##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1142892_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	358154
Sequences flagged as poor quality	0
Sequence length	125
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.331977305851673	33.0	33.0	33.0	27.0	33.0
2	31.40629170691937	33.0	33.0	33.0	27.0	33.0
3	31.484685358812133	33.0	33.0	33.0	27.0	33.0
4	31.45055758137561	33.0	33.0	33.0	27.0	33.0
5	31.521920738006557	33.0	33.0	33.0	27.0	33.0
6	34.86926015066145	37.0	37.0	37.0	27.0	37.0
7	34.874746617376886	37.0	37.0	37.0	27.0	37.0
8	34.83926467385538	37.0	37.0	37.0	27.0	37.0
9	34.777819038737526	37.0	37.0	37.0	27.0	37.0
10-11	34.87996504297034	37.0	37.0	37.0	27.0	37.0
12-13	34.831177370628275	37.0	37.0	37.0	27.0	37.0
14-15	34.81428938389631	37.0	37.0	37.0	27.0	37.0
16-17	34.83637345946157	37.0	37.0	37.0	27.0	37.0
18-19	34.828381087465175	37.0	37.0	37.0	27.0	37.0
20-21	34.833056450577125	37.0	37.0	37.0	27.0	37.0
22-23	34.88784852326094	37.0	37.0	37.0	27.0	37.0
24-25	34.9721977138326	37.0	37.0	37.0	27.0	37.0
26-27	35.012539298737416	37.0	37.0	37.0	27.0	37.0
28-29	35.05822634955913	37.0	37.0	37.0	27.0	37.0
30-31	35.05280968521921	37.0	37.0	37.0	30.0	37.0
32-33	35.08779742792207	37.0	37.0	37.0	33.0	37.0
34-35	35.10013848791302	37.0	37.0	37.0	33.0	37.0
36-37	35.08062593186172	37.0	37.0	37.0	33.0	37.0
38-39	35.05701597636771	37.0	37.0	37.0	33.0	37.0
40-41	35.03640752302083	37.0	37.0	37.0	30.0	37.0
42-43	35.01957258609425	37.0	37.0	37.0	30.0	37.0
44-45	35.006405065977205	37.0	37.0	37.0	27.0	37.0
46-47	35.004107171775274	37.0	37.0	37.0	27.0	37.0
48-49	34.96302707773751	37.0	37.0	37.0	27.0	37.0
50-51	34.94784087292059	37.0	37.0	37.0	27.0	37.0
52-53	34.90235345689284	37.0	37.0	37.0	27.0	37.0
54-55	34.875970951043406	37.0	37.0	37.0	27.0	37.0
56-57	34.84412710733372	37.0	37.0	37.0	27.0	37.0
58-59	34.801764045634	37.0	37.0	37.0	27.0	37.0
60-61	34.76224612876025	37.0	37.0	37.0	27.0	37.0
62-63	34.74071069986654	37.0	37.0	37.0	27.0	37.0
64-65	34.685766458004096	37.0	37.0	37.0	27.0	37.0
66-67	34.657711487237336	37.0	37.0	37.0	27.0	37.0
68-69	34.58349899763789	37.0	37.0	37.0	27.0	37.0
70-71	34.50909245743451	37.0	37.0	37.0	27.0	37.0
72-73	34.46003814001798	37.0	37.0	37.0	27.0	37.0
74-75	34.4120894922296	37.0	37.0	37.0	27.0	37.0
76-77	34.33239751615227	37.0	37.0	37.0	27.0	37.0
78-79	34.28801018556264	37.0	37.0	37.0	27.0	37.0
80-81	34.21847445512266	37.0	37.0	37.0	27.0	37.0
82-83	34.132903722979506	37.0	37.0	37.0	27.0	37.0
84-85	34.06667802118642	37.0	37.0	37.0	27.0	37.0
86-87	33.99781658169391	37.0	37.0	37.0	27.0	37.0
88-89	33.9331349084472	37.0	37.0	37.0	22.0	37.0
90-91	33.839207435907454	37.0	37.0	37.0	22.0	37.0
92-93	33.7814655148344	37.0	37.0	37.0	22.0	37.0
94-95	33.73232185037721	37.0	33.0	37.0	22.0	37.0
96-97	33.63190275691463	37.0	33.0	37.0	22.0	37.0
98-99	33.52620939595816	37.0	33.0	37.0	22.0	37.0
100-101	33.43034560552165	37.0	33.0	37.0	22.0	37.0
102-103	33.38356684554689	37.0	33.0	37.0	22.0	37.0
104-105	33.22533742468323	37.0	33.0	37.0	14.0	37.0
106-107	33.175483172043315	37.0	33.0	37.0	14.0	37.0
108-109	33.075178554476565	37.0	33.0	37.0	14.0	37.0
110-111	32.94578728703295	37.0	33.0	37.0	14.0	37.0
112-113	32.81781719595482	37.0	33.0	37.0	14.0	37.0
114-115	32.689122556218834	37.0	33.0	37.0	14.0	37.0
116-117	32.594309989557566	37.0	33.0	37.0	14.0	37.0
118-119	32.486860400833166	37.0	33.0	37.0	14.0	37.0
120-121	32.40283369723639	37.0	33.0	37.0	14.0	37.0
