##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1142855_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	423324
Sequences flagged as poor quality	0
Sequence length	125
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.739825759938014	33.0	14.0	33.0	14.0	33.0
2	30.116775802931087	33.0	27.0	33.0	27.0	33.0
3	29.624075176460583	33.0	27.0	33.0	27.0	33.0
4	31.08403728586142	33.0	33.0	33.0	27.0	33.0
5	31.8606481087772	33.0	33.0	33.0	27.0	33.0
6	34.484661866560835	37.0	33.0	37.0	27.0	37.0
7	35.71377715414198	37.0	37.0	37.0	33.0	37.0
8	35.5687582088424	37.0	37.0	37.0	33.0	37.0
9	35.952846991902184	37.0	37.0	37.0	33.0	37.0
10-11	35.96232909072011	37.0	37.0	37.0	33.0	37.0
12-13	36.00947855543272	37.0	37.0	37.0	33.0	37.0
14-15	36.09238313915583	37.0	37.0	37.0	37.0	37.0
16-17	36.130578469446576	37.0	37.0	37.0	37.0	37.0
18-19	36.14525280872334	37.0	37.0	37.0	37.0	37.0
20-21	36.09149611172529	37.0	37.0	37.0	37.0	37.0
22-23	36.13976410503538	37.0	37.0	37.0	37.0	37.0
24-25	36.17179276393496	37.0	37.0	37.0	37.0	37.0
26-27	36.11920538405571	37.0	37.0	37.0	37.0	37.0
28-29	36.12315035292117	37.0	37.0	37.0	37.0	37.0
30-31	36.08221598586425	37.0	37.0	37.0	37.0	37.0
32-33	36.014832610482756	37.0	37.0	37.0	37.0	37.0
34-35	35.954404900265516	37.0	37.0	37.0	37.0	37.0
36-37	35.879827271782375	37.0	37.0	37.0	35.0	37.0
38-39	35.825218981205886	37.0	37.0	37.0	33.0	37.0
40-41	35.74232738989521	37.0	37.0	37.0	33.0	37.0
42-43	35.65397307972144	37.0	37.0	37.0	33.0	37.0
44-45	35.535598028932924	37.0	37.0	37.0	33.0	37.0
46-47	35.37740950194177	37.0	37.0	37.0	33.0	37.0
48-49	35.112775321030696	37.0	37.0	37.0	33.0	37.0
50-51	35.03838194857839	37.0	37.0	37.0	33.0	37.0
52-53	35.003079201746175	37.0	37.0	37.0	33.0	37.0
54-55	34.947148047358525	37.0	37.0	37.0	27.0	37.0
56-57	34.91889663709121	37.0	37.0	37.0	27.0	37.0
58-59	34.89107633869094	37.0	37.0	37.0	27.0	37.0
60-61	34.85792797006548	37.0	37.0	37.0	27.0	37.0
62-63	34.84505130821782	37.0	37.0	37.0	27.0	37.0
64-65	34.82556387069951	37.0	37.0	37.0	27.0	37.0
66-67	34.789579612778866	37.0	37.0	37.0	27.0	37.0
68-69	34.724081318328274	37.0	37.0	37.0	27.0	37.0
70-71	34.72611994595157	37.0	37.0	37.0	27.0	37.0
72-73	34.709371545199424	37.0	37.0	37.0	27.0	37.0
74-75	34.657887339248425	37.0	37.0	37.0	27.0	37.0
76-77	34.602954238361164	37.0	37.0	37.0	27.0	37.0
78-79	34.517494873902734	37.0	37.0	37.0	27.0	37.0
80-81	34.44636850261266	37.0	37.0	37.0	27.0	37.0
82-83	34.456932515047576	37.0	37.0	37.0	27.0	37.0
84-85	34.415961533010176	37.0	37.0	37.0	27.0	37.0
86-87	34.34743718759154	37.0	37.0	37.0	27.0	37.0
88-89	34.28180542563143	37.0	37.0	37.0	27.0	37.0
90-91	34.23509415955627	37.0	37.0	37.0	27.0	37.0
92-93	34.13924677079495	37.0	37.0	37.0	27.0	37.0
94-95	34.084616038778805	37.0	37.0	37.0	22.0	37.0
96-97	34.02201269004355	37.0	37.0	37.0	22.0	37.0
98-99	33.93971520631951	37.0	37.0	37.0	22.0	37.0
100-101	33.86921010856932	37.0	37.0	37.0	22.0	37.0
102-103	33.78732838204307	37.0	37.0	37.0	22.0	37.0
104-105	33.645676833819955	37.0	33.0	37.0	22.0	37.0
106-107	33.58833068760571	37.0	33.0	37.0	22.0	37.0
108-109	33.51698226417591	37.0	33.0	37.0	22.0	37.0
110-111	33.462349169902964	37.0	33.0	37.0	22.0	37.0
112-113	33.39873005074128	37.0	33.0	37.0	18.0	37.0
114-115	33.26544089161021	37.0	33.0	37.0	14.0	37.0
116-117	33.198812257278114	37.0	33.0	37.0	14.0	37.0
118-119	33.04508485226446	37.0	33.0	37.0	14.0	37.0
