FastQCFastQC Report
Sun 1 Jan 2017
ERR1142880_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1142880_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences501527
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15020.29948537167490485No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA6820.13598470271789953No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT5320.10607604376234978No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGGCG1352.324061E-630.8569815
ATCGCAA900.00954405126.4329939
TGGGGGG7550.025.9927656
CATGGGG19650.025.4393424
CCGATAA1200.001290099624.7809339
TAGGAAC1250.001630664623.8039554
ATGGGGG11400.023.4907475
GTCTTAT1552.0973253E-423.0429941
GTATTAT1703.917007E-421.0097891
GTACTAG1703.917007E-421.0097891
GAAAAAA21250.020.7296581
GTATCAA34500.020.70531
TATTCTC2659.203068E-720.2109075
TACATGG48900.020.2017232
GTACATG48850.020.1066041
ACATGGG47200.020.0467643
GTATATA1805.759259E-419.8425791
GTCGACA750.004705038419.83365878-79
AACGCAG35900.019.2153856
GCGTTAT1550.00566849119.2024961
Produced by FastQC (version 0.11.5)