122-123	32.204821110472025	37.0	33.0	37.0	14.0	37.0
124-125	30.223162382662206	35.0	27.5	37.0	14.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	202.0
1	27.0
2	21.0
3	16.0
4	12.0
5	12.0
6	16.0
7	7.0
8	10.0
9	20.0
10	8.0
11	9.0
12	12.0
13	5.0
14	26.0
15	109.0
16	202.0
17	424.0
18	909.0
19	1602.0
20	2579.0
21	3099.0
22	3283.0
23	3463.0
24	3762.0
25	4025.0
26	4675.0
27	5423.0
28	6363.0
29	7721.0
30	9198.0
31	11103.0
32	14183.0
33	19129.0
34	28927.0
35	54857.0
36	172715.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	44.13425932395232	19.443567206517333	11.936894764054049	24.485278705476293
2	17.300736284808604	23.298354680188517	35.55802785247469	23.842881182528192
3	19.826354421530933	27.576371897937786	27.87864383082838	24.7186298497029
4	14.730598536308442	19.849883525499944	33.30779190635059	32.11172603184102
5	15.555257203113682	37.636041720859595	30.8378016273261	15.97089944870063
6	29.916123280052098	36.07397697512766	17.97521989340026	16.034679851419977
7	26.58298590541764	33.14434372450177	21.39280405065555	18.87986631942504
8	25.399535086444864	34.310005699661374	20.324042512768358	19.966416701125404
9	26.42195887321897	18.954421869455025	19.561347867515767	35.06227138981024
10-11	24.875482874965947	27.692049764936815	25.341138502162025	22.091328857935217
12-13	25.95475414050471	26.292309712826274	24.884524138856058	22.868412007812957
14-15	24.560839700202884	26.67490121338468	23.1514187825912	25.612840303821237
16-17	24.120287052458615	28.408049317579724	23.94493131085054	23.526732319111122
18-19	24.1662486323641	27.523192170485334	25.403443857410945	22.907115339739622
20-21	25.56596998074542	26.365775478779447	24.987913491338233	23.080341049136898
22-23	25.426397747675367	26.210379974989696	24.563891547495125	23.799330729839806
24-25	24.61148799678126	26.731749295900574	24.71836023067638	23.93840247664178
26-27	24.357406851066504	26.62204553870231	24.88838895836681	24.132158651864376
28-29	24.277892830492416	26.373435958491587	25.267672236708616	24.080998974307377
30-31	24.81002434259619	26.143976255488266	25.21429030572177	23.83170909619378
32-33	24.009373847134185	26.730137170070766	25.133452951895453	24.1270360308996
34-35	24.102327596447463	26.656883913660216	25.140137936817393	24.100650553074928
36-37	24.377095155518354	26.81296016567012	24.69345577868171	24.116488900129816
38-39	24.31872360761386	26.62755958985079	24.854186869971297	24.199529932564047
40-41	24.04248093347365	26.5237498270491	25.21295671266112	24.220812526816125
42-43	24.25722232316315	26.634753805084628	25.034871207144754	24.073152664607473
44-45	24.122575911108004	26.638946154652505	25.170830567105767	24.06764736713372
46-47	24.049072552242308	26.61912029427878	25.185042319346145	24.14676483413276
48-49	24.05822743414405	26.769051023074912	25.2726965266743	23.90002501610674
50-51	23.805004856709598	26.85334134632183	25.287733838337957	24.05391995863062
52-53	23.81271866006371	26.740020435062767	25.008631088411697	24.438629816461827
54-55	23.894474398428045	26.642740747573168	25.051359668614843	24.411425185383948
56-57	24.123792021577657	26.278343383806973	24.867410611343644	24.730453983271726
58-59	24.00535026933043	26.3711812243004	25.246059277853405	24.377409228515763
60-61	24.072632545451242	26.48617071974308	25.194152166105095	24.247044568700588
62-63	23.63094065045082	26.677316963742864	25.312345818780024	24.37939656702629
64-65	23.600351632870687	26.243048752024755	25.459002486307142	24.69759712879742
