##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1142903_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	580824
Sequences flagged as poor quality	0
Sequence length	125
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.41688876492707	33.0	14.0	33.0	14.0	33.0
2	27.079483630153025	27.0	27.0	33.0	14.0	33.0
3	30.122558640827513	33.0	27.0	33.0	27.0	33.0
4	31.77012657879151	33.0	33.0	33.0	27.0	33.0
5	32.21836563227415	33.0	33.0	33.0	33.0	33.0
6	35.729010853545994	37.0	37.0	37.0	33.0	37.0
7	35.87082661873476	37.0	37.0	37.0	33.0	37.0
8	36.11545321818657	37.0	37.0	37.0	37.0	37.0
9	36.19247999393964	37.0	37.0	37.0	37.0	37.0
10-11	36.18617429720535	37.0	37.0	37.0	37.0	37.0
12-13	36.21650964836164	37.0	37.0	37.0	37.0	37.0
14-15	36.240522946710186	37.0	37.0	37.0	37.0	37.0
16-17	36.23421811082187	37.0	37.0	37.0	37.0	37.0
18-19	36.21706317232071	37.0	37.0	37.0	37.0	37.0
20-21	36.13650778893434	37.0	37.0	37.0	37.0	37.0
22-23	36.177875742049224	37.0	37.0	37.0	37.0	37.0
24-25	36.20979677148327	37.0	37.0	37.0	37.0	37.0
26-27	36.146273225624284	37.0	37.0	37.0	37.0	37.0
28-29	36.15427220638266	37.0	37.0	37.0	37.0	37.0
30-31	36.12852086001956	37.0	37.0	37.0	37.0	37.0
32-33	36.09182730052477	37.0	37.0	37.0	37.0	37.0
34-35	36.04199809236533	37.0	37.0	37.0	37.0	37.0
36-37	35.98794213049048	37.0	37.0	37.0	37.0	37.0
38-39	35.94022716003471	37.0	37.0	37.0	37.0	37.0
40-41	35.8740324091291	37.0	37.0	37.0	35.0	37.0
42-43	35.80421177499552	37.0	37.0	37.0	33.0	37.0
44-45	35.71978861066347	37.0	37.0	37.0	33.0	37.0
46-47	35.61320641020343	37.0	37.0	37.0	33.0	37.0
48-49	35.41917947605471	37.0	37.0	37.0	33.0	37.0
50-51	35.368152831150226	37.0	37.0	37.0	33.0	37.0
52-53	35.35252761593874	37.0	37.0	37.0	33.0	37.0
54-55	35.28222146467777	37.0	37.0	37.0	33.0	37.0
56-57	35.25251453107998	37.0	37.0	37.0	33.0	37.0
58-59	35.22314332741071	37.0	37.0	37.0	33.0	37.0
60-61	35.177584776111175	37.0	37.0	37.0	33.0	37.0
62-63	35.16305369612826	37.0	37.0	37.0	33.0	37.0
64-65	35.13852991611917	37.0	37.0	37.0	33.0	37.0
66-67	35.11178429266008	37.0	37.0	37.0	33.0	37.0
68-69	35.02743257854358	37.0	37.0	37.0	33.0	37.0
70-71	35.01647745272234	37.0	37.0	37.0	33.0	37.0
72-73	35.008762551134254	37.0	37.0	37.0	33.0	37.0
74-75	34.94544388661626	37.0	37.0	37.0	27.0	37.0
76-77	34.90552731980772	37.0	37.0	37.0	27.0	37.0
78-79	34.80999063399584	37.0	37.0	37.0	27.0	37.0
80-81	34.72721943308128	37.0	37.0	37.0	27.0	37.0
82-83	34.74747341707643	37.0	37.0	37.0	27.0	37.0
84-85	34.70944554632729	37.0	37.0	37.0	27.0	37.0
86-87	34.64298307232484	37.0	37.0	37.0	27.0	37.0
88-89	34.575095037395144	37.0	37.0	37.0	27.0	37.0
90-91	34.523606979050456	37.0	37.0	37.0	27.0	37.0
92-93	34.42940460449293	37.0	37.0	37.0	27.0	37.0
94-95	34.37952460642122	37.0	37.0	37.0	27.0	37.0
96-97	34.32425226918998	37.0	37.0	37.0	27.0	37.0
98-99	34.24157920471606	37.0	37.0	37.0	27.0	37.0
100-101	34.17926859082958	37.0	37.0	37.0	27.0	37.0
102-103	34.098708903213364	37.0	37.0	37.0	24.5	37.0
104-105	33.95714278335606	37.0	37.0	37.0	22.0	37.0
106-107	33.89010268170737	37.0	35.0	37.0	22.0	37.0
108-109	33.822975634615645	37.0	33.0	37.0	22.0	37.0
110-111	33.774251752682396	37.0	33.0	37.0	22.0	37.0
112-113	33.69961726788149	37.0	33.0	37.0	22.0	37.0
