FastQCFastQC Report
Sun 1 Jan 2017
ERR1142906_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1142906_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences168636
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA3960.2348253042055077No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT3060.18145591688607415No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA2700.16010816195830072No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG2440.1446903389549088No Hit
GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC2310.13698142745321285No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC2310.13698142745321285No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC2300.13638843426077468No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA2100.12452857041201167No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT2040.12097061125738277No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC2030.12037761806494461No Hit
ATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGT1920.11385469294812496No Hit
GTACATGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA1900.11266870656324865No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT1870.1108897269859342No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1820.10792476102374346No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG1820.10792476102374346No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA1730.1025878222918001No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA1720.10199482909936193No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTAAA401.9574236E-674.4833451
GTACAAA453.9399038E-666.207421
GTACAAG801.18294556E-437.2416731
TATTCTC1352.3155262E-630.8509275
GTAAAAA2005.966103E-826.8140051
GCCCGGC450.00953476226.43580420-21
TAGGCTT900.00953528126.4279616
CATGGGG9300.025.5982324
GGGCTCT701.0560947E-425.49166920-21
AGGGCTA2151.2193777E-724.906235
GGGCTAT2752.0991138E-923.7851626
TCTCTGC1751.7476908E-523.7851628
ATGGGGG7350.023.4754875
TGGGGGA4100.023.2050386
TCGGAGA650.002044220722.89412792-93
AGATACC650.002049523622.88393274-75
AGTCCTA650.00205306522.87713828-29
ATTCTCT2352.89554E-722.7730276
GCTATTG2653.7030986E-822.4388338
CAGATTA1350.002542371422.0429214
Produced by FastQC (version 0.11.5)