##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1142880_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	501527
Sequences flagged as poor quality	0
Sequence length	125
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.258490569799832	33.0	33.0	33.0	27.0	33.0
2	31.334263160308417	33.0	33.0	33.0	27.0	33.0
3	31.408135554018028	33.0	33.0	33.0	27.0	33.0
4	31.369677006422386	33.0	33.0	33.0	27.0	33.0
5	31.399406213424204	33.0	33.0	33.0	27.0	33.0
6	34.729845053207505	37.0	37.0	37.0	27.0	37.0
7	34.73848068000327	37.0	37.0	37.0	27.0	37.0
8	34.70750328496771	37.0	37.0	37.0	27.0	37.0
9	34.67808512801903	37.0	37.0	37.0	27.0	37.0
10-11	34.73986744482351	37.0	37.0	37.0	27.0	37.0
12-13	34.70085259617129	37.0	37.0	37.0	27.0	37.0
14-15	34.703787632570126	37.0	37.0	37.0	27.0	37.0
16-17	34.71749477096946	37.0	37.0	37.0	27.0	37.0
18-19	34.705351855433506	37.0	37.0	37.0	27.0	37.0
20-21	34.70531795895336	37.0	37.0	37.0	27.0	37.0
22-23	34.76273161763973	37.0	37.0	37.0	27.0	37.0
24-25	34.84102451114297	37.0	37.0	37.0	27.0	37.0
26-27	34.89164691033584	37.0	37.0	37.0	27.0	37.0
28-29	34.9585515834641	37.0	37.0	37.0	27.0	37.0
30-31	34.9583482045832	37.0	37.0	37.0	27.0	37.0
32-33	34.994415056417694	37.0	37.0	37.0	27.0	37.0
34-35	35.001688842275684	37.0	37.0	37.0	27.0	37.0
36-37	34.97875189172268	37.0	37.0	37.0	27.0	37.0
38-39	34.95343221800621	37.0	37.0	37.0	27.0	37.0
40-41	34.92053069924451	37.0	37.0	37.0	27.0	37.0
42-43	34.90224653906968	37.0	37.0	37.0	27.0	37.0
44-45	34.888421759945125	37.0	37.0	37.0	27.0	37.0
46-47	34.8704057807456	37.0	37.0	37.0	27.0	37.0
48-49	34.82394467296875	37.0	37.0	37.0	27.0	37.0
50-51	34.77389652002783	37.0	37.0	37.0	27.0	37.0
52-53	34.73424860476106	37.0	37.0	37.0	27.0	37.0
54-55	34.72430896043483	37.0	37.0	37.0	27.0	37.0
56-57	34.68099125271421	37.0	37.0	37.0	27.0	37.0
58-59	34.60133851218379	37.0	37.0	37.0	27.0	37.0
60-61	34.567016332121696	37.0	37.0	37.0	27.0	37.0
62-63	34.54700444841454	37.0	37.0	37.0	27.0	37.0
64-65	34.48984401637399	37.0	37.0	37.0	27.0	37.0
66-67	34.46289731161034	37.0	37.0	37.0	27.0	37.0
68-69	34.40567706225188	37.0	37.0	37.0	27.0	37.0
70-71	34.346856699639304	37.0	37.0	37.0	27.0	37.0
72-73	34.29812452769243	37.0	37.0	37.0	27.0	37.0
74-75	34.254909506367554	37.0	37.0	37.0	27.0	37.0
76-77	34.180982280116524	37.0	37.0	37.0	27.0	37.0
78-79	34.136034550482826	37.0	37.0	37.0	27.0	37.0
80-81	34.08042637784207	37.0	37.0	37.0	27.0	37.0
82-83	34.00698765968731	37.0	37.0	37.0	27.0	37.0
84-85	33.9248415339553	37.0	37.0	37.0	27.0	37.0
86-87	33.84766921820759	37.0	37.0	37.0	22.0	37.0
88-89	33.76281137406361	37.0	35.0	37.0	22.0	37.0
90-91	33.66274996161722	37.0	33.0	37.0	22.0	37.0
92-93	33.61060321777292	37.0	33.0	37.0	22.0	37.0
94-95	33.55707269997428	37.0	33.0	37.0	22.0	37.0
96-97	33.44808953456145	37.0	33.0	37.0	22.0	37.0
98-99	33.32487084444108	37.0	33.0	37.0	18.0	37.0
100-101	33.22126824677435	37.0	33.0	37.0	14.0	37.0
102-103	33.160789947500334	37.0	33.0	37.0	14.0	37.0
104-105	32.99584469031578	37.0	33.0	37.0	14.0	37.0
106-107	32.9226821287787	37.0	33.0	37.0	14.0	37.0
108-109	32.812962213400276	37.0	33.0	37.0	14.0	37.0
110-111	32.675140122067205	37.0	33.0	37.0	14.0	37.0
112-113	32.5378125205622	37.0	33.0	37.0	14.0	37.0
114-115	32.40664311193615	37.0	33.0	37.0	14.0	37.0
116-117	32.302964745666735	37.0	33.0	37.0	14.0	37.0
118-119	32.18116472293615	37.0	33.0	37.0	14.0	37.0
120-121	32.09259920203698	37.0	33.0	37.0	14.0	37.0
122-123	31.8998099803201	37.0	30.0	37.0	14.0	37.0
