FastQCFastQC Report
Sun 1 Jan 2017
ERR1142904_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1142904_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences139323
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10620.7622574879955212No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10010.7184743366134808No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8290.595020204847728No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3400.24403723721137216No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT2930.2103026779498001No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA2780.19953632924929837No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA2120.15216439496709086No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG2110.1514466383870574No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2010.14426907258672295No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1880.13493823704628813No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1870.13422048046625468No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1800.12919618440602054No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA1740.12488964492581986No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG1680.12058310544561918No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT1660.11914759228555227No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC1640.11771207912548538No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG1520.109099000165084No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC1520.109099000165084No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC1520.109099000165084No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT1440.10335694752481644No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC1440.10335694752481644No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC1420.10192143436474953No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGAGTA250.002053043371.45
GTAGACC300.00422875159.53
TAATACG350.00778196450.9999969
TTATACG255.3256063E-447.644-45
TATTCTC909.641735E-846.2777755
TCTCTGC1053.2362732E-739.6666688
TTCTCTG1501.4625039E-731.7333327
GACACTC850.00718897628.0000028
TTTAAGG900.00950079226.4444432
CTCTATC450.00951681426.444443104-105
GGTATCA14550.026.171821
GTATCAA18850.025.883291
ATGGGGA1501.6762508E-423.85
CTCTGCA1751.7364653E-523.89
ATTCTCT2001.8067294E-623.86
AATAACG802.6043857E-422.31249842-43
TATCAAC22800.021.6600882
ATCAACG23650.020.3784353
CAACGCA25000.019.9925
CATGGGC1208.830413E-619.83333416-17
Produced by FastQC (version 0.11.5)