FastQCFastQC Report
Sun 1 Jan 2017
ERR1142892_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1142892_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences358154
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11110.31020175678618694No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10840.30266310023062704No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6760.18874562339105524No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA5680.15859099716881564No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT5580.1557989021482379No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG3800.10609961078195412No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGATT350.00278838433.99976362-63
CTAGACC850.007204093527.9998044
TACCGTG1107.74108E-427.0452657
GTATCAA29650.022.8831251
AGTATCA2004.8867594E-520.830671
CACCGGC1450.00386241520.5170977
GGTATCA23450.020.3040331
CAACGCA35950.019.695275
TATCAAC35350.019.692932
ATCAACG35200.019.269753
TCAACGC36350.019.151174
AACGCAG37400.018.9316846
AGCACCG1908.3068217E-418.7893415
TTGTATC1600.006823977518.5936183
GCCGTCA1959.890684E-418.3075648
GAGTACT15500.017.84987412-13
AAGCCGT1700.00967812917.4998766
ACGCAGA40450.017.357117
CATGGGG11000.017.03851716-17
CGCAGAG41750.016.8166478
Produced by FastQC (version 0.11.5)