120-121	32.93355207831354	37.0	33.0	37.0	14.0	37.0
122-123	32.849678969300115	37.0	33.0	37.0	14.0	37.0
124-125	31.273750129924125	35.0	27.5	37.0	14.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	11.0
16	12.0
17	23.0
18	93.0
19	299.0
20	1141.0
21	2702.0
22	4215.0
23	5995.0
24	5383.0
25	4281.0
26	3815.0
27	3955.0
28	4585.0
29	5634.0
30	7574.0
31	10056.0
32	14133.0
33	21368.0
34	35790.0
35	78127.0
36	214132.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.45859544481362	17.477302051677118	13.530387602028519	25.533714901480742
2	17.059475242075127	21.100871919285456	38.635166387523356	23.20448645111606
3	19.796607767173768	26.38972881035623	29.38226400831522	24.431399414154775
4	13.931897321534034	18.29592111542114	35.561372259049094	32.21080930399573
5	14.797867289061706	35.20366822501659	32.48840686870882	17.510057617212876
6	28.127391785015732	35.10219122941293	19.190029386474663	17.58038759909667
7	25.18968922149465	33.2511267964963	22.79506949759522	18.76411448441383
8	26.056165017811416	31.3523447761053	21.402046659296424	21.18944354678686
9	27.41517135810868	16.06759834075082	21.325509538792982	35.191720762347515
10-11	25.261029377025636	25.591390991297448	26.449244550273548	22.69833508140337
12-13	26.692305239174573	24.97339486275765	26.80770243822352	21.526597459844254
14-15	24.89086321996022	24.65629001091368	26.46295255148563	23.989894217640472
16-17	22.701524128091012	26.90291596980091	27.090951611531594	23.304608290576486
18-19	22.745580217516608	24.93243945535807	30.352637695949202	21.96934263117612
20-21	24.894996692809222	23.333766417839932	30.332018331286026	21.43921855806482
22-23	24.671410078332435	23.233150022205216	30.304683882794265	21.790756016668084
24-25	23.277942282911297	24.17394734759587	30.26196277787555	22.286147591617286
26-27	22.629163211841604	24.386458503121744	30.566284214579483	22.41809407045717
28-29	22.415454828925363	24.337032627491002	30.866428551180658	22.38108399240298
30-31	23.156123027496932	23.935911367287158	30.675848058206554	22.232117547009356
32-33	22.763297143559072	24.23970764709773	30.28566771550869	22.71132749383451
34-35	22.856960625903564	24.360064631346205	30.52484621708195	22.258128525668283
36-37	22.907266808717715	24.238522921086076	30.287818729005345	22.566391541190868
38-39	22.83321994500666	24.225534106263762	30.18905141215712	22.75219453657246
40-41	22.617382758979108	24.337610673822866	30.304547902748773	22.740458664449253
42-43	22.832275042284397	24.323567763698726	29.94656575105593	22.897591442960945
44-45	22.929954361198515	24.41912105148775	29.704670654156157	22.946253933157582
46-47	22.889677882661978	24.333489242282507	29.617975829388364	23.158857045667148
48-49	22.90609556746133	24.74003363853691	29.357537016564144	22.996333777437613
50-51	22.89773660096829	24.945461331582113	29.059926982555893	23.096875084893703
52-53	22.954866508237227	24.80700270715909	28.77384118944917	23.464289595154515
54-55	22.57537961466867	24.71133221834812	29.09119256172577	23.62209560525744
56-57	22.89806389432208	24.540895389819617	28.65913579197022	23.901904923888086
58-59	22.888024302898017	24.716056731959448	28.800044410427944	23.59587455471459
60-61	23.002384701061953	24.674510155944567	29.188433904567074	23.134671238426403
62-63	22.51209772195496	24.618760829483836	29.267333375066585	23.60180807349462
64-65	22.485377630372952	24.335024709206188	29.367340382307646	23.81225727811322
