FastQCFastQC Report
Sun 1 Jan 2017
ERR1142903_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1142903_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences580824
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7830.13480847898847154No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26550.027.13851
CCCCGTA900.00951198726.451942
GTATCAA33300.023.9621351
CTATAGC1450.00384719820.5336881
GTCTTAC1754.7537184E-420.4163531
ATCAACG37700.020.3651893
TATCAAC39400.020.2417772
TCAACGC38000.020.0460574
CAACGCA38000.019.8894465
TTTAGAC1500.004695429519.8389573
TTACACC2408.842406E-619.8372444
CATGGGG21750.019.7004344
AACGCAG39050.019.64256
GTTCTAG1550.005658576319.2089351
GGTCTAG1600.00679716518.6086541
CTAGACA1600.006820907418.5974164
TATAGCA1959.881444E-418.3128832
CATGGGT11850.018.0795154
TATTAAG1650.00814140818.0354142
ACGCAGA42250.018.0124977
Produced by FastQC (version 0.11.5)