66-67	24.15689431323799	26.126318761101768	25.150271333917505	24.56651559174273
68-69	24.10820223352978	26.019881238042398	25.16669019172691	24.70522633670091
70-71	23.990702978904206	26.02383235173264	25.305451587565585	24.680013081797572
72-73	24.237685145586834	25.913813488968827	25.48000190072088	24.36849946472346
74-75	24.23446995745347	25.795161660041483	25.548464467217926	24.421903915287118
76-77	24.153452170606784	25.87939768737	25.452209747042115	24.5149403949811
78-79	24.213146811701773	25.81334138462678	25.61625243041432	24.357259373257126
80-81	23.976718538367024	25.97261185403239	25.548245154238288	24.502424453362295
82-83	24.243597575039175	25.807213657388317	25.64115108758403	24.30803767998848
84-85	24.072135043511693	25.992381085520567	25.34106874497606	24.59441512599168
86-87	23.902912241366295	25.966530331537584	25.50999868603874	24.620558741057373
88-89	24.270549984484248	25.442897839256812	25.595539291195717	24.691012885063223
90-91	24.25700015516294	25.680026056191352	25.52038994823708	24.542583840408625
92-93	23.934808013822334	26.095805771607182	25.637577512986397	24.33180870158409
94-95	23.489976040906587	26.149798849851692	25.691025544251634	24.669199564990087
96-97	23.734315238542575	26.11093404012615	25.36653693887137	24.78821378245991
98-99	23.678076704199867	26.467269491001677	25.19110333072796	24.66355047407049
100-101	23.74027106816962	26.717659688674182	25.068633700125247	24.473435543030952
102-103	23.783297175191787	26.586158887074767	25.33758471069759	24.29295922703585
104-105	23.68112380065624	26.830908432559237	25.302990268241977	24.184977498542544
106-107	23.604847210533233	26.762412309275298	25.43010046162578	24.20264001856569
108-109	23.70243520563648	26.731763916459307	25.184947018201136	24.380853859703077
110-111	23.3630659079059	27.261150414396884	25.223623348248548	24.15216032944867
112-113	23.45921340334748	27.227227227227228	24.986718562137554	24.326840807287734
114-115	23.377776286559886	27.216031337943992	25.092304057464084	24.31388831803204
116-117	23.519659128081393	27.28820620067045	25.065493148731655	24.126641522516504
118-119	23.428070572743017	26.861788754268908	25.257811943015067	24.452328729973004
120-121	23.39817061579577	27.087969226984537	25.50137539276968	24.012484764450008
122-123	23.30144176002348	27.333198219290093	25.398598075323747	23.966761945362677
124-125	23.395033506563596	27.11997349115603	25.056310321251956	24.42868268102842
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	376.0
1	315.0
2	167.5
3	80.0
4	68.5
5	59.5
6	54.5
7	59.5
8	60.5
9	58.0
10	64.5
11	66.0
12	73.5
13	75.5
14	84.5
15	117.0
16	150.0
17	158.5
18	185.0
19	240.0
20	289.0
21	351.5
22	473.5
23	565.5
24	655.0
25	797.5
26	999.0
27	1260.5
28	1480.0
29	1931.5
30	2606.5
31	3094.5
32	3760.5
33	4447.5
34	5043.5
35	5725.0
36	6256.5
37	6996.0
38	7578.5
39	7899.5
40	8179.5
41	8258.0
42	8578.5
43	9408.0
44	10395.5
45	11289.0
46	12303.0
47	13264.0
48	14531.0
49	15683.5
50	16544.0
51	16198.5
52	14845.5
53	14425.0
54	14257.5
55	13729.5
56	13096.5
57	12036.5
58	10726.5
59	9625.0
60	8495.5
61	7417.0
62	6225.0
63	4876.0
64	3733.5
65	2889.5
66	2273.5
67	1769.5
68	1442.0
69	1251.0
70	1070.5
71	910.0
72	800.0
73	753.0
74	610.5
75	497.5
76	386.5
77	263.5
78	194.5
79	137.0
80	86.0
81	63.5
82	45.0
83	23.0
84	12.0
85	8.5
86	6.0
87	2.5
88	0.0
89	0.5
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0946520211975854