66-67	22.82899484673069	24.309777274354012	29.26154583905689	23.599682039858404
68-69	22.688649828500157	24.230022394194517	29.341946121646778	23.73938165565855
70-71	22.564867571883475	24.333961693643637	29.684355245627465	23.416815488845426
72-73	22.783258213566913	24.63420453364326	29.464311024180063	23.118226228609764
74-75	22.9118830966352	24.427034611786716	29.64337009004923	23.017712201528852
76-77	22.548476519730183	24.221783104410694	29.705060078167172	23.524680297691955
78-79	22.60089198816982	24.45278321096843	29.727702658011356	23.218622142850393
80-81	22.42336838922433	24.67837873590914	29.745537696894104	23.15271517797243
82-83	22.640814128185504	24.522587899575736	29.794790751292155	23.041807220946602
84-85	22.573253583543572	24.69526887206962	29.347733650820647	23.38374389356616
86-87	22.231435023764305	24.92334476665627	29.549942833385302	23.29527737619412
88-89	22.44510115183642	24.459043191503437	29.466437055305157	23.629418601354992
90-91	22.77085636533719	24.16139883399004	29.469271763471948	23.598473037200822
92-93	22.788218952858802	24.55436025361189	29.141036180325237	23.51638461320407
94-95	22.253758350577808	24.705780914854817	29.213203125738207	23.82725760882917
96-97	22.313995899122187	24.47912237435156	29.166902892347235	24.03997883417902
98-99	22.12530384599939	24.641703852613723	28.900390481972394	24.33260181941449
100-101	22.433342861124956	25.01387829151735	28.643258221263668	23.90952062609402
102-103	22.680526831135055	24.85191585158868	28.685036384703423	23.782520932572844
104-105	22.65653882451045	24.88982959759899	28.641115558741998	23.812516019148568
106-107	22.740646337848005	24.849671704972675	28.53030838117893	23.879373576000386
108-109	22.52927732402601	25.046388982395218	28.277377176974998	24.146956516603773
110-111	22.232078105843527	25.414872331529352	28.31053356420511	24.042515998422008
112-113	22.354194571022934	25.448418575974262	28.10255101920292	24.094835833799884
114-115	22.362462528435866	25.572437937168246	27.965657267180227	24.09944226721566
116-117	22.322395626019468	25.475908972258672	28.067792446166802	24.133902955555055
118-119	22.037515842240225	25.259352089285862	28.465597091524153	24.237534976949764
120-121	22.398238680522255	25.210302396065376	28.44037239056125	23.95108653285111
122-123	22.134002657611102	25.55570648161819	28.402185146906838	23.908105713863872
124-125	22.114553263062408	25.39367939638188	28.180617697501148	24.311149643054563
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	282.0
1	280.0
2	459.0
3	604.0
4	462.0
5	410.0
6	587.0
7	1167.5
8	1914.5
9	2200.0
10	1904.5
11	1438.5
12	1227.5
13	1150.5
14	1108.0
15	1024.0
16	951.5
17	969.0
18	907.5
19	849.0
20	842.5
21	837.5
22	859.5
23	923.0
24	989.0
25	1039.0
26	1179.5
27	1403.5
28	1702.0
29	2261.5
30	3061.0
31	3779.0
32	4604.0
33	5583.5
34	6477.0
35	7197.5
36	8015.0
37	8945.5
38	9454.5
39	9895.0
40	10055.5
41	9869.5
42	10069.5
43	10761.0
44	11823.5
45	12720.0
46	13774.5
47	14965.5
48	15722.5
49	16430.5
50	17029.0
51	16822.5
52	16324.5
53	16317.0
54	16321.0
55	15982.5
56	15330.0
57	14042.0
58	12399.5
59	10845.5
60	9386.5
61	7713.0
62	5990.0
63	4673.0
64	3619.5
65	2886.0
66	2301.0
67	1783.0
68	1485.0
69	1242.0
70	997.0
71	796.0
72	660.0
73	656.0
74	599.0
75	505.0
76	393.5
77	321.0
78	263.5
79	171.5
80	113.5
81	89.0
82	83.5
83	69.5
84	52.5
85	35.0