114-115	33.568618893158686	37.0	33.0	37.0	22.0	37.0
116-117	33.512014827211	37.0	33.0	37.0	22.0	37.0
118-119	33.36418260953404	37.0	33.0	37.0	22.0	37.0
120-121	33.244315834056444	37.0	33.0	37.0	22.0	37.0
122-123	33.177515908433534	37.0	33.0	37.0	18.0	37.0
124-125	31.48448841645662	35.0	27.5	37.0	14.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	6.0
15	163.0
16	76.0
17	19.0
18	62.0
19	303.0
20	1039.0
21	2334.0
22	4246.0
23	5667.0
24	5099.0
25	4039.0
26	3934.0
27	4586.0
28	5931.0
29	7717.0
30	10372.0
31	14198.0
32	20116.0
33	30459.0
34	52153.0
35	114752.0
36	293553.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	40.086497174460085	22.676486444125615	12.197100570619552	25.03991581079475
2	17.04957886545044	21.04174844872351	33.85691066988974	28.051762015936312
3	19.794256396129608	24.764126579663234	29.725043903446853	25.716573120760305
4	15.84504132231405	17.857093663911847	33.10519972451791	33.1926652892562
5	16.627870540310667	34.23712238341362	31.23857207298722	17.896435003288488
6	30.881299670812503	34.21587262234343	17.9737751883531	16.929052518490973
7	26.633196975331597	31.357691830916078	22.3802735424156	19.628837651336724
8	26.401285070864837	31.38713276310896	20.99792708290291	21.213655083123285
9	26.596008429403746	15.742462432681844	20.694392793686212	36.9671363442282
10-11	25.8727256449458	24.83454540445987	26.238413013236368	23.054315937357963
12-13	26.83219729382131	23.673123640927724	26.32773721245345	23.166941852797514
14-15	25.044807109911282	24.022550759870047	24.74901992517514	26.183622205043534
16-17	24.16540983154966	25.989542443149734	25.682564770050824	24.162482955249782
18-19	24.213186782915304	24.809839125105025	27.76520942660772	23.211764665371952
20-21	25.516961751588052	23.736724621936347	27.498465535452805	23.247848091022803
22-23	25.097189510075342	23.692547139925345	27.526324669779484	23.683938680219825
24-25	24.198505219336077	24.49317431704432	27.548160276574023	23.760160187045578
26-27	24.12569804268272	24.435518374437542	27.54706488493433	23.891718697945407
28-29	23.743509221382038	24.38613073839924	28.038786276049198	23.831573764169526
30-31	24.110244232502527	24.1401158191142	27.923821428171745	23.825818520211527
32-33	23.859465173615416	24.24477983003457	27.58641171852403	24.309343277825985
34-35	24.10274024489346	24.250289244246108	27.69470614161949	23.952264369240943
36-37	24.07480420024689	24.48258763857492	27.32915191030658	24.11345625087161
38-39	24.13450546120684	24.216716251394573	27.448159855653348	24.200618431745244
40-41	23.958193444418043	24.4106587605756	27.629136838045653	24.0020109569607
42-43	24.228637640038357	24.33977304617758	27.446227163869196	23.985362149914863
44-45	24.14578254342107	24.321739459801936	27.14083784416622	24.391640152610776
46-47	24.122970125201437	24.461885183807833	27.09986157596794	24.315283115022794
48-49	24.339731140586476	24.69792914893324	26.749669435147304	24.212670275332975
50-51	24.028576692038712	24.814272162087246	26.69823681224745	24.458914333626595
52-53	24.13652398931857	24.602932745200327	26.619733101936742	24.640810163544362
54-55	24.274995523600953	24.67976529895459	26.6745175819181	24.370721595526355
56-57	24.301251325702793	24.495802515047586	26.430553833863613	24.772392325386004
