37.0	27.0	37.0	14.0	37.0
112-113	30.812819155331226	37.0	27.0	37.0	14.0	37.0
114-115	30.580456145605492	37.0	27.0	37.0	14.0	37.0
116-117	30.495561091155295	37.0	27.0	37.0	14.0	37.0
118-119	30.267197212420086	35.0	22.0	37.0	14.0	37.0
120-121	30.079977346694704	33.0	22.0	37.0	14.0	37.0
122-123	29.974793452055778	33.0	22.0	37.0	14.0	37.0
124-125	28.085824177446675	30.0	18.0	37.0	14.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	6.0
15	199.0
16	73.0
17	44.0
18	124.0
19	499.0
20	1474.0
21	2686.0
22	3875.0
23	4328.0
24	4757.0
25	5701.0
26	7273.0
27	9990.0
28	13782.0
29	18526.0
30	25570.0
31	34462.0
32	46196.0
33	61610.0
34	81775.0
35	100587.0
36	50566.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.712620179375037	40.49166735235105	9.078461282039923	22.717251186233987
2	11.405634688113665	34.83259825479488	27.493835675671114	26.267931381420333
3	22.657734711655483	27.82559907868121	26.3904678941074	23.1261983155559
4	17.297442095427325	19.240707109696498	32.235461369882664	31.22638942499351
5	16.485621116309286	39.308961438352405	28.90351783808411	15.301899607254196
6	30.355850943782258	36.84916568762484	17.29476506160054	15.500218306992364
7	25.636834190038872	32.75153289475072	22.456090765086913	19.155542150123498
8	25.681339287032568	33.2952965916689	21.068206697700713	19.955157423597825
9	25.41915997156736	16.731385374064285	21.047957933191732	36.801496721176626
10-11	25.018297711678684	26.54518111043381	26.948574465886104	21.487946712001403
12-13	25.42846928024754	24.86477023331551	27.226927623995334	22.47983286244161
14-15	23.773209619006842	25.54687483521513	25.103827649266087	25.57608789651194
16-17	23.446381904354116	27.40427712965326	25.930019426158452	23.21932153983417
18-19	23.462883514517973	26.370169288465352	27.99735078105555	22.169596415961124
20-21	24.07384947674486	25.762208144071025	27.557717314614337	22.60622506456978
22-23	23.614383372389543	25.877921042473893	27.578922723543197	22.928772861593366
24-25	23.292245128685387	26.30035308857588	27.489865050136892	22.917536732601846
26-27	23.230914931628494	26.3919502384513	27.420423074408195	22.95671175551201
28-29	22.94849431452659	26.148853730096626	27.951942932232026	22.95070902314476
30-31	23.36908891295796	25.666712718981394	27.91782720257499	23.046371165485656
32-33	23.05416755430782	26.01966239403674	27.656753912124582	23.26941613953086
34-35	23.219743389094774	25.820655005346936	27.93063954457154	23.028962060986746
36-37	23.16421360804215	26.107356644772644	27.510746632581174	23.21768311460403
38-39	23.430035245505724	25.848180669601334	27.62300597127628	23.09877811361666
40-41	22.959306893624202	26.130806426948045	27.994874472023163	22.915012207404594
42-43	23.58838393425887	25.94188592328233	27.60935410523474	22.860376037224057
44-45	23.364648610112148	26.104559557733236	27.057517037437012	23.473274794717604
46-47	23.31771787986999	26.213923978544745	27.137457472321415	23.33090066926385
48-49	23.508921057238616	26.149170117042075	27.061208218467296	23.28070060725201
