##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1142855_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	423324
Sequences flagged as poor quality	0
Sequence length	125
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.354629078436375	33.0	33.0	33.0	27.0	33.0
2	31.393540172539236	33.0	33.0	33.0	27.0	33.0
3	31.456038400846634	33.0	33.0	33.0	27.0	33.0
4	31.381476127032723	33.0	33.0	33.0	27.0	33.0
5	31.508152148236338	33.0	33.0	33.0	27.0	33.0
6	34.89343623323979	37.0	37.0	37.0	27.0	37.0
7	34.8760429363797	37.0	37.0	37.0	27.0	37.0
8	34.854482618514425	37.0	37.0	37.0	27.0	37.0
9	34.69784136973099	37.0	37.0	37.0	27.0	37.0
10-11	34.79248282639303	37.0	37.0	37.0	27.0	37.0
12-13	34.74735781576287	37.0	37.0	37.0	27.0	37.0
14-15	34.753018964197636	37.0	37.0	37.0	27.0	37.0
16-17	34.78646970169421	37.0	37.0	37.0	27.0	37.0
18-19	34.77596710793624	37.0	37.0	37.0	27.0	37.0
20-21	34.781821961429074	37.0	37.0	37.0	27.0	37.0
22-23	34.842150456860466	37.0	37.0	37.0	27.0	37.0
24-25	34.93292371800323	37.0	37.0	37.0	27.0	37.0
26-27	35.016756668650956	37.0	37.0	37.0	30.0	37.0
28-29	35.11004455216336	37.0	37.0	37.0	33.0	37.0
30-31	35.11039889068421	37.0	37.0	37.0	33.0	37.0
32-33	35.182502055163425	37.0	37.0	37.0	33.0	37.0
34-35	35.19704292693067	37.0	37.0	37.0	33.0	37.0
36-37	35.18903133297427	37.0	37.0	37.0	33.0	37.0
38-39	35.177378320151945	37.0	37.0	37.0	33.0	37.0
40-41	35.158216874072814	37.0	37.0	37.0	33.0	37.0
42-43	35.15866097835228	37.0	37.0	37.0	33.0	37.0
44-45	35.15965430733905	37.0	37.0	37.0	33.0	37.0
46-47	35.16200239060389	37.0	37.0	37.0	33.0	37.0
48-49	35.138415256399355	37.0	37.0	37.0	33.0	37.0
50-51	35.127181544160024	37.0	37.0	37.0	33.0	37.0
52-53	35.12218182763084	37.0	37.0	37.0	33.0	37.0
54-55	35.1282563710066	37.0	37.0	37.0	33.0	37.0
56-57	35.10243336073551	37.0	37.0	37.0	33.0	37.0
58-59	35.05982061021818	37.0	37.0	37.0	33.0	37.0
60-61	35.05069875556312	37.0	37.0	37.0	33.0	37.0
62-63	35.02630491065945	37.0	37.0	37.0	33.0	37.0
64-65	34.986515057024874	37.0	37.0	37.0	27.0	37.0
66-67	34.95896996154246	37.0	37.0	37.0	27.0	37.0
68-69	34.88726129394979	37.0	37.0	37.0	27.0	37.0
70-71	34.83662395706362	37.0	37.0	37.0	27.0	37.0
72-73	34.78555787056723	37.0	37.0	37.0	27.0	37.0
74-75	34.7628116997855	37.0	37.0	37.0	27.0	37.0
76-77	34.675934981243685	37.0	37.0	37.0	27.0	37.0
78-79	34.65100726630193	37.0	37.0	37.0	27.0	37.0
80-81	34.60283376326407	37.0	37.0	37.0	27.0	37.0
82-83	34.53656301083804	37.0	37.0	37.0	27.0	37.0
84-85	34.48932614262361	37.0	37.0	37.0	27.0	37.0
86-87	34.43717105573981	37.0	37.0	37.0	27.0	37.0
88-89	34.37339602762896	37.0	37.0	37.0	27.0	37.0
90-91	34.29941014447563	37.0	37.0	37.0	27.0	37.0
92-93	34.2571788984324	37.0	37.0	37.0	27.0	37.0
94-95	34.198423666033584	37.0	37.0	37.0	27.0	37.0
96-97	34.096045818333	37.0	37.0	37.0	24.5	37.0
98-99	34.001024038325255	37.0	37.0	37.0	22.0	37.0
100-101	33.90838931881963	37.0	37.0	37.0	22.0	37.0
102-103	33.84428475588438	37.0	37.0	37.0	22.0	37.0
104-105	33.68774390301518	37.0	33.0	37.0	22.0	37.0
106-107	33.63753531573924	37.0	33.0	37.0	22.0	37.0
108-109	33.517944883824214	37.0	33.0	37.0	22.0	37.0
110-111	33.40287344917841	37.0	33.0	37.0	22.0	37.0
112-113	33.2712378698113	37.0	33.0	37.0	22.0	37.0
114-115	33.15369669567518	37.0	33.0	37.0	18.0	37.0
116-117	33.05693865691527	37.0	33.0	37.0	14.0	37.0
118-119	32.94983275221816	37.0	33.0	37.0	14.0	37.0
120-121	32.866039959936124	37.0	33.0	37.0	14.0	37.0