86	19.5
87	7.5
88	1.0
89	0.0
90	0.5
91	1.0
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03732365752945734
2	0.0011811284028309286
3	9.449027222647429E-4
4	0.005196964972456086
5	0.0030709338473604146
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	8.2678988198165E-4
14-15	4.7245136113237144E-4
16-17	0.0
18-19	0.0
20-21	9.449027222647429E-4
22-23	0.0
24-25	1.1811284028309286E-4
26-27	7.086770416985572E-4
28-29	0.0
30-31	9.449027222647429E-4
32-33	0.0
34-35	0.0
36-37	4.7245136113237144E-4
38-39	0.0
40-41	0.0018898054445294857
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	1.1811284028309286E-4
52-53	4.7245136113237144E-4
54-55	0.0
56-57	0.0
58-59	0.0
60-61	1.1811284028309286E-4
62-63	1.1811284028309286E-4
64-65	0.0
66-67	1.1811284028309286E-4
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	1.1811284028309286E-4
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	2.3622568056618572E-4
100-101	2.3622568056618572E-4
102-103	1.1811284028309286E-4
104-105	5.905642014154643E-4
106-107	1.1811284028309286E-4
108-109	3.543385208492786E-4
110-111	2.3622568056618572E-4
112-113	7.086770416985572E-4
114-115	7.086770416985572E-4
116-117	5.905642014154643E-4
118-119	0.0022441439653787644
120-121	0.0011811284028309286
122-123	0.002716595326511136
124-125	0.002362256805661857
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	423324.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	72.21838792312101
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.09018397757885	62.895126907887665
2	8.644866129351287	12.486365913458817
3	1.8930882500795825	4.101473448508488
4	0.7582132132696926	2.1902774385738693
5	0.37773320240201425	1.3639641471255723
6	0.23872685310639638	1.0344281091182195
7	0.15575109741217275	0.787366522065487
8	0.12366678183829327	0.7144812499201472
9	0.0814215401058364	0.5292119131795122
>10	0.5818518780058851	7.576797285879137
>50	0.036822349224987776	1.7923349721204673
>100	0.015465386671867408	1.7641469144589539
>500	0.0011046704765619577	0.5467419439444693
>1k	0.0011046704765619577	2.2172832337592334
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3443	0.8133250181893774	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3087	0.7292286759078153	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1808	0.4270960304636638	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	826	0.1951224121476694	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	690	0.16299571959066814	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	540	0.12756186750574028	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	453	0.10701023329648214	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	2.3622568056618572E-4	0.0
2	0.0	0.0	0.0	2.3622568056618572E-4	0.0
3	0.0	0.0	0.0	2.3622568056618572E-4	0.0
4	0.0	0.0	0.0	2.3622568056618572E-4	0.0
5	0.0	0.0	0.0	2.3622568056618572E-4	0.0
6	0.0	0.0	0.0	2.3622568056618572E-4	0.0
7	0.0	0.0	0.0	2.3622568056618572E-4	0.0
8	0.0	0.0	0.0	2.3622568056618572E-4	0.0
9	0.0	0.0	0.0	2.3622568056618572E-4	0.0
10-11	0.0	0.0	0.0	4.7245136113237144E-4	0.0
12-13	2.3622568056618572E-4	0.0	0.0	7.086770416985572E-4	0.0
14-15	2.3622568056618572E-4	0.0	0.0	7.086770416985572E-4	0.0
16-17	2.3622568056618572E-4	0.0	0.0	7.086770416985572E-4	0.0
18-19	2.3622568056618572E-4	0.0	0.0	0.0011811284028309286	0.0
20-21	2.3622568056618572E-4	0.0	0.0	0.0020079182848125785	0.0
