##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1142904_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	139323
Sequences flagged as poor quality	0
Sequence length	125
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.80920594589551	33.0	33.0	33.0	27.0	33.0
2	30.87043058217236	33.0	33.0	33.0	27.0	33.0
3	31.00080388736964	33.0	33.0	33.0	27.0	33.0
4	30.870021460921745	33.0	33.0	33.0	27.0	33.0
5	31.13498848000689	33.0	33.0	33.0	27.0	33.0
6	34.36294796982551	37.0	37.0	37.0	27.0	37.0
7	34.40955190456709	37.0	37.0	37.0	27.0	37.0
8	34.24976493472004	37.0	37.0	37.0	27.0	37.0
9	33.96950970048018	37.0	37.0	37.0	14.0	37.0
10-11	34.19679449911357	37.0	37.0	37.0	27.0	37.0
12-13	34.13502795661879	37.0	37.0	37.0	24.5	37.0
14-15	34.047174551222696	37.0	37.0	37.0	20.5	37.0
16-17	34.13245479927937	37.0	37.0	37.0	27.0	37.0
18-19	34.14331804511818	37.0	37.0	37.0	27.0	37.0
20-21	34.1030052468006	37.0	37.0	37.0	27.0	37.0
22-23	34.2681682134321	37.0	37.0	37.0	27.0	37.0
24-25	34.44709057370284	37.0	37.0	37.0	27.0	37.0
26-27	34.53555766097486	37.0	37.0	37.0	27.0	37.0
28-29	34.69239824006087	37.0	37.0	37.0	27.0	37.0
30-31	34.75868306022696	37.0	37.0	37.0	27.0	37.0
32-33	34.81383906461963	37.0	37.0	37.0	27.0	37.0
34-35	34.838515535841175	37.0	37.0	37.0	27.0	37.0
36-37	34.88129382801117	37.0	37.0	37.0	27.0	37.0
38-39	34.84633908256354	37.0	37.0	37.0	27.0	37.0
40-41	34.80622725608837	37.0	37.0	37.0	27.0	37.0
42-43	34.80449028516469	37.0	37.0	37.0	27.0	37.0
44-45	34.78341336319201	37.0	37.0	37.0	27.0	37.0
46-47	34.76887879244633	37.0	37.0	37.0	27.0	37.0
48-49	34.746850843005106	37.0	37.0	37.0	27.0	37.0
50-51	34.68972818558314	37.0	37.0	37.0	27.0	37.0
52-53	34.645198567357866	37.0	37.0	37.0	27.0	37.0
54-55	34.67053896341595	37.0	37.0	37.0	27.0	37.0
56-57	34.613753651586606	37.0	37.0	37.0	27.0	37.0
58-59	34.511125226990515	37.0	37.0	37.0	27.0	37.0
60-61	34.493708863576	37.0	37.0	37.0	27.0	37.0
62-63	34.46511344142748	37.0	37.0	37.0	27.0	37.0
64-65	34.40231332945744	37.0	37.0	37.0	27.0	37.0
66-67	34.368851517696285	37.0	37.0	37.0	27.0	37.0
68-69	34.27803736640756	37.0	37.0	37.0	27.0	37.0
70-71	34.1760226236874	37.0	37.0	37.0	27.0	37.0
72-73	34.1116326808926	37.0	37.0	37.0	27.0	37.0
74-75	34.06578597934296	37.0	37.0	37.0	27.0	37.0
76-77	33.99179963107312	37.0	37.0	37.0	24.5	37.0
78-79	33.942539996985424	37.0	37.0	37.0	22.0	37.0
80-81	33.87402654263833	37.0	37.0	37.0	22.0	37.0
82-83	33.78721029550039	37.0	37.0	37.0	22.0	37.0
84-85	33.70246836487874	37.0	37.0	37.0	22.0	37.0
86-87	33.611686512636105	37.0	37.0	37.0	18.0	37.0
88-89	33.563733195524065	37.0	37.0	37.0	14.0	37.0
90-91	33.457781557962434	37.0	33.0	37.0	14.0	37.0
92-93	33.4227586256397	37.0	33.0	37.0	14.0	37.0
94-95	33.357439188073755	37.0	33.0	37.0	14.0	37.0
96-97	33.28507496967478	37.0	33.0	37.0	14.0	37.0
98-99	33.176654249477835	37.0	33.0	37.0	14.0	37.0
100-101	33.09986865054586	37.0	33.0	37.0	14.0	37.0
102-103	33.05095353961657	37.0	33.0	37.0	14.0	37.0
104-105	32.90005239623034	37.0	33.0	37.0	14.0	37.0
106-107	32.84494304601537	37.0	33.0	37.0	14.0	37.0
108-109	32.72549758474911	37.0	33.0	37.0	14.0	37.0
110-111	32.63407692915025	37.0	33.0	37.0	14.0	37.0
112-113	32.48798116606734	37.0	33.0	37.0	14.0	37.0
114-115	32.399280807906806	37.0	33.0	37.0	14.0	37.0
