FastQCFastQC Report
Sun 1 Jan 2017
ERR1142880_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1142880_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences501527
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25270.5038612078711615No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24060.479734889647018No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11620.2316924113756587No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA8290.16529518849433827No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6060.120830982180421No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5800.1156468146281257No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT5160.10288578680709114No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCAACG1301.8189894E-1145.791411
GCGCAGA2950.034.304751
GGTATCA34450.033.3499681
ATGGGGG12800.029.7495775
GTATCAA44550.028.194351
TACCCCG850.00720705627.9996015
TATGTCG850.00720705627.9996015
ATTACAC1150.001002936525.8743573
GAGTACT24500.025.49963612-13
CAACGCA52800.024.5659395
ATCAACG51600.024.5655963
TCAACGC53150.024.40664
TATCAAC52150.024.3065152
TGGGGGG7850.024.254436
AACGCAG54650.023.8432126
CATGGGG21650.023.6371234
ACGCAGA57200.022.780277
TATTAGA2153.410485E-622.1436352
CGCAGAG59200.022.1111728
CATGGGT4900.021.859014
Produced by FastQC (version 0.11.5)