122-123	32.68862266254689	37.0	33.0	37.0	14.0	37.0
124-125	30.7071510238021	35.0	27.5	37.0	14.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	289.0
1	29.0
2	19.0
3	24.0
4	19.0
5	16.0
6	28.0
7	15.0
8	23.0
9	24.0
10	15.0
11	8.0
12	15.0
13	9.0
14	35.0
15	132.0
16	200.0
17	341.0
18	765.0
19	1378.0
20	2298.0
21	2969.0
22	3457.0
23	3792.0
24	4078.0
25	4438.0
26	4997.0
27	6059.0
28	7053.0
29	8600.0
30	10427.0
31	12559.0
32	15012.0
33	20268.0
34	30951.0
35	61171.0
36	221811.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.10998765671479	21.11482092777996	12.799892173600503	22.975299241904747
2	17.121473042787123	24.22648222091857	36.24841511647711	22.403629619817195
3	19.35073231904976	29.478030428507207	28.244800416439915	22.926436836003123
4	14.110898390077786	21.43541734210196	33.3568404537032	31.096843814117054
5	14.546956474400396	39.646663684326384	30.326140212598258	15.480239628674966
6	27.7367860943597	37.60506089629892	18.3287217689488	16.329431240392577
7	25.18519569386023	35.830430968645004	20.96961442883118	18.014758908663588
8	25.330222023627986	35.28948656297211	20.02255463114667	19.35773678225324
9	26.526361087072686	20.15846444126034	19.599275537081436	33.71589893458554
10-11	24.709625021722708	29.27298971850917	24.713408023984236	21.30397723578389
12-13	25.49247251824826	28.43914999869951	24.58177372865125	21.48660375440098
14-15	24.47906813968446	28.193574779614845	23.15403787219553	24.173319208505166
16-17	23.272671810599842	29.9627801831669	23.99247563804556	22.772072368187697
18-19	23.607506405701102	28.789177646320162	25.4849355173217	22.118380430657027
20-21	26.115400532072126	26.90830623706769	24.90948861956843	22.06680461129175
22-23	26.18956692610822	26.483611199457073	24.78366404939282	22.543157825041884
24-25	24.40391330765757	27.44954098073845	24.870734596316023	23.275811115287954
26-27	23.82100401767873	27.385954914030187	25.14513539269913	23.64790567559195
28-29	23.712845763802175	27.410650092708217	25.407726945926512	23.4687771975631
30-31	24.465327591059268	26.870791738504217	25.474719094480275	23.189161575956238
32-33	23.902000061489414	27.27741422817776	25.01897894460513	23.801606765727694
34-35	24.01221413045278	27.42289889282834	25.365726166007157	23.199160810711728
36-37	24.01815761032794	27.408579340811105	25.033877021092437	23.539386027768515
38-39	23.847529994903795	27.321109530616855	25.02048533391981	23.810875140559542
40-41	23.519873975966025	27.3934336463829	25.36434735610896	23.722345021542115
42-43	23.664546467372663	27.363107586118808	25.297983011597623	23.674362934910906
44-45	23.818118593830206	27.37943449473313	25.13181954737816	23.670627364058504
46-47	23.841816194941032	27.202831773004014	25.210632631145717	23.744719400909233
48-49	23.50441046211993	27.670431632811145	25.346882638779668	23.478275266289263
50-51	23.359730914974243	27.7284597153035	25.275447883699258	23.636361486023
52-53	23.513582884158406	27.45283811132441	25.12907495395883	23.904504050558348
54-55	23.115375245681797	27.28918668978224	25.441990714351604	24.153447350184365
56-57	23.588861794194376	27.01059846687654	25.121512215081943	24.27902752384714
58-59	23.510460132277387	27.22118141304039	25.423301825200827	23.84505662948139
60-61	23.65360349759759	27.28956290673349	25.597882709384596	23.45895088628432
62-63	23.332052017481093	27.168548229714307	25.48832918384123	24.011070568963376