116-117	32.27486488232381	37.0	33.0	37.0	14.0	37.0
118-119	32.114058698133114	37.0	33.0	37.0	14.0	37.0
120-121	32.03068050501353	37.0	33.0	37.0	14.0	37.0
122-123	31.845599793286105	37.0	30.0	37.0	14.0	37.0
124-125	30.051682062545307	35.0	24.5	37.0	14.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	90.0
1	10.0
2	6.0
3	8.0
4	3.0
5	5.0
6	5.0
7	3.0
8	2.0
9	4.0
10	4.0
11	4.0
12	1.0
13	2.0
14	6.0
15	80.0
16	144.0
17	240.0
18	485.0
19	937.0
20	1649.0
21	2066.0
22	2058.0
23	1985.0
24	1777.0
25	1698.0
26	1835.0
27	2094.0
28	2572.0
29	3035.0
30	3674.0
31	4379.0
32	5284.0
33	6784.0
34	9798.0
35	19009.0
36	67587.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.7726854778461	22.93905522671111	12.424573300385035	21.863685995057754
2	15.659628900355003	26.319760840507637	37.2227891400894	20.79782111904796
3	18.109607947438793	33.18717023448395	28.110758083298588	20.592463734778672
4	13.005283778440745	24.470723554149743	34.549441069695554	27.97455159771396
5	12.732410163495967	42.64699538415081	30.54513035100585	14.075464101347368
6	24.6482718976791	41.18416325357476	18.71883308184235	15.448731766903787
7	22.536243336829553	39.6226957283869	21.292690986939466	16.548369947844076
8	23.560258316631828	38.45297358647788	19.762371685738707	18.224396411151577
9	25.420088076610845	22.571607146705748	19.917814320710935	32.09049045597247
10-11	23.42829023695525	32.44817838631931	23.942285933902525	20.181245442822917
12-13	24.446998814612595	31.701928948597292	24.042889471604585	19.80818276518553
14-15	23.620061641916504	30.584233175995575	23.396627655523705	22.399077526564216
16-17	21.650621452690565	32.17292908973346	24.65299231266614	21.523457144909834
18-19	22.1071047517091	31.1381182125498	26.378146520909745	20.376630514831355
20-21	24.848310671241	28.819715122231603	25.764374113124855	20.567600093402547
22-23	25.368930783268436	28.5847714568994	25.084778641530036	20.961519118302128
24-25	23.45791640028446	29.291866303668534	25.705224443470705	21.544992852576307
26-27	22.603276097420792	29.22408218981249	25.91924707234715	22.25339464041957
28-29	22.46994032971336	28.943861073490368	26.73667118115554	21.84952741564073
30-31	23.522797488180945	28.60571050854277	26.45564800045983	21.415844002816456
32-33	22.810338943473496	29.41066585238805	26.154868607763188	21.624126596375262
34-35	22.321579334997935	29.38331926206686	26.398893459555588	21.89620794337962
36-37	22.645596539881886	29.772390901383762	25.931128130702803	21.650884428031556
38-39	22.649974854515413	29.41411020906674	26.245419929592646	21.690495006825202
40-41	21.678834214762013	29.43852034950563	26.485255231087606	22.397390204644747
42-43	22.212641200310426	29.571584605213992	25.879178523181285	22.3365956712943
44-45	22.413563901454044	29.841876641504417	26.085144452388736	21.6594150046528
46-47	22.009089765929545	29.58449350602691	26.040203352075736	22.36621337596781
48-49	22.26844716524938	29.69042461083029	26.297561694132188	21.743566529788144
50-51	21.778620907706017	29.88524331005145	26.16768704550027	22.16844873674226
52-53	21.34051031811338	30.00571332066102	25.670057528467787	22.98371883275781
54-55	21.25591020795309	29.58804593075895	25.633416207981835	23.522627653306124
56-57	22.139272203400793	28.90277243871858	25.322708705321055	23.635246652559573