22-23	2.3622568056618572E-4	0.0	0.0	0.0042520622501913425	0.0
24-25	2.3622568056618572E-4	0.0	0.0	0.008740350180948871	0.0
26-27	2.3622568056618572E-4	0.0	0.0	0.014645992195103515	0.0
28-29	2.3622568056618572E-4	0.0	0.0	0.0264572762234128	0.0
30-31	2.3622568056618572E-4	0.0	0.0	0.04736324895352023	0.0
32-33	2.3622568056618572E-4	0.0	0.0	0.08787595317062108	0.0
34-35	2.3622568056618572E-4	0.0	0.0	0.13677466904782154	0.0
36-37	2.3622568056618572E-4	0.0	0.0	0.20563445493286467	0.0
38-39	2.3622568056618572E-4	0.0	0.0	0.3059122563332105	0.0
40-41	2.3622568056618572E-4	0.0	0.0	0.4132768281505419	0.0
42-43	2.3622568056618572E-4	0.0	0.0	0.5098931315021118	0.0
44-45	2.3622568056618572E-4	0.0	0.0	0.6022573726034905	0.0
46-47	2.3622568056618572E-4	0.0	0.0	0.7312365941926279	0.0
48-49	2.3622568056618572E-4	0.0	0.0	0.8583260103372358	0.0
50-51	2.3622568056618572E-4	0.0	0.0	0.9808090257108031	0.0
52-53	2.3622568056618572E-4	0.0	0.0	1.1074259904942787	0.0
54-55	2.3622568056618572E-4	0.0	0.0	1.2274286362219011	0.0
56-57	2.3622568056618572E-4	0.0	0.0	1.3553448422484906	0.0
58-59	2.3622568056618572E-4	0.0	0.0	1.494599880942257	0.0
60-61	2.3622568056618572E-4	0.0	0.0	1.646138655025465	0.0
62-63	2.3622568056618572E-4	0.0	0.0	1.790000094490272	0.0
64-65	2.3622568056618572E-4	0.0	0.0	1.928428343302057	0.0
66-67	2.3622568056618572E-4	0.0	0.0	2.078077311940736	0.0
68-69	2.3622568056618572E-4	0.0	0.0	2.2609159886989634	0.0
70-71	2.3622568056618572E-4	0.0	0.0	2.45403048256182	0.0
72-73	2.3622568056618572E-4	0.0	0.0	2.6384046262437284	0.0
74-75	2.3622568056618572E-4	0.0	0.0	2.8142746454252534	0.0
76-77	2.3622568056618572E-4	0.0	0.0	2.9974676607043307	0.0
78-79	2.3622568056618572E-4	0.0	0.0	3.208889644811067	0.0
80-81	2.3622568056618572E-4	0.0	0.0	3.441690053009043	0.0
82-83	2.3622568056618572E-4	0.0	0.0	3.6714195273596584	0.0
84-85	2.3622568056618572E-4	0.0	0.0	3.8965426009392337	0.0
86-87	2.3622568056618572E-4	0.0	0.0	4.1217837873590915	0.0
88-89	2.3622568056618572E-4	0.0	0.0	4.371828670238399	0.0
90-91	2.3622568056618572E-4	0.0	0.0	4.632976160104317	0.0
92-93	2.3622568056618572E-4	0.0	0.0	4.889399136358912	0.0
94-95	2.3622568056618572E-4	0.0	0.0	5.167791100906161	0.0
96-97	2.3622568056618572E-4	0.0	0.0	5.431182734737459	0.0
98-99	2.3622568056618572E-4	0.0	0.0	5.728472753730003	0.0
100-101	2.3622568056618572E-4	0.0	0.0	6.04643251977209	0.0
102-103	2.3622568056618572E-4	0.0	0.0	6.372423958953426	0.0
104-105	2.3622568056618572E-4	0.0	0.0	6.7161323241772255	0.0
106-107	2.3622568056618572E-4	0.0	0.0	7.071770086269618	0.0
108-109	2.3622568056618572E-4	0.0	0.0	7.428943315285691	0.0
110-111	2.3622568056618572E-4	0.0	0.0	7.795565571524412	0.0
112-113	2.3622568056618572E-4	0.0	0.0	8.189826232389375	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2095	0.0	57.40269	1
GTATCAA	3495	0.0	47.013958	1
TATCAAC	4265	0.0	38.796097	2
ATCAACG	4435	0.0	37.036198	3
CAACGCA	4615	0.0	36.10322	5
TCAACGC	4670	0.0	35.93711	4
AACGCAG	4770	0.0	34.801197	6
ACGCAGA	5230	0.0	31.285233	7
CGCAGAG	5320	0.0	30.755968	8
ACCGCAT	80	0.005358943	29.749413	8
GCAGAGT	5580	0.0	29.322893	9
CATGGGT	1275	0.0	27.539293	4
CTCAACG	245	5.475158E-10	26.729542	1
CAGAGTA	5535	0.0	25.69145	10-11
GTGGTAT	1100	0.0	25.437244	1
GAGTACT	2490	0.0	25.089863	12-13
ACCCCGG	120	0.0012866679	24.791176	7
TGGTATC	1180	0.0	23.707083	2
ACATGGG	4270	0.0	22.857405	3
ATGGGTG	660	0.0	22.540094	5
>>END_MODULE