64-65	23.297025604636033	27.034711134764354	25.59860445863639	24.06965880196322
66-67	23.7173498085279	26.945529203338875	25.331202974617824	24.005918013515398
68-69	23.449806758028885	26.947793919382384	25.305710217462263	24.29668910512647
70-71	23.5021400955385	27.20576495257965	25.373053128063933	23.91904182381792
72-73	23.731068624830584	27.43928832457773	25.164684928461106	23.66495812213058
74-75	23.865273476957725	27.163414339886877	25.43237533798729	23.538936845168113
76-77	23.587861839887292	26.80823831542987	25.393231578710783	24.210668265972057
78-79	23.67855140419069	27.12331943061138	25.37443902162059	23.823690143577345
80-81	23.50984564684841	27.178527535518732	25.598918380551666	23.71270843708119
82-83	23.704678075491294	26.972363547752522	25.753605821836384	23.569352554919803
84-85	23.52355916883953	27.410336978690985	25.070627818605995	23.995476033863493
86-87	23.20200628145905	27.57134660996398	25.425003992452787	23.801643116124186
88-89	23.41744825767012	26.792218038753667	25.612527221131998	24.17780648244422
90-91	23.843633180334077	26.549472389154406	25.573037429612455	24.03385700089907
92-93	23.66967506249253	27.13920840032131	25.420294072762246	23.770822464423915
94-95	23.089626128976924	27.408454536313577	25.4662889116278	24.0356304230817
96-97	23.091629695356403	27.190535344572613	25.47494824016563	24.242886719905353
98-99	22.770969711599903	27.51918170673407	25.302458003777172	24.40739057788885
100-101	23.16302111858845	27.75391574566846	25.12334850225168	23.959714633491412
102-103	23.243681274806136	27.64800870691644	25.456610335914252	23.65169968236317
104-105	23.326687924088525	27.710528339613937	25.440519643395902	23.522264092901636
106-107	23.248987530924232	27.744196419592264	25.30575205481235	23.701063994671152
108-109	23.01291068287137	27.92306354545121	25.220846854669933	23.843178917007485
110-111	22.764091067721118	28.403120767060408	25.188068212065467	23.644719953153004
112-113	22.778702615852236	28.52449085593468	25.031679154938573	23.665127373274505
114-115	22.74087351589259	28.707236705887922	24.93122970167001	23.620660076549477
116-117	22.83493817827325	28.58812686852339	25.03226844506911	23.544666508134252
118-119	22.559239239097128	28.152081196414354	25.456339417858903	23.832340146629612
120-121	22.874804972361968	28.13070370821618	25.471703692838716	23.52278762658314
122-123	22.838693498840197	28.56661260960749	25.258131329082108	23.336562562470206
124-125	22.88631272785792	28.526362000191696	24.872352233288645	23.714973038661743
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1715.0
1	1205.5
2	494.0
3	267.5
4	218.5
5	170.0
6	151.0
7	159.0
8	165.0
9	180.5
10	172.0
11	156.0
12	163.0
13	169.0
14	187.5
15	221.0
16	249.0
17	261.0
18	305.5
19	376.5
20	451.5
21	497.5
22	581.0
23	745.0
24	892.0
25	1062.5
26	1311.5
27	1694.0
28	2112.0
29	2725.0
30	3542.0
31	4323.0
32	5199.5
33	6148.5
34	7058.0
35	7896.0
36	8700.5
37	9663.5
38	10230.0
39	10471.5
40	10583.0
41	10543.0
42	10624.5
43	11224.0
44	12352.5
45	13337.5
46	14262.5
47	15363.0
48	16360.0
49	17004.0
50	17355.0
51	17062.0
52	16637.0
53	16735.5
54	16556.0
55	15899.0
56	15188.0
57	14014.5
58	12496.5
59	10884.5
60	9400.5
61	7612.0
62	5884.0
63	4682.5
64	3685.5
65	2987.0
66	2430.5
67	1901.5
68	1498.5
69	1234.0
70	991.0
71	844.0
72	736.0
73	705.0
74	596.0
75	486.5
76	419.5
77	341.0
78	269.0
79	179.5
80	115.5
81	95.5
82	85.0
83	74.0
84	61.0