58-59	24.15155021142377	24.518614933267223	26.682093026458965	24.647741828850048
60-61	24.170940053217542	24.721881948647052	26.82518871912035	24.281989279015054
62-63	23.88520062945274	24.652389200133605	26.75492663845378	24.707483531959877
64-65	23.85628004352437	24.420995000206602	26.95136564604768	24.771359310221342
66-67	24.221709158023774	24.38991846066967	26.89222552787075	24.496146853435807
68-69	24.26552621792488	24.276545046347948	26.79322824125725	24.664700494469926
70-71	23.764427778466455	24.384753384846356	27.243967191438372	24.60685164524882
72-73	24.15060328085616	24.45344889329642	27.147896781124746	24.248051044722672
74-75	24.1157390190488	24.334566064763163	27.32006597523518	24.229628940952853
76-77	23.988247730810023	24.355140283459363	27.28253309091911	24.37407889481151
78-79	24.07200804374475	24.425299230059363	27.225028580086224	24.277664146109665
80-81	23.807986584576398	24.516548902937895	27.42130146137212	24.25416305111359
82-83	23.972494249548916	24.566305800035813	27.265660509896282	24.19553944051899
84-85	23.912751539192595	24.623810310868695	26.979945732270018	24.483492417668693
86-87	23.834070217484125	24.579218489594094	27.132057215266585	24.454654077655196
88-89	23.84534729969836	24.279299753453714	27.225717256862662	24.649635689985264
90-91	24.181593735796042	24.37029117254108	26.967721719488175	24.480393372174703
92-93	23.958548544825973	24.60736815283115	26.879140669118357	24.55494263322452
94-95	23.508412186824234	24.713080038014958	27.089187085933087	24.689320689227717
96-97	23.80505970827652	24.58937647204661	26.815954574879825	24.78960924479705
98-99	23.647520238558457	24.68665098773807	26.70646084342535	24.959367930278123
100-101	23.74753044174425	24.804824193277636	26.608042904673972	24.83960246030414
102-103	23.958052661436735	24.717319461075522	26.706650126931848	24.61797775055589
104-105	23.841076135594736	24.807728397314825	26.592535712502517	24.75865975458792
106-107	23.855070916735247	24.806825499034126	26.623561091005506	24.71454249322512
108-109	23.885221191019962	24.860047363949164	26.392790211794846	24.861941233236028
110-111	23.838718785725106	25.01420395851411	26.28722297976668	24.859854275994106
112-113	23.78127149437735	25.055245123062974	26.255788147973426	24.907695234586242
114-115	23.778623325643057	25.046658172927927	26.366258048965257	24.808460452463756
116-117	23.75245772370881	25.162356109100553	26.296782835360922	24.788403331829716
118-119	23.611841646515366	24.984827341603904	26.35058181226536	25.052749199615366
120-121	23.652377074441436	24.948564010474865	26.470724959066423	24.92833395601728
122-123	23.560301821171386	25.098332924419882	26.423729032424685	24.91763622198405
124-125	23.577382048955517	25.03258363707424	26.423737072000797	24.966297241969446
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	102.0
1	111.5
2	189.0
3	261.5
4	236.0
5	262.0
6	430.5
7	731.0
8	1018.0
9	1222.5
10	1195.0
11	1032.0
12	958.0
13	937.5
14	918.0
15	884.5
16	870.5
17	838.5
18	837.0
19	828.0
20	814.0
21	810.0
22	822.0
23	841.5
24	914.0
25	1046.5
26	1196.0
27	1471.5
28	1802.5
29	2350.0
30	3220.5
31	4046.5
32	5034.5
33	6186.5
34	7128.5
35	8035.0
36	8962.0
37	10143.5
38	11037.0
39	11399.0
40	11692.5