50-51	23.295936317501297	26.20881160594134	26.999886100459396	23.495365976097972
52-53	23.18364316501846	26.112340922777083	27.13912527169815	23.564890640506306
54-55	23.47622773954183	26.119957055745267	27.118790642539704	23.2850245621732
56-57	23.486879433372074	25.986230840133896	26.911768117898433	23.6151216085956
58-59	23.213626574816022	26.02293172580642	27.272449235714603	23.49099246366296
60-61	23.29346154738527	26.178805027599488	27.369474565653455	23.158258859361787
62-63	23.110873937331984	26.18416098662417	27.125626052649064	23.57933902339478
64-65	23.05733142376235	25.78311042115321	27.43475573873188	23.724802416352563
66-67	23.345559931069833	25.698213257456715	27.377806088550376	23.578420722923077
68-69	23.414426822863383	25.719516645117203	27.16171380480613	23.704342727213284
70-71	22.92919471085397	25.714454453989955	27.74829520167559	23.608055633480486
72-73	23.244632495470395	25.752526349759442	27.637454308451957	23.365386846318206
74-75	23.22586020337353	25.706333855723333	27.526613415228336	23.541192525674802
76-77	23.108058797349944	25.663410693456907	27.803030143238917	23.425500365954232
78-79	23.1259873909256	25.685979628899208	27.77497716740877	23.413055812766423
80-81	23.012826326768657	25.806523055116713	27.768649428499714	23.412001189614916
82-83	23.089075580622776	25.901544601067698	27.832770516111477	23.176609302198045
84-85	23.188210156864645	25.70116620228094	27.5228167718829	23.587806868971512
86-87	22.890911890454184	25.855668493977046	27.668038379845726	23.585381235723037
88-89	22.91907032859948	25.64569302451155	27.591472739046157	23.84376390784281
90-91	23.22491104253717	25.730168338947447	27.643676585045867	23.40124403346952
92-93	23.202763956355476	25.79892976842585	27.576497090294726	23.42180918492395
94-95	22.55585811521969	26.041914942533584	27.71148885368791	23.690738088558817
96-97	22.935522449763027	25.923480762619093	27.452262483046937	23.688734304570946
98-99	22.74869120990842	25.859519681418767	27.490075975212086	23.90171313346073
100-101	23.072566662870013	26.0282597415746	27.152912242513217	23.746261353042172
102-103	23.12242605765631	25.95957625197083	27.397661898007286	23.520335792365575
104-105	22.845822207035617	26.097548628776085	27.262593281614507	23.79403588257379
106-107	22.920149123870893	25.96400567387854	27.258873344899047	23.85697185735152
108-109	22.930216419901644	26.116032115485822	27.070047236720406	23.883704227892128
110-111	23.002937128574516	26.293364831444677	26.939216730559316	23.764481309421488
112-113	22.82691117178742	26.335766361773505	26.895352356726054	23.941970109713026
114-115	22.856838521749584	26.151178757269022	27.159719131577447	23.832263589403947
116-117	22.858550033168072	26.094467312310364	27.045215096799097	24.001767557722467
118-119	22.761457295176626	25.91302421970164	27.221826733952405	24.103691751169325
120-121	22.757694068116574	25.87413177416531	27.374978643701	23.993195514017117
122-123	22.67648191017576	26.120641014148084	27.115067207348776	24.087809868327383