124-125	29.92497113814411	35.0	20.5	37.0	14.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	314.0
1	34.0
2	25.0
3	28.0
4	23.0
5	21.0
6	26.0
7	15.0
8	17.0
9	23.0
10	15.0
11	8.0
12	20.0
13	16.0
14	40.0
15	171.0
16	258.0
17	570.0
18	1214.0
19	2193.0
20	3551.0
21	4607.0
22	4949.0
23	5237.0
24	5330.0
25	6052.0
26	7127.0
27	8483.0
28	10042.0
29	11998.0
30	14467.0
31	17339.0
32	21500.0
33	28444.0
34	41714.0
35	77415.0
36	228241.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.84725456972991	19.63761477960121	12.06280971283396	24.45232093783491
2	17.27902129732406	23.21504148466385	35.39911520678448	24.10682201122761
3	19.864043662593435	27.523369991752354	27.93155852532906	24.681027820325152
4	14.726830417953254	19.65155249481703	33.394117576566174	32.227499510663534
5	15.507652847194755	37.79200076708257	30.714065977091398	15.986280408631275
6	30.038025849593293	35.84230750037427	18.006686960427167	16.112979689605268
7	26.457252334956493	33.36573002315	21.339307096671188	18.83771054522232
8	25.693583887581838	34.22152779024962	20.178237539635504	19.90665078253304
9	26.472419573720764	18.776442883371917	19.52243154785663	35.22870599505069
10-11	25.368662100069344	27.467733647307686	25.248227658014077	21.915376594608887
12-13	25.889181931667483	26.253107134909776	24.905576028635036	22.95213490478771
14-15	24.630695737994802	26.47035636626361	23.070651550214702	25.828296345526887
16-17	24.250292638093747	28.3166898016248	23.79833767591965	23.634679884361802
18-19	24.297713001441103	27.476417448094438	25.27574520065628	22.950124349808185
20-21	25.6748719115566	26.245806064017273	24.832492051231387	23.246829973194735
22-23	25.65305210046464	26.06031151702885	24.47987354101135	23.806762841495154
24-25	24.722426206478186	26.677615299883463	24.641209431522483	23.958749062115867
26-27	24.432047264525657	26.498972076405657	24.741023133270794	24.32795752579789
28-29	24.318514334302726	26.53268157149387	25.026397152899893	24.12240694130351
30-31	24.795528014283846	26.315132873107107	25.032061806180018	23.85727730642903
32-33	24.252948931757643	26.562691809060723	24.86923300328452	24.315126255897116
34-35	24.39008323194254	26.639611377993187	24.954511382883904	24.015794007180364
36-37	24.517691064797212	26.701916062127555	24.62038536810768	24.160007504967552
38-39	24.566251605505272	26.600640106345075	24.607368508244836	24.225739779904814
40-41	24.199023626794265	26.688266253839068	24.93484690144739	24.177863217919274
42-43	24.378752564096164	26.659113708618857	24.81486102723557	24.14727270004941
44-45	24.345456451105754	26.57832612135096	24.802531430767466	24.27368599677582
46-47	24.239438784778994	26.520573432911505	24.968307869829694	24.27167991247981
48-49	24.469688500634817	26.726182396013833	24.91495851732371	23.889170586027646
50-51	23.95716875359356	26.910276784003067	24.870232543282437	24.262321919120936
52-53	23.859463819710523	26.760396055419005	24.823246592218258	24.55689353265221
54-55	23.959103780694374	26.495503831400413	24.86861845001213	24.676773937893085
56-57	24.104776819266576	26.268862891642986	24.582668347471458	25.043691941618984
58-59	24.13313594404952	26.37732432370378	24.94482117668903	24.54471855555766
60-61	24.248600405031656	26.367979942049928	25.044690222647638	24.338729430270774
62-63	23.582648278753577	26.433473588587912	25.15240904621436	24.831469086444155
64-65	23.719202209585184	26.064540729494507	25.153196641702024	25.06306041921828
