FastQCFastQC Report
Sun 1 Jan 2017
ERR1142892_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1142892_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences358154
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA6860.19153771841163297No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5690.15887020667087343No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT4150.11587194335397621No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG4020.11224221982722515No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGGA300.001307855839.6686750-51
GTAACAT750.003900601831.7438163
GTCCTAA900.00945328626.4828131
GCCCATA1250.001617619823.8345341
GTACAAA3204.8930815E-1022.3448731
CGTTACA802.610269E-422.31206744-45
ATCCTAG1500.004660405719.8621121
CATGGGT3652.559318E-919.5681064
GTCTTGA2451.0433589E-519.4567621
GAACAAA2201.0076897E-418.9592881
CATGGGG12250.018.463244
TACAAAG2702.4326582E-517.6354522
GAAAAAA16300.016.4502141
GTATCAA28800.016.344861
GTACATG39900.015.8299541
TACAAAC2654.2133318E-415.7221732
ACATGGG36100.015.6630663
ATGGGGG7250.015.5982545
TACATGG40000.015.4751092
GTGCCAT2704.815585E-415.4483081
Produced by FastQC (version 0.11.5)