FastQCFastQC Report
Sun 1 Jan 2017
ERR1142903_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1142903_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences580824
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19780.3405506659504428No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17810.3066333347106869No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10130.1744073936338719No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26450.039.8288541
GTATCAA35450.030.716582
TATCAAC44650.024.5207843
ATCAACG44550.024.3086994
GAGTACT21250.023.65911112-13
CAACGCA45700.023.564766
TCAACGC46550.023.3900995
AACGCAG48200.022.3425187
TATACCC1400.003152918321.251033
GTACTTT24550.020.6000414-15
ACGCAGA53400.020.0554268
CGCAGAG55000.019.4719949
GTATACG1856.935304E-419.3032191
CAGAGTA56900.018.71722210-11
CATGGGG19650.018.4716324
TCTAGGG2301.4340991E-418.1095733
CATGGGT10300.017.9086344
ACTTTTT28600.017.78691916-17
GCAGAGT58450.017.7119089
GTCCTAC1700.0096676817.505371
Produced by FastQC (version 0.11.5)