2	0.1150343148478029
3	0.14770182658856246
4	0.15775336866264233
5	0.17729803380668652
6	0.10246988725520308
7	0.07985391758852337
8	0.06645186148975021
9	0.07957470808646559
10-11	0.07580537980868565
12-13	0.08013312709058114
14-15	0.0871133646420255
16-17	0.08655494563790996
18-19	0.09172032142597876
20-21	0.08850941215231437
22-23	0.08418166487041887
24-25	0.0698023755144435
26-27	0.09116190242186323
28-29	0.09688569721404759
30-31	0.09632727820993203
32-33	0.09772332572022091
34-35	0.1065184250350408
36-37	0.09339557843832541
38-39	0.09297676418523874
40-41	0.10986893905973408
42-43	0.11419668634162958
44-45	0.11629075760706288
46-47	0.11112538181899406
48-49	0.10735605354121411
50-51	0.11210261507619629
52-53	0.12159573814616059
54-55	0.10693723928812744
56-57	0.10512237752475191
58-59	0.11587194335397621
60-61	0.10623921553298303
62-63	0.11656996710912065
64-65	0.11014814856179185
66-67	0.10735605354121411
68-69	0.10596000603092526
70-71	0.11307984833339847
72-73	0.11028775331282073
74-75	0.11950166688072729
76-77	0.1301116279589227
78-79	0.12494625217085388
80-81	0.12410862366468056
82-83	0.12773834719143162
84-85	0.13723147026139593
86-87	0.12759874244040273
88-89	0.12648190443217164
90-91	0.1299720232078938
92-93	0.13178688497126934
94-95	0.12871558044863382
96-97	0.1345789799918471
98-99	0.1562177164013246
100-101	0.1278779519424605
102-103	0.1301116279589227
104-105	0.14253645080049365
106-107	0.14100079853917588
108-109	0.13513739899596264
110-111	0.1299720232078938
112-113	0.1429552650535803
114-115	0.140861193788147
116-117	0.13485818949390485
118-119	0.1341601657387604
120-121	0.12229376190130503
122-123	0.12047890013792949
124-125	0.15007510735605353
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	358154.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	76.8561083322668
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.93321566062963	66.81348640485663
2	9.003726119320904	13.839827000411752
3	1.9836521726929988	4.573673588340886
4	0.7206018859958832	2.2153062645814146
5	0.3846884175069933	1.4782827345042877
6	0.22192529182669687	1.0233788562181523
7	0.15219225943559012	0.8187833344959918
8	0.11505667292584298	0.7074246494991021
9	0.07196483569980665	0.49778434877923095
>10	0.3798120044558122	5.2647566798677445
>50	0.026613631705459634	1.4063050310144496
>100	0.005732166828829342	0.9660676762569013
>500	8.18880975547049E-4	0.3949234311734239
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	686	0.19153771841163297	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	569	0.15887020667087343	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	415	0.11587194335397621	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG	402	0.11224221982722515	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	2.7920950205777403E-4	0.0
2	0.0	0.0	0.0	2.7920950205777403E-4	0.0
3	0.0	0.0	0.0	2.7920950205777403E-4	0.0
4	0.0	0.0	0.0	2.7920950205777403E-4	0.0
5	0.0	0.0	0.0	2.7920950205777403E-4	0.0
6	0.0	0.0	0.0	2.7920950205777403E-4	0.0
7	0.0	0.0	0.0	2.7920950205777403E-4	0.0
8	0.0	0.0	0.0	2.7920950205777403E-4	0.0
9	0.0	0.0	0.0	2.7920950205777403E-4	0.0
10-11	0.0	0.0	0.0	4.1881425308666105E-4	0.0
12-13	0.0	0.0	0.0	5.584190041155481E-4	0.0
14-15	0.0	0.0	0.0	5.584190041155481E-4	0.0
16-17	0.0	0.0	0.0	6.980237551444351E-4	0.0
18-19	0.0	0.0	0.0	8.376285061733221E-4	0.0
20-21	0.0	0.0	0.0	0.001675257012346644	5.584190041155481E-4
