ERR1142867_2.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	ERR1142867_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	74116
Sequences flagged as poor quality	0
Sequence length	125
%GC	43

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	322	0.43445409897997733	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	316	0.4263586809865616	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG	224	0.3022289384208538	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	193	0.2604026121215392	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG	161	0.21722704948998867	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	137	0.18484537751632577	No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC	136	0.1834961411840898	No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA	134	0.1807976685196179	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC	130	0.17540072319067407	No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA	127	0.1713530141939662	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	115	0.15516217820713477	No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT	114	0.1538129418748988	No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC	111	0.14976523287819093	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC	111	0.14976523287819093	No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA	110	0.148415996545955	No Hit
GTACATGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA	108	0.14571752388148307	No Hit
ATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGT	107	0.14436828754924713	No Hit
GTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTAGGGCTGAA	104	0.14032057855253927	No Hit
GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC	102	0.13762210588806736	No Hit
GGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTAGGGCTGA	97	0.13087592422688757	No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC	95	0.12817745156241567	No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT	91	0.12278050623347185	No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA	87	0.11738356090452803	No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG	86	0.11603432457229208	No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC	86	0.11603432457229208	No Hit
GTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGC	83	0.11198661557558422	No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT	83	0.11198661557558422	No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT	83	0.11198661557558422	No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT	83	0.11198661557558422	No Hit
AATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGA	82	0.11063737924334827	No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT	81	0.10928814291111232	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	81	0.10928814291111232	No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC	80	0.10793890657887636	No Hit
ATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTC	79	0.1065896702466404	No Hit
GGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGA	78	0.10524043391440445	No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC	78	0.10524043391440445	No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG	77	0.1038911975821685	No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT	75	0.10119272491769658	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GACAGCG	20	8.450984E-4	89.24747	7
TTCTATC	25	0.0020439657	71.44624	2
CATGGGT	30	0.0042101075	59.53854	4
GTACAAA	30	0.0042101075	59.53854	1
GAAGCGA	15	0.0042297486	59.49831	74-75
TTCAGCG	15	0.0042297486	59.49831	82-83
AGCGCTA	15	0.0042297486	59.49831	84-85
GTTCTAC	30	0.0013050061	39.665543	20-21
GCGAAAT	30	0.0013050061	39.665543	42-43
GTGCGAA	30	0.0013050061	39.665543	40-41
GGCGTCG	80	1.186865E-4	37.186443	8
TACGGGC	70	0.002763441	34.022022	4
ACGGGCG	70	0.002763441	34.022022	5
AGGACGA	35	0.0027815679	33.999035	52-53
CCAGAAG	35	0.0027815679	33.999035	82-83
GACGACG	35	0.0027815679	33.999035	54-55
AAGTGGT	35	0.0027815679	33.999035	26-27
CGGGCGT	90	2.3719236E-4	33.054615	6
ATACGGG	75	0.0038747694	31.753887	3
GTAGGAT	100	4.382378E-4	29.76927	3

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content