41	12068.0
42	12817.5
43	13912.0
44	15456.5
45	17057.0
46	18886.5
47	20758.0
48	22464.5
49	23833.0
50	24742.0
51	24585.5
52	23819.0
53	23894.0
54	23923.5
55	23528.0
56	22552.5
57	20923.0
58	19673.5
59	18156.0
60	15794.5
61	13239.5
62	10807.0
63	8473.0
64	6539.0
65	5271.5
66	4187.5
67	3244.0
68	2758.0
69	2430.5
70	2014.0
71	1721.5
72	1570.0
73	1499.5
74	1216.0
75	1059.5
76	878.5
77	619.0
78	483.5
79	339.5
80	234.5
81	175.0
82	140.5
83	113.0
84	78.0
85	46.0
86	33.0
87	17.5
88	9.5
89	7.0
90	5.5
91	2.5
92	1.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03908240706306902
2	0.0020660303293252346
3	6.886767764417448E-4
4	0.004132060658650469
5	0.0017216919411043622
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	8.608459705521811E-4
14-15	1.721691941104362E-4
16-17	0.0
18-19	0.0
20-21	4.3042298527609055E-4
22-23	0.0
24-25	5.165075823313086E-4
26-27	6.025921793865267E-4
28-29	0.0
30-31	7.747613734969631E-4
32-33	0.0
34-35	0.0
36-37	1.721691941104362E-4
38-39	0.0
40-41	0.0010330151646626173
42-43	1.721691941104362E-4
44-45	0.0
46-47	0.0
48-49	0.0
50-51	1.721691941104362E-4
52-53	5.165075823313086E-4
54-55	0.0
56-57	0.0
58-59	0.0
60-61	8.60845970552181E-5
62-63	3.443383882208724E-4
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	3.443383882208724E-4
100-101	2.582537911656543E-4
102-103	8.60845970552181E-5
104-105	8.608459705521811E-4
106-107	3.443383882208724E-4
108-109	4.3042298527609055E-4
110-111	0.0
112-113	6.025921793865267E-4
114-115	6.886767764417448E-4
116-117	3.443383882208724E-4
118-119	0.001635607344049144
120-121	8.608459705521811E-4
122-123	0.0028407917028221976
124-125	0.0018938611352147983
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	580824.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	78.15149207567981
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.28542469782172	68.21486176594162
2	8.980970984088785	14.0375256538985
3	1.9420478200000337	4.553218044459716
4	0.7178311438774626	2.2439829980966275
5	0.34294185758957507	1.3400708932915295
6	0.17331154223103704	0.8126733371575643
7	0.12270532577935722	0.6712723006702402
8	0.07682590679219573	0.4803247396701761
9	0.057717484962295874	0.4059636811793132
>10	0.2706872290829502	3.739210923912993
>50	0.019690673344818957	1.0792236077513178
>100	0.009068071185382639	1.455433003673944
>500	0.0	0.0
>1k	7.772632444611465E-4	0.9662390502964721
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1978	0.3405506659504428	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1781	0.3066333347106869	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1013	0.1744073936338719	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	1.721691941104362E-4	0.0
8	0.0	0.0	0.0	1.721691941104362E-4	0.0
9	0.0	0.0	0.0	1.721691941104362E-4	0.0
10-11	0.0	0.0	0.0	1.721691941104362E-4	0.0
12-13	0.0	0.0	0.0	1.721691941104362E-4	0.0
14-15	0.0	0.0	0.0	1.721691941104362E-4	8.60845970552181E-5
16-17	0.0	0.0	0.0	1.721691941104362E-4	1.721691941104362E-4
18-19	0.0	0.0	0.0	3.443383882208724E-4	1.721691941104362E-4
20-21	0.0	0.0	0.0	6.886767764417448E-4	1.721691941104362E-4
22-23	0.0	0.0	0.0	0.001721691941104362	1.721691941104362E-4
24-25	0.0	0.0	0.0	0.004390314449816123	5.165075823313086E-4