85	45.0
86	26.5
87	9.0
88	2.0
89	2.5
90	3.5
91	2.5
92	1.0
93	0.5
94	0.5
95	0.0
96	0.5
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.09968723719893037
2	0.1370108947283877
3	0.16394062231293288
4	0.1788228401886026
5	0.19772089463389744
6	0.11197097258837203
7	0.09283669246251099
8	0.08291521387873119
9	0.09212801542081242
10-11	0.08964764577486747
12-13	0.09614385199043758
14-15	0.10110459128232747
16-17	0.10181326832402603
18-19	0.10830947453959615
20-21	0.1060653305742174
22-23	0.10122270412261057
24-25	0.0843325679621283
26-27	0.10464797649082026
28-29	0.11622303483856337
30-31	0.11693171188026193
32-33	0.11504190643573244
34-35	0.12638073910290934
36-37	0.11126229554667348
38-39	0.10878192590072853
40-41	0.12933356010998667
42-43	0.13346750951989492
44-45	0.138664474492351
46-47	0.12992412431140213
48-49	0.12342791809583203
50-51	0.13181392975593162
52-53	0.14279842390225927
54-55	0.12307357957498276
56-57	0.12449093365837988
58-59	0.13606599200612296
60-61	0.12271924105413348
62-63	0.137365233249237
64-65	0.12968789863083596
66-67	0.12921544726970358
68-69	0.12708941614460792
70-71	0.13287694531847946
72-73	0.12921544726970358
74-75	0.14197163402027763
76-77	0.15130254840264196
78-79	0.14764105035386607
80-81	0.14752293751358297
82-83	0.15106632272207576
84-85	0.16205081686840342
86-87	0.15390103088887
88-89	0.14846784023584772
90-91	0.1568538518959473
92-93	0.15909799586132609
94-95	0.15390103088887
96-97	0.16512175071576382
98-99	0.18567338492502197
100-101	0.15106632272207576
102-103	0.1586255445001937
104-105	0.17161795693133391
106-107	0.16972815148680445
108-109	0.16287760675038507
110-111	0.1586255445001937
112-113	0.1718541826119001
114-115	0.170436828528503
116-117	0.16476741219491453
118-119	0.16405873515321598
120-121	0.14823161455528153
122-123	0.14705048615245062
124-125	0.18295678959851083
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	423324.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	74.74065191775502
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.31612600980604	65.26064180905749
2	8.50009635674744	12.70605486134076
3	1.8906329378214248	4.23921414929861
4	0.734828005448138	2.1968609669846986
5	0.37418764316098985	1.3983514194710331
6	0.23338755031851285	1.0466122596176102
7	0.17721509152816833	0.9271620029335949
8	0.10962423238106893	0.6554709275315649
9	0.09046300456899273	0.6085137542332831
>10	0.5302618672915005	7.249953287822056
>50	0.029752425853227432	1.4757879100361329
>100	0.01306204061301033	1.5381604231820944
>500	0.0	0.0
>1k	3.628344614725092E-4	0.6972162284910375
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2571	0.6073362247356635	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	2.3622568056618572E-4	0.0
2	0.0	0.0	0.0	2.3622568056618572E-4	0.0
3	0.0	0.0	0.0	2.3622568056618572E-4	0.0
4	0.0	0.0	0.0	2.3622568056618572E-4	0.0
5	0.0	0.0	0.0	2.3622568056618572E-4	0.0
6	0.0	0.0	0.0	2.3622568056618572E-4	0.0
7	0.0	0.0	0.0	2.3622568056618572E-4	0.0
8	0.0	0.0	0.0	2.3622568056618572E-4	0.0
9	0.0	0.0	0.0	2.3622568056618572E-4	0.0
10-11	0.0	0.0	0.0	4.7245136113237144E-4	0.0
12-13	0.0	0.0	0.0	7.086770416985572E-4	0.0
14-15	0.0	0.0	0.0	7.086770416985572E-4	2.3622568056618572E-4
16-17	0.0	0.0	0.0	7.086770416985572E-4	2.3622568056618572E-4
18-19	0.0	0.0	0.0	0.0011811284028309286	2.3622568056618572E-4
20-21	0.0	0.0	0.0	0.0017716926042463928	2.3622568056618572E-4
