FastQCFastQC Report
Sun 1 Jan 2017
ERR1142855_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1142855_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences423324
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34430.8133250181893774No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30870.7292286759078153No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18080.4270960304636638No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8260.1951224121476694No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6900.16299571959066814No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5400.12756186750574028No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4530.10701023329648214No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20950.057.402691
GTATCAA34950.047.0139581
TATCAAC42650.038.7960972
ATCAACG44350.037.0361983
CAACGCA46150.036.103225
TCAACGC46700.035.937114
AACGCAG47700.034.8011976
ACGCAGA52300.031.2852337
CGCAGAG53200.030.7559688
ACCGCAT800.00535894329.7494138
GCAGAGT55800.029.3228939
CATGGGT12750.027.5392934
CTCAACG2455.475158E-1026.7295421
CAGAGTA55350.025.6914510-11
GTGGTAT11000.025.4372441
GAGTACT24900.025.08986312-13
ACCCCGG1200.001286667924.7911767
TGGTATC11800.023.7070832
ACATGGG42700.022.8574053
ATGGGTG6600.022.5400945
Produced by FastQC (version 0.11.5)