FastQCFastQC Report
Sun 1 Jan 2017
ERR1142904_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1142904_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences139323
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6100.437831513820403No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA3150.22609332271053595No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT2180.15647093444729154No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA1910.13709150678638846No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG1810.129913940986054No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC1680.12058310544561918No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA1660.11914759228555227No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC1660.11914759228555227No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT1590.11412329622531815No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT1470.10551021726491677No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG1440.10335694752481644No Hit
ATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGT1400.10048592120468264No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTATA350.00775213251.0495071
CGTTAAC558.461975E-443.314731
GTAAAAA1452.6066118E-936.966881
ATGGGGA902.383769E-433.051965
TATTCTC1102.028492E-532.4510155
GGCGTCG953.2806018E-431.3011328
CAAGCCC400.00534874129.75746280-81
GTATCGG850.007154947628.0271781
ATACGGG1107.7055267E-427.0522373
CGGGCGT1107.7379873E-427.0327976
TATCGGC900.00948912726.4510752
TAGTACT900.00948912726.4510754
GAGTACT907.2866715E-726.43206812-13
TAAGTAC900.00952246226.4320686
TCAGGGT450.00953853726.43206812-13
CATGGGG8100.025.7163244
TACGGGC1200.001280306524.7978844
ATGGGGG6800.024.4973355
TGGGGGG4200.024.0720636
TGGCGAA650.00205386122.87390314-15
Produced by FastQC (version 0.11.5)