22-23	0.0	0.0	0.0	0.004015836569625157	2.3622568056618572E-4
24-25	0.0	0.0	0.0	0.008504124500382685	2.3622568056618572E-4
26-27	0.0	0.0	0.0	0.014409766514537329	2.3622568056618572E-4
28-29	0.0	0.0	0.0	0.026221050542846614	2.3622568056618572E-4
30-31	0.0	0.0	0.0	0.046890797592387866	2.3622568056618572E-4
32-33	0.0	0.0	0.0	0.0876397274900549	2.3622568056618572E-4
34-35	0.0	0.0	0.0	0.1359478791658399	2.3622568056618572E-4
36-37	0.0	0.0	0.0	0.20445332653003373	2.3622568056618572E-4
38-39	0.0	0.0	0.0	0.3062665948540598	2.3622568056618572E-4
40-41	0.0	0.0	0.0	0.41410361803252355	2.3622568056618572E-4
42-43	0.0	0.0	0.0	0.5115467112660752	2.3622568056618572E-4
44-45	0.0	0.0	0.0	0.604265290888303	2.3622568056618572E-4
46-47	0.0	0.0	0.0	0.7332445124774405	2.3622568056618572E-4
48-49	0.0	0.0	0.0	0.8602158157817652	2.3622568056618572E-4
50-51	0.0	0.0	0.0	0.983407508197031	2.3622568056618572E-4
52-53	0.0	0.0	0.0	1.110378811501356	2.3622568056618572E-4
54-55	0.0	0.0	0.0	1.2308539085901107	2.3622568056618572E-4
56-57	0.0	0.0	0.0	1.3593606788181156	2.3622568056618572E-4
58-59	0.0	0.0	0.0	1.4987338303521653	2.3622568056618572E-4
60-61	0.0	0.0	0.0	1.650981281477072	2.3622568056618572E-4
62-63	0.0	0.0	0.0	1.7944883824210298	2.3622568056618572E-4
64-65	0.0	0.0	0.0	1.9326804055522484	2.3622568056618572E-4
66-67	0.0	0.0	0.0	2.083274276913192	2.3622568056618572E-4
68-69	0.0	0.0	0.0	2.2670578563936843	2.3622568056618572E-4
70-71	0.0	0.0	0.0	2.461235365819089	2.3622568056618572E-4
72-73	0.0	0.0	0.0	2.646081960862129	2.3622568056618572E-4
74-75	0.0	0.0	0.0	2.824196124009033	2.3622568056618572E-4
76-77	0.0	0.0	0.0	3.0076253649686766	2.3622568056618572E-4
78-79	0.0	0.0	0.0	3.2203465903185267	2.3622568056618572E-4
80-81	0.0	0.0	0.0	3.4531469985165026	2.3622568056618572E-4
82-83	0.0	0.0	0.0	3.6837032627491	2.3622568056618572E-4
84-85	0.0	0.0	0.0	3.910007464731506	2.3622568056618572E-4
86-87	0.0	0.0	0.0	4.134539974109666	2.3622568056618572E-4
88-89	0.0	0.0	0.0	4.384702969829256	2.3622568056618572E-4
90-91	0.0	0.0	0.0	4.645968572535457	2.3622568056618572E-4
92-93	0.0	0.0	0.0	4.902864000151185	2.3622568056618572E-4
94-95	0.0	0.0	0.0	5.18184652889985	2.3622568056618572E-4
96-97	0.0	0.0	0.0	5.445238162731147	2.3622568056618572E-4
98-99	0.0	0.0	0.0	5.741465166161143	2.3622568056618572E-4
100-101	0.0	0.0	0.0	6.056826449717002	2.3622568056618572E-4
102-103	0.0	0.0	0.0	6.382463550377489	2.3622568056618572E-4
104-105	0.0	0.0	0.0	6.728179833886101	2.3622568056618572E-4
106-107	0.0	0.0	0.0	7.084880611541042	2.3622568056618572E-4
108-109	0.0	0.0	0.0	7.442171953397398	2.3622568056618572E-4
110-111	0.0	0.0	0.0	7.810211563719515	2.3622568056618572E-4
112-113	0.0	0.0	0.0	8.203172983341364	2.3622568056618572E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1690	0.0	42.298645	1
GTATCAA	2555	0.0	37.770786	1
ATCAACG	3040	0.0	30.739592	3
TATCAAC	3095	0.0	30.577955	2
CAACGCA	3135	0.0	29.808088	5
AACGCAG	3220	0.0	28.812508	6
TCAACGC	3245	0.0	28.797642	4
ACGCAGA	3575	0.0	25.94834	7
CGCAGAG	3670	0.0	25.27964	8
CATGGGT	1400	0.0	24.233648	4
GCAGAGT	3780	0.0	24.229324	9
GTGTAAG	130	0.00203594	22.911766	1
CCGCACA	130	0.0020457758	22.892786	4
TGATCGC	130	0.0020556508	22.873837	9
CAGAGTA	3750	0.0	21.964985	10-11
GTACACT	410	1.8189894E-12	21.776064	4
GTACATG	4805	0.0	21.19994	1
TACATGG	4840	0.0	20.660265	2
TATTCTC	260	7.6451397E-7	20.603506	5
ATACCAT	175	4.7917402E-4	20.390392	6
>>END_MODULE