58-59	21.865388484865907	29.31027048779787	26.120684699186526	22.7036563281497
60-61	22.231722958529286	29.281349136490853	26.195925142181714	22.29100276279815
62-63	21.63886832857851	28.836853338315716	26.25703342268086	23.267244910424917
64-65	20.8845023461696	28.867586930434097	26.49375193118861	23.7541587922077
66-67	22.126577185065962	28.17628545971891	26.08678469807145	23.61035265714368
68-69	21.651266059264795	28.127784324433076	26.196002069381773	24.02494754692036
70-71	21.696750643116854	28.187560179929005	26.332221950763834	23.783467226190304
72-73	22.15835195550797	28.292532208577935	26.497618038240727	23.051497797673363
74-75	22.367262758117658	27.83484781522961	26.725305681013822	23.0725837456389
76-77	21.67435643137424	27.58828856021733	26.97167004441378	23.765684963994655
78-79	21.60543288837786	27.79720091266776	27.091141014354754	23.50622518459963
80-81	21.5089810892288	28.01402767447459	27.15023337537324	23.32675786092337
82-83	22.008458710783238	27.62124810900741	27.52350939477457	22.846783785434777
84-85	22.041505704788428	28.28856347138622	26.450094331147245	23.219836492678105
86-87	21.271389250217396	28.470610217970922	26.861161217992485	23.3968393138192
88-89	22.10280843969185	27.420015522594	27.014703345981374	23.46247269173278
90-91	22.326834965411912	27.412092354685115	27.040876830473454	23.22019584942952
92-93	21.83820068060212	27.864799502664557	27.165152021503292	23.131847795230033
94-95	20.727088483872823	28.291913413442188	27.416201920312773	23.56479618237222
96-97	20.741440836025575	28.635241439039206	26.79637329346783	23.826944431467393
98-99	20.67089103501817	29.27825121213973	26.245116397761542	23.805741355080563
100-101	21.04637165597657	29.862915251801148	26.0302915970463	23.06042149517598
102-103	20.92114861489922	29.844798280886724	26.482034475695603	22.75201862851845
104-105	21.132075471698116	30.177538185085357	26.171428571428574	22.51895777178796
106-107	20.849146110056928	30.11830832039561	26.42854062446093	22.60400494508654
108-109	20.80856721888813	30.299708915801197	26.095878103999716	22.795845761310957
110-111	20.35590308971604	31.44229870848576	25.91149857336908	22.290299628429125
112-113	20.22713795396144	31.469388488562238	25.41285557691962	22.890617980556705
114-115	20.501421388756114	31.521407085020968	25.329111694920737	22.648059831302177
116-117	20.622057713731266	31.08240198368491	25.310310130448844	22.985230172134976
118-119	20.201457566697332	30.76701931922723	26.029927552897885	23.001595561177552
120-121	20.70944258595847	30.648609608951393	26.31304729018372	22.328900514906415
122-123	20.57909355832079	31.034111039644696	26.298522801180034	22.088272600854484
124-125	20.381199524129578	30.969316421483178	25.76816771916457	22.881316335222678
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	383.0
1	287.0
2	131.0
3	57.5
4	47.5
5	41.0
6	36.0
7	38.0
8	35.5
9	41.0
10	47.0
11	43.5
12	45.5
13	59.0
14	72.0
15	81.5
16	99.5
17	136.5
18	166.5
19	190.0
20	228.0
21	277.5
22	350.0
23	443.5
24	523.0
25	583.5
26	694.0
27	837.0
28	964.5
29	1206.5
30	1681.5
31	2101.5
32	2428.5
33	2800.5
34	3114.0
35	3463.5
36	3707.0
37	4180.5
38	4456.5
39	4217.5
40	3999.5
41	3794.0
42	3747.5
43	3903.0
44	4284.5
45	4778.5
46	5285.0
47	5869.5
48	6199.5
49	6183.0
50	6413.5
51	6278.0
52	5612.0