124-125	22.80364271062387	25.74818470884154	27.158232220165683	24.28994036036891
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	380.0
1	295.0
2	247.5
3	308.0
4	310.0
5	284.5
6	444.5
7	971.0
8	1173.0
9	962.0
10	836.5
11	771.5
12	762.5
13	714.5
14	655.5
15	599.0
16	562.5
17	533.5
18	506.0
19	494.0
20	480.0
21	460.5
22	457.0
23	537.5
24	670.5
25	788.0
26	1030.5
27	1427.5
28	1964.0
29	2811.0
30	4075.5
31	5322.0
32	6784.0
33	8538.0
34	9948.0
35	10823.0
36	11501.5
37	12144.5
38	12194.5
39	11803.5
40	11614.0
41	11658.0
42	12066.0
43	13001.0
44	13924.5
45	14769.5
46	15907.0
47	17011.0
48	18048.0
49	18703.5
50	18521.5
51	17849.0
52	17387.5
53	17102.5
54	16545.5
55	16022.0
56	15301.5
57	14280.5
58	13239.5
59	11975.0
60	10355.5
61	8551.0
62	6852.0
63	5287.5
64	4107.5
65	3337.0
66	2633.5
67	2018.5
68	1636.0
69	1389.5
70	1196.5
71	1133.0
72	1125.0
73	1044.5
74	795.0
75	636.0
76	523.5
77	375.0
78	255.5
79	165.5
80	114.0
81	80.5
82	61.0
83	44.0
84	29.5
85	19.0
86	12.5
87	5.0
88	2.5
89	3.0
90	3.0
91	1.5
92	0.0
93	0.0
94	0.0
95	0.5
96	0.5
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.026154654157429926
2	8.43698521207417E-4
3	0.0
4	8.43698521207417E-4
5	0.001054623151509271
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	5.273115757546355E-4
14-15	0.0
16-17	0.0
18-19	2.1092463030185425E-4
20-21	7.382362060564898E-4
22-23	0.0
24-25	2.1092463030185425E-4
26-27	4.218492606037085E-4
28-29	0.0
30-31	9.49160836358344E-4
32-33	0.0
34-35	0.0
36-37	2.1092463030185425E-4
38-39	0.0
40-41	0.0011600854666601983
42-43	2.1092463030185425E-4
44-45	0.0
46-47	0.0
48-49	0.0
50-51	2.1092463030185425E-4
52-53	3.163869454527814E-4
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	3.163869454527814E-4
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	2.1092463030185425E-4
100-101	2.1092463030185425E-4
102-103	1.0546231515092713E-4
104-105	2.1092463030185425E-4
106-107	1.0546231515092713E-4
108-109	3.163869454527814E-4
110-111	1.0546231515092713E-4
112-113	5.273115757546355E-4
114-115	4.218492606037085E-4
116-117	5.273115757546355E-4
118-119	0.0011600854666601983
120-121	8.43698521207417E-4
122-123	0.0022147086181694693
124-125	0.0011600854666601983
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	474103.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	80.60329669129005
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.112987765852	72.6340388862956
2	6.9359975113857875	11.181285305205561
3	1.431622882763713	3.4618057190833045
4	0.5178227043931324	1.6695286830274332
5	0.27255820290515975	1.0984544847204711
6	0.16178208717736456	0.782410174325596
7	0.10248219480112773	0.5782281927190915
8	0.08273315352689563	0.5334851935947534
9	0.05040410217858106	0.3656463122122441
>10	0.29427971668390646	4.392676973660917
>50	0.025866234003159842	1.3786425870888506
>100	0.010581640919217794	1.4219990792331734
>500	8.818034099348151E-4	0.5017984088330322
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	928	0.19573805692012072	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	642	0.13541361265379043	No Hit
AATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	548	0.11558669740541613	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	2.1092463030185425E-4	0.0
22-23	0.0	0.0	0.0	8.43698521207417E-4	0.0
24-25	0.0	0.0	0.0	0.0017928593575657612	0.0
26-27	0.0	0.0	0.0	0.0021092463030185425	0.0
28-29	0.0	0.0	0.0	0.004429417236338939	0.0
30-31	0.0	0.0	0.0	0.010124382254489004	0.0
32-33	0.0	0.0	0.0	0.020776076084732642	0.0
34-35	0.0	0.0	0.0	0.031427769914976283	0.0
36-37	0.0	0.0	0.0	0.04925090117548296	0.0
38-39	0.0	0.0	0.0	0.07561647996321474	0.0
40-41	0.0	0.0	0.0	0.10651693830243639	0.0
42-43	0.0	0.0	0.0	0.13161696930835703	0.0
44-45	0.0	0.0	0.0	0.16083003060516388	0.0
46-47	0.0	0.0	0.0	0.19405065987770592	0.0
48-49	0.0	0.0	0.0	0.2321225556471906	0.0
50-51	0.0	0.0	0.0	0.27409655707725955	0.0
52-53	0.0	0.0	0.0	0.31617602082247953	0.0
54-55	0.0	0.0	0.0	0.35108404713743635	0.0
56-57	0.0	0.0	0.0	0.396432842652335	0.0
58-59	0.0	0.0	0.0	0.4463165177187236	0.0
60-61	0.0	0.0	0.0	0.5032661679002242	0.0
62-63	0.0	0.0	0.0	0.5545208530635748	0.0
64-65	0.0	0.0	0.0	0.606408312117831	0.0
66-67	0.0	0.0	0.0	0.6672600679599159	0.0
68-69	0.0	0.0	0.0	0.7347559496565093	0.0
70-71	0.0	0.0	0.0	0.8049938515470267	0.0
72-73	0.0	0.0	0.0	0.8742825926011858	0.0
74-75	0.0	0.0	0.0	0.9446259568068542	0.0
76-77	0.0	0.0	0.0	1.0278357234609357	0.0
78-79	0.0	0.0	0.0	1.118322389860431	0.0
80-81	0.0	0.0	0.0	1.2162968806356425	3.163869454527814E-4
82-83	0.0	0.0	0.0	1.3215482711562678	6.327738909055628E-4
84-85	0.0	0.0	0.0	1.4220538574951012	7.382362060564899E-4
86-87	0.0	0.0	0.0	1.5234031423551422	8.43698521207417E-4
88-89	0.0	0.0	0.0	1.6410990860635768	8.43698521207417E-4
90-91	0.0	0.0	0.0	1.7716614322204247	8.43698521207417E-4
92-93	0.0	0.0	0.0	1.901274617540914	8.43698521207417E-4
94-95	0.0	0.0	0.0	2.0265638479402157	8.43698521207417E-4
96-97	0.0	0.0	0.0	2.1628211591152136	8.43698521207417E-4
98-99	0.0	0.0	0.0	2.3134213451507373	8.43698521207417E-4
100-101	0.0	0.0	0.0	2.4696110338892603	8.43698521207417E-4
102-103	0.0	0.0	0.0	2.637296114979234	8.43698521207417E-4
104-105	0.0	0.0	0.0	2.8175312115721693	8.43698521207417E-4
106-107	0.0	0.0	0.0	2.992598654722708	8.43698521207417E-4
108-109	0.0	0.0	0.0	3.1637639922126626	8.43698521207417E-4
110-111	0.0	0.0	0.0	3.362982305532764	0.0010546231515092713
112-113	0.0	0.0	0.0	3.56504810136194	0.0010546231515092713
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AATCCGA	90	2.3908829E-4	33.055553	1
ATCAACG	2410	0.0	31.35477	2
TCAACGC	2420	0.0	30.73347	3
CAACGCA	2470	0.0	30.352228	4
AATCAAC	685	0.0	28.664234	1
GTAATAC	105	5.9004885E-4	28.333336	3
AGTATCA	315	0.0	28.333332	1
AACGCAG	2730	0.0	27.24359	5
TATCAAC	2225	0.0	25.404495	1
GTATCAA	1495	0.0	23.879597	1
ACGCAGA	3280	0.0	22.856707	6
CGCAGAG	3310	0.0	22.649546	7
ATACATG	425	0.0	22.4	1
GGTATCA	1120	0.0	22.3125	1
AGAGTAC	3360	0.0	21.958334	5
GCAGAGT	3510	0.0	21.52849	8
CAGAGTA	3540	0.0	21.177967	4
ATATATC	345	1.2769306E-9	20.695652	3
AGTACAT	2725	0.0	19.433027	7
GAGTACA	2740	0.0	19.326643	6
>>END_MODULE