22-23	0.0	0.0	0.0	0.0037693282777799494	5.584190041155481E-4
24-25	0.0	0.0	0.0	0.010889170580253186	5.584190041155481E-4
26-27	0.0	0.0	0.0	0.019125850890957523	5.584190041155481E-4
28-29	0.0	0.0	0.0	0.032946721242817334	5.584190041155481E-4
30-31	0.0	0.0	0.0	0.05709834317081479	5.584190041155481E-4
32-33	0.0	0.0	0.0	0.09800253522227868	5.584190041155481E-4
34-35	0.0	0.0	0.0	0.14351368405769588	5.584190041155481E-4
36-37	0.0	0.0	0.0	0.2043813555062906	5.584190041155481E-4
38-39	0.0	0.0	0.0	0.2810243638211496	5.584190041155481E-4
40-41	0.0	0.0	0.0	0.36897535696934836	5.584190041155481E-4
42-43	0.0	0.0	0.0	0.44296587501465845	5.584190041155481E-4
44-45	0.0	0.0	0.0	0.5144435075414486	5.584190041155481E-4
46-47	0.0	0.0	0.0	0.6060242242163985	5.584190041155481E-4
48-49	0.0	0.0	0.0	0.6994198026547239	5.584190041155481E-4
50-51	0.0	0.0	0.0	0.7822054200148539	5.584190041155481E-4
52-53	0.0	0.0	0.0	0.8716920654243705	5.584190041155481E-4
54-55	0.0	0.0	0.0	0.9645292248585804	5.584190041155481E-4
56-57	0.0	0.0	0.0	1.0542950797701547	5.584190041155481E-4
58-59	0.0	0.0	0.0	1.161232319058282	5.584190041155481E-4
60-61	0.0	0.0	0.0	1.269007186852583	5.584190041155481E-4
62-63	0.0	0.0	0.0	1.3670097220748616	5.584190041155481E-4
64-65	0.0	0.0	0.0	1.4664083048074292	5.584190041155481E-4
66-67	0.0	0.0	0.0	1.585351552684041	5.584190041155481E-4
68-69	0.0	0.0	0.0	1.7091809668466635	5.584190041155481E-4
70-71	0.0	0.0	0.0	1.8479480893693774	5.584190041155481E-4
72-73	0.0	0.0	0.0	1.9869944213941488	5.584190041155481E-4
74-75	0.0	0.0	0.0	2.102726759997096	5.584190041155481E-4
76-77	0.0	0.0	0.0	2.233815621213221	5.584190041155481E-4
78-79	0.0	0.0	0.0	2.3889164996063146	5.584190041155481E-4
80-81	0.0	0.0	0.0	2.5482055205302747	5.584190041155481E-4
82-83	0.0	0.0	0.0	2.7217342260591812	5.584190041155481E-4
84-85	0.0	0.0	0.0	2.8762766854481594	5.584190041155481E-4
86-87	0.0	0.0	0.0	3.0440815961848813	5.584190041155481E-4
88-89	0.0	0.0	0.0	3.22863907704507	9.77233257202209E-4
90-91	0.0	0.0	0.0	3.4161282576768652	0.00139604751028887
92-93	0.0	0.0	0.0	3.6230224987016757	0.001954466514404418
94-95	0.0	0.0	0.0	3.8172127073828577	0.001954466514404418
96-97	0.0	0.0	0.0	4.013915801582559	0.001954466514404418
98-99	0.0	0.0	0.0	4.24593889779257	0.002094071265433305
100-101	0.0	0.0	0.0	4.487036302819458	0.0022336760164621923
102-103	0.0	0.0	0.0	4.7256208223278255	0.0022336760164621923
104-105	0.0	0.0	0.0	4.975652931420562	0.0022336760164621923
106-107	0.0	0.0	0.0	5.234619744579147	0.0022336760164621923
108-109	0.0	0.0	0.0	5.506011380579304	0.0022336760164621923
110-111	0.0	0.0	0.0	5.7888506061638285	0.0022336760164621923
112-113	0.0	0.0	0.0	6.0941661966640055	0.0022336760164621923
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGCGGA	30	0.0013078558	39.66867	50-51
GTAACAT	75	0.0039006018	31.743816	3
GTCCTAA	90	0.009453286	26.482813	1
GCCCATA	125	0.0016176198	23.834534	1
GTACAAA	320	4.8930815E-10	22.344873	1
CGTTACA	80	2.610269E-4	22.312067	44-45
ATCCTAG	150	0.0046604057	19.862112	1
CATGGGT	365	2.559318E-9	19.568106	4
GTCTTGA	245	1.0433589E-5	19.456762	1
GAACAAA	220	1.0076897E-4	18.959288	1
CATGGGG	1225	0.0	18.46324	4
TACAAAG	270	2.4326582E-5	17.635452	2
GAAAAAA	1630	0.0	16.450214	1
GTATCAA	2880	0.0	16.34486	1
GTACATG	3990	0.0	15.829954	1
TACAAAC	265	4.2133318E-4	15.722173	2
ACATGGG	3610	0.0	15.663066	3
ATGGGGG	725	0.0	15.598254	5
TACATGG	4000	0.0	15.475109	2
GTGCCAT	270	4.815585E-4	15.448308	1
>>END_MODULE