53	5285.0
54	4979.5
55	4552.5
56	4147.5
57	3339.5
58	2502.0
59	2050.0
60	1597.5
61	1183.0
62	882.0
63	688.5
64	504.5
65	286.5
66	179.5
67	140.0
68	112.0
69	86.0
70	76.0
71	64.5
72	52.5
73	41.5
74	22.5
75	17.0
76	20.5
77	19.0
78	14.0
79	6.5
80	5.0
81	5.5
82	3.5
83	3.0
84	1.5
85	0.0
86	0.5
87	0.5
88	0.0
89	0.0
90	0.0
91	0.5
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.08254200670384647
2	0.12130086202565263
3	0.15001112522699053
4	0.15647093444729154
5	0.17010830946792704
6	0.10981675674511746
7	0.08971957250418093
8	0.081824250123813
9	0.09043732908421437
10-11	0.08577191131399697
12-13	0.09187284224428127
14-15	0.09474386856441507
16-17	0.09546162514444852
18-19	0.10156255607473282
20-21	0.10084479949469938
22-23	0.0983326514645823
24-25	0.08038873696374611
26-27	0.09546162514444852
28-29	0.10156255607473282
30-31	0.10120367778471609
32-33	0.10084479949469938
34-35	0.10802236529503385
36-37	0.0983326514645823
38-39	0.09546162514444852
40-41	0.11125226990518436
42-43	0.11412329622531815
44-45	0.11448217451533486
46-47	0.11161114819520107
48-49	0.10658685213496696
50-51	0.11412329622531815
52-53	0.1252485232158366
54-55	0.11268778306525126
56-57	0.1073046087150004
58-59	0.11986534886558574
60-61	0.10945787845510074
62-63	0.11914759228555227
64-65	0.11484105280535159
66-67	0.10838124358505057
68-69	0.10838124358505057
70-71	0.11268778306525126
72-73	0.109099000165084
74-75	0.119506470575569
76-77	0.1270429146659202
78-79	0.12237749689570279
80-81	0.12165974031566934
82-83	0.12668403637590347
84-85	0.1331438455962045
86-87	0.12632515808588674
88-89	0.12273637518571952
90-91	0.1331438455962045
92-93	0.1302728192760707
94-95	0.1284784278259871
96-97	0.13529711533630484
98-99	0.15037000351700724
100-101	0.12596627979587002
102-103	0.1302728192760707
104-105	0.1421158028466226
106-107	0.13996253310652226
108-109	0.13493823704628813
110-111	0.13206721072615432
112-113	0.14391019429670623
114-115	0.14247468113663933
116-117	0.13493823704628813
118-119	0.13422048046625468
120-121	0.12309525347573623
122-123	0.1252485232158366
124-125	0.15037000351700724
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	139323.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.68084953668813
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.06401690665896	44.326493113125615
2	10.302823464552459	11.267342793365058
3	2.8562802725017393	4.685514954458346
4	1.2824383392620402	2.8049927147707128
5	0.7902025386241025	2.160447305900677
6	0.5408030663008808	1.7742942658426821
7	0.4069149285273617	1.5575317786725809
8	0.33340771563209637	1.4584813706279653
9	0.2599005027368311	1.2790422256196035
>10	1.9938831497840723	20.562290504798202
>50	0.13257550897181786	4.914479303489014
>100	0.03544097764593151	2.7712581555091407
>500	0.001312628801701167	0.437831513820403
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	610	0.437831513820403	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	315	0.22609332271053595	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	218	0.15647093444729154	No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA	191	0.13709150678638846	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG	181	0.129913940986054	No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC	168	0.12058310544561918	No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA	166	0.11914759228555227	No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC	166	0.11914759228555227	No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT	159	0.11412329622531815	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	147	0.10551021726491677	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG	144	0.10335694752481644	No Hit
ATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGT	140	0.10048592120468264	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	7.177565800334474E-4	0.0
20-21	0.0	0.0	0.0	0.0017943914500836186	0.0
22-23	0.0	0.0	0.0	0.0039476611901839605	0.0
24-25	0.0	0.0	0.0	0.015072888180702397	0.0
26-27	0.0	0.0	0.0	0.02619811517122083	0.0
28-29	0.0	0.0	0.0	0.057420526402675795	0.0
30-31	0.0	0.0	0.0	0.11053451332515091	0.0
32-33	0.0	0.0	0.0	0.17764475355827825	0.0
34-35	0.0	0.0	0.0	0.23578303654098748	0.0
36-37	0.0	0.0	0.0	0.3330390531355196	0.0
38-39	0.0	0.0	0.0	0.47838476059229273	0.0
40-41	0.0	0.0	0.0	0.6258837377891662	0.0
42-43	0.0	0.0	0.0	0.7482612346848689	0.0
44-45	0.0	0.0	0.0	0.8595135045900533	0.0
46-47	0.0	0.0	0.0	0.9811732449057227	0.0
48-49	0.0	0.0	0.0	1.1240068043323785	0.0
50-51	0.0	0.0	0.0	1.2783244690395699	0.0
52-53	0.0	0.0	0.0	1.4215169067562428	0.0
54-55	0.0	0.0	0.0	1.5489186997121798	0.0
56-57	0.0	0.0	0.0	1.686010206498568	0.0
58-59	0.0	0.0	0.0	1.8385334797556756	0.0
60-61	0.0	0.0	0.0	1.9975165622330842	0.0
62-63	0.0	0.0	0.0	2.1313781644093224	0.0
64-65	0.0	0.0	0.0	2.2623687402654262	0.0
66-67	0.0	0.0	0.0	2.4177630398426677	0.0
68-69	0.0	0.0	0.0	2.604379750651364	0.0
70-71	0.0	0.0	0.0	2.797097392390345	0.0
72-73	0.0	0.0	0.0	2.9794075637188406	0.0
74-75	0.0	0.0	0.0	3.1448504554165497	0.0
76-77	0.0	0.0	0.0	3.3275195050350623	0.0
78-79	0.0	0.0	0.0	3.5345922783747117	0.0
80-81	0.0	0.0	0.0	3.747765982644646	0.0
82-83	0.0	0.0	0.0	3.974577061935215	0.0
84-85	0.0	0.0	0.0	4.17447226947453	0.0
86-87	0.0	0.0	0.0	4.388004852034481	0.0
88-89	0.0	0.0	0.0	4.628453306345686	0.0
90-91	0.0	0.0	0.0	4.880026987647409	0.0
92-93	0.0	0.0	0.0	5.110426849838146	0.0
94-95	0.0	0.0	0.0	5.34226222518895	0.0
96-97	0.0	0.0	0.0	5.618239630211811	0.0
98-99	0.0	0.0	0.0	5.900317966164955	0.0
100-101	0.0	0.0	0.0	6.1648112659072805	0.0
102-103	0.0	0.0	0.0	6.45837370714096	0.0
104-105	0.0	0.0	0.0	6.775622115515744	0.0
106-107	0.0	0.0	0.0	7.113326586421481	0.0
108-109	0.0	0.0	0.0	7.454978718517402	0.0
110-111	0.0	0.0	0.0	7.787300015072889	0.0
112-113	0.0	0.0	0.0	8.125722242558659	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCTATA	35	0.007752132	51.049507	1
CGTTAAC	55	8.461975E-4	43.31473	1
GTAAAAA	145	2.6066118E-9	36.96688	1
ATGGGGA	90	2.383769E-4	33.05196	5
TATTCTC	110	2.028492E-5	32.451015	5
GGCGTCG	95	3.2806018E-4	31.301132	8
CAAGCCC	40	0.005348741	29.757462	80-81
GTATCGG	85	0.0071549476	28.027178	1
ATACGGG	110	7.7055267E-4	27.052237	3
CGGGCGT	110	7.7379873E-4	27.032797	6
TATCGGC	90	0.009489127	26.451075	2
TAGTACT	90	0.009489127	26.451075	4
GAGTACT	90	7.2866715E-7	26.432068	12-13
TAAGTAC	90	0.009522462	26.432068	6
TCAGGGT	45	0.009538537	26.432068	12-13
CATGGGG	810	0.0	25.716324	4
TACGGGC	120	0.0012803065	24.797884	4
ATGGGGG	680	0.0	24.497335	5
TGGGGGG	420	0.0	24.072063	6
TGGCGAA	65	0.002053861	22.873903	14-15
>>END_MODULE