66-67	24.110623015552182	26.03994797639973	24.929405811032158	24.92002319701593
68-69	24.096415606400427	25.929984998068612	24.890929071706836	25.082670323824125
70-71	23.867480522851025	25.99052719314444	25.049435274975917	25.092557009028617
72-73	24.178608075573248	26.041887095293042	25.004990836823595	24.77451399231012
74-75	24.266712720694965	25.79538035502864	25.19820868464504	24.739698239631355
76-77	24.099982128933256	25.745418167986028	25.17773724340293	24.976862459677783
78-79	24.114006189477887	25.766197464310668	25.232904063092743	24.886892283118698
80-81	23.823281593796047	25.952096669209052	25.395034761461698	24.829586975533203
82-83	24.012311599679332	25.97555849956622	25.314154446082842	24.69797545467161
84-85	23.948491169073137	26.046466580865214	24.98110404308578	25.023938206975878
86-87	23.684100180305382	26.166643704187358	25.126543228364852	25.02271288714241
88-89	23.94830923806643	25.73136897256424	25.17788167312102	25.142440116248306
90-91	24.29314228207217	25.625888564080444	25.240111979041867	24.840857174805514
92-93	23.917857620674575	25.97650906868961	25.306666080924302	24.798967229711515
94-95	23.4013026792382	26.173289884068733	25.364207070888593	25.06120036580447
96-97	23.68779710466172	26.0471613243991	25.137163630844867	25.12787794009431
98-99	23.463154352161524	26.244191608753358	25.119912758038375	25.172741281046747
100-101	23.631782187311565	26.519864865404507	24.900865356585218	24.947487590698714
102-103	23.76014017781816	26.42731270935567	25.143946844251865	24.66860026857431
104-105	23.74157254006039	26.57026344798939	25.04822823638426	24.63993577556596
106-107	23.610661777921564	26.532425879994086	25.23904525683885	24.617867085245496
108-109	23.625845320670205	26.535156253900233	25.055938686606567	24.783059738822995
110-111	23.333246804075127	27.006613497765546	24.961361358163238	24.698778339996085
112-113	23.395366577933633	27.13503463783542	24.721369617356263	24.748229166874687
114-115	23.33560338870671	27.11364789244331	24.821957093956325	24.728791624893653
116-117	23.40876118662328	27.082037400739466	24.788948120427627	24.720253292209627
118-119	23.21678726656755	26.79284807807325	25.057886953943054	24.932477701416143
120-121	23.252475356253633	26.807923536000732	25.235555679792025	24.704045427953606
122-123	23.184079800658083	26.894486950132574	25.248678241702073	24.67275500750727
124-125	23.39527415002806	26.816931732618592	24.847261695290328	24.940532422063022
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	948.0
1	767.5
2	400.0
3	194.0
4	155.5
5	129.0
6	121.5
7	118.5
8	115.0
9	112.0
10	119.0
11	117.0
12	99.0
13	108.0
14	141.0
15	156.0
16	144.0
17	171.0
18	226.5
19	258.0
20	305.0
21	364.5
22	456.0
23	598.5
24	744.5
25	929.0
26	1157.5
27	1492.0
28	1895.5
29	2495.0
30	3309.5
31	4021.5
32	4805.0
33	5549.5
34	6420.5
35	7330.0
36	8123.0
37	9226.0
38	10120.0
39	10724.0
40	11058.0
41	11402.0
42	11964.0
43	13107.0
44	14645.0
45	15798.0
46	17347.5
47	18886.5
48	20541.0
49	22023.0
50	22688.0
51	22522.0
52	21686.0
53	21393.0
54	20971.5
55	20044.5
56	18987.0
57	17444.5
58	15834.5
59	13982.0
60	12094.0
61	10394.5
62	8540.5
63	6712.5
64	5140.5
65	4047.0
66	3233.0
67	2560.0
68	2147.5
69	1790.0
70	1489.5
71	1319.0
72	1159.0
73	1084.0
74	867.5
75	703.5
76	569.0
77	364.5
78	247.0
79	165.5
80	112.5
81	87.0
82	65.0
83	43.0
84	26.5
85	21.0
86	12.5
87	5.5
88	2.0
89	0.0
90	0.5
91	0.5
92	0.5
93	0.5
94	0.5
95	0.5
96	0.5
97	1.0
98	0.5
99	0.0
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.09132110534427858