26-27	0.0	0.0	0.0	0.007575444540859193	5.165075823313086E-4
28-29	0.0	0.0	0.0	0.01764734239631971	5.165075823313086E-4
30-31	0.0	0.0	0.0	0.03667203834552291	5.165075823313086E-4
32-33	0.0	0.0	0.0	0.06637122432957315	5.165075823313086E-4
34-35	0.0	0.0	0.0	0.09753384846356211	6.886767764417448E-4
36-37	0.0	0.0	0.0	0.14703249177031252	6.886767764417448E-4
38-39	0.0	0.0	0.0	0.2168470999820944	6.886767764417448E-4
40-41	0.0	0.0	0.0	0.2891581615084776	6.886767764417448E-4
42-43	0.0	0.0	0.0	0.35294684792639425	6.886767764417448E-4
44-45	0.0	0.0	0.0	0.4219866947646791	7.747613734969629E-4
46-47	0.0	0.0	0.0	0.5060913460876272	8.60845970552181E-4
48-49	0.0	0.0	0.0	0.5922620277399006	8.60845970552181E-4
50-51	0.0	0.0	0.0	0.6773996942275112	8.60845970552181E-4
52-53	0.0	0.0	0.0	0.7636564604768399	8.60845970552181E-4
54-55	0.0	0.0	0.0	0.8444038125146344	8.60845970552181E-4
56-57	0.0	0.0	0.0	0.93737517733427	8.60845970552181E-4
58-59	0.0	0.0	0.0	1.0428288087269122	8.60845970552181E-4
60-61	0.0	0.0	0.0	1.1463024943872844	8.60845970552181E-4
62-63	0.0	0.0	0.0	1.2435780890596808	8.60845970552181E-4
64-65	0.0	0.0	0.0	1.3425753756731815	8.60845970552181E-4
66-67	0.0	0.0	0.0	1.4593060892800573	8.60845970552181E-4
68-69	0.0	0.0	0.0	1.5866252083247248	8.60845970552181E-4
70-71	0.0	0.0	0.0	1.728320455077614	0.0010330151646626173
72-73	0.0	0.0	0.0	1.8577916890486619	0.0013773535528834896
74-75	0.0	0.0	0.0	1.9805483244494029	0.001549522746993926
76-77	0.0	0.0	0.0	2.114409872870267	0.001549522746993926
78-79	0.0	0.0	0.0	2.273666377422421	0.001549522746993926
80-81	0.0	0.0	0.0	2.4396374805448815	0.001549522746993926
82-83	0.0	0.0	0.0	2.612409266834704	0.001549522746993926
84-85	0.0	0.0	0.0	2.775797832045508	0.001635607344049144
86-87	0.0	0.0	0.0	2.948913956723551	0.001721691941104362
88-89	0.0	0.0	0.0	3.1268508188366875	0.0018077765381595801
90-91	0.0	0.0	0.0	3.322779361734364	0.0018938611352147983
92-93	0.0	0.0	0.0	3.530501494428605	0.0018938611352147983
94-95	0.0	0.0	0.0	3.728754321446772	0.0018938611352147983
96-97	0.0	0.0	0.0	3.932688731870584	0.0020660303293252346
98-99	0.0	0.0	0.0	4.150568847017341	0.0020660303293252346
100-101	0.0	0.0	0.0	4.394532595071829	0.0020660303293252346
102-103	0.0	0.0	0.0	4.648740410175888	0.002238199523435671
104-105	0.0	0.0	0.0	4.922403344214427	0.0024103687175461067
106-107	0.0	0.0	0.0	5.1890934258914925	0.0024103687175461067
108-109	0.0	0.0	0.0	5.450962770133465	0.002496453314601325
110-111	0.0	0.0	0.0	5.742617384956544	0.0027547071057669793
112-113	0.0	0.0	0.0	6.051747172981832	0.0027547071057669793
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2645	0.0	39.828854	1
GTATCAA	3545	0.0	30.71658	2
TATCAAC	4465	0.0	24.520784	3
ATCAACG	4455	0.0	24.308699	4
GAGTACT	2125	0.0	23.659111	12-13
CAACGCA	4570	0.0	23.56476	6
TCAACGC	4655	0.0	23.390099	5
AACGCAG	4820	0.0	22.342518	7
TATACCC	140	0.0031529183	21.25103	3
GTACTTT	2455	0.0	20.60004	14-15
ACGCAGA	5340	0.0	20.055426	8
CGCAGAG	5500	0.0	19.471994	9
GTATACG	185	6.935304E-4	19.303219	1
CAGAGTA	5690	0.0	18.717222	10-11
CATGGGG	1965	0.0	18.471632	4
TCTAGGG	230	1.4340991E-4	18.109573	3
CATGGGT	1030	0.0	17.908634	4
ACTTTTT	2860	0.0	17.786919	16-17
GCAGAGT	5845	0.0	17.711908	9
GTCCTAC	170	0.00966768	17.50537	1
>>END_MODULE