2	0.12342306595656864
3	0.1551262444494514
4	0.1692830096884116
5	0.1852342944647048
6	0.11006386495642309
7	0.08912780368753825
8	0.07935764176205867
9	0.08912780368753825
10-11	0.0846415048442058
12-13	0.09221836511294507
14-15	0.09640557736672203
16-17	0.0960067952473147
18-19	0.10388274210560947
20-21	0.10089187621005449
22-23	0.09869857455331418
24-25	0.07955703282176234
26-27	0.1030851778667948
28-29	0.10487969740412781
30-31	0.1079702588295346
32-33	0.10777086776983093
34-35	0.11514833697886655
36-37	0.10478000187427595
38-39	0.10298548233694296
40-41	0.11793981181471785
42-43	0.12352276148642047
44-45	0.1245197167849388
46-47	0.12242611065805033
48-49	0.11863768052368068
50-51	0.12581575867301262
52-53	0.13718104907612153
54-55	0.11973433135205085
56-57	0.11494894591916288
58-59	0.1307008396357524
60-61	0.1153477280385702
62-63	0.13050144857604876
64-65	0.1233233704267168
66-67	0.11973433135205085
68-69	0.11793981181471785
70-71	0.12352276148642047
72-73	0.121429155359532
74-75	0.1327944457626409
76-77	0.14306308533737963
78-79	0.1349877474193812
80-81	0.13658287589701051
82-83	0.13927465520301
84-85	0.1512381187852299
86-87	0.14156765238960214
88-89	0.14007221944182469
90-91	0.14336217192693515
92-93	0.14715060206130479
94-95	0.14216582556871316
96-97	0.15083933666582258
98-99	0.16958209627796708
100-101	0.13817800437463984
102-103	0.14645273335234196
104-105	0.1561231997479697
106-107	0.15652198186737704
108-109	0.1506399456061189
110-111	0.14615364676278644
112-113	0.1543286802106367
114-115	0.16050980306145032
116-117	0.1518362919643409
118-119	0.15283324726285924
120-121	0.13757983119552886
122-123	0.13538652953878855
124-125	0.16629214379285662
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	501527.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	75.20924704699554
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.56159975892396	65.10232741052062
2	9.273545358006011	13.949127276635856
3	2.0583258099200594	4.6441540302445485
4	0.7561312845605989	2.2747225832192064
5	0.378175918889924	1.4221163055508423
6	0.20406867009629456	0.920871061429443
7	0.14053187268809797	0.739850743168326
8	0.0986207002019007	0.5933750884345946
9	0.06967302026746941	0.47160498544257906
>10	0.4091267580328958	5.8537181283200646
>50	0.036169555498491976	1.856594531439098
>100	0.013095873386689006	1.534615953241097
>500	6.236130184137618E-4	0.2846918959710714
>1k	3.118065092068809E-4	0.35223000638266
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1502	0.29948537167490485	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	682	0.13598470271789953	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	532	0.10607604376234978	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	1.9939105970366501E-4	0.0
14-15	0.0	0.0	0.0	1.9939105970366501E-4	0.0
16-17	0.0	0.0	0.0	1.9939105970366501E-4	0.0
18-19	0.0	0.0	0.0	1.9939105970366501E-4	0.0
20-21	0.0	0.0	0.0	4.984776492591625E-4	0.0
22-23	0.0	0.0	0.0	0.0022929971865921477	0.0
24-25	0.0	0.0	0.0	0.006878991559776443	0.0
26-27	0.0	0.0	0.0	0.013757983119552886	0.0
28-29	0.0	0.0	0.0	0.02482418693310629	0.0
30-31	0.0	0.0	0.0	0.04406542419450997	0.0
32-33	0.0	9.969552985183251E-5	0.0	0.06978687089628274	0.0
34-35	0.0	1.9939105970366501E-4	0.0	0.10288578680709115	0.0
36-37	0.0	1.9939105970366501E-4	0.0	0.15243446514345188	0.0
38-39	0.0	1.9939105970366501E-4	0.0	0.21843290590536502	9.969552985183251E-5
40-41	0.0	1.9939105970366501E-4	0.0	0.29639481024949804	1.9939105970366501E-4
42-43	0.0	1.9939105970366501E-4	0.0	0.360100253824819	1.9939105970366501E-4
44-45	0.0	1.9939105970366501E-4	0.0	0.42849138730317615	1.9939105970366501E-4
46-47	0.0	1.9939105970366501E-4	0.0	0.5132325876772337	1.9939105970366501E-4
48-49	0.0	1.9939105970366501E-4	0.0	0.598771352290106	1.9939105970366501E-4
50-51	0.0	1.9939105970366501E-4	0.0	0.682116815246238	1.9939105970366501E-4
52-53	0.0	1.9939105970366501E-4	0.0	0.7603778061799265	3.9878211940733003E-4
54-55	0.0	1.9939105970366501E-4	0.0	0.8409317943002073	5.98173179110995E-4
56-57	0.0	1.9939105970366501E-4	0.0	0.9295611203384863	5.98173179110995E-4
58-59	0.0	1.9939105970366501E-4	0.0	1.033842644563503	5.98173179110995E-4
60-61	0.0	1.9939105970366501E-4	0.0	1.1422116855124451	5.98173179110995E-4
62-63	0.0	1.9939105970366501E-4	0.0	1.2399133047672408	5.98173179110995E-4
64-65	0.0	1.9939105970366501E-4	0.0	1.3341255804772225	5.98173179110995E-4
66-67	0.0	1.9939105970366501E-4	0.0	1.438706191291795	5.98173179110995E-4
68-69	0.0	1.9939105970366501E-4	0.0	1.5543530059199206	5.98173179110995E-4
70-71	0.0	1.9939105970366501E-4	0.0	1.690935881816931	5.98173179110995E-4
72-73	0.0	1.9939105970366501E-4	0.0	1.8109692997585376	5.98173179110995E-4
74-75	0.0	1.9939105970366501E-4	0.0	1.9275133741553296	5.98173179110995E-4
76-77	0.0	1.9939105970366501E-4	0.0	2.055821521074638	5.98173179110995E-4
78-79	0.0	1.9939105970366501E-4	0.0	2.2060626845613496	5.98173179110995E-4
80-81	0.0	1.9939105970366501E-4	0.0	2.3599925826525787	5.98173179110995E-4
82-83	0.0	1.9939105970366501E-4	0.0	2.5365533660201747	5.98173179110995E-4
84-85	0.0	1.9939105970366501E-4	0.0	2.689087526693478	5.98173179110995E-4
86-87	0.0	1.9939105970366501E-4	0.0	2.844114075613078	5.98173179110995E-4
88-89	0.0	1.9939105970366501E-4	0.0	3.012400130002971	5.98173179110995E-4
90-91	0.0	1.9939105970366501E-4	0.0	3.193845994333306	5.98173179110995E-4
92-93	0.0	1.9939105970366501E-4	0.0	3.380775112805492	5.98173179110995E-4
94-95	0.0	1.9939105970366501E-4	0.0	3.5633176279641976	7.975642388146601E-4
96-97	0.0	1.9939105970366501E-4	0.0	3.763506251906677	7.975642388146601E-4
98-99	0.0	1.9939105970366501E-4	0.0	3.974960470722414	7.975642388146601E-4
100-101	0.0	1.9939105970366501E-4	0.0	4.212634613889183	7.975642388146601E-4
102-103	0.0	1.9939105970366501E-4	0.0	4.457387139675431	7.975642388146601E-4
104-105	0.0	1.9939105970366501E-4	0.0	4.696855802379533	7.975642388146601E-4
106-107	0.0	1.9939105970366501E-4	0.0	4.950880012442003	9.96955298518325E-4
108-109	0.0	1.9939105970366501E-4	0.0	5.210188085586618	0.00119634635822199
110-111	0.0	1.9939105970366501E-4	0.0	5.493024303776267	0.001395737417925655
112-113	0.0	1.9939105970366501E-4	0.0	5.777256259383842	0.001395737417925655
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATGGGCG	135	2.324061E-6	30.856981	5
ATCGCAA	90	0.009544051	26.432993	9
TGGGGGG	755	0.0	25.992765	6
CATGGGG	1965	0.0	25.439342	4
CCGATAA	120	0.0012900996	24.780933	9
TAGGAAC	125	0.0016306646	23.803955	4
ATGGGGG	1140	0.0	23.490747	5
GTCTTAT	155	2.0973253E-4	23.042994	1
GTATTAT	170	3.917007E-4	21.009789	1
GTACTAG	170	3.917007E-4	21.009789	1
GAAAAAA	2125	0.0	20.729658	1
GTATCAA	3450	0.0	20.7053	1
TATTCTC	265	9.203068E-7	20.210907	5
TACATGG	4890	0.0	20.201723	2
GTACATG	4885	0.0	20.106604	1
ACATGGG	4720	0.0	20.046764	3
GTATATA	180	5.759259E-4	19.842579	1
GTCGACA	75	0.0047050384	19.833658	78-79
AACGCAG	3590	0.0	19.215385	6
GCGTTAT	155	0.005668491	19.202496	1
>>END_MODULE